Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,212,271 A→C 5.3% L121L (CTT→CTG EO946_RS16630 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,212,2710AC 5.3% 553.5 / 12.7 170L121L (CTT→CTGEO946_RS16630ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (95/66);  new base C (5/4);  total (100/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 172 total aligned reads displayed.

CGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCACCGAGACCGAGAGCG  >  NZ_CP034943/3212132‑3212409
                                                                                                                                           |                                                                                                                                          
cGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCa                                                                                                                                            <  1:600076/140‑1 (MQ=255)
           ccGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATc                                                                                                                                 <  2:1079377/140‑1 (MQ=255)
                        ttGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGaa                                                                                                                    >  1:1979657/1‑140 (MQ=255)
                                   acCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTtctc                                                                                                         <  1:1195875/140‑1 (MQ=255)
                                   acCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTtctc                                                                                                         <  1:1342732/140‑1 (MQ=255)
                                   acCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTtctc                                                                                                         <  1:2410365/140‑1 (MQ=255)
                                     cTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCaa                                                                                                       >  1:1629780/1‑140 (MQ=255)
                                       cGGAGAATGATTGTGCATCATTGACAGTCAGCACTGTAATGCCCGCGTCCTTCAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACg                                                                                                     >  2:852804/1‑140 (MQ=255)
                                       cGGAGAATGATTGTGCATCATTGACAGTCAGCACTGTAATGCCCGCGTCCTTCAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACg                                                                                                     >  2:686385/1‑140 (MQ=255)
                                       cGGAGAATGATTGTGCATCATTGACAGTCAGCACTGTAATGCCCGCGTCCTTCAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACg                                                                                                     >  2:1156812/1‑140 (MQ=255)
                                       cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACg                                                                                                     >  1:2115711/1‑140 (MQ=255)
                                       cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACg                                                                                                     >  2:1293977/1‑140 (MQ=255)
                                       cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACg                                                                                                     >  1:1572564/1‑140 (MQ=255)
                                       cGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACg                                                                                                     >  2:644948/1‑140 (MQ=255)
                                         gAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGtt                                                                                                   >  2:2319291/1‑140 (MQ=255)
                                             aTGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACa                                                                                               <  2:158853/140‑1 (MQ=255)
                                               gATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACAtt                                                                                             >  1:2301315/1‑140 (MQ=255)
                                                 ttGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                                           <  1:893654/140‑1 (MQ=255)
                                                 ttGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                                           <  2:1797688/140‑1 (MQ=255)
                                                 ttGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                                           <  1:2256865/140‑1 (MQ=255)
                                                         cGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCg                                                                                   >  1:2067349/1‑140 (MQ=255)
                                                          gTTGACAGTCAGCACGTTAATGCCCGCGTCTCTCAGCTGTTTGATTGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGTGATCCGGCTTTAGAGAAATGACTTTCTAAACGTTTACATTCATATCTCCGa                                                                                  <  1:1560102/140‑1 (MQ=255)
                                                          gTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGa                                                                                  <  1:1942967/140‑1 (MQ=255)
                                                           ttGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATc                                                                                                                                 <  1:2311530/92‑1 (MQ=255)
                                                           ttGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATc                                                                                                                                 >  2:2311530/1‑92 (MQ=255)
                                                                aGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTtct                                                                            >  1:1733669/1‑140 (MQ=255)
                                                                  tCAGCTCGGTAATGCCAGCGTCTCTCAGCTGTTTGATGGCATACGCTGAAGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCa                                                                          <  2:122761/140‑1 (MQ=255)
                                                                  tCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCa                                                                          <  2:122720/140‑1 (MQ=255)
                                                                     gCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAACTGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAAcc                                                                       >  1:1494233/1‑140 (MQ=255)
                                                                     gCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAAcc                                                                       >  2:796173/1‑140 (MQ=255)
                                                                              tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                                           >  1:1792595/1‑111 (MQ=255)
                                                                              tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                                           >  1:1036831/1‑111 (MQ=255)
                                                                              tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                                           >  1:556876/1‑111 (MQ=255)
                                                                              tGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCa                                                                                           <  2:1792595/111‑1 (MQ=255)
                                                                                cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGAt                                                                                                                                  >  1:643529/1‑70 (MQ=255)
                                                                                cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCttttt                                                                  <  1:2377869/134‑1 (MQ=255)
                                                                                cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCttttt                                                                  >  2:2377869/1‑134 (MQ=255)
                                                                                cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTc                                                            >  1:408880/1‑140 (MQ=255)
                                                                                cccGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGAATTTCTCAACGTTTACATTCATATCTCGGACCTTCTCAACCTGTTTGAGTTc                                                            >  1:378748/1‑140 (MQ=255)
                                                                                   gcgTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCTACCTTTTTGACTTCCtt                                                         >  1:549862/1‑140 (MQ=255)
                                                                                     gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGATATCCGGCTTTAGAGAAAAGACTTTCTCAACGATTACATTCATATCTCAGACCTTCTCAACATTTTTGACTTCATTTg                                                       >  2:373479/1‑140 (MQ=255)
                                                                                     gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGtt                                                                                                   >  1:403850/1‑96 (MQ=255)
                                                                                     gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGAGTTCCGTTg                                                       >  1:112711/1‑140 (MQ=255)
                                                                                     gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTg                                                       <  1:993604/140‑1 (MQ=255)
                                                                                     gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTg                                                       >  1:1745636/1‑140 (MQ=255)
                                                                                     gTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTg                                                       >  1:359704/1‑140 (MQ=255)
                                                                                      tctctcAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTgg                                                      <  2:2180460/140‑1 (MQ=255)
                                                                                      tctctcAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTgg                                                      <  2:607737/140‑1 (MQ=255)
                                                                                       ccttcAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACGTTTACATTCATATCTCCGACTTTCTCAACCTTTTTGACTTCCTTTGGa                                                     <  1:1156812/137‑1 (MQ=255)
                                                                                       ccttcAGCTGTTTGATGGCATCCGCTGATGCTGACATGGAAGACTCATGGGCCAGAACGAGATCTGGTTTTAGAGAAATGACTTTTTCAACGTTTACATTCATATCTCCGACTTTCTCAACCTTTTTGACTTCCTTTGGa                                                     <  1:686385/137‑1 (MQ=255)
                                                                                        tctcAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGat                                                    <  2:2187550/140‑1 (MQ=255)
                                                                                        tctcAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGat                                                    <  2:2187728/140‑1 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGAc                                                               <  2:620195/120‑1 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                                           >  1:1037400/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                                           >  2:1913970/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                                           >  1:2175800/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                                           >  1:782907/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                                           >  2:1032153/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACATTCTCAACCTTTTTGACTTAATTTGGATATGTATCGt                                           >  1:294922/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCAGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                                           >  2:2259217/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCGCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGt                                           >  1:2317953/1‑140 (MQ=255)
                                                                                                 tttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGATTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCATTTGGATATGTATCGt                                           >  2:404740/1‑140 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAATGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                          <  2:513485/140‑1 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                          <  2:563487/140‑1 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                          <  2:2305417/140‑1 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                          <  2:2299821/140‑1 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                          <  2:556494/140‑1 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                          <  2:1700518/140‑1 (MQ=255)
                                                                                                  ttGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                          <  2:11096/140‑1 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGa                                                                                                                <  1:1856479/69‑1 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGa                                                                                                                >  2:1856479/1‑69 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGttt                                         >  1:1104269/1‑140 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGttt                                         >  1:753293/1‑140 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                          <  1:71945/139‑1 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                          >  2:71945/1‑139 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                          <  1:1266034/139‑1 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                          >  2:1521909/1‑139 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                          <  1:1521909/139‑1 (MQ=255)
                                                                                                   tGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGtt                                          >  2:1266034/1‑139 (MQ=255)
                                                                                                           tCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGac                                 >  1:694640/1‑140 (MQ=255)
                                                                                                           tCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGac                                 >  1:270190/1‑140 (MQ=255)
                                                                                                           tCCGCTGACGCTGACATGGAAGACTCATGAGAAAGAACGAGATCCGGCTTTAGAGAAATGACTTTATCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTGGGTGGTGac                                 >  2:1579465/1‑140 (MQ=255)
                                                                                                             cgctgacgctgaCATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGacac                               >  2:550752/1‑140 (MQ=255)
                                                                                                             cgctgacgctgaCATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGacac                               <  2:2317953/140‑1 (MQ=255)
                                                                                                             cgctgacgctgaCATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGacac                               <  2:273641/140‑1 (MQ=255)
                                                                                                               ctgacgctgacATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTTGTGACACCg                             >  2:1997452/1‑140 (MQ=255)
                                                                                                                  acgctgacATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAACTGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGAcc                          >  1:813820/1‑140 (MQ=255)
                                                                                                                   cgctgacATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCa                         <  2:2115711/140‑1 (MQ=255)
                                                                                                                              aaGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccg              >  1:1859600/1‑140 (MQ=255)
                                                                                                                              aaGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccg              >  1:798370/1‑140 (MQ=255)
                                                                                                                              aaGACTCATGAGAAAGAACGAGATCCGGCTTTAGATAAATGACTTTATCAACGTTTACAATCATATCTCCGACCTTCTCAACCTTGTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccg              >  1:471779/1‑140 (MQ=255)
                                                                                                                               aGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccga             <  1:1366491/140‑1 (MQ=255)
                                                                                                                               aGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccga             <  1:2390705/140‑1 (MQ=255)
                                                                                                                               aGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccga             <  2:1979657/140‑1 (MQ=255)
                                                                                                                               aGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccga             <  2:770473/140‑1 (MQ=255)
                                                                                                                                  cTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGAACACTTTGTCaccgagac          >  2:1127202/1‑140 (MQ=255)
                                                                                                                                  cTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAAACTTTTTGACTTCGTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCaccgagac          >  2:1294074/1‑140 (MQ=255)
                                                                                                                                  cTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTAATATCTCCGACCTTCTCAACCTTATTGACTTCCTGTGGATATGTATCGTTTGTGGTGAGACCGACCACTTTGTCaccgagac          >  2:1209737/1‑140 (MQ=255)
                                                                                                                                    cATGAGCAAGAACGAGTTCCGGCTTTAGAGAAATGACTTTCTCAACGTTTAAATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGAAACAGACCACTTTGTCaccgagaacg        >  2:330562/1‑140 (MQ=255)
                                                                                                                                          cAAGAACGAGATCCGGCTTTAGAGAAATGACTGTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCACCGAGACCGAGAGCg  <  2:1417133/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CGGCAGCTTCTCCGATCATTTCAATTGATTTGTACACCTCGGAAAATGATTGTGCATCGTTGACAGTCAGCACGGTAATGCCCGCGTCTCTCAGCTGTTTGATGGCATCCGCTGACGCTGACATGGAAGACTCATGAGCAAGAACGAGATCCGGCTTTAGAGAAATGACTTTCTCAACGTTTACATTCATATCTCCGACCTTCTCAACCTTTTTGACTTCCTTTGGATATGTATCGTTTGTGGTGACACCGACCACTTTGTCACCGAGACCGAGAGCG  >  NZ_CP034943/3212132‑3212409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: