Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,180,581 T→C 6.4% intergenic (‑63/‑112) EO946_RS16450 ← / → EO946_RS16455 3‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,180,5810TC 6.4% 290.2 / 7.7 93intergenic (‑63/‑112)EO946_RS16450/EO946_RS164553‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein
Reads supporting (aligned to +/- strand):  ref base T (51/36);  new base C (3/3);  total (54/39)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

Only 100 of 101 total aligned reads displayed.

GCCGCAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTT  >  NZ_CP034943/3180454‑3180720
                                                                                                                                |                                                                                                                                            
gccgcAATCCCGGAACCCATTACCCCCGATCCTACAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCttt                                                                                                                                  <  2:681377/140‑1 (MQ=255)
gccgcAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCttt                                                                                                                                  <  2:957230/140‑1 (MQ=255)
gccgcAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCttt                                                                                                                                  <  2:933942/140‑1 (MQ=255)
gccgcAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCttt                                                                                                                                  <  2:681683/140‑1 (MQ=255)
gccgcAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCttt                                                                                                                                  <  2:361931/140‑1 (MQ=255)
  cgcAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCa                                                                                                                                >  1:2400326/1‑140 (MQ=255)
          cGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTaaaa                                                                                                                        <  1:287917/140‑1 (MQ=255)
            gAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGa                                                                                                                      >  2:1223777/1‑140 (MQ=255)
                   ttACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCgg                                                                                                               >  2:30701/1‑140 (MQ=255)
                   ttACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCgg                                                                                                               >  2:1577543/1‑140 (MQ=255)
                    tACCCCCGATCCTAAAACGGCTGCCGTCCGAATGTGTTTGTGCATGAGATAACCCCCTTGAACATTGAATGAATAGACATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGt                                                                                                              <  1:1952606/140‑1 (MQ=255)
                      cccccGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTcc                                                                                                            <  1:836158/140‑1 (MQ=255)
                        cccGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGa                                                                                                          <  1:1938842/140‑1 (MQ=255)
                         ccGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGAc                                                                                                         <  2:8039/140‑1 (MQ=255)
                          cGATCCTAAAACGGCTGCCTTCCGACTGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCAGGTTAAAAGATGTCCGGTCCGACt                                                                                                        >  2:2221037/1‑140 (MQ=255)
                          cGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACt                                                                                                        >  1:2093840/1‑140 (MQ=255)
                          cGAACCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTATTTCATCTTAAAATATGTCCGGTCCGACt                                                                                                        >  2:2039892/1‑140 (MQ=255)
                            aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCGCCTTGAACATTGAATGAATCGTCTTTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                                      >  2:2390610/1‑140 (MQ=255)
                            aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACtt                                                                                                       >  1:1624140/1‑140 (MQ=255)
                            aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                                      >  1:821493/1‑140 (MQ=255)
                            aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                                      >  1:798572/1‑140 (MQ=255)
                            aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                                      >  1:303157/1‑140 (MQ=255)
                            aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                                      >  2:2082806/1‑140 (MQ=255)
                            aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTAGACCAAAAAAAAAGAAAATCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                                      >  2:1738982/1‑140 (MQ=255)
                            aTCCTAAAAAGGCTGCATTCATAAAGAGTAAGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGACATTAAATTTTTGACCAAAAAAAATAAAACTCGTATCTTATTTCATCATAAAAGATTTCCGTTCAGAcata                                                                                                      >  2:573156/1‑137 (MQ=255)
                              gcTAAAACGGCTGCCTTCCGAATGTGTGTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTaaa                                                                                                    <  2:1136474/139‑1 (MQ=255)
                                         tGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTg                                                                                         >  1:563555/1‑140 (MQ=255)
                                          gCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGt                                                                                        >  1:1390784/1‑140 (MQ=255)
                                           ccTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGtt                                                                                       >  1:1450600/1‑140 (MQ=255)
                                                        tttGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                                         <  1:1223777/140‑1 (MQ=255)
                                                        tttGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                                         <  2:1326984/140‑1 (MQ=255)
                                                        tttGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                                         <  2:450215/140‑1 (MQ=255)
                                                        tttGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                                         <  2:618755/140‑1 (MQ=255)
                                                        tttGTGCATGAGATATCCCCCTTGAACATTGAATGAAGAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                                         <  2:326376/140‑1 (MQ=255)
                                                        tgtgttCATGAGATATCCCCCTTGAACATGGAATGAATAGTCAGTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                                         <  2:1357526/138‑1 (MQ=255)
                                                              cATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATCGCTAATTTGTTATTTTTTTGTATGAAGCtt                                                                   >  2:1706580/1‑140 (MQ=255)
                                                                      atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTCATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                           >  2:419960/1‑140 (MQ=255)
                                                                      atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGTAGCTCATAAAAGa                                                           >  1:2421070/1‑140 (MQ=255)
                                                                      atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                           >  2:2203555/1‑140 (MQ=255)
                                                                      atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                           >  2:1565589/1‑140 (MQ=255)
                                                                      atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                           >  2:1151935/1‑140 (MQ=255)
                                                                      atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCGTATAAAAGa                                                           >  2:256985/1‑140 (MQ=255)
                                                                      atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGATATTTTTTTGTATGAAGCTTATAAAAGa                                                           >  2:656733/1‑140 (MQ=255)
                                                                      atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGATAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                           >  2:151933/1‑140 (MQ=255)
                                                                      atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAAGAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                           >  2:1274220/1‑140 (MQ=255)
                                                                          cccTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAACAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACggg                                                       <  2:892313/140‑1 (MQ=255)
                                                                          cccTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACggg                                                       <  1:43521/140‑1 (MQ=255)
                                                                             ttGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGaaa                                                    >  2:184840/1‑140 (MQ=255)
                                                                             ttGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCaa                                                    >  2:2240833/1‑140 (MQ=255)
                                                                             ttGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCaa                                                    >  2:20278/1‑140 (MQ=255)
                                                                              tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAg                                                                                                 >  2:161192/1‑95 (MQ=255)
                                                                              tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAg                                                                                                 <  1:161192/95‑1 (MQ=255)
                                                                              tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTTTATGAAGATTATAAAAGACGGGCAAc                                                   >  1:2062759/1‑140 (MQ=255)
                                                                              tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAATCTTATAAAATACGGGCGAc                                                   >  2:1418954/1‑140 (MQ=255)
                                                                              tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAATACggcaacg                                                   >  2:1578110/1‑135 (MQ=255)
                                                                              tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAAc                                                   >  2:225271/1‑140 (MQ=255)
                                                                              tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAAc                                                   >  2:934484/1‑140 (MQ=255)
                                                                                 aCATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTa                                                <  2:2400326/140‑1 (MQ=255)
                                                                                     tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                       <  1:1771849/129‑1 (MQ=255)
                                                                                     tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                       >  2:254429/1‑129 (MQ=255)
                                                                                     tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                       >  2:499511/1‑129 (MQ=255)
                                                                                     tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                       <  1:254429/129‑1 (MQ=255)
                                                                                     tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                       <  1:499511/129‑1 (MQ=255)
                                                                                     tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                       >  2:1771849/1‑129 (MQ=255)
                                                                                     tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                                            >  1:2208306/1‑140 (MQ=255)
                                                                                     tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                                            >  1:2348239/1‑140 (MQ=255)
                                                                                     tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTGTCATCTTAAAAGATATCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                                            >  2:1431403/1‑140 (MQ=255)
                                                                                       aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                          <  1:1179858/140‑1 (MQ=255)
                                                                                       aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                          <  1:329861/140‑1 (MQ=255)
                                                                                       aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                          <  1:1326975/140‑1 (MQ=255)
                                                                                       aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                          <  1:1352692/140‑1 (MQ=255)
                                                                                       aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                          <  1:682080/140‑1 (MQ=255)
                                                                                       aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                          >  1:1818382/1‑140 (MQ=255)
                                                                                          gaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                                       <  1:1227735/140‑1 (MQ=255)
                                                                                          gaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                                       <  1:303383/140‑1 (MQ=255)
                                                                                          gaatACTCATTCATTGTTAGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTCTAAAAGACGGGCAACTTAAGCCCGGAg                                       <  2:987005/140‑1 (MQ=255)
                                                                                          gaagcgTCCTTCATCTTTTGACCAAAAAAAATGACACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGTTCCGACTTCAATACCTACTTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                                       <  2:1827505/135‑1 (MQ=255)
                                                                                            atAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTCTGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGt                                     <  1:950569/140‑1 (MQ=255)
                                                                                            atAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGt                                     <  2:2093840/140‑1 (MQ=255)
                                                                                              aGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGa                                   <  2:265409/140‑1 (MQ=255)
                                                                                              aGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGa                                   <  2:2016499/140‑1 (MQ=255)
                                                                                               gTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGaa                                  >  2:1856592/1‑140 (MQ=255)
                                                                                               gTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTAGGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGaa                                  <  2:65852/140‑1 (MQ=255)
                                                                                                 cattcattTTTTGACCAAAAAAAATGAAACTCGTTTGTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                >  2:1783199/1‑140 (MQ=255)
                                                                                                 cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTGCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                >  2:2315879/1‑140 (MQ=255)
                                                                                                 cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTACTTTGTTATTTTTTTGTATGTAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                >  2:2427286/1‑140 (MQ=255)
                                                                                                 cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGTATCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                >  2:1784506/1‑140 (MQ=255)
                                                                                                 cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                >  2:68099/1‑140 (MQ=255)
                                                                                                 cattcattTTTTGACAAAAAAAAATGAAACTAGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAATCCCGGAGGTGAAgg                                >  2:1581278/1‑140 (MQ=255)
                                                                                                  attcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                >  1:2214129/1‑140 (MQ=255)
                                                                                                    tcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGaa                             <  1:2377311/140‑1 (MQ=255)
                                                                                                          tttGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGACTGGTGc                       >  1:2122890/1‑140 (MQ=255)
                                                                                                                                tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt  <  1:428793/140‑1 (MQ=255)
                                                                                                                                tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt  <  1:877626/140‑1 (MQ=255)
                                                                                                                                tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt  <  1:918684/140‑1 (MQ=255)
                                                                                                                                tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt  <  2:1390784/140‑1 (MQ=255)
                                                                                                                                tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt  <  1:966299/140‑1 (MQ=255)
                                                                                                                                tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGGCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt  <  1:995584/140‑1 (MQ=255)
                                                                                                                                tCGTTTCTTCTTTCATCTTAAAAGAGTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt  <  2:972963/140‑1 (MQ=255)
                                                                                                                                tCGTTTCTTCTTTCATCTAAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt  <  1:1577283/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                            
GCCGCAATCCCGGAACCCATTACCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTT  >  NZ_CP034943/3180454‑3180720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: