Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,308,421 A→G 8.8% G194G (GGT→GGC EO946_RS17110 ← glycosyl hydrolase family 18 protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,308,4210AG 8.8% 270.0 / 13.0 91G194G (GGT→GGCEO946_RS17110glycosyl hydrolase family 18 protein
Reads supporting (aligned to +/- strand):  ref base A (47/36);  new base G (4/4);  total (51/40)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

ACTTTATTGGCTTTGATTTTTTTGACTGAAAACTGCAGAGAGCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAG  >  NZ_CP034943/3308287‑3308553
                                                                                                                                      |                                                                                                                                    
aCTTTATTGGGTTTGATTTTTTTGACTGAAAACTGCAGAGAGCTGTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCGCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGAt                                                                                                                                 <  2:745086/140‑1 (MQ=255)
   ttATTGGCTTTGATTTTTTTGACTGAAAACTGCAGAGAGCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGAtttt                                                                                                                              <  1:1205710/140‑1 (MQ=255)
   ttATTGGCTTTGATTTTTTTGACTGAAAACTGCAGAGAGCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGAtttt                                                                                                                              <  1:2157589/140‑1 (MQ=255)
   ttATTGGCTTTGATTTTTTTGACTGAAAACTGCAGAGAGCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGAtttt                                                                                                                              <  1:2411685/140‑1 (MQ=255)
    tATTGGCTTTGATTTTTTTGACTGAAAACTGCAGAGAGCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTc                                                                                                                             >  1:19574/1‑140 (MQ=255)
    tATTGGCTTTGATTTTTTTGACTGAAAACTGCAGAGAGCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTc                                                                                                                             >  1:862829/1‑140 (MQ=255)
    tATTGGCTTTGATTTTTTTGACTGAAAACTGCAGAGAGCTTTTGATCCAGCTTGTGATCGCCACTGATCCCGGATCGCCCCAAATGCCGTCTTCATCGTAAGACATCACCTGCACGTAATAAGCATATTTACCGATTTTc                                                                                                                             >  1:19701/1‑140 (MQ=255)
       tGGCTTTGATTTTTTTGACTGAAAACTGCAGAGAGCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCa                                                                                                                          <  1:2002500/140‑1 (MQ=255)
                   ttttGACTGAAAACTGCAGAGAGCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGc                                                                                                              <  1:457257/140‑1 (MQ=255)
                    tttGACTGAAAACTGCAGAGAGCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGcc                                                                                                             <  1:1328623/140‑1 (MQ=255)
                               aCTGCAGAGAGCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGtca                                                                                                  <  2:787696/140‑1 (MQ=255)
                                        agCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGa                                                                                         >  2:1260386/1‑140 (MQ=255)
                                          cTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTAcc                                                                                                                                    >  1:1464814/1‑95 (MQ=255)
                                          cTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTAcc                                                                                                                                    <  2:1464814/95‑1 (MQ=255)
                                           ttttGATCCAGTTTGTGCTTGCCACTGACCCCGGCTCACCCCAAATGCCGTGTTCATCGTAGGTCATGACTTGTACGAAGTCGGCATATTTGCCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTTatca                                                                                      >  1:595367/1‑140 (MQ=255)
                                           ttttGATCCAGTTTGTGCTTGCCACTGACCCCGGCTCACCCCAAATGCCGTGTTCATCGTAGGTCATGACTTGTACGAAGTCGGCATATTTGCCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTTatca                                                                                      >  1:2172943/1‑140 (MQ=255)
                                           ttttGATCCAGTTTGTGCTTGCCACTGACCCCGGCTCACCCCAAATGCCGTGTTCATCGTAGGTCATGACTTGTACGAAGTCGGCATATTTGCCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTTatca                                                                                      >  1:1231461/1‑140 (MQ=255)
                                           ttttGATCCAGTTTGTGCTTGCCACTGACCCCGGCTCACCCCAAATGCCGTGTTCATCGTAGGTCATGACTTGTACGAAGTCGGCATATTTGCCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTTatca                                                                                      >  1:1231434/1‑140 (MQ=255)
                                             ttGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGatcatc                                                                                    >  2:1190624/1‑140 (MQ=255)
                                             ttGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCAGCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGatcatc                                                                                    >  2:2029964/1‑140 (MQ=255)
                                                    aGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCtt                                                                             >  2:1511284/1‑140 (MQ=255)
                                                    aGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCtt                                                                             >  2:1353881/1‑140 (MQ=255)
                                                    aGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCtt                                                                             >  2:1913929/1‑140 (MQ=255)
                                                               gCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCCGCTCCCGTCATCATTTTGATCATCGGCGCTTTTGGCAGGTAc                                                                  >  1:122269/1‑140 (MQ=255)
                                                               gCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAAc                                                                  <  1:2250483/140‑1 (MQ=255)
                                                               gCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAAc                                                                  <  1:464008/140‑1 (MQ=255)
                                                                  aCTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGa                                                               <  2:1816240/140‑1 (MQ=255)
                                                                       tCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCa                                                          <  1:757665/140‑1 (MQ=255)
                                                                       tCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCa                                                          <  1:934350/140‑1 (MQ=255)
                                                                       tCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCa                                                          <  1:1604087/140‑1 (MQ=255)
                                                                       tCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCa                                                          <  2:347236/140‑1 (MQ=255)
                                                                       tCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCa                                                          <  2:566421/140‑1 (MQ=255)
                                                                       tCCCGGATCGCCCCAAATGCCGTGTTCATCGAAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCa                                                          <  2:609147/140‑1 (MQ=255)
                                                                         ccGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCAtt                                                        >  1:546541/1‑140 (MQ=255)
                                                                                         gCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCt                                        <  1:2097847/140‑1 (MQ=255)
                                                                                         gCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCt                                        <  1:1406341/140‑1 (MQ=255)
                                                                                            ttgtTCATCGTAAGTAATCACCAGCACATAATCCGCAAATTTAACGATTTAAGCATAATCATACCGCCAGCTCCAGTCATCATATTGATCATCGGCGATTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCtttt                                     >  1:440551/2‑140 (MQ=255)
                                                                                            gtgtTCATCTTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCtttt                                     >  2:1223968/1‑140 (MQ=255)
                                                                                            gtgtTCATCGTAAGTCATCACCTGCACGTCATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCCTCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCtttt                                     >  2:795675/1‑140 (MQ=255)
                                                                                            gtgtTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCCTTGTTTTAATATGTTTCtttt                                     >  1:2376764/1‑140 (MQ=255)
                                                                                            gtgtTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCtttt                                     >  1:719249/1‑140 (MQ=255)
                                                                                            gtgtTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCtttt                                     >  1:1078750/1‑140 (MQ=255)
                                                                                            gtgtTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCtttt                                     >  2:2288749/1‑140 (MQ=255)
                                                                                            gtgtTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCtttt                                     >  1:1017286/1‑140 (MQ=255)
                                                                                            gtgtTCATCGTAAGTCATCACCTGCACGTAAGCCGCATATTTACCGATTTTCGCAGAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAAGCAGTGTGTTAATATGTTTGtttt                                     >  1:1267186/1‑140 (MQ=255)
                                                                                               ttCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCa                                  <  2:19701/140‑1 (MQ=255)
                                                                                               ttCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCa                                  <  2:19574/140‑1 (MQ=255)
                                                                                               ttCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCa                                  <  1:1534451/140‑1 (MQ=255)
                                                                                               ttCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCa                                  <  2:128684/140‑1 (MQ=255)
                                                                                                  aTCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCaga                                <  1:1510997/139‑1 (MQ=255)
                                                                                                  aTCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCaga                                >  2:1510997/1‑139 (MQ=255)
                                                                                                    cGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGcc                             >  2:1204194/1‑140 (MQ=255)
                                                                                                    cGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGcc                             >  2:1392304/1‑140 (MQ=255)
                                                                                                    cGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGcc                             >  2:845081/1‑140 (MQ=255)
                                                                                                    cGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCAGTGTTTTAATATGTTTCTTTTTCAGAGcc                             >  1:1869057/1‑140 (MQ=255)
                                                                                                         gTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTga                        >  2:764896/1‑140 (MQ=255)
                                                                                                         gTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTga                        >  2:1072349/1‑140 (MQ=255)
                                                                                                            atcaCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGAc                     >  2:289955/1‑140 (MQ=255)
                                                                                                            atcaCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGAc                     >  2:962546/1‑140 (MQ=255)
                                                                                                            atcaCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGAc                     >  2:1386243/1‑140 (MQ=255)
                                                                                                            atcaCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGAc                     >  2:962626/1‑140 (MQ=255)
                                                                                                            atcaCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGAc                     >  2:1016897/1‑140 (MQ=255)
                                                                                                            atcaCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTAATAGCCTGTGAGAc                     >  2:1523583/1‑140 (MQ=255)
                                                                                                            atcaCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATAAGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGACTGTGAGAc                     >  2:2329472/1‑140 (MQ=255)
                                                                                                              caCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACAt                   >  2:54011/1‑140 (MQ=255)
                                                                                                              caCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACAt                   >  2:2362854/1‑140 (MQ=255)
                                                                                                                    cACGTAATCCGCATATTTACCGATTTTCGCCTAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTtgaa             >  1:2059866/1‑140 (MQ=255)
                                                                                                                    cACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGTACCCATTGTTTTAATATGTTTCTTTTTCAGGGCCTGTGAGACATCTtgaa             >  1:779278/1‑140 (MQ=255)
                                                                                                                    cACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGATCCTGTGAGACATCtttat             >  1:2255066/1‑137 (MQ=255)
                                                                                                                    cACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTtgaa             >  2:1137497/1‑140 (MQ=255)
                                                                                                                    cACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGGGTGAATAGGTGTCTTTTTCAGAGCCTGTGAGACATCTtgaa             >  1:2255156/1‑140 (MQ=255)
                                                                                                                         agtcggCATATTTGCCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTTATCATCGGCGCTTTTGGCCGTAACGGATACCATTGTTTTAATATGTTTCTTATTCAAAGCCTGTGAGACATATTGAATGAAg        <  2:595367/135‑1 (MQ=255)
                                                                                                                         agtcggCATATTTGCCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTTATCATCGGCGCTTTTGGCCGGAACGGATACCATTGTTTTAATATGTTTCTTATTCAAAGCCTGTGAGACATATTGAATGAAg        <  2:2172943/135‑1 (MQ=255)
                                                                                                                         agtcggCATATTTGCCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTTATCATCGGCGCTTTTGGCCGGAACGGATACCATTGTTTTAATATGTTTCTTATTCAAAGCCTGTGAGACATATTGAATGAAg        <  2:1231461/135‑1 (MQ=255)
                                                                                                                         agtcggCATATTTGCCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTTATCATCGGCGCTTTTGGCCGGAACGGATACCATTGTTTTAATATGTTTCTTATTCAAAGCCTGTGAGACATATTGAATGAAg        <  2:1231434/135‑1 (MQ=255)
                                                                                                                         aaTCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAg        <  2:2393227/140‑1 (MQ=255)
                                                                                                                             cGCCTATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg    <  1:1087823/140‑1 (MQ=255)
                                                                                                                             cGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg    <  1:1403754/140‑1 (MQ=255)
                                                                                                                             cGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg    <  1:1353881/140‑1 (MQ=255)
                                                                                                                             cGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg    <  1:1913929/140‑1 (MQ=255)
                                                                                                                             cGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg    <  1:1497919/140‑1 (MQ=255)
                                                                                                                             cGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg    <  1:275271/140‑1 (MQ=255)
                                                                                                                             cGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg    <  1:1511284/140‑1 (MQ=255)
                                                                                                                             cGCATATTTACCGATGTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg    <  1:1352489/140‑1 (MQ=255)
                                                                                                                             cGCATATTTACCGAGTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg    <  2:300278/140‑1 (MQ=255)
                                                                                                                              gCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCAt                                                         <  2:982328/86‑1 (MQ=255)
                                                                                                                              gCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCAt                                                         >  1:982328/1‑86 (MQ=255)
                                                                                                                               cATATTTACCGATTTTCGCCTAATCATACGGCCAGCTCCCGTCATCATGTTGATCATCGGCGCTTTTGGCAGGAACAGAACCCAGGGGGGTCATCTGTGGCGGTTTCCGAGCCTGGGAGACCTCTTGTATGAAGCGCGAg  >  2:2240817/1‑140 (MQ=255)
                                                                                                                               cATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCCTTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAg  >  2:1266748/1‑140 (MQ=255)
                                                                                                                               cATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATGTTGAATGAAGCGCGAg  >  1:150139/1‑140 (MQ=255)
                                                                                                                               cATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAg  >  2:900755/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                    
ACTTTATTGGCTTTGATTTTTTTGACTGAAAACTGCAGAGAGCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAG  >  NZ_CP034943/3308287‑3308553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: