Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,180,614 C→T 5.4% intergenic (‑96/‑79) EO946_RS16450 ← / → EO946_RS16455 3‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,180,6140CT 5.4% 355.4 / 6.9 114intergenic (‑96/‑79)EO946_RS16450/EO946_RS164553‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein
Reads supporting (aligned to +/- strand):  ref base C (50/57);  new base T (3/3);  total (54/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01

Only 100 of 115 total aligned reads displayed.

CCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGG  >  NZ_CP034943/3180476‑3180751
                                                                                                                                           |                                                                                                                                          
cccccGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTcc                                                                                                                                           <  1:836158/140‑1 (MQ=255)
  cccGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGa                                                                                                                                         <  1:1938842/140‑1 (MQ=255)
   ccGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGAc                                                                                                                                        <  2:8039/140‑1 (MQ=255)
    cGATCCTAAAACGGCTGCCTTCCGACTGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCAGGTTAAAAGATGTCCGGTCCGACt                                                                                                                                       >  2:2221037/1‑140 (MQ=255)
    cGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACt                                                                                                                                       >  1:2093840/1‑140 (MQ=255)
    cGAACCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTATTTCATCTTAAAATATGTCCGGTCCGACt                                                                                                                                       >  2:2039892/1‑140 (MQ=255)
      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCGCCTTGAACATTGAATGAATCGTCTTTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                                                                     >  2:2390610/1‑140 (MQ=255)
      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACtt                                                                                                                                      >  1:1624140/1‑140 (MQ=255)
      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                                                                     >  1:821493/1‑140 (MQ=255)
      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                                                                     >  1:798572/1‑140 (MQ=255)
      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                                                                     >  2:2082806/1‑140 (MQ=255)
      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                                                                     >  1:303157/1‑140 (MQ=255)
      aTCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTAGACCAAAAAAAAAGAAAATCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTc                                                                                                                                     >  2:1738982/1‑140 (MQ=255)
        gcTAAAACGGCTGCCTTCCGAATGTGTGTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTaaa                                                                                                                                   <  2:1136474/139‑1 (MQ=255)
                   tGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTg                                                                                                                        >  1:563555/1‑140 (MQ=255)
                     ccTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGtt                                                                                                                      >  1:1450600/1‑140 (MQ=255)
                                  tttGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                                                                        <  2:1326984/140‑1 (MQ=255)
                                  tttGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                                                                        <  2:450215/140‑1 (MQ=255)
                                  tttGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                                                                        <  2:618755/140‑1 (MQ=255)
                                  tttGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                                                                        <  1:1223777/140‑1 (MQ=255)
                                  tttGTGCATGAGATATCCCCCTTGAACATTGAATGAAGAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                                                                        <  2:326376/140‑1 (MQ=255)
                                  tgtgttCATGAGATATCCCCCTTGAACATGGAATGAATAGTCAGTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATg                                                                                                        <  2:1357526/138‑1 (MQ=255)
                                        cATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATCGCTAATTTGTTATTTTTTTGTATGAAGCtt                                                                                                  >  2:1706580/1‑140 (MQ=255)
                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTCATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                          >  2:419960/1‑140 (MQ=255)
                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGTAGCTCATAAAAGa                                                                                          >  1:2421070/1‑140 (MQ=255)
                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                          >  2:1151935/1‑140 (MQ=255)
                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                          >  2:1565589/1‑140 (MQ=255)
                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                          >  2:2203555/1‑140 (MQ=255)
                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGATATTTTTTTGTATGAAGCTTATAAAAGa                                                                                          >  2:656733/1‑140 (MQ=255)
                                                atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGATAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                          >  2:151933/1‑140 (MQ=255)
                                                    cccTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAACAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACggg                                                                                      <  2:892313/140‑1 (MQ=255)
                                                    cccTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACggg                                                                                      <  1:43521/140‑1 (MQ=255)
                                                       ttGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGaaa                                                                                   >  2:184840/1‑140 (MQ=255)
                                                       ttGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCaa                                                                                   >  2:2240833/1‑140 (MQ=255)
                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAg                                                                                                                                >  2:161192/1‑95 (MQ=255)
                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAg                                                                                                                                <  1:161192/95‑1 (MQ=255)
                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTTTATGAAGATTATAAAAGACGGGCAAc                                                                                  >  1:2062759/1‑140 (MQ=255)
                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAATCTTATAAAATACGGGCGAc                                                                                  >  2:1418954/1‑140 (MQ=255)
                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAATACggcaacg                                                                                  >  2:1578110/1‑135 (MQ=255)
                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAAc                                                                                  >  2:934484/1‑140 (MQ=255)
                                                        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAAc                                                                                  >  2:225271/1‑140 (MQ=255)
                                                           aCATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTa                                                                               <  2:2400326/140‑1 (MQ=255)
                                                               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                      <  1:499511/129‑1 (MQ=255)
                                                               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                      <  1:254429/129‑1 (MQ=255)
                                                               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                      >  2:254429/1‑129 (MQ=255)
                                                               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                      <  1:1771849/129‑1 (MQ=255)
                                                               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                      >  2:499511/1‑129 (MQ=255)
                                                               tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                                                                           >  1:2348239/1‑140 (MQ=255)
                                                               tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                                                                           >  1:2208306/1‑140 (MQ=255)
                                                               tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTGTCATCTTAAAAGATATCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                                                                           >  2:1431403/1‑140 (MQ=255)
                                                                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                         <  1:1179858/140‑1 (MQ=255)
                                                                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                         <  1:329861/140‑1 (MQ=255)
                                                                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                         <  1:682080/140‑1 (MQ=255)
                                                                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                         <  1:1326975/140‑1 (MQ=255)
                                                                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                         <  1:1352692/140‑1 (MQ=255)
                                                                    gaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                                                                      <  1:1227735/140‑1 (MQ=255)
                                                                    gaatACTCATTCATTGTTAGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTCTAAAAGACGGGCAACTTAAGCCCGGAg                                                                      <  2:987005/140‑1 (MQ=255)
                                                                    gaagcgTCCTTCATCTTTTGACCAAAAAAAATGACACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGTTCCGACTTCAATACCTACTTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                                                                      <  2:1827505/135‑1 (MQ=255)
                                                                      atAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTCTGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGt                                                                    <  1:950569/140‑1 (MQ=255)
                                                                      atAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGt                                                                    <  2:2093840/140‑1 (MQ=255)
                                                                        aGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGa                                                                  <  2:265409/140‑1 (MQ=255)
                                                                        aGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGa                                                                  <  2:2016499/140‑1 (MQ=255)
                                                                         gTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGaa                                                                 >  2:1856592/1‑140 (MQ=255)
                                                                         gTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTAGGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGaa                                                                 <  2:65852/140‑1 (MQ=255)
                                                                           cattcattTTTTGACCAAAAAAAATGAAACTCGTTTGTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                               >  2:1783199/1‑140 (MQ=255)
                                                                           cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTACTTTGTTATTTTTTTGTATGTAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                               >  2:2427286/1‑140 (MQ=255)
                                                                           cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGTATCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                               >  2:1784506/1‑140 (MQ=255)
                                                                           cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                               >  2:68099/1‑140 (MQ=255)
                                                                           cattcattTTTTGACAAAAAAAAATGAAACTAGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAATCCCGGAGGTGAAgg                                                               >  2:1581278/1‑140 (MQ=255)
                                                                            attcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                               >  1:2214129/1‑140 (MQ=255)
                                                                              tcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGaa                                                            <  1:2377311/140‑1 (MQ=255)
                                                                                    tttGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGACTGGTGc                                                      >  1:2122890/1‑140 (MQ=255)
                                                                                                          tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                 <  1:428793/140‑1 (MQ=255)
                                                                                                          tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                 <  2:1390784/140‑1 (MQ=255)
                                                                                                          tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                 <  1:966299/140‑1 (MQ=255)
                                                                                                          tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                 <  1:877626/140‑1 (MQ=255)
                                                                                                          tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGGCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                 <  1:995584/140‑1 (MQ=255)
                                                                                                          tCGTTTCTTCTTTCATCTTAAAAGAGTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                 <  2:972963/140‑1 (MQ=255)
                                                                                                          tCGTTTCTTCTTTCATCTAAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                 <  1:1577283/140‑1 (MQ=255)
                                                                                                               tcttctTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAgcg                            <  1:1097894/140‑1 (MQ=255)
                                                                                                               tcttctTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAgcg                            <  2:1527261/140‑1 (MQ=255)
                                                                                                               tcttctTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAgcg                            <  2:563604/140‑1 (MQ=255)
                                                                                                               tcttctTTCATCTTAAAAGATTTCCGGGCCGACTTCAATAGCTAATTGGTTATTTTTTTGTATGCAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAgcg                            <  2:588693/140‑1 (MQ=255)
                                                                                                                 ttcttTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCa                          >  2:1209649/1‑140 (MQ=255)
                                                                                                                 ttcttTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCa                          >  2:334914/1‑140 (MQ=255)
                                                                                                                      tCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCa                     >  1:1727712/1‑140 (MQ=255)
                                                                                                                             aaacGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTATTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggc              <  1:1872235/140‑1 (MQ=255)
                                                                                                                             aaaaGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggc              <  2:1779905/140‑1 (MQ=255)
                                                                                                                              aaaGATTTCCTGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggcg             <  1:2036008/140‑1 (MQ=255)
                                                                                                                              aaaGATTTCCGGTCCGACTTCAATAGCTAATTTGTGAGTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggcg             <  2:1004196/140‑1 (MQ=255)
                                                                                                                                    ttCCGGTCCGACTTCAATAGCTAATTTTTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAACAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa       <  2:598117/140‑1 (MQ=255)
                                                                                                                                    ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAATACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa       >  1:690749/1‑140 (MQ=255)
                                                                                                                                    ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa       <  2:905495/140‑1 (MQ=255)
                                                                                                                                    ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa       <  1:1577543/140‑1 (MQ=255)
                                                                                                                                    ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa       <  1:30701/140‑1 (MQ=255)
                                                                                                                                    ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGACTTCAGCGCAAGTCAGCGCGGCGAGCGTa       <  2:881008/140‑1 (MQ=255)
                                                                                                                                    ttCCGGTCCGACTTCAATAGCTAATTGGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa       <  2:249281/140‑1 (MQ=255)
                                                                                                                                     tCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCCGCGCGGCGAGCGTaa      <  1:1209649/140‑1 (MQ=255)
                                                                                                                                     tCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTaa      <  1:334914/140‑1 (MQ=255)
                                                                                                                                         gTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAggg  <  2:2348239/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CCCCCGATCCTAAAACGGCTGCCTTCCGAATGTGTTTGTGCATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGG  >  NZ_CP034943/3180476‑3180751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: