Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,180,654 T→C 5.8% intergenic (‑136/‑39) EO946_RS16450 ← / → EO946_RS16455 3‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,180,6540TC 5.8% 328.8 / 7.2 104intergenic (‑136/‑39)EO946_RS16450/EO946_RS164553‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein
Reads supporting (aligned to +/- strand):  ref base T (41/56);  new base C (3/3);  total (45/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 106 total aligned reads displayed.

CATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTACGGCGGCGGAAAACGGAC  >  NZ_CP034943/3180516‑3180793
                                                                                                                                           |                                                                                                                                           
cATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATCGCTAATTTGTTATTTTTTTGTATGAAGCtt                                                                                                                                            >  2:1706580/1‑140 (MQ=255)
        atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTCATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                    >  2:419960/1‑140 (MQ=255)
        atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGTAGCTCATAAAAGa                                                                                                                                    >  1:2421070/1‑140 (MQ=255)
        atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                    >  2:1151935/1‑140 (MQ=255)
        atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                    >  2:1565589/1‑140 (MQ=255)
        atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                    >  2:2203555/1‑140 (MQ=255)
        atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCGTATAAAAGa                                                                                                                                    >  2:256985/1‑140 (MQ=255)
        atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGATATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                    >  2:656733/1‑140 (MQ=255)
        atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGATAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                    >  2:151933/1‑140 (MQ=255)
        atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAAGAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                    >  2:1274220/1‑140 (MQ=255)
            cccTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAACAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACggg                                                                                                                                <  2:892313/140‑1 (MQ=255)
            cccTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACggg                                                                                                                                <  1:43521/140‑1 (MQ=255)
               ttGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGaaa                                                                                                                             >  2:184840/1‑140 (MQ=255)
               ttGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCaa                                                                                                                             >  2:20278/1‑140 (MQ=255)
               ttGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCaa                                                                                                                             >  2:2240833/1‑140 (MQ=255)
                tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAATCTTATAAAATACGGGCGAc                                                                                                                            >  2:1418954/1‑140 (MQ=255)
                tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAATACggcaacg                                                                                                                            >  2:1578110/1‑135 (MQ=255)
                tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAAc                                                                                                                            >  2:225271/1‑140 (MQ=255)
                tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAAc                                                                                                                            >  2:934484/1‑140 (MQ=255)
                   aCATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTa                                                                                                                         <  2:2400326/140‑1 (MQ=255)
                       tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                                                                <  1:1771849/129‑1 (MQ=255)
                       tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                                                                >  2:1771849/1‑129 (MQ=255)
                       tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                                                                >  2:254429/1‑129 (MQ=255)
                       tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                                                                <  1:254429/129‑1 (MQ=255)
                       tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                                                                >  2:499511/1‑129 (MQ=255)
                       tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                                                                <  1:499511/129‑1 (MQ=255)
                       tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                                                                                                                     >  1:2208306/1‑140 (MQ=255)
                       tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                                                                                                                     >  1:2348239/1‑140 (MQ=255)
                       tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTGTCATCTTAAAAGATATCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                                                                                                                     >  2:1431403/1‑140 (MQ=255)
                         aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                                                                   >  1:1818382/1‑140 (MQ=255)
                         aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                                                                   <  1:1179858/140‑1 (MQ=255)
                         aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                                                                   <  1:1326975/140‑1 (MQ=255)
                         aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                                                                   <  1:329861/140‑1 (MQ=255)
                         aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                                                                   <  1:1352692/140‑1 (MQ=255)
                            gaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                                                                                                                <  1:1227735/140‑1 (MQ=255)
                            gaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                                                                                                                <  1:303383/140‑1 (MQ=255)
                            gaatACTCATTCATTGTTAGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTCTAAAAGACGGGCAACTTAAGCCCGGAg                                                                                                                <  2:987005/140‑1 (MQ=255)
                            gaagcgTCCTTCATCTTTTGACCAAAAAAAATGACACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGTTCCGACTTCAATACCTACTTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                                                                                                                <  2:1827505/135‑1 (MQ=255)
                              atAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTCTGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGt                                                                                                              <  1:950569/140‑1 (MQ=255)
                              atAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGt                                                                                                              <  2:2093840/140‑1 (MQ=255)
                                aGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGa                                                                                                            <  2:2016499/140‑1 (MQ=255)
                                aGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGa                                                                                                            <  2:265409/140‑1 (MQ=255)
                                 gTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGaa                                                                                                           >  2:1856592/1‑140 (MQ=255)
                                 gTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTAGGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGaa                                                                                                           <  2:65852/140‑1 (MQ=255)
                                   cattcattTTTTGACCAAAAAAAATGAAACTCGTTTGTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                                                                         >  2:1783199/1‑140 (MQ=255)
                                   cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTGCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                                                                         >  2:2315879/1‑140 (MQ=255)
                                   cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTACTTTGTTATTTTTTTGTATGTAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                                                                         >  2:2427286/1‑140 (MQ=255)
                                   cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGTATCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                                                                         >  2:1784506/1‑140 (MQ=255)
                                   cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                                                                         >  2:68099/1‑140 (MQ=255)
                                   cattcattTTTTGACAAAAAAAAATGAAACTAGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAATCCCGGAGGTGAAgg                                                                                                         >  2:1581278/1‑140 (MQ=255)
                                    attcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                                                                         >  1:2214129/1‑140 (MQ=255)
                                      tcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGaa                                                                                                      <  1:2377311/140‑1 (MQ=255)
                                            tttGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGACTGGTGc                                                                                                >  1:2122890/1‑140 (MQ=255)
                                                                 tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                           <  1:428793/140‑1 (MQ=255)
                                                                 tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                           <  2:1390784/140‑1 (MQ=255)
                                                                 tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                           <  1:877626/140‑1 (MQ=255)
                                                                 tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                           <  1:918684/140‑1 (MQ=255)
                                                                 tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                           <  1:966299/140‑1 (MQ=255)
                                                                 tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGGCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                           <  1:995584/140‑1 (MQ=255)
                                                                 tCGTTTCTTCTTTCATCTTAAAAGAGTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                           <  2:972963/140‑1 (MQ=255)
                                                                 tCGTTTCTTCTTTCATCTAAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                           <  1:1577283/140‑1 (MQ=255)
                                                                      tcttctTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAgcg                                                                      <  1:1097894/140‑1 (MQ=255)
                                                                      tcttctTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAgcg                                                                      <  2:1527261/140‑1 (MQ=255)
                                                                      tcttctTTCATCTTAAAAGATTTCCGGGCCGACTTCAATAGCTAATTGGTTATTTTTTTGTATGCAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAgcg                                                                      <  2:588693/140‑1 (MQ=255)
                                                                        ttcttTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCa                                                                    >  2:1209649/1‑140 (MQ=255)
                                                                        ttcttTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCa                                                                    >  2:334914/1‑140 (MQ=255)
                                                                             tCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCa                                                               >  1:1727712/1‑140 (MQ=255)
                                                                                    aaacGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTATTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggc                                                        <  1:1872235/140‑1 (MQ=255)
                                                                                    aaaaGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggc                                                        <  2:1779905/140‑1 (MQ=255)
                                                                                     acaGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTGCAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggcg                                                       <  1:616255/138‑1 (MQ=255)
                                                                                     aaaGATTTCCTGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggcg                                                       <  1:2036008/140‑1 (MQ=255)
                                                                                     aaaGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggcg                                                       <  1:2149972/140‑1 (MQ=255)
                                                                                     aaaGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggcg                                                       <  2:880676/140‑1 (MQ=255)
                                                                                     aaaGATTTCCGGTCCGACTTCAATAGCTAATTTGTGAGTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggcg                                                       <  2:1004196/140‑1 (MQ=255)
                                                                                           ttCCGGTCCGACTTCAATAGCTAATTTTTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAACAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa                                                 <  2:598117/140‑1 (MQ=255)
                                                                                           ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAATACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa                                                 >  1:690749/1‑140 (MQ=255)
                                                                                           ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa                                                 <  2:905495/140‑1 (MQ=255)
                                                                                           ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa                                                 <  1:1577543/140‑1 (MQ=255)
                                                                                           ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa                                                 <  1:30701/140‑1 (MQ=255)
                                                                                           ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGACTTCAGCGCAAGTCAGCGCGGCGAGCGTa                                                 <  2:881008/140‑1 (MQ=255)
                                                                                           ttCCGGTCCGACTTCAATAGCTAATTGGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa                                                 <  2:249281/140‑1 (MQ=255)
                                                                                            tCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCCGCGCGGCGAGCGTaa                                                <  1:1209649/140‑1 (MQ=255)
                                                                                            tCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTaa                                                <  1:334914/140‑1 (MQ=255)
                                                                                                gTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAggg                                            <  2:2348239/140‑1 (MQ=255)
                                                                                                      cTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGc                                      <  1:1126021/140‑1 (MQ=255)
                                                                                                      cTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGc                                      <  2:724604/140‑1 (MQ=255)
                                                                                                      cTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAACCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGc                                      <  1:1588317/140‑1 (MQ=255)
                                                                                                      cTTCAATAGCTAATTTGTTATTTTTTTGTAAGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGc                                      <  1:274681/140‑1 (MQ=255)
                                                                                                               cTAATTTGTTATTTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGa                              <  2:2214129/140‑1 (MQ=255)
                                                                                                               cTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCTGCGAGCGTAAATGGAGATGCCGGTCCGAc                             >  2:2422682/1‑140 (MQ=255)
                                                                                                               cTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGAc                             <  2:798572/140‑1 (MQ=255)
                                                                                                               cTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGAc                             >  2:119527/1‑140 (MQ=255)
                                                                                                               cTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGAc                             <  2:1624140/140‑1 (MQ=255)
                                                                                                               cTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGAc                             <  2:821493/140‑1 (MQ=255)
                                                                                                               cTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGAc                             >  2:316515/1‑140 (MQ=255)
                                                                                                                tAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCCACTTAAGCCCGGCGGTGAAGGGAATGGTGCGTGAGAAAAAATATCCGTCTTCAGCGCACGTCAGCGCGGCGGGCGTCAAGGGAGCTGCCGGGCCgccg                            >  1:1348080/1‑137 (MQ=255)
                                                                                                                tAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTCAAGGGAGATGCCGGTCCGACt                            >  1:1348109/1‑140 (MQ=255)
                                                                                                                tAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACt                            <  2:563555/140‑1 (MQ=255)
                                                                                                                         aTTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTAc                   >  2:8648/1‑140 (MQ=255)
                                                                                                                         aTTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTAc                   >  2:157026/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CATGAGATATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTACGGCGGCGGAAAACGGAC  >  NZ_CP034943/3180516‑3180793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: