Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,180,663 A→T 6.0% intergenic (‑145/‑30) EO946_RS16450 ← / → EO946_RS16455 3‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,180,6630AT 6.0% 326.0 / 10.3 100intergenic (‑145/‑30)EO946_RS16450/EO946_RS164553‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein
Reads supporting (aligned to +/- strand):  ref base A (34/60);  new base T (3/3);  total (37/63)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 120 total aligned reads displayed.

ATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTACGGCGGCGGAAAACGGACAAGTCAAAA  >  NZ_CP034943/3180524‑3180802
                                                                                                                                            |                                                                                                                                           
atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTCATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                             >  2:419960/1‑140 (MQ=255)
atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGTAGCTCATAAAAGa                                                                                                                                             >  1:2421070/1‑140 (MQ=255)
atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                             >  2:1151935/1‑140 (MQ=255)
atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                             >  2:1565589/1‑140 (MQ=255)
atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                             >  2:2203555/1‑140 (MQ=255)
atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGATATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                             >  2:656733/1‑140 (MQ=255)
atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGATAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                             >  2:151933/1‑140 (MQ=255)
atCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAAGAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGa                                                                                                                                             >  2:1274220/1‑140 (MQ=255)
    cccTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAACAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACggg                                                                                                                                         <  2:892313/140‑1 (MQ=255)
    cccTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACggg                                                                                                                                         <  1:43521/140‑1 (MQ=255)
       ttGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCaa                                                                                                                                      >  2:2240833/1‑140 (MQ=255)
       ttGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCaa                                                                                                                                      >  2:20278/1‑140 (MQ=255)
        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAATCTTATAAAATACGGGCGAc                                                                                                                                     >  2:1418954/1‑140 (MQ=255)
        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAATACggcaacg                                                                                                                                     >  2:1578110/1‑135 (MQ=255)
        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAAc                                                                                                                                     >  2:934484/1‑140 (MQ=255)
        tGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAAc                                                                                                                                     >  2:225271/1‑140 (MQ=255)
           aCATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTa                                                                                                                                  <  2:2400326/140‑1 (MQ=255)
               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                                                                         <  1:1771849/129‑1 (MQ=255)
               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                                                                         <  1:499511/129‑1 (MQ=255)
               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                                                                         >  2:254429/1‑129 (MQ=255)
               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                                                                         <  1:254429/129‑1 (MQ=255)
               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                                                                         >  2:499511/1‑129 (MQ=255)
               tgaatgaaTAGTCATTCATTTTTTGACTAAAAAAAATGAAACCCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTTCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCCTATAAAAGTCggg                                                                                                                                         >  2:1771849/1‑129 (MQ=255)
               tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                                                                                                                              >  1:2348239/1‑140 (MQ=255)
               tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                                                                                                                              >  1:2208306/1‑140 (MQ=255)
               tgaatgaaTAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTGTCATCTTAAAAGATATCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGcc                                                                                                                              >  2:1431403/1‑140 (MQ=255)
                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                                                                            <  1:1352692/140‑1 (MQ=255)
                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                                                                            <  1:1179858/140‑1 (MQ=255)
                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                                                                            <  1:682080/140‑1 (MQ=255)
                 aatgaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCg                                                                                                                            >  1:1818382/1‑140 (MQ=255)
                    gaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                                                                                                                         <  1:1227735/140‑1 (MQ=255)
                    gaatAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                                                                                                                         <  1:303383/140‑1 (MQ=255)
                    gaatACTCATTCATTGTTAGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTCTAAAAGACGGGCAACTTAAGCCCGGAg                                                                                                                         <  2:987005/140‑1 (MQ=255)
                    gaagcgTCCTTCATCTTTTGACCAAAAAAAATGACACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGTTCCGACTTCAATACCTACTTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAg                                                                                                                         <  2:1827505/135‑1 (MQ=255)
                      atAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTCTGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGt                                                                                                                       <  1:950569/140‑1 (MQ=255)
                      atAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGt                                                                                                                       <  2:2093840/140‑1 (MQ=255)
                        aGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGa                                                                                                                     <  2:265409/140‑1 (MQ=255)
                        aGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGa                                                                                                                     <  2:2016499/140‑1 (MQ=255)
                         gTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGaa                                                                                                                    >  2:1856592/1‑140 (MQ=255)
                         gTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTAGGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGaa                                                                                                                    <  2:65852/140‑1 (MQ=255)
                           cattcattTTTTGACCAAAAAAAATGAAACTCGTTTGTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                                                                                  >  2:1783199/1‑140 (MQ=255)
                           cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTGCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                                                                                  >  2:2315879/1‑140 (MQ=255)
                           cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTACTTTGTTATTTTTTTGTATGTAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                                                                                  >  2:2427286/1‑140 (MQ=255)
                           cattcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGTATCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                                                                                  >  2:1784506/1‑140 (MQ=255)
                           cattcattTTTTGACAAAAAAAAATGAAACTAGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAATCCCGGAGGTGAAgg                                                                                                                  >  2:1581278/1‑140 (MQ=255)
                            attcattTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAgg                                                                                                                  >  1:2214129/1‑140 (MQ=255)
                                    tttGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGACTGGTGc                                                                                                         >  1:2122890/1‑140 (MQ=255)
                                                         tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                                    <  1:966299/140‑1 (MQ=255)
                                                         tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                                    <  1:918684/140‑1 (MQ=255)
                                                         tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                                    <  1:428793/140‑1 (MQ=255)
                                                         tCGTTTCTTCTTTCATCTTAAAAGATTTCCGGGCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                                    <  1:995584/140‑1 (MQ=255)
                                                         tCGTTTCTTCTTTCATCTTAAAAGAGTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                                    <  2:972963/140‑1 (MQ=255)
                                                         tCGTTTCTTCTTTCATCTAAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCtt                                                                                    <  1:1577283/140‑1 (MQ=255)
                                                              tcttctTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAgcg                                                                               <  2:563604/140‑1 (MQ=255)
                                                              tcttctTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAgcg                                                                               <  1:1097894/140‑1 (MQ=255)
                                                              tcttctTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAgcg                                                                               <  2:1527261/140‑1 (MQ=255)
                                                              tcttctTTCATCTTAAAAGATTTCCGGGCCGACTTCAATAGCTAATTGGTTATTTTTTTGTATGCAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAgcg                                                                               <  2:588693/140‑1 (MQ=255)
                                                                ttcttTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCa                                                                             >  2:334914/1‑140 (MQ=255)
                                                                ttcttTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCa                                                                             >  2:1209649/1‑140 (MQ=255)
                                                                            aaacGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTATTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggc                                                                 <  1:1872235/140‑1 (MQ=255)
                                                                            aaaaGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggc                                                                 <  2:1779905/140‑1 (MQ=255)
                                                                             acaGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTGCAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggcg                                                                <  1:616255/138‑1 (MQ=255)
                                                                             aaaGATTTCCTGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggcg                                                                <  1:2036008/140‑1 (MQ=255)
                                                                             aaaGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggcg                                                                <  1:2149972/140‑1 (MQ=255)
                                                                             aaaGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggcg                                                                <  2:880676/140‑1 (MQ=255)
                                                                             aaaGATTTCCGGTCCGACTTCAATAGCTAATTTGTGAGTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCgcggcg                                                                <  2:1004196/140‑1 (MQ=255)
                                                                                   ttCCGGTCCGACTTCAATAGCTAATTTTTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAACAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa                                                          <  2:598117/140‑1 (MQ=255)
                                                                                   ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAATACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa                                                          >  1:690749/1‑140 (MQ=255)
                                                                                   ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa                                                          <  1:1577543/140‑1 (MQ=255)
                                                                                   ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa                                                          <  2:905495/140‑1 (MQ=255)
                                                                                   ttCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa                                                          <  1:30701/140‑1 (MQ=255)
                                                                                   ttCCGGTCCGACTTCAATAGCTAATTGGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTa                                                          <  2:249281/140‑1 (MQ=255)
                                                                                    tCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCCGCGCGGCGAGCGTaa                                                         <  1:1209649/140‑1 (MQ=255)
                                                                                    tCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTaa                                                         <  1:334914/140‑1 (MQ=255)
                                                                                        gTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAggg                                                     <  2:2348239/140‑1 (MQ=255)
                                                                                              cTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGc                                               <  1:1126021/140‑1 (MQ=255)
                                                                                              cTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGc                                               <  2:724604/140‑1 (MQ=255)
                                                                                              cTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAACCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGc                                               <  1:1588317/140‑1 (MQ=255)
                                                                                              cTTCAATAGCTAATTTGTTATTTTTTTGTAAGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGc                                               <  1:274681/140‑1 (MQ=255)
                                                                                                       cTAATTTGTTATTTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGa                                       <  2:2214129/140‑1 (MQ=255)
                                                                                                       cTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCTGCGAGCGTAAATGGAGATGCCGGTCCGAc                                      >  2:2422682/1‑140 (MQ=255)
                                                                                                       cTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGAc                                      <  2:798572/140‑1 (MQ=255)
                                                                                                       cTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGAc                                      <  2:821493/140‑1 (MQ=255)
                                                                                                       cTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGAc                                      >  2:316515/1‑140 (MQ=255)
                                                                                                       cTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGAc                                      >  2:119527/1‑140 (MQ=255)
                                                                                                       cTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGAc                                      <  2:1624140/140‑1 (MQ=255)
                                                                                                        tAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCCACTTAAGCCCGGCGGTGAAGGGAATGGTGCGTGAGAAAAAATATCCGTCTTCAGCGCACGTCAGCGCGGCGGGCGTCAAGGGAGCTGCCGGGCCgccg                                     >  1:1348080/1‑137 (MQ=255)
                                                                                                        tAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTCAAGGGAGATGCCGGTCCGACt                                     >  1:1348109/1‑140 (MQ=255)
                                                                                                                 aTTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTAc                            >  2:8648/1‑140 (MQ=255)
                                                                                                                 aTTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTAc                            >  2:157026/1‑140 (MQ=255)
                                                                                                                                     ataAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGAt                                                 <  2:1437198/100‑1 (MQ=255)
                                                                                                                                        aaaGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTACGGCGGCGGAAAACGGACAAGTc      <  1:843240/140‑1 (MQ=255)
                                                                                                                                        aaaGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTACGGCGGCGGAAAACGGACAAGTc      <  1:137332/140‑1 (MQ=255)
                                                                                                                                            aCGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTACGGCGGCGGAAAACGGACAAGTCaaaa  >  1:1422249/1‑140 (MQ=255)
                                                                                                                                            aCGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTACGGCGGCGGAAAACGGACAAGTCaaaa  >  1:1462683/1‑140 (MQ=255)
                                                                                                                                            aCGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTACGGCGGCGGAAAACGGACAAGTCaaaa  >  1:1464701/1‑140 (MQ=255)
                                                                                                                                            aCGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTACGGCGGCGGAAAACGGACAAGTCaaaa  >  2:1147174/1‑140 (MQ=255)
                                                                                                                                            aCGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTACGGCGGCGGAAAACGGACAAGTCaaaa  >  1:1750654/1‑140 (MQ=255)
                                                                                                                                            aCGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTACGGCGGCGGAAAACGGACAAGTCaaaa  >  1:414584/1‑140 (MQ=255)
                                                                                                                                            aCGGGCAACTTAAGCCCGGAGGGTACGGGAATGGTGCGCGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGGCTCAGCATTCCGGCGGCGGAAAACGGACAAGTCcaaa  >  1:554089/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                           
ATCCCCCTTGAACATTGAATGAATAGTCATTCATTTTTTGACCAAAAAAAATGAAACTCGTTTCTTCTTTCATCTTAAAAGATTTCCGGTCCGACTTCAATAGCTAATTTGTTATTTTTTTGTATGAAGCTTATAAAAGACGGGCAACTTAAGCCCGGAGGTGAAGGGAATGGTGCGAGAGAAAAAAAATCCGTCTTCAGCGCAAGTCAGCGCGGCGAGCGTAAAGGGAGATGCCGGTCCGACTCAGCATTACGGCGGCGGAAAACGGACAAGTCAAAA  >  NZ_CP034943/3180524‑3180802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: