Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,122,395 C→T 5.7% intergenic (‑52/+116) EO946_RS16130 ← / ← cotNH homoserine dehydrogenase/spore coat‑associated protein CotNH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,122,3950CT 5.7% 399.7 / 8.9 123intergenic (‑52/+116)EO946_RS16130/cotNHhomoserine dehydrogenase/spore coat‑associated protein CotNH
Reads supporting (aligned to +/- strand):  ref base C (38/78);  new base T (4/3);  total (42/81)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 129 total aligned reads displayed.

TCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGACAAGATTCTTTATTTTCTAATTATTTTTCCGCC  >  NZ_CP034943/3122256‑3122534
                                                                                                                                           |                                                                                                                                           
tCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAAcc                                                                                                                                             <  2:1636648/140‑1 (MQ=255)
tCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTGTCTTTTGATTGTCGTCTTGAACAAGACACTTGTTAAcc                                                                                                                                             >  2:513823/1‑140 (MQ=255)
    cTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAac                                                                                                                                         >  2:626367/1‑140 (MQ=255)
       aTCCTGATTGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAAcaca                                                                                                                                      <  1:2134991/140‑1 (MQ=255)
       aTCCTGATGTTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAAcaca                                                                                                                                      <  2:725049/140‑1 (MQ=255)
       aTCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAAcaca                                                                                                                                      <  1:1170407/140‑1 (MQ=255)
       aTCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAAcaca                                                                                                                                      <  1:2414211/140‑1 (MQ=255)
       aTCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAAcaca                                                                                                                                      <  1:361731/140‑1 (MQ=255)
       aTCCTGATGGTCTTGAAGAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCGTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAAcaca                                                                                                                                      <  2:714820/140‑1 (MQ=255)
        tCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTGTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAg                                                                                                                                     >  2:2070094/1‑140 (MQ=255)
        tCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAg                                                                                                                                     >  2:1595020/1‑140 (MQ=255)
             aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACata                                                                                                                                >  1:2394950/1‑140 (MQ=255)
             aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACata                                                                                                                                >  1:1705831/1‑140 (MQ=255)
             aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACata                                                                                                                                >  2:11712/1‑140 (MQ=255)
             aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATCGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGGAAAAGACAATTGTTAACTCAACACAGACata                                                                                                                                >  1:218863/1‑140 (MQ=255)
             aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATCGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGGAAAAGACAATTGTTAACTCAACACAGACata                                                                                                                                >  1:381201/1‑140 (MQ=255)
             aTGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATCGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGGAAAAGACAATTGTTAACTCAACACAGACata                                                                                                                                >  1:1943782/1‑140 (MQ=255)
              tGGTCTTTAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTACAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACatat                                                                                                                               <  2:1961093/140‑1 (MQ=255)
              tGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACTAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACatat                                                                                                                               >  2:52785/1‑140 (MQ=255)
              tGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTTTTAACCCAACACAGACatat                                                                                                                               >  2:2332240/1‑140 (MQ=255)
              tGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACatat                                                                                                                               >  2:285591/1‑140 (MQ=255)
              tGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACatat                                                                                                                               <  1:2182432/140‑1 (MQ=255)
              tGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACatat                                                                                                                               >  2:616353/1‑140 (MQ=255)
              tGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCGTGTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACctat                                                                                                                               >  2:1425180/1‑140 (MQ=255)
              tGGTATTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACatat                                                                                                                               <  1:1590199/140‑1 (MQ=255)
                   ttGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGtt                                                                                                                          >  2:205773/1‑140 (MQ=255)
                     gAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTAc                                                                                                                        <  2:1906063/140‑1 (MQ=255)
                     gAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTAc                                                                                                                        <  2:2320322/140‑1 (MQ=255)
                     gAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTAc                                                                                                                        <  2:2320382/140‑1 (MQ=255)
                      aattatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCACTTGAACAAGACAAGTGTTAACCCTACACAGACATATAAGTTTCa                                                                                                                       <  1:405255/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTACACGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTTTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  2:898103/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  2:1311919/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  1:1955818/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  1:160292/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  1:1558682/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  2:408786/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  1:956075/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  1:802407/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  1:1032925/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  1:612859/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  2:2168144/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  1:1799535/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  1:1781035/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  1:1114096/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  1:1739813/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACGGTACCTAAAACTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  1:2021549/140‑1 (MQ=255)
                      aataatTTTAACGACACCGCTTCCGACAGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACa                                                                                                                       <  1:881058/140‑1 (MQ=255)
                        taatTTTAACGACACCGCTTCCGACTGTACCTAAACCTCAAAGCCCTACACGAAGTGCTTTCAAAAAAACTCCACCTTTCTTGTGATTGTCCTCTTGAAAAAGACCATTGTTAACCCAACACAGACATATAAGTTACaaa                                                                                                                     <  2:1143242/140‑1 (MQ=255)
                        taatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACaaa                                                                                                                     <  1:354023/140‑1 (MQ=255)
                        taatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACaaa                                                                                                                     <  1:353951/140‑1 (MQ=255)
                        taatTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACaaa                                                                                                                     <  1:1476591/140‑1 (MQ=255)
                        taatTTTAACGACACCGCTTCCGACGGTACATAAACCTAACAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACaaa                                                                                                                     <  2:2375862/140‑1 (MQ=255)
                          atTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACa                                                                                                                   <  2:1489541/140‑1 (MQ=255)
                          atTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACa                                                                                                                   <  1:1279519/140‑1 (MQ=255)
                          atTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACa                                                                                                                   <  1:797867/140‑1 (MQ=255)
                          atTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTACACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACa                                                                                                                   <  1:417622/140‑1 (MQ=255)
                          atTTTAACGACACCGCTTCCGACGGTACCAAAACCTAAAAGCCCTACACGAATCGCTTTCAAAAAAACTCCCCCTTTCTTTTGATTGTCCTCTTGGAAAAGACAATTGTTAACTCCACACAGACATATAAGTTACAAACa                                                                                                                   <  1:2357679/140‑1 (MQ=255)
                          atTTTAACCCCACCGCTTCCCACGGTAACGAAACCCAAAATCCCTACAAGAATTGCTTTCAAAAAAACTCCAACTTTCTTTTGATTGTCCTCTTGAAAAAGAAAATTGTTAACCCAACACAGACATATAAGTTACAAACa                                                                                                                   <  1:216162/140‑1 (MQ=255)
                           ttttAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAAcaca                                                                                                                                      <  1:1245049/120‑1 (MQ=255)
                           ttttAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACAt                                                                                                                  >  2:42546/1‑140 (MQ=255)
                                aCGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATtatat                                                                                                             <  1:358836/140‑1 (MQ=255)
                                aCGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATtatat                                                                                                             <  2:799481/140‑1 (MQ=255)
                                     acCGCTTCCGACTGTTCCTAAAACTAAAACCCCTACACGAATTGATTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCACTTGAAAAAGAGTATTGTTAAACCCACACAGACATATAAGTTACAAACATTATATAAGTc                                                                                                        <  1:1696107/140‑1 (MQ=255)
                                     acCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCATAAAAACTCCACCTGGCTTTTGATTGTCGTGTTGTAATAGACAGTTGTTATCCCAAGACAGACATATAAGTTACAACCCTTTTATAAGTc                                                                                                        >  1:1156365/1‑140 (MQ=255)
                                     acCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTc                                                                                                        <  1:2039822/140‑1 (MQ=255)
                                               aCGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGtt                                                                                              >  1:704796/1‑140 (MQ=255)
                                                 ggTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGtttt                                                                                            <  2:109981/140‑1 (MQ=255)
                                                   tACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGa                                                                                          <  1:205773/140‑1 (MQ=255)
                                                    acctaaacctaaAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACaaa                                                                                                                     >  1:1134481/1‑112 (MQ=255)
                                                    acctaaacctaaAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGAc                                                                                         >  2:644820/1‑140 (MQ=255)
                                                     cctaaacctaccAGCCCTCCACGAATTGCTTTCAAAAAAACGCCACCTGTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCACCACAGACATATAAGTTACAACCATTATATAAGGCCACTCCTGTTTTGACa                                                                                        <  2:1880440/140‑1 (MQ=255)
                                                               aaaGCCCTACACGTCTTGCTTTCAAAAAAACTCCTCCTGGCTTTTGAGTGTCCTCGTGAAACAGACCATTGTTACCCCACCACCGACATATAAGGTGCAAGCATGATATAAGGCGACTCCGGTTTTGTCAGGACAGTaag                                                                              >  2:2419567/1‑139 (MQ=255)
                                                               aaaGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAAGATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAAt                                                                              >  1:1918685/1‑140 (MQ=255)
                                                               aaaGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAAt                                                                              >  1:275658/1‑140 (MQ=255)
                                                               aaaGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAAt                                                                              >  1:1918728/1‑140 (MQ=255)
                                                               aaaGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAAt                                                                              >  2:1680810/1‑140 (MQ=255)
                                                               aaaGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTGTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAAt                                                                              >  2:649822/1‑140 (MQ=255)
                                                                      tACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGa                                                                       >  1:1646087/1‑140 (MQ=255)
                                                                      tACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGa                                                                       >  2:852473/1‑140 (MQ=255)
                                                                             aTTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTAt                                                                >  2:2073143/1‑140 (MQ=255)
                                                                             aTTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTAt                                                                >  1:1477481/1‑140 (MQ=255)
                                                                              ttGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATg                                                               <  1:755089/140‑1 (MQ=255)
                                                                                   ttCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGt                                                          >  1:117621/1‑140 (MQ=255)
                                                                                    tCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTa                                                         <  1:974315/140‑1 (MQ=255)
                                                                                     cAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTaa                                                        <  2:758397/140‑1 (MQ=255)
                                                                                     cAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTaa                                                        <  2:1783577/140‑1 (MQ=255)
                                                                                          aaaCTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCt                                                   <  1:163955/140‑1 (MQ=255)
                                                                                           aaCGCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCtt                                                  <  1:705567/140‑1 (MQ=255)
                                                                                                cACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAAccc                                             >  1:2161561/1‑140 (MQ=255)
                                                                                                       ctttTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGata                                      <  1:2304855/140‑1 (MQ=255)
                                                                                                       ctttTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGata                                      <  1:1123185/140‑1 (MQ=255)
                                                                                                          ttGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCTGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGa                                   >  1:1604157/1‑140 (MQ=255)
                                                                                                              ttGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGACAAg                               <  2:117621/140‑1 (MQ=255)
                                                                                                                                cAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCCTTGATATGACAAGATTCTTTATTTTCTAatt             <  1:1425180/140‑1 (MQ=255)
                                                                                                                                cAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGACAAGATTCTTTATTTTCTAatt             <  1:616353/140‑1 (MQ=255)
                                                                                                                                cAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGACAAGATTCTTTATTTTCTAatt             <  1:1810340/140‑1 (MQ=255)
                                                                                                                                      ttAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGACAAGATTCTTTATTTTCTATTATTTTTc      <  1:141206/140‑1 (MQ=255)
                                                                                                                                           ccAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGACAAGATTCTTTATTTTCTAATTATTTTTCCGcc  <  1:1358623/140‑1 (MQ=255)
                                                                                                                                           ccAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGACAAGATTCTTTATTTTCTAATTATTTTTCCGcc  <  1:1370531/140‑1 (MQ=255)
                                                                                                                                           ccAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGACAAGATTCTTTATTTTCTAATTATTTTTCCGcc  <  1:1030687/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TCAGCTTATCCTGATGGTCTTGAATAATTTTAACGACACCGCTTCCGACGGTACCTAAACCTAAAAGCCCTACACGAATTGCTTTCAAAAAAACTCCACCTTTCTTTTGATTGTCCTCTTGAAAAAGACAATTGTTAACCCAACACAGACATATAAGTTACAAACATTATATAAGTCCACTCCTGTTTTGACAAGACAGTAATCGGCTGAATTTTATGAATGTAAACGCTTAACCCATTGATATGACAAGATTCTTTATTTTCTAATTATTTTTCCGCC  >  NZ_CP034943/3122256‑3122534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: