Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,036,110 C→T 5.2% I31I (ATC→ATT kinB → sporulation sensor histidine kinase KinB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,036,1100CT 5.2% 378.0 / 6.8 115I31I (ATC→ATTkinBsporulation sensor histidine kinase KinB
Reads supporting (aligned to +/- strand):  ref base C (54/55);  new base T (3/3);  total (57/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 116 total aligned reads displayed.

TAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACTACATACAATACGGCCTTCAGATGATTCCCGTTATCAT  >  NZ_CP034943/3035972‑3036238
                                                                                                                                          |                                                                                                                                
tAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGAGTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGATTGGAAATCCGGCGATcc                                                                                                                                 >  2:2120247/1‑140 (MQ=255)
   tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTCTCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTtg                                                                                                                              >  1:591195/1‑140 (MQ=255)
   tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTTCTGCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTtg                                                                                                                              >  1:2374105/1‑140 (MQ=255)
   tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTTCTGCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTtg                                                                                                                              >  1:668707/1‑140 (MQ=255)
   tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTTCTGCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTtg                                                                                                                              >  1:2374122/1‑140 (MQ=255)
   tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTtg                                                                                                                              >  1:722852/1‑140 (MQ=255)
       ataataGATTCTTATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                                                                          >  1:598067/1‑140 (MQ=255)
       ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATGTGCTTTATTTTGTTTCCTCTTCTTCTCTACCAAGTGTTTTGGCTTGGATAGCCGGCGAGCCTTGTGcc                                                                                                                          >  1:1544879/1‑140 (MQ=255)
       ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                                                                          >  1:2147085/1‑140 (MQ=255)
       ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                                                                          >  1:1857438/1‑140 (MQ=255)
       ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                                                                          >  1:1737361/1‑140 (MQ=255)
       ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                                                                          >  2:1716852/1‑140 (MQ=255)
             gATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAAt                                                                                                                    >  1:369536/1‑140 (MQ=255)
             gATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAAt                                                                                                                    >  1:2399023/1‑140 (MQ=255)
             gATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAAt                                                                                                                    >  1:479822/1‑140 (MQ=255)
             gATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCGATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAAt                                                                                                                    >  1:389148/1‑140 (MQ=255)
               ttCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAATCCGGCGATCCTTGTGCCCAAAATaa                                                                                                                  >  2:2008130/1‑140 (MQ=255)
               ttCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATaa                                                                                                                  >  2:588294/1‑140 (MQ=255)
                   tatTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAg                                                                                                              >  2:1140911/1‑140 (MQ=255)
                   tatTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAg                                                                                                              >  2:569954/1‑140 (MQ=255)
                     ttttATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCg                                                                                                            <  2:2147085/140‑1 (MQ=255)
                        tcttcGACAGCATTTCACAAGAATCGCAATTCAACAAGACTATCTCCTGCATATCTGATTTATTGTGTTTCCTATTCTGCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCGAAAATAAACAGCGGGt                                                                                                         <  1:666624/136‑1 (MQ=255)
                        tataCGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGt                                                                                                         >  1:2044832/1‑140 (MQ=255)
                        tataCGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGt                                                                                                         >  2:438402/1‑140 (MQ=255)
                        tataCGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGt                                                                                                         >  1:834876/1‑140 (MQ=255)
                            cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTCCCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                                                                                     <  1:733801/140‑1 (MQ=255)
                            cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                                                                                     <  1:2139558/140‑1 (MQ=255)
                            cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                                                                                     <  2:2399023/140‑1 (MQ=255)
                            cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                                                                                     <  2:479822/140‑1 (MQ=255)
                               aaGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCAc                                                                                                  >  1:745009/1‑140 (MQ=255)
                               aaGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCAc                                                                                                  >  1:2022106/1‑140 (MQ=255)
                                  gATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTATACCAAGGGTTTTGGCTTGGAAAGCCGGCGCTCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGtt                                                                                               >  2:1244072/1‑140 (MQ=255)
                                            gAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTgc                                                                                     >  2:2106584/1‑140 (MQ=255)
                                              aTGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTgcgg                                                                                   <  1:914532/140‑1 (MQ=255)
                                              aTGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTgcgg                                                                                   <  1:914612/140‑1 (MQ=255)
                                                   aaTTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCt                                                                              <  1:2187750/140‑1 (MQ=255)
                                                            aGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTAt                                                                     <  2:722852/140‑1 (MQ=255)
                                                             gACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATg                                                                    <  2:1162111/140‑1 (MQ=255)
                                                              aCTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGc                                                                   <  2:1574794/140‑1 (MQ=255)
                                                               cTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCa                                                                  <  1:1102583/140‑1 (MQ=255)
                                                               cTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCa                                                                  <  1:494501/140‑1 (MQ=255)
                                                                  tctcCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCgg                                                                                                           >  1:1001412/1‑96 (MQ=255)
                                                                  tctcCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCgg                                                                                                           <  2:1001412/96‑1 (MQ=255)
                                                                        gCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTcc                                                         >  1:2039331/1‑140 (MQ=255)
                                                                        gCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTTGTGCCAAAAATAAACAGCGGTTTGGTCACGCTCTTCGCCTGCGGCGCCTCCGTTTTATGCATTATTTTcc                                                         <  2:668707/140‑1 (MQ=255)
                                                                        gCACATCTGCTCTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTTGTGCCAAAAATAAACAGCGGTTTGGTCACGCTCTTCGCCTGCGGCGCCTCCGTTTTATGCATTATTTTcc                                                         <  2:2374122/140‑1 (MQ=255)
                                                                         cATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCCAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTccc                                                        <  2:66822/140‑1 (MQ=255)
                                                                         cATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTccc                                                        <  2:64825/140‑1 (MQ=255)
                                                                         cATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTccc                                                        >  2:385100/1‑140 (MQ=255)
                                                                            atCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGCGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTAt                                                     >  1:1190890/1‑140 (MQ=255)
                                                                                gCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCAt                                                 >  2:1149787/1‑140 (MQ=255)
                                                                                 gtttATTTTGTTTCCTATTCTTCTCTACCCAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATg                                                <  1:898419/139‑1 (MQ=255)
                                                                                 cTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATAtt                                                           <  1:1612121/129‑1 (MQ=255)
                                                                                 cTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATAtt                                                           >  2:1612121/1‑129 (MQ=255)
                                                                                 cTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATg                                                >  2:85061/1‑140 (MQ=255)
                                                                                 cTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATg                                                >  2:924649/1‑140 (MQ=255)
                                                                                   ttATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGaa                                              <  1:624820/140‑1 (MQ=255)
                                                                                   ttATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGaa                                              <  1:1871173/140‑1 (MQ=255)
                                                                                   ttATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGaa                                              <  1:385100/140‑1 (MQ=255)
                                                                                     aTTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAAt                                            <  2:226221/140‑1 (MQ=255)
                                                                                      ttttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAATCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCTCCTCCGTTTTATGCATTATATTCACTATTCATGAAATg                                           >  1:170832/1‑140 (MQ=255)
                                                                                      ttttGTTTCCTATTCTTATCTACCAAGTGTTTTGGCTTGGAAATCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATg                                           >  1:338402/1‑140 (MQ=255)
                                                                                        ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                                         <  1:924649/140‑1 (MQ=255)
                                                                                        ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                                         <  1:1029076/140‑1 (MQ=255)
                                                                                        ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                                         <  1:1140911/140‑1 (MQ=255)
                                                                                        ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                                         <  1:1500208/140‑1 (MQ=255)
                                                                                        ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                                         <  2:304886/140‑1 (MQ=255)
                                                                                        ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                                         <  2:294978/140‑1 (MQ=255)
                                                                                        ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                                         <  2:1865884/140‑1 (MQ=255)
                                                                                        ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                                         <  1:588294/140‑1 (MQ=255)
                                                                                        ttGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGa                                         <  1:569954/140‑1 (MQ=255)
                                                                                        ttGTTTCCTATTCGTCTCTACCAAGTGTTTTGGCTAGGAAAGCCGGCGAGCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCTCTTGCGGCGCCTCCGTTTTATGCATTATAGTCCCGAGTCCTGAAATGGa                                         <  2:1967710/140‑1 (MQ=255)
                                                                                          gTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACt                                       <  1:1471002/140‑1 (MQ=255)
                                                                                          gTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACt                                       <  2:745009/140‑1 (MQ=255)
                                                                                          gTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACt                                       <  2:2022106/140‑1 (MQ=255)
                                                                                          gTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACt                                       <  1:1764723/140‑1 (MQ=255)
                                                                                           tttCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACta                                      >  1:55686/1‑140 (MQ=255)
                                                                                           tttCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACta                                      >  1:332939/1‑140 (MQ=255)
                                                                                           tttCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACta                                      >  1:1267534/1‑140 (MQ=255)
                                                                                           gttCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTAGTCATGAACTGGACta                                      >  1:1389793/2‑140 (MQ=255)
                                                                                             tCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACtaca                                    <  1:1225025/140‑1 (MQ=255)
                                                                                             tCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACtaca                                    <  1:1149787/140‑1 (MQ=255)
                                                                                              ccTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACtacat                                   <  1:993964/140‑1 (MQ=255)
                                                                                                       ctctACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGaa                                              <  1:1469497/120‑1 (MQ=255)
                                                                                                       ctctACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGaa                                              >  2:1469497/1‑120 (MQ=255)
                                                                                                       ctctACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAAt                                            >  1:1861342/1‑122 (MQ=255)
                                                                                                       ctctACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAAt                                            <  2:1861342/122‑1 (MQ=255)
                                                                                                       ctctACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACTACATACAATACgg                          >  1:2151184/1‑140 (MQ=255)
                                                                                                           aCCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACTACATACAATACGGCCtt                      <  1:1939974/140‑1 (MQ=255)
                                                                                                               aGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACtaca                                    <  1:820799/122‑1 (MQ=255)
                                                                                                               aGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACtaca                                    <  1:189727/122‑1 (MQ=255)
                                                                                                               aGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACtaca                                    >  2:820799/1‑122 (MQ=255)
                                                                                                               aGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACtaca                                    >  2:189727/1‑122 (MQ=255)
                                                                                                               aGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACTACATACAATACGGCCTTCAga                  >  1:2387659/1‑140 (MQ=255)
                                                                                                               aGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACTACATACAATACGGCCTTCAga                  >  1:1869552/1‑140 (MQ=255)
                                                                                                                      tGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACTACATACAATACGGCCTTCAGATGATTcc           >  2:650457/1‑140 (MQ=255)
                                                                                                                           tGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGttt                                                                        <  1:1328938/74‑1 (MQ=255)
                                                                                                                           tGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGttt                                                                        >  2:1328938/1‑74 (MQ=255)
                                                                                                                           tGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACTACATACAATACGGCCTTCAGATGATTCCCGTTa      >  1:1652343/1‑140 (MQ=255)
                                                                                                                               aaGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACTACATACAATACGGCCTTCAGATGATTCCCGTTATCAt  <  1:47463/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                
TAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCCTATTCATGAAATGGACTACATACAATACGGCCTTCAGATGATTCCCGTTATCAT  >  NZ_CP034943/3035972‑3036238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: