BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,285,491	T→C	5.4%	I304V (ATT→GTT)	EO946_RS16995 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,285,491	0	T	C	5.4%	875.4 / 25.2	291	I304V (ATT→GTT)	EO946_RS16995	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base T (157/118); new base C (10/6); total (167/124)
Fisher's exact test for biased strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

TGGCGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAT  >  NZ_CP034943/3285356‑3285629

   cGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGAGTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCGTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTc                                                                                                                                     >  2:326426/1‑140 (MQ=21)

   cGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTc                                                                                                                                     >  2:133414/1‑140 (MQ=34)

   cGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTc                                                                                                                                     >  2:1028739/1‑140 (MQ=34)

     gTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTg                                                                                                                                   >  1:744922/1‑140 (MQ=34)

      tCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGa                                                                                                                                  <  2:578217/140‑1 (MQ=34)

      tCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGa                                                                                                                                  <  1:2062119/140‑1 (MQ=34)

       cGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGAt                                                                                                                                 >  1:1937474/1‑140 (MQ=34)

        gTGGTTCGATCAATGCTGTTAAGATAGCACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATa                                                                                                                                <  2:156293/140‑1 (MQ=21)

             tCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGt                                                                                                                           <  2:514910/140‑1 (MQ=34)

             tCGATCAATGCTGTTAAGAGAGAACGTTTCAGACTTTAGTGAGGACTGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGt                                                                                                                           <  2:2428264/140‑1 (MQ=21)

                aTCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGAt                                                                                                                                 >  2:1824593/1‑131 (MQ=34)

                aTCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGAt                                                                                                                                 <  1:1824593/131‑1 (MQ=34)

                aTCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAg                                                                                                                        >  2:2373802/1‑140 (MQ=34)

                aTCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAg                                                                                                                        >  2:2165144/1‑140 (MQ=34)

                aTCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCGTTTGTGTTAATAGCTTTCTGATACTCGTAAg                                                                                                                        >  1:880285/1‑140 (MQ=21)

                     tGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGAGTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGta                                                                                                                   <  2:47771/140‑1 (MQ=21)

                          ttAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATacac                                                                                                              >  2:1077028/1‑140 (MQ=34)

                          ttAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATacac                                                                                                              >  2:2223404/1‑140 (MQ=34)

                          ttAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATacac                                                                                                              >  2:2032904/1‑140 (MQ=34)

                           tAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATAcaca                                                                                                             <  1:984072/140‑1 (MQ=34)

                               aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAg                                                                                                            <  1:88878/137‑1 (MQ=34)

                               aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAc                                                                                                         >  1:336498/1‑140 (MQ=34)

                               aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAc                                                                                                         >  1:2092999/1‑140 (MQ=34)

                               aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAc                                                                                                         >  1:1955099/1‑140 (MQ=34)

                               aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAc                                                                                                         >  1:1582278/1‑140 (MQ=34)

                               aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAc                                                                                                         >  2:2386998/1‑140 (MQ=34)

                               aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAc                                                                                                         >  1:1196553/1‑140 (MQ=34)

                                tAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAcc                                                                                                        <  2:70934/140‑1 (MQ=34)

                                tAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAcc                                                                                                        <  2:974641/140‑1 (MQ=34)

                                  gAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCtt                                                                                                      >  2:908777/1‑140 (MQ=34)

                                  gAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCtt                                                                                                      >  1:1625641/1‑140 (MQ=34)

                                  gAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCtt                                                                                                      >  2:397682/1‑140 (MQ=34)

                                   aaCGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTg                                                                                                     >  1:94332/1‑140 (MQ=34)

                                    aCGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTgg                                                                                                    >  1:1615129/1‑140 (MQ=34)

                                    aCGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGACTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTgg                                                                                                    >  1:525119/1‑140 (MQ=21)

                                    aCGGTTCAGAATTTAGTGAGGAATGAAACGATTCAATGGAGGCATTATCAGCGGGCCTCCCTTTGCGGGACATGATCATGTTAATGCCTTATTTGTTAATAGCTTTCTGATACTCGTAAGAAGTATACACAGAACCTTgg                                                                                                    >  2:1706469/1‑140 (MQ=11)

                                     cGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGt                                                                                                   <  2:263951/140‑1 (MQ=34)

                                     cGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGt                                                                                                   <  2:12828/140‑1 (MQ=34)

                                     cGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGt                                                                                                   <  1:1982581/140‑1 (MQ=34)

                                     cGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGt                                                                                                   <  2:879175/140‑1 (MQ=34)

                                     cGTTTCAGACTTTAGGGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGt                                                                                                   <  1:213675/140‑1 (MQ=21)

                                       tttCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCa                                                                                                 <  2:380116/140‑1 (MQ=34)

                                       tttCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCa                                                                                                 <  2:808964/140‑1 (MQ=34)

                                        ttCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGta                                                                                                                   >  2:438292/1‑121 (MQ=35)

                                        ttCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCAc                                                                                                >  2:2355111/1‑140 (MQ=34)

                                         tCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACt                                                                                               <  1:2316244/140‑1 (MQ=34)

                                              cTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTa                                                                                          <  2:1625641/140‑1 (MQ=34)

                                                   gTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACg                                                                                     <  1:132398/140‑1 (MQ=34)

                                                   gTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACg                                                                                     <  1:348608/140‑1 (MQ=34)

                                                    tGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACt                                                                                               <  1:558725/129‑1 (MQ=35)

                                                    tGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGc                                                                                    <  1:1499119/140‑1 (MQ=34)

                                                    tGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGc                                                                                    <  1:1756435/140‑1 (MQ=34)

                                                    tGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGc                                                                                    <  2:843573/140‑1 (MQ=34)

                                                    tGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGc                                                                                    <  2:1397524/140‑1 (MQ=34)

                                                         aaTGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTtc                                                                               >  1:1005019/1‑140 (MQ=34)

                                                                               aTTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTaa                                                                                                                         >  1:1694131/1‑76 (MQ=34)

                                                                                ttATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGc                                                                                    <  2:499364/112‑1 (MQ=32)

                                                                                    cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTTATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  2:2304528/140‑1 (MQ=17)

                                                                                    cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  1:217101/140‑1 (MQ=32)

                                                                                    cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  2:1937474/140‑1 (MQ=32)

                                                                                    cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  1:2024927/140‑1 (MQ=32)

                                                                                    cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  1:1892408/140‑1 (MQ=32)

                                                                                       cGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATg                                                 >  2:1301291/1‑140 (MQ=34)

                                                                                         ggCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTc                                               >  1:140869/1‑140 (MQ=34)

                                                                                         ggCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTc                                               >  1:420683/1‑140 (MQ=34)

                                                                                         ggCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTc                                               >  2:167302/1‑140 (MQ=34)

                                                                                         ggCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTGTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGAAGTTATCAGGCAGTGTTGGCAGCTGATTAAGTATGTc                                               >  1:1348951/1‑140 (MQ=255)

                                                                                                 tttGCGGGACATTCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTAGACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACaa                                       <  1:186701/140‑1 (MQ=21)

                                                                                                 tttGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACaa                                       <  1:440288/140‑1 (MQ=34)

                                                                                                 tttGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACaa                                       <  1:778630/140‑1 (MQ=34)

                                                                                                 tttGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACaa                                       <  2:1207323/140‑1 (MQ=34)

                                                                                                          cATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACaa                                       <  1:2404485/131‑1 (MQ=34)

                                                                                                           aTGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTc                             <  2:2063048/140‑1 (MQ=34)

                                                                                                             gCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCt                           >  1:668361/1‑140 (MQ=34)

                                                                                                             gCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCt                           >  1:684706/1‑140 (MQ=34)

                                                                                                             gCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCt                           >  2:1581283/1‑140 (MQ=34)

                                                                                                                  tGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTa                      >  1:38581/1‑140 (MQ=34)

                                                                                                                  tGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTa                      >  2:1180131/1‑140 (MQ=34)

                                                                                                                  tGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTa                      >  1:464292/1‑140 (MQ=34)

                                                                                                                       aTGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATcccc                 <  1:1776689/140‑1 (MQ=39)

                                                                                                                        tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCtg                                 <  1:83247/123‑1 (MQ=35)

                                                                                                                        tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCtg                                 >  2:1492166/1‑123 (MQ=35)

                                                                                                                        tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCa                >  1:1086277/1‑140 (MQ=40)

                                                                                                                        tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCa                >  2:1762983/1‑140 (MQ=40)

                                                                                                                        tGCCTTTTGTGTTAATAGCTTTCTGATACTAGTAAGATGTATACACAAAACCTTGGTCACTATGTAACAAGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAACTATTTCTAACACAAAGTCTGTGTCCTGCTTATACCCa                >  2:1114620/1‑140 (MQ=11)

                                                                                                                            ttttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCg            <  2:1982914/140‑1 (MQ=255)

                                                                                                                             tttGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt           >  1:2014614/1‑140 (MQ=37)

                                                                                                                             tttGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt           >  1:1287141/1‑140 (MQ=255)

                                                                                                                             tttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt           >  2:1623933/1‑140 (MQ=255)

                                                                                                                             tttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt           >  2:1453158/1‑140 (MQ=255)

                                                                                                                              ttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTa          <  1:299809/140‑1 (MQ=255)

                                                                                                                              ttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTa          <  1:1115097/140‑1 (MQ=255)

                                                                                                                               tgtgTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  1:501103/97‑1 (MQ=33)

                                                                                                                               tgtgTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTaa         >  2:2114502/1‑140 (MQ=255)

                                                                                                                                   ttAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGc     >  1:2276960/1‑140 (MQ=255)

                                                                                                                                   ttAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACTCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGc     >  2:1211386/1‑140 (MQ=11)

                                                                                                                                   ttAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGc     >  2:2162427/1‑140 (MQ=255)

                                                                                                                                   ttAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGc     >  2:1615624/1‑140 (MQ=255)

                                                                                                                                    tAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAATCa    >  1:1459329/1‑140 (MQ=255)

                                                                                                                                    tAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCa    >  1:2189306/1‑140 (MQ=255)

TGGCGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAT  >  NZ_CP034943/3285356‑3285629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`