Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,212,247 C→T 6.0% A129A (GCG→GCA EO946_RS16630 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,212,2470CT 6.0% 479.1 / 13.2 149A129A (GCG→GCAEO946_RS16630ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (82/57);  new base T (5/4);  total (87/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

CTGATCGGCTTTTTTCTCTGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCA  >  NZ_CP034943/3212109‑3212386
                                                                                                                                          |                                                                                                                                           
cTGATCGGCTTTTTTCTCTGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCcgctgacg                                                                                                                                            <  2:453247/140‑1 (MQ=255)
cTGATCGGCTTTTTTCTCTGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCcgctgacg                                                                                                                                            <  2:682679/140‑1 (MQ=255)
cTGATCGGCTTTTTTCTCTGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCcgctgacg                                                                                                                                            <  1:1851234/140‑1 (MQ=255)
        cTTTTTTCTCTGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATg                                                                                                                                    <  2:1768377/140‑1 (MQ=255)
        cTTTTTTCTCTGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATg                                                                                                                                    <  2:1363875/140‑1 (MQ=255)
           ttttCTCTGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGaa                                                                                                                                 >  1:122761/1‑140 (MQ=255)
           ttttCTCTGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCAGCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGaa                                                                                                                                 >  1:124074/1‑140 (MQ=255)
             ttCTCTGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGa                                                                                                                               >  2:572731/1‑140 (MQ=255)
                 ctGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCa                                                                                                                           >  1:2305417/1‑140 (MQ=255)
                  tGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCAt                                                                                                                          >  1:1478898/1‑140 (MQ=255)
                  tGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCAt                                                                                                                          >  1:1682845/1‑140 (MQ=255)
                  tGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGATTGATGGCATACGCTGACGCTGACATGGAAGACTCAt                                                                                                                          >  1:1300826/1‑140 (MQ=255)
                       cGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCa                                                                                                                     <  1:600076/140‑1 (MQ=255)
                                  ccGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATc                                                                                                          <  2:1079377/140‑1 (MQ=255)
                                               ttGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGaa                                                                                             >  1:382689/1‑140 (MQ=255)
                                               ttGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGaa                                                                                             >  1:1979657/1‑140 (MQ=255)
                                                          acCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTtctc                                                                                  <  1:1418592/140‑1 (MQ=255)
                                                          acCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTtctc                                                                                  <  1:2410365/140‑1 (MQ=255)
                                                          acCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTtctc                                                                                  <  1:2329369/140‑1 (MQ=255)
                                                          acCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTtctc                                                                                  <  1:1195875/140‑1 (MQ=255)
                                                          acCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTtctc                                                                                  <  1:166400/140‑1 (MQ=255)
                                                            cTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCaa                                                                                >  1:1593177/1‑140 (MQ=255)
                                                              cGGAGAATGATTGTGCATCATTGACAGTCAGCACTGTAATGCCCGCGTCCTTCAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACg                                                                              >  2:852804/1‑140 (MQ=255)
                                                              cGGAGAATGATTGTGCATCATTGACAGTCAGCACTGTAATGCCCGCGTCCTTCAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACg                                                                              >  2:1156812/1‑140 (MQ=255)
                                                              cGGAGAATGATTGTGCATCATTGACAGTCAGCACTGTAATGCCCGCGTCCTTCAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACg                                                                              >  2:686385/1‑140 (MQ=255)
                                                              cGGAGAATGATTGTGCATCATTGACAGTCAGCACTGTAATGCCCGCGTCCTTCAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACg                                                                              >  2:1963689/1‑140 (MQ=255)
                                                              cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCaatg                                                                              >  1:513485/1‑138 (MQ=255)
                                                              cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACg                                                                              >  2:644948/1‑140 (MQ=255)
                                                              cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACg                                                                              >  2:1293977/1‑140 (MQ=255)
                                                              cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACg                                                                              >  2:1223086/1‑140 (MQ=255)
                                                                gAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGtt                                                                            >  2:2319054/1‑140 (MQ=255)
                                                                gAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGtt                                                                            <  1:1568459/140‑1 (MQ=255)
                                                                    aTGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACa                                                                        <  2:1869127/140‑1 (MQ=255)
                                                                      gATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACAtt                                                                      >  1:2301315/1‑140 (MQ=255)
                                                                        ttGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                    <  1:2256865/140‑1 (MQ=255)
                                                                        ttGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                    <  1:893654/140‑1 (MQ=255)
                                                                        ttGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                    <  2:1797688/140‑1 (MQ=255)
                                                                        ttGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                    <  1:1521002/140‑1 (MQ=255)
                                                                                cGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCg                                                            >  1:811177/1‑140 (MQ=255)
                                                                                cGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCg                                                            >  1:2067349/1‑140 (MQ=255)
                                                                                 gTTGACAGTCAGCACGTTAATGCCCGCGTCTCTCAGCTGTTTGATTGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGTGATCCGGCTTTAGAGAAATGACTTTCTAAACGTTTACATTCATATCTCCGa                                                           <  1:1560102/140‑1 (MQ=255)
                                                                                  ttGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATc                                                                                                          >  2:2311530/1‑92 (MQ=255)
                                                                                         tCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCa                                                   <  2:122720/140‑1 (MQ=255)
                                                                                         tCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCa                                                   <  2:124074/140‑1 (MQ=255)
                                                                                          cAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTGaa                                                  >  1:1899090/1‑140 (MQ=255)
                                                                                            gCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAAcc                                                >  1:699439/1‑140 (MQ=255)
                                                                                                     tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                    >  1:1792595/1‑111 (MQ=255)
                                                                                                     tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                    >  1:1036831/1‑111 (MQ=255)
                                                                                                     tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                    >  1:556876/1‑111 (MQ=255)
                                                                                                     tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                    <  2:556876/111‑1 (MQ=255)
                                                                                                     tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACt                                       <  2:1098572/140‑1 (MQ=255)
                                                                                                       cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCttttt                                           >  2:2377869/1‑134 (MQ=255)
                                                                                                       cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCttttt                                           <  1:2377869/134‑1 (MQ=255)
                                                                                                       cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTc                                     >  1:2143854/1‑140 (MQ=255)
                                                                                                       cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTc                                     >  2:216338/1‑140 (MQ=255)
                                                                                                       cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTc                                     >  1:408880/1‑140 (MQ=255)
                                                                                                       cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATACATAGATCCTACCTGCGAAACCTTTTTGACTTc                                     >  2:775229/1‑140 (MQ=255)
                                                                                                       cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGAATTTCTCAACGTTTACATTCATATCTCGGACCTTCTCAACCTGTTTGAGTTc                                     >  1:378748/1‑140 (MQ=255)
                                                                                                          gcgTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCTACCTTTTTGACTTCCtt                                  >  1:549862/1‑140 (MQ=255)
                                                                                                            gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGtt                                                                            <  2:403850/96‑1 (MQ=255)
                                                                                                            gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGAGTTCCGTTg                                >  1:112711/1‑140 (MQ=255)
                                                                                                            gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTg                                >  1:359704/1‑140 (MQ=255)
                                                                                                            gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTg                                <  1:993604/140‑1 (MQ=255)
                                                                                                            gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTg                                >  1:1745636/1‑140 (MQ=255)
                                                                                                             tctctcAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTgg                               <  2:607737/140‑1 (MQ=255)
                                                                                                              ctctcAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGa                              <  2:408880/140‑1 (MQ=255)
                                                                                                              ccttcAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACGTTTACATTCATATCTCCGACTTTCTCAACCTTTTTGACTTCCTTTGGa                              <  1:852804/137‑1 (MQ=255)
                                                                                                              ccttcAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACGTTTACATTCATATCTCCGACTTTCTCAACCTTTTTGACTTCCTTTGGa                              <  1:686385/137‑1 (MQ=255)
                                                                                                              ccttcAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACGTTTACATTCATATCTCCGACTTTCTCAACCTTTTTGACTTCCTTTGGa                              <  1:1963689/137‑1 (MQ=255)
                                                                                                              ccttcAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACGTTTACATTCATATCTCCGACTTTCTCAACCTTTTTGACTTCCTTTGGa                              <  1:1156812/137‑1 (MQ=255)
                                                                                                               tctcAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGat                             <  2:2187550/140‑1 (MQ=255)
                                                                                                                        tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGAc                                        <  2:620195/120‑1 (MQ=255)
                                                                                                                        tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGAc                                        >  1:620195/1‑120 (MQ=255)
                                                                                                                        tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                    >  1:1037400/1‑140 (MQ=255)
                                                                                                                        tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                    >  1:2175800/1‑140 (MQ=255)
                                                                                                                        tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                    >  1:273641/1‑140 (MQ=255)
                                                                                                                        tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                    >  2:1032153/1‑140 (MQ=255)
                                                                                                                        tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                    >  1:782907/1‑140 (MQ=255)
                                                                                                                        tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCAGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                    >  2:2259217/1‑140 (MQ=255)
                                                                                                                         ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                   <  2:1292466/140‑1 (MQ=255)
                                                                                                                         ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                   <  2:2299821/140‑1 (MQ=255)
                                                                                                                         ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                   <  2:2305417/140‑1 (MQ=255)
                                                                                                                         ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                   <  2:556494/140‑1 (MQ=255)
                                                                                                                         ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                   <  2:1700518/140‑1 (MQ=255)
                                                                                                                         atGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                   <  2:1655853/139‑1 (MQ=255)
                                                                                                                          tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTa                        <  1:2314335/134‑1 (MQ=255)
                                                                                                                          tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGttt                  >  1:753293/1‑140 (MQ=255)
                                                                                                                          tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGttt                  >  1:1104269/1‑140 (MQ=255)
                                                                                                                          tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                   <  1:71945/139‑1 (MQ=255)
                                                                                                                          tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                   >  2:1521909/1‑139 (MQ=255)
                                                                                                                                  tCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGac          >  1:270190/1‑140 (MQ=255)
                                                                                                                                  tCCGCTGACGCTGACATGGAAGACTCATGAGAAAGAACGAGATCCGGCTTTAGAGAAATGACTTTATCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTGGGTGGTGac          >  2:1579465/1‑140 (MQ=255)
                                                                                                                                    cgctgacgctgaCATGGAAGACTCATGAGCAATAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGacac        >  2:550190/1‑140 (MQ=255)
                                                                                                                                    cgctgacgctgaCATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGacac        >  2:277443/1‑140 (MQ=255)
                                                                                                                                    cgctgacgctgaCATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGacac        <  2:273641/140‑1 (MQ=255)
                                                                                                                                    cgctgacgctgaCATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGacac        <  2:2317953/140‑1 (MQ=255)
                                                                                                                                    cgctgacgctgaCATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGacac        >  2:2184254/1‑140 (MQ=255)
                                                                                                                                    cgctgacgctgaCATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGacac        >  2:1519919/1‑140 (MQ=255)
                                                                                                                                    cgctcacgctgaCATGGAAGACTCATGAGCAAGAACGAGATACGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTACCTTTGGATATGTATCGTTTGTGGTGacaa        >  2:1799433/1‑139 (MQ=255)
                                                                                                                                      ctgacgctgacATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTTGTGACACCg      >  2:1997452/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CTGATCGGCTTTTTTCTCTGCCCCGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCA  >  NZ_CP034943/3212109‑3212386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: