Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 480,215 T→A 5.5% P116P (CCT→CCA EO946_RS02465 → glycosyltransferase family 2 protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943480,2150TA 5.5% 715.5 / 21.7 217P116P (CCT→CCAEO946_RS02465glycosyltransferase family 2 protein
Reads supporting (aligned to +/- strand):  ref base T (63/140);  new base A (5/7);  total (69/148)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 217 total aligned reads displayed.

ATGGTCAACCTTTATTATCCGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGAC  >  NZ_CP034943/480078‑480353
                                                                                                                                         |                                                                                                                                          
aTGGTCAACCTTTATTATCCGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTaa                                                                                                                                          >  1:2174819/1‑140 (MQ=255)
aTGGTCAACCTTTATTATCCGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTaa                                                                                                                                          >  1:550734/1‑140 (MQ=255)
        ccagtattagCCGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTCTCAAAATGGTGATCACAAAACCGCCTAACGCCGGaa                                                                                                                                  <  1:1540496/136‑1 (MQ=255)
        ccTTTATTATCCGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGaa                                                                                                                                  <  2:859919/140‑1 (MQ=255)
        ccTTTATTATCCGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGaa                                                                                                                                  <  1:2285910/140‑1 (MQ=255)
           ttattaTCCGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAg                                                                                                                               >  2:594970/1‑140 (MQ=255)
           ttattaTCCGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGACAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAg                                                                                                                               >  2:209832/1‑140 (MQ=255)
             attatCCGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGc                                                                                                                             <  2:1533747/140‑1 (MQ=255)
               tatCCGCAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCaa                                                                                                                           <  2:844731/140‑1 (MQ=255)
               tatCCGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCaa                                                                                                                           <  2:613957/140‑1 (MQ=255)
                   cGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATcc                                                                                                                       >  2:2270102/1‑140 (MQ=255)
                   cGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATcc                                                                                                                       >  2:68605/1‑140 (MQ=255)
                   cGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATcc                                                                                                                       >  2:665030/1‑140 (MQ=255)
                    gAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCct                                                                                                                      <  1:405051/140‑1 (MQ=255)
                      aGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCctct                                                                                                                    >  1:1333191/1‑140 (MQ=255)
                         aCCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCa                                                                                                                 <  2:2148109/140‑1 (MQ=255)
                         aCCGTCTGGAGATTAGTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCa                                                                                                                 <  1:2038252/140‑1 (MQ=255)
                           cGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACt                                                                                                               <  2:278392/140‑1 (MQ=255)
                           cGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACt                                                                                                               <  2:2210632/140‑1 (MQ=255)
                           cGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACt                                                                                                               <  1:485885/140‑1 (MQ=255)
                            gTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTg                                                                                                              >  1:563903/1‑140 (MQ=255)
                                       attGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGAtt                                                                                                   >  1:136661/1‑140 (MQ=255)
                                                  cGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGa                                                                                        >  2:1256500/1‑140 (MQ=255)
                                                     tAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAATGGCCAATCCTCTGCACTGAACGCTGGATTTGCCGAATCGAACg                                                                                     >  2:1583271/1‑140 (MQ=255)
                                                     tAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACg                                                                                     >  2:1342597/1‑140 (MQ=255)
                                                     tAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACg                                                                                     <  2:1805445/140‑1 (MQ=255)
                                                     tAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACg                                                                                     <  2:1337376/140‑1 (MQ=255)
                                                     tAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACg                                                                                     >  2:36608/1‑140 (MQ=255)
                                                     tAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACg                                                                                     <  1:1406307/140‑1 (MQ=255)
                                                     tAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACg                                                                                     <  2:600572/140‑1 (MQ=255)
                                                     tAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACg                                                                                     <  2:782485/140‑1 (MQ=255)
                                                     tAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACg                                                                                     <  2:2041586/140‑1 (MQ=255)
                                                     tAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGAAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACg                                                                                     <  2:1227948/140‑1 (MQ=255)
                                                       aTTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGc                                                                                   <  1:2023600/140‑1 (MQ=255)
                                                          cttcAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGAt                                                                                <  2:1505747/140‑1 (MQ=255)
                                                             cAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGAtgtg                                                                             >  1:802206/1‑140 (MQ=255)
                                                               gACCGGACGTGTGATATAGTCAATGAATTGTCCGAGATATTCGTTTTTATCAAAATGGTGATCACAAAACCGCCTAACTCAGGAAATGGCAAATCCTCTGCACTGAACTTTGGATTTGCCGAATCGAACGGCGATGTGAt                                                                           <  1:209832/140‑1 (MQ=255)
                                                               gACCGGACGGGTGATATCGTCAATGAATTTTCCGATAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGAt                                                                           <  1:2223548/140‑1 (MQ=255)
                                                               gACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTCTCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGAt                                                                           <  2:263842/140‑1 (MQ=255)
                                                               gACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGAt                                                                           <  1:594970/140‑1 (MQ=255)
                                                               gACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGAt                                                                           <  2:1893868/140‑1 (MQ=255)
                                                               gACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGAt                                                                           <  1:68605/140‑1 (MQ=255)
                                                               gACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGAt                                                                           <  1:2270102/140‑1 (MQ=255)
                                                                aCCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATc                                                                          >  2:1617355/1‑140 (MQ=255)
                                                                aCCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATc                                                                          <  2:1389216/140‑1 (MQ=255)
                                                                 ccGGACGGGTGATATCGTCAATGACTGTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCt                                                                         <  2:307601/140‑1 (MQ=255)
                                                                 ccGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCt                                                                         <  2:514138/140‑1 (MQ=255)
                                                                     aCGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCtgt                                                                       >  2:549214/1‑138 (MQ=255)
                                                                          tGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATg                                                                <  2:446159/140‑1 (MQ=255)
                                                                          tGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATg                                                                <  2:2433577/140‑1 (MQ=255)
                                                                          tGATATCGTCAATGAATTTTCCGAGAAATACGATTTCATCAAAATGGTGATTACGAAGCCGCCAAATGCAGGAAAAGGAAAATCCTCTGCACTTAACTCGGGTTTTGCCGAATCGAACGGCGATGTGATCTGTGTGTATg                                                                >  2:337913/1‑140 (MQ=255)
                                                                          tGATATCGTCAATGAATTTTCCGAGAAATACGATTTCATCAAAATGGTGATTACGAAGCCGCCAAATGCAGGAAAAGGAAAATCCTCTGCACTTAACTCGGGTTTTGCCGAATCGAACGGCGATGTGATCTGTGTGTATg                                                                >  2:774784/1‑140 (MQ=255)
                                                                              atCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGc                                                            >  1:2088631/1‑140 (MQ=255)
                                                                              atCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGc                                                            >  1:1636737/1‑140 (MQ=255)
                                                                               tCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGAt                                                                           >  2:1143662/1‑124 (MQ=255)
                                                                               tCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGAt                                                                           <  1:1143662/124‑1 (MQ=255)
                                                                               tCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGcc                                                           <  1:606107/140‑1 (MQ=255)
                                                                               tCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGcc                                                           <  1:566044/140‑1 (MQ=255)
                                                                               tCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGcc                                                           >  1:376441/1‑140 (MQ=255)
                                                                                 gTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGa                                                         >  1:2013280/1‑140 (MQ=255)
                                                                                   cAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGAtgt                                                                              <  1:780111/117‑1 (MQ=255)
                                                                                   cAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGAtgt                                                                              >  2:164255/1‑117 (MQ=255)
                                                                                    aaTGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGacaa                                                      <  2:1168520/140‑1 (MQ=255)
                                                                                    aaTGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGacaa                                                      <  2:441544/140‑1 (MQ=255)
                                                                                    aaTGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGacaa                                                      <  2:938315/140‑1 (MQ=255)
                                                                                    aaTGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGacaa                                                      <  1:2212856/140‑1 (MQ=255)
                                                                                    aaTGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGacaa                                                      <  1:2409949/140‑1 (MQ=255)
                                                                                           tttCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGAt                                                                           <  1:956596/112‑1 (MQ=255)
                                                                                                  gacaTACGATTTTATCAAAATGGTGATCACAAAACCGCCGAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGCCGCCGACAACACCCCTGAGaaaa                                        <  2:2121749/137‑1 (MQ=255)
                                                                                                  gaAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATTTGATCTGTGTATATGACGCCGACAACACCCCTGAGaaaa                                        <  2:2408884/140‑1 (MQ=255)
                                                                                                  gaAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGaaaa                                        <  2:1990496/140‑1 (MQ=255)
                                                                                                     aTACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATgg                                     <  1:1256500/140‑1 (MQ=255)
                                                                                                     aTACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATgg                                     <  2:1124876/140‑1 (MQ=255)
                                                                                                     aTACGATTTCATCAAAATGGTGATTACGAAGCCGCCAAATGCAGGAAAAGGAAAATCCTCTGCACTTAACTCGGGTTTTGCCGAATCGAACGGCGATGTGATCTGTGTGTATGACGCCGACAACACGCCTGAGAAAATgg                                     <  2:1595697/140‑1 (MQ=255)
                                                                                                       aCGATTTTATCAAAATGGTGATCACAAAACCGCCTACCGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGcc                                   <  2:1926079/140‑1 (MQ=255)
                                                                                                       aCGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGcc                                   <  1:177184/140‑1 (MQ=255)
                                                                                                       aCGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGcc                                   <  1:1921552/140‑1 (MQ=255)
                                                                                                       aCGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGcc                                   <  2:2061970/140‑1 (MQ=255)
                                                                                                       aCGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGcc                                   <  2:2349688/140‑1 (MQ=255)
                                                                                                       aCGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAAGCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGcc                                   <  1:35457/140‑1 (MQ=255)
                                                                                                            tttATCAAAATGGTGATCACAAACCCGCCTAACGCCGGACAAGGCAAATCCTCTGCACTGAACTCTGGACTTGCCTAATCGAACGCCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTa                              <  1:2075568/140‑1 (MQ=255)
                                                                                                            tttATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTa                              <  1:1831337/140‑1 (MQ=255)
                                                                                                            tttATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTa                              <  1:764578/140‑1 (MQ=255)
                                                                                                            tttATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTa                              <  1:1479201/140‑1 (MQ=255)
                                                                                                             ttATCAAAATGGTGATCAAAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTAt                             <  1:244588/140‑1 (MQ=255)
                                                                                                              tATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTAtt                            <  1:390501/140‑1 (MQ=255)
                                                                                                              tATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTAtt                            <  1:2263929/140‑1 (MQ=255)
                                                                                                              tATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTAtt                            <  1:1016947/140‑1 (MQ=255)
                                                                                                                  aaaaTGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCt                        <  1:905327/140‑1 (MQ=255)
                                                                                                                        gTGATCACAAAACCGCCTACCGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCt                  <  2:573366/140‑1 (MQ=255)
                                                                                                                        gTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCt                  <  1:508559/140‑1 (MQ=255)
                                                                                                                        gTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCt                  <  1:301959/140‑1 (MQ=255)
                                                                                                                         tGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTc                 >  1:1054972/1‑140 (MQ=255)
                                                                                                                           aTCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATGTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCgg               >  1:2047897/1‑140 (MQ=255)
                                                                                                                           aTCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCgg               >  1:143106/1‑140 (MQ=255)
                                                                                                                                aaaaCCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCtga          >  2:173598/1‑140 (MQ=255)
                                                                                                                                    ccGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGatgaa      <  2:1054972/140‑1 (MQ=255)
                                                                                                                                    ccGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGatgaa      <  2:2165847/140‑1 (MQ=255)
                                                                                                                                       ccTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGatgaatga   >  2:1904856/1‑140 (MQ=255)
                                                                                                                                        cTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGAc  >  2:14609/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                          
ATGGTCAACCTTTATTATCCGAAGGACCGTCTGGAGATTATTGTGGTAAACGATAATTCTTCAGACCGGACGGGTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGAC  >  NZ_CP034943/480078‑480353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: