Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,328,444 A→G 6.2% Q99Q (CAA→CAG lutP → L‑lactate permease LutP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,328,4440AG 6.2% 406.6 / 12.1 130Q99Q (CAA→CAGlutPL‑lactate permease LutP
Reads supporting (aligned to +/- strand):  ref base A (56/65);  new base G (4/4);  total (61/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

Only 100 of 130 total aligned reads displayed.

TGCTGTATTGGTTTATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCCGCGC  >  NZ_CP034943/3328306‑3328580
                                                                                                                                          |                                                                                                                                        
tGCTGTATTGGTTTATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAAt                                                                                                                                         >  2:629845/1‑140 (MQ=255)
 gCTGTATTGGCTTATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACCAGGAGCCGTAGACGGGATTTTGCCGCTCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAAtt                                                                                                                                        <  1:1707518/140‑1 (MQ=255)
     tATTGGTTTATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGat                                                                                                                                    <  2:2326099/140‑1 (MQ=255)
     tATTGGTTTATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGat                                                                                                                                    >  1:1295819/1‑140 (MQ=255)
     tATTGGTTTATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGat                                                                                                                                    >  1:2291501/1‑140 (MQ=255)
     tATTGGTTTATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGat                                                                                                                                    <  2:2037858/140‑1 (MQ=255)
      aTTGGTTTATCGTAGTCCGGCAGAAACAGCTCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGGTGGCTCATTGTGACGTCTGTTTTTTTGTATAATATCACTGTGAAAACGGGACAATTTGata                                                                                                                                   <  1:812000/140‑1 (MQ=255)
      aTTGGTTTATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGata                                                                                                                                   <  1:1333325/140‑1 (MQ=255)
        tGGTTTATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGAtatt                                                                                                                                 <  2:713652/140‑1 (MQ=255)
            ttATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTc                                                                                                                             >  1:1976183/1‑140 (MQ=255)
            ttATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACAAAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTc                                                                                                                             >  1:1796242/1‑140 (MQ=255)
             tATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCg                                                                                                                            <  2:480471/140‑1 (MQ=255)
             tATCGGATGCCGGCAGAAAAAGCGCGGATGTCCGCGACACACGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCg                                                                                                                            <  1:2002697/140‑1 (MQ=255)
               tCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGGTATTATTCGCa                                                                                                                          >  1:26072/1‑140 (MQ=255)
               tCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCa                                                                                                                          >  1:2255978/1‑140 (MQ=255)
                    tGCCGGCGGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGATTGTTGCCGATCGGCTGGATCATTGTGACGTCTGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAGTTTGATATCATTCGCAgctct                                                                                                                     >  2:1877874/1‑140 (MQ=255)
                    tGCCGGCGGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGATTGTTGCCGATCGGCTGGATCATTGTGACGTCTGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAGTTTGATATCATTCGCAgctct                                                                                                                     >  2:44810/1‑140 (MQ=255)
                    tGCCGGCGGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGAGTGTTGCCGATCGGCTGGATCATTGTGACGTCTGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAGTTTGATATCATTCGCAgctct                                                                                                                     >  2:247818/1‑140 (MQ=255)
                    tGCCGGCGGAAAAAGCGCAGATGTCCCCGACACAAGGAGCCGTATCCGGATTGTTGCCGAACGGCGTGATCATTGTGACCTCTGTTTATTTGTATAAAATCACTGTGAAAACTGGACAGTTTGATATCATTCGCAgctct                                                                                                                     >  2:887109/1‑140 (MQ=255)
                    tGCCGGCAGCAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAgttct                                                                                                                     >  1:1427961/1‑140 (MQ=255)
                    tGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAgttct                                                                                                                     >  1:727526/1‑140 (MQ=255)
                    tGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAgttct                                                                                                                     >  1:134775/1‑140 (MQ=255)
                            gAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTtctctc                                                                                                             <  1:1991945/140‑1 (MQ=255)
                              aaaaGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGa                                                                                                           <  1:1390159/140‑1 (MQ=255)
                                     cTGATGTCCGCGACACAAGGCGCCGTATACGGGATTTTGCCGAGCGGCTGGATCATTGTGACGTCAGTTTTTGTGTATAAATTCACTGTGAAAACGGGACAATTTGATAGTATTCGCAGTTCTGTACTCTCGCTTACggt                                                                                                    >  2:2410087/1‑139 (MQ=255)
                                       gaggTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCAGTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATg                                                                                                  <  2:1919326/137‑1 (MQ=255)
                                           tCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccg                                                                                              <  2:28094/140‑1 (MQ=255)
                                           tCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccg                                                                                              <  2:664600/140‑1 (MQ=255)
                                           tCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccg                                                                                              <  1:2409804/140‑1 (MQ=255)
                                           tCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccg                                                                                              <  2:664204/140‑1 (MQ=255)
                                            ccGCGACACAAGGAGCCGTATACGGGTTTTTGCCGATCGGCTGGAACATTGGGACGTCAGTTTTTTTGTATAACATCACTGTGAAAACGGGACAATTTGATATTATACGCAGTTCTGTTCTCTCGATTACGGATGAccgc                                                                                             <  2:1128555/140‑1 (MQ=255)
                                             cgcgACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccgcc                                                                                            <  1:568222/140‑1 (MQ=255)
                                             cgcgACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccgcc                                                                                            <  1:417229/140‑1 (MQ=255)
                                             cgcgACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccgcc                                                                                            <  1:369555/140‑1 (MQ=255)
                                             cgcgACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccgcc                                                                                            <  1:409371/140‑1 (MQ=255)
                                                gACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGc                                                                                         <  1:1360251/140‑1 (MQ=255)
                                                      aGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAgg                                                                                   <  2:2116244/140‑1 (MQ=255)
                                                      aGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAgg                                                                                   <  1:416833/140‑1 (MQ=255)
                                                      aGGAGCCGTATACGGGATTTGGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAgg                                                                                   <  1:1398072/140‑1 (MQ=255)
                                                        gAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGgcg                                                                                 <  2:1761724/140‑1 (MQ=255)
                                                          gCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTGTGTATAAAATCACTGTGAAAACGGGACAAGTTGATCTTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCt                                                                               >  2:1273859/1‑140 (MQ=255)
                                                            cGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTcc                                                                             >  1:1956335/1‑140 (MQ=255)
                                                            cGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTcc                                                                             >  1:1948936/1‑140 (MQ=255)
                                                            agTATACGGGATTTTGCTGATCGGCTGTATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACCATTTGATATTAATCGAACTTCTGTTCTCTCGCTAACGGATGACCGCCGGCTTCAGGCGCTcc                                                                             >  2:1235910/2‑140 (MQ=255)
                                                               ataCGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGa                                                                          <  2:1155141/140‑1 (MQ=255)
                                                                  cGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCg                                                                       <  2:2255978/140‑1 (MQ=255)
                                                                  cGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCg                                                                       <  2:1427961/140‑1 (MQ=255)
                                                                   gggATTTTGCCGATCGGCTGGATCATTGTGACGTCATTTTTTTTGTATAAAATCACTGTGAAAACGGTACAATTTGATATTATTCGCAGTTCTGTTCTCTAGATTACGTATGACCGCCGGCTTCAGCCGCTCCTGATCGc                                                                      >  1:1675534/1‑140 (MQ=255)
                                                                   gggATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAgttct                                                                                                                     >  1:1344181/1‑93 (MQ=255)
                                                                   gggATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAgttct                                                                                                                     <  2:120181/93‑1 (MQ=255)
                                                                   gggATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAgttct                                                                                                                     <  2:1344181/93‑1 (MQ=255)
                                                                   gggATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAgttct                                                                                                                     >  1:120181/1‑93 (MQ=255)
                                                                   gggATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGc                                                                      >  1:1529877/1‑140 (MQ=255)
                                                                     gATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAgg                                                                                   <  2:1735535/125‑1 (MQ=255)
                                                                     gATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCtt                                                                    >  1:1483607/1‑140 (MQ=255)
                                                                     gATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAAGTTGATATTATTCGCAGTTCTGTTCTCTCGATAACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCtt                                                                    >  1:1545711/1‑140 (MQ=255)
                                                                        tttGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGAtatt                                                                                                                                 >  1:1084535/1‑76 (MQ=255)
                                                                        tttGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTtct                                                                 >  2:706663/1‑140 (MQ=255)
                                                                               aTCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGGTATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCgg                                                          <  2:26072/140‑1 (MQ=255)
                                                                               aTCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCgg                                                          <  1:986917/140‑1 (MQ=255)
                                                                                 cGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCgggg                                                        >  1:764750/1‑140 (MQ=255)
                                                                                             tgtgACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAg                                            >  2:965115/1‑140 (MQ=255)
                                                                                             tgtgACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAg                                            >  1:398511/1‑140 (MQ=255)
                                                                                             tgtgACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAg                                            >  2:1007735/1‑140 (MQ=255)
                                                                                             tgtgACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAg                                            >  1:12831/1‑140 (MQ=255)
                                                                                             tgtgACGTCAGTTTGTTTGTATAAAATCACTGTGAAAACGGGAGAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCGTCAGGCGCTCCTGATCGCTTTCTCAGTCGGGGGTTTTTTCGAAg                                            >  1:939455/1‑140 (MQ=255)
                                                                                                 aCGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCg                                                                       >  1:2095522/1‑109 (MQ=255)
                                                                                                 aCGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCg                                                                       <  2:2095522/109‑1 (MQ=255)
                                                                                                 aCGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGc                                        >  1:478000/1‑140 (MQ=255)
                                                                                                    tCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGc                                     <  2:1483607/140‑1 (MQ=255)
                                                                                                     cAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACACTTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGcc                                    >  1:589568/1‑140 (MQ=255)
                                                                                                     cAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGcc                                    <  2:134775/140‑1 (MQ=255)
                                                                                                     cAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGcc                                    >  1:1821068/1‑140 (MQ=255)
                                                                                                     cAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGcc                                    >  1:804876/1‑140 (MQ=255)
                                                                                                     cAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTGTTTAGAAGGAGCTGcc                                    >  1:890507/1‑140 (MQ=255)
                                                                                                      aGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGcc                                    >  1:1530947/1‑139 (MQ=255)
                                                                                                      aGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGcc                                    <  2:1530947/139‑1 (MQ=255)
                                                                                                      aGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCg                                   >  2:873729/1‑140 (MQ=255)
                                                                                                       gTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCgg                                  <  1:30055/140‑1 (MQ=255)
                                                                                                                         aCTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTTCGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGc                <  2:1638792/140‑1 (MQ=255)
                                                                                                                         aCTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGc                <  2:1929990/140‑1 (MQ=255)
                                                                                                                          cTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGc                                     <  1:1851253/118‑1 (MQ=255)
                                                                                                                          cTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGc                                     >  2:1072029/1‑118 (MQ=255)
                                                                                                                          cTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGc                                     <  1:1072029/118‑1 (MQ=255)
                                                                                                                          cTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGc                                     >  2:1851253/1‑118 (MQ=255)
                                                                                                                                 aaCGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTg        <  2:466644/140‑1 (MQ=255)
                                                                                                                                 aaCGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTg        <  2:2254772/140‑1 (MQ=255)
                                                                                                                                    gggACAGTTTGATATCATTCGCAGCTCTGTTCTCTCGATTACTGATGACCGACGGCTTCAAGCTCTCTTGATCGCTTTTTCATTCGGGGCTTTTTTAGAAGGGGCAGCAGGCTTCGGAGCGCCGGTCGCCATTTCTGCcg     <  1:44810/140‑1 (MQ=255)
                                                                                                                                    gggACAGTTTGATATCATTCGCAGCTCTGTTCTCTCGATTACTGATGACCGACGGCTTCAAGCTCTCTTGATCGCTTTTTCATTCGGGGCTTTTTTAGAAGGGGCAGCAGGCTTCGGAGCGCCGGTCGCCATTTCTGCcg     <  1:247818/140‑1 (MQ=255)
                                                                                                                                    gggACAGTTTGATATCATTCGCAGCTCTGTTCTCTCGATTACTGATGACCGACGGCTTCAAGCTCTCTTGATCGCTTTTTCATTCGGGGCTTTTTTAGAAGGGGCAGCAGGCTTCGGAGCGCCGGTCGCCATTTCTGCcg     <  1:1877874/140‑1 (MQ=255)
                                                                                                                                    gggACAGTTTGATATCATTCGCAGCTCTGTTCTCTCGATTACTGATGACCGACGGCTTCAAGCTCTCTTGATCGCTTTTTCATTCGGGGCTTTTTTAGAAGGGGCAGCAGGCTTCGGAGCGCCGGTCGCCATTTCTGCcg     <  1:887109/140‑1 (MQ=255)
                                                                                                                                    gggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTGGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCcg     <  2:721807/140‑1 (MQ=255)
                                                                                                                                    gggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCcg     <  2:2219419/140‑1 (MQ=255)
                                                                                                                                    gggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGATCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCcg     <  2:608826/140‑1 (MQ=255)
                                                                                                                                     ggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCcgc    >  2:2310551/1‑140 (MQ=255)
                                                                                                                                     ggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCcgc    >  1:1703593/1‑140 (MQ=255)
                                                                                                                                     ggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCcgc    >  2:1038371/1‑140 (MQ=255)
                                                                                                                                     ggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCGTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCAGTTCTGCcgc    >  2:2310296/1‑140 (MQ=255)
                                                                                                                                       aCAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCCgcgc  >  1:2329007/1‑140 (MQ=255)
                                                                                                                                       aCAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCCgcgc  >  1:1162535/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                        
TGCTGTATTGGTTTATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCCGCGC  >  NZ_CP034943/3328306‑3328580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: