Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,036,077 C→T 5.9% L20L (CTC→CTT kinB → sporulation sensor histidine kinase KinB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,036,0770CT 5.9% 326.8 / 6.9 102L20L (CTC→CTTkinBsporulation sensor histidine kinase KinB
Reads supporting (aligned to +/- strand):  ref base C (46/49);  new base T (3/3);  total (50/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 107 total aligned reads displayed.

ATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACTACATACAATACGG  >  NZ_CP034943/3035955‑3036214
                                                                                                                          |                                                                                                                                         
atTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCt                                                                                                                          <  1:1240946/140‑1 (MQ=255)
atTTTACTTATAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCt                                                                                                                          <  1:976867/140‑1 (MQ=255)
    tACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGa                                                                                                                      >  1:196590/1‑140 (MQ=255)
                 tAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGAGTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGATTGGAAATCCGGCGATcc                                                                                                         >  2:2120247/1‑140 (MQ=255)
                    tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTCTCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTtg                                                                                                      >  1:591195/1‑140 (MQ=255)
                    tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTTCTGCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTtg                                                                                                      >  1:668707/1‑140 (MQ=255)
                    tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTTCTGCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTtg                                                                                                      >  1:2374105/1‑140 (MQ=255)
                    tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTTCTGCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTtg                                                                                                      >  1:2374122/1‑140 (MQ=255)
                    tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTtg                                                                                                      >  1:722852/1‑140 (MQ=255)
                       tataATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGc                                                                                                   <  2:1085166/140‑1 (MQ=255)
                        ataataGATTCTTATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                                                  >  1:598067/1‑140 (MQ=255)
                        ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                                                  >  1:1256217/1‑140 (MQ=255)
                        ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                                                  >  1:1737361/1‑140 (MQ=255)
                        ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                                                  >  2:1500208/1‑140 (MQ=255)
                        ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                                                  >  1:1857438/1‑140 (MQ=255)
                              gATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAAt                                                                                            >  1:479822/1‑140 (MQ=255)
                              gATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAAt                                                                                            >  1:369536/1‑140 (MQ=255)
                              gATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAAt                                                                                            >  1:2399023/1‑140 (MQ=255)
                              gATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCGATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAAt                                                                                            >  1:389148/1‑140 (MQ=255)
                                ttCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAATCCGGCGATCCTTGTGCCCAAAATaa                                                                                          >  2:2008130/1‑140 (MQ=255)
                                ttCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATaa                                                                                          >  2:588294/1‑140 (MQ=255)
                                    tatTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAg                                                                                      >  2:1140911/1‑140 (MQ=255)
                                    tatTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAg                                                                                      >  2:569954/1‑140 (MQ=255)
                                      ttttATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCg                                                                                    <  2:2147085/140‑1 (MQ=255)
                                         tcttcGACAGCATTTCACAAGAATCGCAATTCAACAAGACTATCTCCTGCATATCTGATTTATTGTGTTTCCTATTCTGCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCGAAAATAAACAGCGGGt                                                                                 <  1:666624/136‑1 (MQ=255)
                                         tataCGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGt                                                                                 >  1:1960640/1‑140 (MQ=255)
                                         tataCGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGt                                                                                 >  1:834876/1‑140 (MQ=255)
                                         tataCGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGt                                                                                 >  1:1505622/1‑140 (MQ=255)
                                         tataCGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGt                                                                                 >  2:438402/1‑140 (MQ=255)
                                         tataCGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGt                                                                                 >  1:2044832/1‑140 (MQ=255)
                                             cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTCCCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                                                             <  1:733801/140‑1 (MQ=255)
                                             cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                                                             <  1:2370502/140‑1 (MQ=255)
                                             cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                                                             <  1:2139558/140‑1 (MQ=255)
                                             cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                                                             <  2:479822/140‑1 (MQ=255)
                                             cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                                                             <  2:2399023/140‑1 (MQ=255)
                                                aaGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCAc                                                                          >  1:745009/1‑140 (MQ=255)
                                                aaGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCAc                                                                          >  1:2022106/1‑140 (MQ=255)
                                                   gATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTATACCAAGGGTTTTGGCTTGGAAAGCCGGCGCTCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGtt                                                                       >  2:1244072/1‑140 (MQ=255)
                                                             gAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTgc                                                             >  2:2106584/1‑140 (MQ=255)
                                                               aTGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTgcgg                                                           <  1:914612/140‑1 (MQ=255)
                                                                    aaTTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCt                                                      <  1:2187750/140‑1 (MQ=255)
                                                                           aaaGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGtttt                                               <  2:196590/140‑1 (MQ=255)
                                                                             aGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTAt                                             <  2:722852/140‑1 (MQ=255)
                                                                              gACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATg                                            <  2:1162111/140‑1 (MQ=255)
                                                                               aCTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGc                                           <  2:1574794/140‑1 (MQ=255)
                                                                                cTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCa                                          <  1:494501/140‑1 (MQ=255)
                                                                                cTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCa                                          <  1:1102583/140‑1 (MQ=255)
                                                                                   tctcCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCgg                                                                                   >  1:1001412/1‑96 (MQ=255)
                                                                                   tctcCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCgg                                                                                   <  2:1001412/96‑1 (MQ=255)
                                                                                        tGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTc                                  >  1:550922/1‑140 (MQ=255)
                                                                                         gCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTcc                                 >  1:2039331/1‑140 (MQ=255)
                                                                                         gCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTTGTGCCAAAAATAAACAGCGGTTTGGTCACGCTCTTCGCCTGCGGCGCCTCCGTTTTATGCATTATTTTcc                                 <  2:668707/140‑1 (MQ=255)
                                                                                         gCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTTGTGCCAAAAATAAACAGCGGTTTGGTCACGCTCTTCGCCTGCGGCGCCTCCGTTTTATGCATTATTTTcc                                 <  2:2374105/140‑1 (MQ=255)
                                                                                         gCACATCTGCTCTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTTGTGCCAAAAATAAACAGCGGTTTGGTCACGCTCTTCGCCTGCGGCGCCTCCGTTTTATGCATTATTTTcc                                 <  2:2374122/140‑1 (MQ=255)
                                                                                          cATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCCAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTccc                                <  2:66822/140‑1 (MQ=255)
                                                                                          cATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTccc                                <  2:66853/140‑1 (MQ=255)
                                                                                          cATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTccc                                <  2:64825/140‑1 (MQ=255)
                                                                                          cATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTccc                                >  2:385100/1‑140 (MQ=255)
                                                                                             atCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGCGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTAt                             >  1:1190890/1‑140 (MQ=255)
                                                                                                 gCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCAt                         >  2:1149787/1‑140 (MQ=255)
                                                                                                  gtttATTTTGTTTCCTATTCTTCTCTACCCAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATg                        <  1:898419/139‑1 (MQ=255)
                                                                                                  cTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATAtt                                   >  2:1612121/1‑129 (MQ=255)
                                                                                                  cTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATAtt                                   <  1:1612121/129‑1 (MQ=255)
                                                                                                  cTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATg                        >  2:85061/1‑140 (MQ=255)
                                                                                                  cTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATg                        >  2:924649/1‑140 (MQ=255)
                                                                                                  cTTTATTTTGTTTCCTAGTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATg                        <  1:1791287/140‑1 (MQ=255)
                                                                                                    ttATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGaa                      <  1:624820/140‑1 (MQ=255)
                                                                                                    ttATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGaa                      >  2:47463/1‑140 (MQ=255)
                                                                                                    ttATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGaa                      <  1:1871173/140‑1 (MQ=255)
                                                                                                    ttATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGaa                      <  1:385100/140‑1 (MQ=255)
                                                                                                      aTTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAAt                    <  2:226221/140‑1 (MQ=255)
                                                                                                       ttttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAATCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCTCCTCCGTTTTATGCATTATATTCACTATTCATGAAATg                   >  1:170832/1‑140 (MQ=255)
                                                                                                       ttttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATg                   >  1:338467/1‑140 (MQ=255)
                                                                                                       ttttGTTTCCTATTCTTATCTACCAAGTGTTTTGGCTTGGAAATCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATg                   >  1:338402/1‑140 (MQ=255)
                                                                                                         ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                 <  1:924649/140‑1 (MQ=255)
                                                                                                         ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                 <  1:1029076/140‑1 (MQ=255)
                                                                                                         ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                 <  1:1140911/140‑1 (MQ=255)
                                                                                                         ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                 <  1:1500208/140‑1 (MQ=255)
                                                                                                         ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                 <  2:294978/140‑1 (MQ=255)
                                                                                                         ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                 <  1:85061/140‑1 (MQ=255)
                                                                                                         ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                 <  2:1865884/140‑1 (MQ=255)
                                                                                                         ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                 <  1:569954/140‑1 (MQ=255)
                                                                                                         ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                 <  1:588294/140‑1 (MQ=255)
                                                                                                         ttGTTTCCTATTCGTCTCTACCAAGTGTTTTGGCTAGGAAAGCCGGCGAGCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCTCTTGCGGCGCCTCCGTTTTATGCATTATAGTCCCGAGTCCTGAAATGGa                 <  2:1967710/140‑1 (MQ=255)
                                                                                                           gTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACt               <  2:2022106/140‑1 (MQ=255)
                                                                                                           gTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACt               <  2:745009/140‑1 (MQ=255)
                                                                                                           gTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACt               <  1:1471002/140‑1 (MQ=255)
                                                                                                           gTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACt               <  1:1764723/140‑1 (MQ=255)
                                                                                                            tttCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACta              >  1:332939/1‑140 (MQ=255)
                                                                                                            tttCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACta              >  1:1267534/1‑140 (MQ=255)
                                                                                                            tttCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACta              >  1:55686/1‑140 (MQ=255)
                                                                                                            gttCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTAGTCATGAACTGGACta              >  1:1389793/2‑140 (MQ=255)
                                                                                                              tCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACtaca            <  1:1225025/140‑1 (MQ=255)
                                                                                                              tCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACtaca            <  1:1149787/140‑1 (MQ=255)
                                                                                                               ccTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACtacat           <  1:993964/140‑1 (MQ=255)
                                                                                                                        ctctACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGaa                      >  2:1469497/1‑120 (MQ=255)
                                                                                                                        ctctACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGaa                      <  1:1469497/120‑1 (MQ=255)
                                                                                                                        ctctACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAAt                    <  2:1861342/122‑1 (MQ=255)
                                                                                                                        ctctACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAAt                    >  1:1861342/1‑122 (MQ=255)
                                                                                                                        ctctACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACTACATACAATACgg  >  1:2151184/1‑140 (MQ=255)
                                                                                                                          |                                                                                                                                         
ATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACTACATACAATACGG  >  NZ_CP034943/3035955‑3036214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: