Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,180,566 C→T 6.1% intergenic (‑48/‑127) EO946_RS16450 ← / → EO946_RS16455 3‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,180,5660CT 6.1% 307.2 / 7.2 98intergenic (‑48/‑127)EO946_RS16450/EO946_RS164553‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein
Reads supporting (aligned to +/- strand):  ref base C (55/36);  new base T (3/3);  total (59/39)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

CAGGAATTCCGATGTTAGCCAAATGCGCCGCAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGC  >  NZ_CP034943/3180428‑3180699
                                                                                                                                          |                                                                                                                                       
caggaATTCCGATGTTAGCCAAATGCGCCGCAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCGTGAACATTGAATGAATAGTCATTCATTTTTTGACCa                                                                                                                                       >  2:2413699/1‑140 (MQ=255)
   gaATTCCGATGTTAGCCAAATGCGCCGCAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCaaaa                                                                                                                                    >  1:8039/1‑140 (MQ=255)
        ccGATGTTAGCCAAATGCGCCGCAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTTAATGAATAGTCATTCATTTTTTGACCAAAAAAAAt                                                                                                                               >  2:2026939/1‑140 (MQ=255)
        ccGATGTTAGCCAAATGCGCCGCAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGACTGAATAGTCATTCATTTTTTGACCAAAAAAAAt                                                                                                                               >  2:1922318/1‑140 (MQ=255)
        ccGATGTTAGCCAAATGCGCCGCAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAAt                                                                                                                               >  2:425546/1‑140 (MQ=255)
                          gccgcAATCCCGGAACCCATTACCCCCGATCCTACAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCttt                                                                                                             <  2:681377/140‑1 (MQ=255)
                          gccgcAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCttt                                                                                                             <  2:957230/140‑1 (MQ=255)
                          gccgcAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCttt                                                                                                             <  2:933942/140‑1 (MQ=255)
                          gccgcAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCttt                                                                                                             <  2:681683/140‑1 (MQ=255)
                          gccgcAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCttt                                                                                                             <  2:361931/140‑1 (MQ=255)
                            cgcAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCa                                                                                                           >  1:2400326/1‑140 (MQ=255)
                                    cGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTaaaa                                                                                                   <  1:287917/140‑1 (MQ=255)
                                      gAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGa                                                                                                 >  2:1223777/1‑140 (MQ=255)
                                             ttACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCgg                                                                                          >  2:30701/1‑140 (MQ=255)
                                             ttACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCgg                                                                                          >  2:1577543/1‑140 (MQ=255)
                                              tACCCCCGATCCTAAAACGGCTGCCGTCCGAATGTGTTTGTGCATGAGATAACCCCCTTGAACATTGAATGAATAGACATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGt                                                                                         <  1:1952606/140‑1 (MQ=255)
                                                cccccGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTcc                                                                                       <  1:836158/140‑1 (MQ=255)
                                                  cccGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGa                                                                                     <  1:1938842/140‑1 (MQ=255)
                                                   ccGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGAc                                                                                    <  2:8039/140‑1 (MQ=255)
                                                    cGATCCTAAAACGGCTGCCTTCCGACTGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCAGGTTAAAAGATGTCCGGTCCGACt                                                                                   >  2:2221037/1‑140 (MQ=255)
                                                    cGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACt                                                                                   >  1:2093840/1‑140 (MQ=255)
                                                    cGAACCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTATTTCATCTTAAAATATGTCCGGTCCGACt                                                                                   >  2:2039892/1‑140 (MQ=255)
                                                      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCGCCTTGAACATTGAATGAATCGTCTTTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                 >  2:2390610/1‑140 (MQ=255)
                                                      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACtt                                                                                  >  1:1624140/1‑140 (MQ=255)
                                                      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                 >  2:2082806/1‑140 (MQ=255)
                                                      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                 >  1:821493/1‑140 (MQ=255)
                                                      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                 >  1:798572/1‑140 (MQ=255)
                                                      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                 >  1:303157/1‑140 (MQ=255)
                                                      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTAGACCAAAAAAAAAGAAAATCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                 >  2:1738982/1‑140 (MQ=255)
                                                      aTCCTAAAAAGGCTGCATTCATAAAGAGTAAGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGACATTAAATTTTTGACCAAAAAAAATAAAACTCGTATCTTATTTCATCATAAAAGATTTCCGTTCAGAcata                                                                                 >  2:573156/1‑137 (MQ=255)
                                                        gcTAAAACGGCTGCCTTCCGAATGTGTGTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTaaa                                                                               <  2:1136474/139‑1 (MQ=255)
                                                                   tGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTg                                                                    >  1:563555/1‑140 (MQ=255)
                                                                    gCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGt                                                                   >  1:1390784/1‑140 (MQ=255)
                                                                     ccTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGtt                                                                  >  1:1450600/1‑140 (MQ=255)
                                                                                  tttGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                    <  1:1223777/140‑1 (MQ=255)
                                                                                  tttGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                    <  2:618755/140‑1 (MQ=255)
                                                                                  tttGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                    <  2:1326984/140‑1 (MQ=255)
                                                                                  tttGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                    <  2:450215/140‑1 (MQ=255)
                                                                                  tttGTGCATGAGATATCCCCCTTGAACATTGAATGAAGAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                    <  2:326376/140‑1 (MQ=255)
                                                                                  tgtgttCATGAGATATCCCCCTTGAACATGGAATGAATAGTCAGTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                    <  2:1357526/138‑1 (MQ=255)
                                                                                        cATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATCGCTAATTTGTTATTTTTTTGTATGAAGCtt                                              >  2:1706580/1‑140 (MQ=255)
                                                                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTCATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                      >  2:419960/1‑140 (MQ=255)
                                                                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGTAGCTCATAAAAGa                                      >  1:2421070/1‑140 (MQ=255)
                                                                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                      >  2:998865/1‑140 (MQ=255)
                                                                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                      >  2:2203555/1‑140 (MQ=255)
                                                                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                      >  2:1151935/1‑140 (MQ=255)
                                                                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                      >  2:1565589/1‑140 (MQ=255)
                                                                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCGTATAAAAGa                                      >  2:256985/1‑140 (MQ=255)
                                                                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGATATTTTTTTGTATGAAGCTTATAAAAGa                                      >  2:656733/1‑140 (MQ=255)
                                                                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGATAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                      >  2:151933/1‑140 (MQ=255)
                                                                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAAGAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                      >  2:1274220/1‑140 (MQ=255)
                                                                                                    cccTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAACAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACggg                                  <  2:892313/140‑1 (MQ=255)
                                                                                                    cccTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACggg                                  <  1:43521/140‑1 (MQ=255)
                                                                                                       ttGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGaaa                               >  2:184840/1‑140 (MQ=255)
                                                                                                       ttGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCaa                               >  2:20278/1‑140 (MQ=255)
                                                                                                       ttGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCaa                               >  2:2240833/1‑140 (MQ=255)
                                                                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAg                                                                            <  1:161192/95‑1 (MQ=255)
                                                                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAg                                                                            >  2:161192/1‑95 (MQ=255)
                                                                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTTTATGAAGATTATAAAAGACGGGCAAc                              >  1:2062759/1‑140 (MQ=255)
                                                                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAATCTTATAAAATACGGGCGAc                              >  2:1418954/1‑140 (MQ=255)
                                                                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAATACggcaacg                              >  2:1578110/1‑135 (MQ=255)
                                                                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAAc                              >  2:934484/1‑140 (MQ=255)
                                                                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAAc                              >  2:225271/1‑140 (MQ=255)
                                                                                                           aCATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTa                           <  2:2400326/140‑1 (MQ=255)
                                                                                                               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                  <  1:499511/129‑1 (MQ=255)
                                                                                                               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                  >  2:1771849/1‑129 (MQ=255)
                                                                                                               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                  <  1:1771849/129‑1 (MQ=255)
                                                                                                               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                  >  2:499511/1‑129 (MQ=255)
                                                                                                               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                  <  1:254429/129‑1 (MQ=255)
                                                                                                               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                  >  2:254429/1‑129 (MQ=255)
                                                                                                               tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                       >  1:2208306/1‑140 (MQ=255)
                                                                                                               tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                       >  1:2348239/1‑140 (MQ=255)
                                                                                                               tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTGTCATCTTAAAAGATATCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                       >  2:1431403/1‑140 (MQ=255)
                                                                                                                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                     >  1:1818382/1‑140 (MQ=255)
                                                                                                                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                     <  1:1179858/140‑1 (MQ=255)
                                                                                                                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                     <  1:1326975/140‑1 (MQ=255)
                                                                                                                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                     <  1:1352692/140‑1 (MQ=255)
                                                                                                                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                     <  1:682080/140‑1 (MQ=255)
                                                                                                                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                     <  1:329861/140‑1 (MQ=255)
                                                                                                                    gaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                  <  1:1227735/140‑1 (MQ=255)
                                                                                                                    gaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                  <  1:303383/140‑1 (MQ=255)
                                                                                                                    gaatACTCATTCATTGTTAGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTCTAAAAGACGGGCAACTTAAGCCCGGAg                  <  2:987005/140‑1 (MQ=255)
                                                                                                                    gaagcgTCCTTCATCTTTTGACCAAAAAAAATGACACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGTTCCGACTTCAATACCTACTTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                  <  2:1827505/135‑1 (MQ=255)
                                                                                                                      atAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTCTGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGt                <  1:950569/140‑1 (MQ=255)
                                                                                                                      atAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGt                <  2:2093840/140‑1 (MQ=255)
                                                                                                                        aGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGa              <  2:2016499/140‑1 (MQ=255)
                                                                                                                        aGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGa              <  2:265409/140‑1 (MQ=255)
                                                                                                                         gTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGaa             >  2:1856592/1‑140 (MQ=255)
                                                                                                                         gTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTAGGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGaa             <  2:65852/140‑1 (MQ=255)
                                                                                                                           cattcattTTTTGACCAAAAAAAATGAAACTCGTTTGTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg           >  2:1783199/1‑140 (MQ=255)
                                                                                                                           cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTGCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg           >  2:2315879/1‑140 (MQ=255)
                                                                                                                           cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTACTTTGTTATTTTTTTGTATGTAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg           >  2:2427286/1‑140 (MQ=255)
                                                                                                                           cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGTATCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg           >  2:1784506/1‑140 (MQ=255)
                                                                                                                           cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg           >  2:68099/1‑140 (MQ=255)
                                                                                                                           cattcattTTTTGACAAAAAAAAATGAAACTAGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAATCCCGGAGGTGAAgg           >  2:1581278/1‑140 (MQ=255)
                                                                                                                            attcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg           >  1:2214129/1‑140 (MQ=255)
                                                                                                                              tcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGaa        <  1:2377311/140‑1 (MQ=255)
                                                                                                                                    tttGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGACTGGTGc  >  1:2122890/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                       
CAGGAATTCCGATGTTAGCCAAATGCGCCGCAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGC  >  NZ_CP034943/3180428‑3180699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: