Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,212,268 A→G 5.3% A122A (GCT→GCC EO946_RS16630 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,212,2680AG 5.3% 556.8 / 13.7 170A122A (GCT→GCCEO946_RS16630ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (95/66);  new base G (5/4);  total (100/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 170 total aligned reads displayed.

CGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCACCGAGACCG  >  NZ_CP034943/3212132‑3212403
                                                                                                                                        |                                                                                                                                       
cGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCa                                                                                                                                      <  1:600076/140‑1 (MQ=255)
           ccGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGAATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATc                                                                                                                           <  2:141229/140‑1 (MQ=255)
                        ttGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGaa                                                                                                              >  1:1979657/1‑140 (MQ=255)
                        ttGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGaa                                                                                                              >  1:382689/1‑140 (MQ=255)
                        ttGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGaa                                                                                                              >  1:770473/1‑140 (MQ=255)
                                   acCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTtctc                                                                                                   <  1:1195875/140‑1 (MQ=255)
                                   acCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTtctc                                                                                                   <  1:2410365/140‑1 (MQ=255)
                                   acCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTtctc                                                                                                   <  1:166400/140‑1 (MQ=255)
                                   acCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTtctc                                                                                                   <  1:2329369/140‑1 (MQ=255)
                                     cTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCag                                                                                                 >  1:36057/1‑139 (MQ=255)
                                     cTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCaa                                                                                                 >  1:1629780/1‑140 (MQ=255)
                                       cGGAGAATGATTGTGCATCATTGACAGTCAGCACTGTAATGCCCGCGTCCTTCAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACg                                                                                               >  2:1963689/1‑140 (MQ=255)
                                       cGGAGAATGATTGTGCATCATTGACAGTCAGCACTGTAATGCCCGCGTCCTTCAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACg                                                                                               >  2:686385/1‑140 (MQ=255)
                                       cGGAGAATGATTGTGCATCATTGACAGTCAGCACTGTAATGCCCGCGTCCTTCAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACg                                                                                               >  2:852804/1‑140 (MQ=255)
                                       cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCaatg                                                                                               >  1:513485/1‑138 (MQ=255)
                                       cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACg                                                                                               >  1:556494/1‑140 (MQ=255)
                                       cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACg                                                                                               >  1:2299821/1‑140 (MQ=255)
                                       cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACg                                                                                               >  1:2115711/1‑140 (MQ=255)
                                       cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACg                                                                                               >  2:1293977/1‑140 (MQ=255)
                                       cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACg                                                                                               >  2:644948/1‑140 (MQ=255)
                                       cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACg                                                                                               >  1:1572564/1‑140 (MQ=255)
                                         gAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGtt                                                                                             >  2:2319291/1‑140 (MQ=255)
                                             aTGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACa                                                                                         <  2:1869127/140‑1 (MQ=255)
                                             aTGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACa                                                                                         <  2:1206967/140‑1 (MQ=255)
                                             aTGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACa                                                                                         <  2:158853/140‑1 (MQ=255)
                                                 ttGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                                     <  2:1797688/140‑1 (MQ=255)
                                                         cGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCg                                                                             >  1:811177/1‑140 (MQ=255)
                                                          gTTGACAGTCAGCACGTTAATGCCCGCGTCTCTCAGCTGTTTGATTGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGTGATCCGGCTTTAGAGAAATGACTTTCTAAACGTTTACATTCATATCTCCGa                                                                            <  1:1560102/140‑1 (MQ=255)
                                                          gTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGa                                                                            <  1:1942967/140‑1 (MQ=255)
                                                           ttGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATc                                                                                                                           <  1:2311530/92‑1 (MQ=255)
                                                                  tCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCa                                                                    <  2:122720/140‑1 (MQ=255)
                                                                  tCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCa                                                                    <  2:124074/140‑1 (MQ=255)
                                                                     gCAGGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAATACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAAcg                                                                 >  1:430375/1‑139 (MQ=255)
                                                                     gCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAACTGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAAcc                                                                 >  1:1494233/1‑140 (MQ=255)
                                                                     gCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAAcc                                                                 >  1:2237982/1‑140 (MQ=255)
                                                                     gCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAAcc                                                                 >  1:699439/1‑140 (MQ=255)
                                                                     gCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAAcc                                                                 >  2:796173/1‑140 (MQ=255)
                                                                      cACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCt                                                                >  1:2433738/1‑140 (MQ=255)
                                                                              tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                                     <  2:1036831/111‑1 (MQ=255)
                                                                              tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                                     >  1:1036831/1‑111 (MQ=255)
                                                                              tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                                     <  2:556876/111‑1 (MQ=255)
                                                                              tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                                     >  1:1792595/1‑111 (MQ=255)
                                                                              tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACt                                                        <  1:736218/140‑1 (MQ=255)
                                                                              tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACt                                                        <  2:1098572/140‑1 (MQ=255)
                                                                                cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGAt                                                                                                                            <  2:643529/70‑1 (MQ=255)
                                                                                cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTc                                                      >  2:216338/1‑140 (MQ=255)
                                                                                cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTc                                                      >  1:408880/1‑140 (MQ=255)
                                                                                cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTc                                                      >  2:1730036/1‑140 (MQ=255)
                                                                                cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTAATGAGCAAGAACGAGATCCGGCTATAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTc                                                      >  2:2390611/1‑140 (MQ=255)
                                                                                     gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGtt                                                                                             <  2:403850/96‑1 (MQ=255)
                                                                                     gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGAGTTCCGTTg                                                 >  1:112711/1‑140 (MQ=255)
                                                                                     gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTg                                                 <  1:993604/140‑1 (MQ=255)
                                                                                     gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTg                                                 >  1:1745636/1‑140 (MQ=255)
                                                                                     gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTg                                                 >  1:359704/1‑140 (MQ=255)
                                                                                      tctctcAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTgg                                                <  2:607737/140‑1 (MQ=255)
                                                                                       ccttcAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACGTTTACATTCATATCTCCGACTTTCTCAACCTTTTTGACTTCCTTTGGa                                               <  1:852804/137‑1 (MQ=255)
                                                                                       ccttcAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACGTTTACATTCATATCTCCGACTTTCTCAACCTTTTTGACTTCCTTTGGa                                               <  1:1156812/137‑1 (MQ=255)
                                                                                        tctcAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGat                                              <  2:2187728/140‑1 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGAc                                                         >  1:620195/1‑120 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                                     >  2:2305592/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                                     >  1:1037400/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                                     >  1:782907/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                                     >  1:2175800/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                                     >  1:273641/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCAGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                                     >  2:2259217/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGATTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCATTTGGATATGTATCGt                                     >  2:404740/1‑140 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAATGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                    <  2:513485/140‑1 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                    <  2:1292466/140‑1 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                    <  2:563487/140‑1 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                    <  2:1572564/140‑1 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                    <  2:1700518/140‑1 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                    <  2:11096/140‑1 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                    <  2:2299821/140‑1 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGa                                                                                                          >  2:1856479/1‑69 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTa                                         <  1:2314335/134‑1 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTa                                         >  2:2314335/1‑134 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGttt                                   >  1:1104269/1‑140 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                    >  2:71945/1‑139 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                    <  1:1266034/139‑1 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                    <  1:1521909/139‑1 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                    >  2:1521909/1‑139 (MQ=255)
                                                                                                           tCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGac                           >  1:694640/1‑140 (MQ=255)
                                                                                                             cgctgacgctgaCATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGacac                         <  2:2317953/140‑1 (MQ=255)
                                                                                                             cgctgacgctgaCATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGacac                         <  2:273641/140‑1 (MQ=255)
                                                                                                             cgctgacgctgaCATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGacac                         >  2:2184254/1‑140 (MQ=255)
                                                                                                             cgctgacgctgaCATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGacac                         >  2:550752/1‑140 (MQ=255)
                                                                                                               ctgacgctgacATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTTGTGACACCg                       >  2:1997452/1‑140 (MQ=255)
                                                                                                                 gacgctgacATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGAc                     <  1:2024742/140‑1 (MQ=255)
                                                                                                                   cgctgacATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCa                   <  2:2115711/140‑1 (MQ=255)
                                                                                                                       gacATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACttt               <  1:644948/140‑1 (MQ=255)
                                                                                                                       gacATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACttt               <  1:1293977/140‑1 (MQ=255)
                                                                                                                              aaGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccg        >  1:909605/1‑140 (MQ=255)
                                                                                                                              aaGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccg        >  1:1792712/1‑140 (MQ=255)
                                                                                                                              aaGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccg        >  1:1859600/1‑140 (MQ=255)
                                                                                                                               aGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccga       <  1:1366491/140‑1 (MQ=255)
                                                                                                                               aGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccga       <  1:2390705/140‑1 (MQ=255)
                                                                                                                               aGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccga       <  2:770473/140‑1 (MQ=255)
                                                                                                                                  cTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccgatac    >  2:1133711/1‑140 (MQ=255)
                                                                                                                                  cTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTAATATCTCCGACCTTCTCAACCTTATTGACTTCCTGTGGATATGTATCGTTTGTGGTGAGACCGACCACTTTGTCaccgagac    >  2:1209737/1‑140 (MQ=255)
                                                                                                                                    cATGAGCAAGAACGAGTTCCGGCTTTAGAGAAATGACTTTCTCAACGTTTAAATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGAAACAGACCACTTTGTCaccgagaacg  >  2:330562/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCACCGAGACCG  >  NZ_CP034943/3212132‑3212403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: