Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,122,377 A→G 5.3% intergenic (‑34/+134) EO946_RS16130 ← / ← cotNH homoserine dehydrogenase/spore coat‑associated protein CotNH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,122,3770AG 5.3% 437.2 / 9.3 135intergenic (‑34/+134)EO946_RS16130/cotNHhomoserine dehydrogenase/spore coat‑associated protein CotNH
Reads supporting (aligned to +/- strand):  ref base A (42/85);  new base G (4/3);  total (47/88)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 138 total aligned reads displayed.

ACAGCCGACCTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGACAAG  >  NZ_CP034943/3122239‑3122505
                                                                                                                                          |                                                                                                                                
aCAGCCGACCTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGaa                                                                                                                                 <  1:2179518/140‑1 (MQ=255)
aCAGCCGACCTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGaa                                                                                                                                 <  1:1585428/140‑1 (MQ=255)
    ccGACCTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAg                                                                                                                             >  2:1358623/1‑140 (MQ=255)
    ccGACCTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAg                                                                                                                             >  1:1807937/1‑140 (MQ=255)
    ccGACCTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAg                                                                                                                             >  2:1030687/1‑140 (MQ=255)
     cGACCTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCGAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGa                                                                                                                            <  1:2272342/140‑1 (MQ=255)
       aCCTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTCAACCTAAAAGCCCGACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACa                                                                                                                          <  1:1663501/140‑1 (MQ=255)
       aCCTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTCCACGAATTGCTTTCAAAAAAACTCCACCTTTCGTTTGATTGTCCTCTTGAAAAAGACa                                                                                                                          <  1:1795784/140‑1 (MQ=255)
       aCCTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACa                                                                                                                          <  1:2096512/140‑1 (MQ=255)
       aCCTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACa                                                                                                                          <  1:172129/140‑1 (MQ=255)
       aCCTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACa                                                                                                                          <  1:2195369/140‑1 (MQ=255)
         cTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAAt                                                                                                                        >  2:797867/1‑140 (MQ=255)
         cTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAAt                                                                                                                        >  2:417622/1‑140 (MQ=255)
            aTGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGt                                                                                                                     <  1:1183255/140‑1 (MQ=255)
             tGCATCAGCTTATCCTGATGTTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAACGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGtt                                                                                                                    <  1:1727961/140‑1 (MQ=255)
                 tCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAAcc                                                                                                                <  2:1636648/140‑1 (MQ=255)
                 tCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTGTCTTTTGATTGTCGTCTTGAACAAGACACTTGTTAAcc                                                                                                                >  2:513823/1‑140 (MQ=255)
                  cAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAAccc                                                                                                               <  1:854245/140‑1 (MQ=255)
                     cTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAac                                                                                                            >  2:626367/1‑140 (MQ=255)
                        aTCCTGATTGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAAcaca                                                                                                         <  1:2134991/140‑1 (MQ=255)
                        aTCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAAcaca                                                                                                         <  1:2414211/140‑1 (MQ=255)
                        aTCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAAcaca                                                                                                         <  1:1170407/140‑1 (MQ=255)
                        aTCCTGATGGTCTTGAAGAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCGTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAAcaca                                                                                                         <  2:714820/140‑1 (MQ=255)
                         tCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAg                                                                                                        >  2:1577855/1‑140 (MQ=255)
                              aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACa                                                                                                                          <  2:2355022/117‑1 (MQ=255)
                              aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACa                                                                                                                          >  1:2355022/1‑117 (MQ=255)
                              aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACata                                                                                                   >  2:11712/1‑140 (MQ=255)
                              aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACata                                                                                                   >  1:2394950/1‑140 (MQ=255)
                              aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATCGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGGAAAAGACAATTGTTAACTCAACACAGACata                                                                                                   >  1:218863/1‑140 (MQ=255)
                              aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATCGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGGAAAAGACAATTGTTAACTCAACACAGACata                                                                                                   >  1:381201/1‑140 (MQ=255)
                              aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATCGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGGAAAAGACAATTGTTAACTCAACACAGACata                                                                                                   >  1:1943782/1‑140 (MQ=255)
                              aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATCGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGGAAAAGACAATTGTTAACTCAACACAGACata                                                                                                   >  1:1593992/1‑140 (MQ=255)
                               tGGTCTTTAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTACAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACatat                                                                                                  <  2:1961093/140‑1 (MQ=255)
                               tGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACTAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACatat                                                                                                  >  2:52785/1‑140 (MQ=255)
                               tGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTTTTAACCCAACACAGACatat                                                                                                  >  2:2332240/1‑140 (MQ=255)
                               tGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACatat                                                                                                  >  2:1810340/1‑140 (MQ=255)
                               tGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACatat                                                                                                  >  2:616353/1‑140 (MQ=255)
                               tGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCGTGTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACctat                                                                                                  >  2:1425180/1‑140 (MQ=255)
                               tGGTATTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACatat                                                                                                  <  1:1590199/140‑1 (MQ=255)
                                    ttGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGtt                                                                                             >  2:205773/1‑140 (MQ=255)
                                      gAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTAc                                                                                           <  2:2320382/140‑1 (MQ=255)
                                      gAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTAc                                                                                           <  2:2320322/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTACACGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTTTTAACCCAACACAGACATATAAGTTACa                                                                                          <  2:898103/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  2:408786/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  2:2168144/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  2:1311919/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  1:612859/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  1:1114096/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  1:370809/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  1:1955818/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  1:1912927/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  1:1781035/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  1:1739813/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  1:2178930/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  1:1032925/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACGGTACCTAAAACTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  1:2021549/140‑1 (MQ=255)
                                       aataatTTTAACGACACCGCTTCCGACAGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                          <  1:881058/140‑1 (MQ=255)
                                         taatTTTAACGACACCGCTTCCGACTGTACCTAAACCTCAAAGCCCTACACGAAGTGCTTTCAAAAAAACTCCACCTTTCTTGTGATTGTCCTCTTGAAAAAGACCATTGTTAACCCAACACAGACATATAAGTTACaaa                                                                                        <  2:1143242/140‑1 (MQ=255)
                                         taatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACaaa                                                                                        <  1:353951/140‑1 (MQ=255)
                                         taatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACaaa                                                                                        <  1:1476591/140‑1 (MQ=255)
                                         taatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACaaa                                                                                        <  2:761597/140‑1 (MQ=255)
                                         taatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACaaa                                                                                        <  1:354023/140‑1 (MQ=255)
                                         taatTTTAACGACACCGCTTCCGACGGTACATAAACCTAACAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACaaa                                                                                        <  2:2375862/140‑1 (MQ=255)
                                           atTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACa                                                                                      <  1:797867/140‑1 (MQ=255)
                                           atTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACa                                                                                      <  1:1279519/140‑1 (MQ=255)
                                           atTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACa                                                                                      <  2:1489541/140‑1 (MQ=255)
                                           atTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTACACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACa                                                                                      <  1:417622/140‑1 (MQ=255)
                                           atTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATCGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGGAAAAGACAATTGTTAACTCAACACAGACATATAAGTTACAAACa                                                                                      <  1:709403/140‑1 (MQ=255)
                                           atTTTAACGACACCGCTTCCGACGGTACCAAAACCTAAAAGCCCTACACGAATCGCTTTCAAAAAAACTCCCCCTTTCTTTTGATTGTCCTCTTGGAAAAGACAATTGTTAACTCCACACAGACATATAAGTTACAAACa                                                                                      <  1:2357679/140‑1 (MQ=255)
                                            ttttAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACAt                                                                                     >  2:42546/1‑140 (MQ=255)
                                                 aCGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATtatat                                                                                <  1:358836/140‑1 (MQ=255)
                                                      acCGCTTCCGACTGTTCCTAAAACTAAAACCCCTACACGAATTGATTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCACTTGAAAAAGAGTATTGTTAAACCCACACAGACATATAAGTTACAAACATTATATAAGTc                                                                           <  1:1696107/140‑1 (MQ=255)
                                                      acCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCATAAAAACTCCACCTGGCTTTTGATTGTCGTGTTGTAATAGACAGTTGTTATCCCAAGACAGACATATAAGTTACAACCCTTTTATAAGTc                                                                           >  1:1156365/1‑140 (MQ=255)
                                                      acCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTc                                                                           <  1:2039822/140‑1 (MQ=255)
                                                                aCGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGtt                                                                 >  1:704796/1‑140 (MQ=255)
                                                                  ggTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGtttt                                                               <  2:109981/140‑1 (MQ=255)
                                                                    tACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGa                                                             <  1:205773/140‑1 (MQ=255)
                                                                     acctaaacctaaAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACaaa                                                                                        >  1:1134481/1‑112 (MQ=255)
                                                                     acctaaacctaaAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGAc                                                            >  2:644820/1‑140 (MQ=255)
                                                                      cctaaacctaccAGCCCTCCACGAATTGCTTTCAAAAAAACGCCACCTGTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCACCACAGACATATAAGTTACAACCATTATATAAGGCCACTCCTGTTTTGACa                                                           <  2:1880440/140‑1 (MQ=255)
                                                                                aaaGCCCTACACGTCTTGCTTTCAAAAAAACTCCTCCTGGCTTTTGAGTGTCCTCGTGAAACAGACCATTGTTACCCCACCACCGACATATAAGGTGCAAGCATGATATAAGGCGACTCCGGTTTTGTCAGGACAGTaag                                                 >  2:2419567/1‑139 (MQ=255)
                                                                                aaaGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAAGATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAAt                                                 >  1:1918685/1‑140 (MQ=255)
                                                                                aaaGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAAt                                                 >  2:1680810/1‑140 (MQ=255)
                                                                                       tACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGa                                          >  2:852473/1‑140 (MQ=255)
                                                                                       tACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGa                                          >  1:1646087/1‑140 (MQ=255)
                                                                                              aTTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTAt                                   >  1:1477481/1‑140 (MQ=255)
                                                                                              aTTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTAt                                   >  2:2073143/1‑140 (MQ=255)
                                                                                               ttGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATg                                  <  1:755089/140‑1 (MQ=255)
                                                                                                    ttCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGt                             >  1:117621/1‑140 (MQ=255)
                                                                                                     tCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTa                            <  1:974315/140‑1 (MQ=255)
                                                                                                     tCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTa                            <  2:1977388/140‑1 (MQ=255)
                                                                                                      cAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTaa                           <  2:1783577/140‑1 (MQ=255)
                                                                                                      cAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTaa                           <  2:758397/140‑1 (MQ=255)
                                                                                                           aaaCTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCt                      <  1:163955/140‑1 (MQ=255)
                                                                                                                 cACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAAccc                >  1:2161561/1‑140 (MQ=255)
                                                                                                                        ctttTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGata         <  1:2304855/140‑1 (MQ=255)
                                                                                                                        ctttTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGata         <  1:1123185/140‑1 (MQ=255)
                                                                                                                           ttGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCTGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGa      >  1:1604157/1‑140 (MQ=255)
                                                                                                                             gATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGACa    <  2:704796/140‑1 (MQ=255)
                                                                                                                               ttGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGACAAg  <  2:117621/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                
ACAGCCGACCTGATGCATCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGACAAG  >  NZ_CP034943/3122239‑3122505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: