Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,308,465 G→T 5.8% Q180K (CAA→AAA)  EO946_RS17110 ← glycosyl hydrolase family 18 protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,308,4650GT 5.8% 430.6 / 11.8 138Q180K (CAA→AAA) EO946_RS17110glycosyl hydrolase family 18 protein
Reads supporting (aligned to +/- strand):  ref base G (78/52);  new base T (4/4);  total (82/56)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 144 total aligned reads displayed.

AGCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGATATTGATACCG  >  NZ_CP034943/3308327‑3308604
                                                                                                                                          |                                                                                                                                           
agCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGa                                                                                                                                            >  2:1260386/1‑140 (MQ=255)
   ttttGATCCAGTTTGTGCTTGCCACTGACCCCGGCTCACCCCAAATGCCGTGTTCATCGTAGGTCATGACTTGTACGAAGTCGGCATATTTGCCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTTatca                                                                                                                                         >  1:1231434/1‑140 (MQ=255)
   ttttGATCCAGTTTGTGCTTGCCACTGACCCCGGCTCACCCCAAATGCCGTGTTCATCGTAGGTCATGACTTGTACGAAGTCGGCATATTTGCCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTTatca                                                                                                                                         >  1:595367/1‑140 (MQ=255)
   ttttGATCCAGTTTGTGCTTGCCACTGACCCCGGCTCACCCCAAATGCCGTGTTCATCGTAGGTCATGACTTGTACGAAGTCGGCATATTTGCCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTTatca                                                                                                                                         >  1:2172943/1‑140 (MQ=255)
     ttGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGatcatc                                                                                                                                       >  2:1190624/1‑140 (MQ=255)
     ttGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCAGCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGatcatc                                                                                                                                       >  2:2029964/1‑140 (MQ=255)
            aGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCtt                                                                                                                                >  2:1511284/1‑140 (MQ=255)
            aGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCtt                                                                                                                                >  2:1353881/1‑140 (MQ=255)
                       gCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCCGCTCCCGTCATCATTTTGATCATCGGCGCTTTTGGCAGGTAc                                                                                                                     >  1:122269/1‑140 (MQ=255)
                       gCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAAc                                                                                                                     <  1:464008/140‑1 (MQ=255)
                       gCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAAc                                                                                                                     <  1:2250483/140‑1 (MQ=255)
                          aCTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGa                                                                                                                  <  2:1816240/140‑1 (MQ=255)
                               tCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCa                                                                                                             <  1:757665/140‑1 (MQ=255)
                               tCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCa                                                                                                             <  1:1604087/140‑1 (MQ=255)
                               tCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCa                                                                                                             <  1:934350/140‑1 (MQ=255)
                               tCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCa                                                                                                             <  2:566421/140‑1 (MQ=255)
                               tCCCGGATCGCCCCAAATGCCGTGTTCATCGAAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCa                                                                                                             <  2:609147/140‑1 (MQ=255)
                                                 gCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCt                                                                                           <  1:1406341/140‑1 (MQ=255)
                                                    ttgtTCATCGTAAGTAATCACCAGCACATAATCCGCAAATTTAACGATTTAAGCATAATCATACCGCCAGCTCCAGTCATCATATTGATCATCGGCGATTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCtttt                                                                                        >  1:440551/2‑140 (MQ=255)
                                                    gtgtTCATCTTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCtttt                                                                                        >  2:1223968/1‑140 (MQ=255)
                                                    gtgtTCATCGTAAGTCATCACCTGCACGTCATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCCTCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCtttt                                                                                        >  2:795675/1‑140 (MQ=255)
                                                    gtgtTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCCTTGTTTTAATATGTTTCtttt                                                                                        >  1:2376764/1‑140 (MQ=255)
                                                    gtgtTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCtttt                                                                                        >  1:1017286/1‑140 (MQ=255)
                                                    gtgtTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCtttt                                                                                        >  1:1078750/1‑140 (MQ=255)
                                                    gtgtTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCtttt                                                                                        >  2:2288749/1‑140 (MQ=255)
                                                    gtgtTCATCGTAAGTCATCACCTGCACGTAAGCCGCATATTTACCGATTTTCGCAGAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAAGCAGTGTGTTAATATGTTTGtttt                                                                                        >  1:1267186/1‑140 (MQ=255)
                                                       ttCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCa                                                                                     <  2:19574/140‑1 (MQ=255)
                                                          aTCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCaga                                                                                   >  2:1510997/1‑139 (MQ=255)
                                                            cGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCAGTGTTTTAATATGTTTCTTTTTCAGAGcc                                                                                >  1:1869057/1‑140 (MQ=255)
                                                                 gTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTga                                                                           >  2:1072349/1‑140 (MQ=255)
                                                                    atcaCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGAc                                                                        >  2:1386243/1‑140 (MQ=255)
                                                                    atcaCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGAc                                                                        >  2:1016897/1‑140 (MQ=255)
                                                                    atcaCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGAc                                                                        >  2:289955/1‑140 (MQ=255)
                                                                    atcaCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGAc                                                                        >  2:962546/1‑140 (MQ=255)
                                                                    atcaCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTAATAGCCTGTGAGAc                                                                        >  2:1523583/1‑140 (MQ=255)
                                                                    atcaCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATAAGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGACTGTGAGAc                                                                        >  2:2329472/1‑140 (MQ=255)
                                                                      caCCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACAt                                                                      >  2:54011/1‑140 (MQ=255)
                                                                            cACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGTACCCATTGTTTTAATATGTTTCTTTTTCAGGGCCTGTGAGACATCTtgaa                                                                >  1:779278/1‑140 (MQ=255)
                                                                            cACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGATCCTGTGAGACATCtttat                                                                >  1:2255066/1‑137 (MQ=255)
                                                                            cACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTtgaa                                                                >  2:1137497/1‑140 (MQ=255)
                                                                                 agtcggCATATTTGCCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTTATCATCGGCGCTTTTGGCCGGAACGGATACCATTGTTTTAATATGTTTCTTATTCAAAGCCTGTGAGACATATTGAATGAAg                                                           <  2:1231434/135‑1 (MQ=255)
                                                                                 aaTCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAg                                                           <  2:2393227/140‑1 (MQ=255)
                                                                                     cGCCTATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg                                                       <  1:1087823/140‑1 (MQ=255)
                                                                                     cGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg                                                       <  1:1497919/140‑1 (MQ=255)
                                                                                     cGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg                                                       <  1:1511284/140‑1 (MQ=255)
                                                                                     cGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg                                                       <  1:1403754/140‑1 (MQ=255)
                                                                                     cGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg                                                       <  1:275271/140‑1 (MQ=255)
                                                                                     cGCATATTTACCGATGTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg                                                       <  1:1352489/140‑1 (MQ=255)
                                                                                     cGCATATTTACCGAGTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGcgcg                                                       <  2:300278/140‑1 (MQ=255)
                                                                                      gCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCAt                                                                                                            <  2:982328/86‑1 (MQ=255)
                                                                                      gCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCAt                                                                                                            >  1:982328/1‑86 (MQ=255)
                                                                                       cATATTTACCGATTTTCGCCTAATCATACGGCCAGCTCCCGTCATCATGTTGATCATCGGCGCTTTTGGCAGGAACAGAACCCAGGGGGGTCATCTGTGGCGGTTTCCGAGCCTGGGAGACCTCTTGTATGAAGCGCGAg                                                     >  2:2240817/1‑140 (MQ=255)
                                                                                       cATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCCTTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAg                                                     >  2:1266748/1‑140 (MQ=255)
                                                                                       cATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATGTTGAATGAAGCGCGAg                                                     >  1:150139/1‑140 (MQ=255)
                                                                                       cATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAg                                                     >  2:900755/1‑140 (MQ=255)
                                                                                                cgatagtCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAAGATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGAt                                            <  2:1842732/134‑1 (MQ=255)
                                                                                                cGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGAACATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGAt                                            <  1:2065449/140‑1 (MQ=255)
                                                                                                  aTTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCg                                          <  2:574398/140‑1 (MQ=255)
                                                                                                  aTTTTCGCATAATCATACGGCCAGCTCCAGTAATCATTTTGATCATCGGCGCTTTTGTCAGGAACAGAAACAATTTTTTTAATATGTTTCTTTTTCAGAGAATGTGATACATCTTTAATGAAGCGCGAGGATTTGTATCg                                          >  2:1525515/1‑140 (MQ=255)
                                                                                                  aTTTTCGCATAATCATACGGCCAGCTCCAGGCATCATTTTGATCATCGCCGCTTTTGGCCTGAACAGTAACCATTGTTTTAATCTGTTACTTTTTCAGAGCCAGTCAGACATCTTCAATGAAGCGCCAGTATTTGGAACg                                          >  2:469582/1‑140 (MQ=255)
                                                                                                    tttCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGGTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGACGCGCGAGGAGTTGGATCGGt                                        >  1:1606400/1‑140 (MQ=255)
                                                                                                    gttCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGACACCGTTGGTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTCTTTGGATCGGt                                        >  2:1608200/2‑140 (MQ=255)
                                                                                                      tCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGtct                                      >  2:779506/1‑140 (MQ=255)
                                                                                                      tCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGtct                                      >  2:1852726/1‑140 (MQ=255)
                                                                                                                ataacgCCAGCTCCATTCATCATTTTGATCATCGTCGCTTTGTGCAGTAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATATTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCa                            <  2:485834/135‑1 (MQ=255)
                                                                                                                aTACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCa                            <  2:353312/140‑1 (MQ=255)
                                                                                                                 tACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGAt                                            >  1:1512781/1‑123 (MQ=255)
                                                                                                                        aGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCCTGGGTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGTATGAAGCGCGAGTAGTTGGATCGGTCTTCTGGAGTCACTGCTTCa                    >  2:1364286/1‑140 (MQ=255)
                                                                                                                        aGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTCATAAGTTTCTTTTTCAGAGCCTGTGAGACATAGTGAATGAATCGCGAGTATTTGGATCGGTCTTCTGGATTAACTTCTTCa                    >  1:839782/1‑140 (MQ=255)
                                                                                                                        aGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGa                                                      <  2:2389971/106‑1 (MQ=255)
                                                                                                                        aGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCg                                          >  1:2171415/1‑118 (MQ=255)
                                                                                                                        aGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCg                                          <  2:2171415/118‑1 (MQ=255)
                                                                                                                        aGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCa                    >  1:1036763/1‑140 (MQ=255)
                                                                                                                        aGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAAGATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCa                    >  1:2133325/1‑140 (MQ=255)
                                                                                                                        aGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCACGAACAGAAACCATGGTTTTAATATGTTTCTTTTTCAGAGCCGGTGATACCTCTTGAATGAAGCGCGAGTATTTGGATAGGTCTTCTGGATTCACTGCTTcc                    >  1:2276327/1‑139 (MQ=255)
                                                                                                                          cTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCaaa                  >  1:326706/1‑140 (MQ=255)
                                                                                                                          cTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCGTCaaa                  >  1:1298020/1‑140 (MQ=255)
                                                                                                                          cTCCAGTCATCATGTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCAGTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGGTCGGTGTTCTGGATTCACTGATTCaaa                  >  1:1624940/1‑140 (MQ=255)
                                                                                                                               gTCATCATTTTGATCATCGGCGCTTTTGGCAGTAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTTAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAACTCGa             >  1:2071336/1‑140 (MQ=255)
                                                                                                                               gTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACCTCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAACTCGa             >  2:1951892/1‑140 (MQ=255)
                                                                                                                               gTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGa             >  2:2027979/1‑140 (MQ=255)
                                                                                                                               gTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGa             >  2:885128/1‑140 (MQ=255)
                                                                                                                               gTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGa             >  1:1977477/1‑140 (MQ=255)
                                                                                                                               gTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGa             >  2:454719/1‑140 (MQ=255)
                                                                                                                               gTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGa             >  2:1037980/1‑140 (MQ=255)
                                                                                                                                tcatcaTTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTGTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGat            <  1:710372/140‑1 (MQ=255)
                                                                                                                                tcatcaTTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTtgaa                                                                <  2:1890855/88‑1 (MQ=255)
                                                                                                                                tcatcaTTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTtgaa                                                                >  1:1890855/1‑88 (MQ=255)
                                                                                                                                    catTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGATATTg        >  1:36517/1‑140 (MQ=255)
                                                                                                                                    catTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGATATTg        >  2:950561/1‑140 (MQ=255)
                                                                                                                                    catTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGATATTg        >  2:796023/1‑140 (MQ=255)
                                                                                                                                     atTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGATATTGa       <  2:2059866/140‑1 (MQ=255)
                                                                                                                                        ttGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCCCTGCTTCAAAATCGATATTGATAc    <  1:546684/140‑1 (MQ=255)
                                                                                                                                        ttGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGATATTGATAc    <  1:2027979/140‑1 (MQ=255)
                                                                                                                                        ttGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGATATTGATAc    <  1:1037980/140‑1 (MQ=255)
                                                                                                                                        ttGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGATATTGATAc    <  2:985966/140‑1 (MQ=255)
                                                                                                                                          gATCATCGGCGTTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGATATTGATACCg  <  1:974581/140‑1 (MQ=255)
                                                                                                                                          gATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGATATTGATACCg  <  2:719249/140‑1 (MQ=255)
                                                                                                                                          gATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGATATTGATACCg  >  2:1155997/1‑140 (MQ=255)
                                                                                                                                          gATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGATATTGATACCg  <  1:707351/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AGCTTTTGATCCAGCTTGTGCTCGCCACTGATCCCGGATCGCCCCAAATGCCGTGTTCATCGTAAGTCATCACCTGCACGTAATCCGCATATTTACCGATTTTCGCATAATCATACGGCCAGCTCCAGTCATCATTTTGATCATCGGCGCTTTTGGCAGGAACAGAAACCATTGTTTTAATATGTTTCTTTTTCAGAGCCTGTGAGACATCTTGAATGAAGCGCGAGTATTTGGATCGGTCTTCTGGATTCACTGCTTCAAAATCGATATTGATACCG  >  NZ_CP034943/3308327‑3308604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: