Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,328,453 T→C 6.6% I102I (ATT→ATC lutP → L‑lactate permease LutP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,328,4530TC 6.6% 383.1 / 11.0 121I102I (ATT→ATClutPL‑lactate permease LutP
Reads supporting (aligned to +/- strand):  ref base T (52/61);  new base C (4/4);  total (56/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

TGGTTTATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCCGCGCTGCTGGT  >  NZ_CP034943/3328314‑3328587
                                                                                                                                           |                                                                                                                                      
tGGTTTATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGAtatt                                                                                                                                        <  2:713652/140‑1 (MQ=255)
    ttATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTc                                                                                                                                    >  1:745459/1‑140 (MQ=255)
    ttATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACAAAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTc                                                                                                                                    >  1:1796242/1‑140 (MQ=255)
     tATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCg                                                                                                                                   <  1:1923595/140‑1 (MQ=255)
     tATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCg                                                                                                                                   <  2:480471/140‑1 (MQ=255)
     tATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCg                                                                                                                                   <  2:1801323/140‑1 (MQ=255)
     tATCGGATGCCGGCAGAAAAAGCGCGGATGTCCGCGACACACGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCg                                                                                                                                   <  1:2002697/140‑1 (MQ=255)
       tCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGGTATTATTCGCa                                                                                                                                 >  1:26072/1‑140 (MQ=255)
       tCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCa                                                                                                                                 >  1:2255978/1‑140 (MQ=255)
            tGCCGGCGGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGATTGTTGCCGATCGGCTGGATCATTGTGACGTCTGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAGTTTGATATCATTCGCAgctct                                                                                                                            >  2:44810/1‑140 (MQ=255)
            tGCCGGCGGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGATTGTTGCCGATCGGCTGGATCATTGTGACGTCTGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAGTTTGATATCATTCGCAgctct                                                                                                                            >  2:1877874/1‑140 (MQ=255)
            tGCCGGCGGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGAGTGTTGCCGATCGGCTGGATCATTGTGACGTCTGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAGTTTGATATCATTCGCAgctct                                                                                                                            >  2:247818/1‑140 (MQ=255)
            tGCCGGCGGAAAAAGCGCAGATGTCCCCGACACAAGGAGCCGTATCCGGATTGTTGCCGAACGGCGTGATCATTGTGACCTCTGTTTATTTGTATAAAATCACTGTGAAAACTGGACAGTTTGATATCATTCGCAgctct                                                                                                                            >  2:887109/1‑140 (MQ=255)
            tGCCGGCAGCAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAgttct                                                                                                                            >  1:1427961/1‑140 (MQ=255)
            tGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAgttct                                                                                                                            >  1:134775/1‑140 (MQ=255)
                 gCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAgttctgttct                                                                                                                       >  2:1360251/1‑140 (MQ=255)
                  cAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTtctc                                                                                                                      <  1:243119/140‑1 (MQ=255)
                    gAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTtctctc                                                                                                                    <  1:1991945/140‑1 (MQ=255)
                      aaaaGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGa                                                                                                                  <  1:1390159/140‑1 (MQ=255)
                             cTGATGTCCGCGACACAAGGCGCCGTATACGGGATTTTGCCGAGCGGCTGGATCATTGTGACGTCAGTTTTTGTGTATAAATTCACTGTGAAAACGGGACAATTTGATAGTATTCGCAGTTCTGTACTCTCGCTTACggt                                                                                                           >  2:2410087/1‑139 (MQ=255)
                               gaggTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCAGTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATg                                                                                                         <  2:1919326/137‑1 (MQ=255)
                                   tCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccg                                                                                                     <  1:2409804/140‑1 (MQ=255)
                                   tCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccg                                                                                                     <  2:28094/140‑1 (MQ=255)
                                   tCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccg                                                                                                     <  2:664204/140‑1 (MQ=255)
                                   tCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccg                                                                                                     <  2:494618/140‑1 (MQ=255)
                                    ccGCGACACAAGGAGCCGTATACGGGTTTTTGCCGATCGGCTGGAACATTGGGACGTCAGTTTTTTTGTATAACATCACTGTGAAAACGGGACAATTTGATATTATACGCAGTTCTGTTCTCTCGATTACGGATGAccgc                                                                                                    <  2:1128555/140‑1 (MQ=255)
                                     cgcgACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccgcc                                                                                                   <  1:417229/140‑1 (MQ=255)
                                     cgcgACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccgcc                                                                                                   <  1:568222/140‑1 (MQ=255)
                                     cgcgACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGAccgcc                                                                                                   <  1:409371/140‑1 (MQ=255)
                                        gACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGc                                                                                                <  1:1360251/140‑1 (MQ=255)
                                              aGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAgg                                                                                          <  1:416833/140‑1 (MQ=255)
                                              aGGAGCCGTATACGGGATTTGGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAgg                                                                                          <  1:1398072/140‑1 (MQ=255)
                                                gAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGgcg                                                                                        <  1:1580311/140‑1 (MQ=255)
                                                gAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGgcg                                                                                        <  2:1761724/140‑1 (MQ=255)
                                                  gCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACCATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCt                                                                                      >  2:81609/1‑140 (MQ=255)
                                                  gCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTGTGTATAAAATCACTGTGAAAACGGGACAAGTTGATCTTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCt                                                                                      >  2:1273859/1‑140 (MQ=255)
                                                    cGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACTGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTcc                                                                                    >  2:405234/1‑140 (MQ=255)
                                                    cGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTcc                                                                                    >  1:1948936/1‑140 (MQ=255)
                                                    cGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTcc                                                                                    >  1:1956335/1‑140 (MQ=255)
                                                    agTATACGGGATTTTGCTGATCGGCTGTATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACCATTTGATATTAATCGAACTTCTGTTCTCTCGCTAACGGATGACCGCCGGCTTCAGGCGCTcc                                                                                    >  2:1235910/2‑140 (MQ=255)
                                                       ataCGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGa                                                                                 <  2:1155141/140‑1 (MQ=255)
                                                        taCGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGGTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGAt                                                                                <  2:695173/140‑1 (MQ=255)
                                                          cGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCg                                                                              <  2:2255978/140‑1 (MQ=255)
                                                          cGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCg                                                                              <  2:1427961/140‑1 (MQ=255)
                                                           gggATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAgttct                                                                                                                            <  2:1344181/93‑1 (MQ=255)
                                                           gggATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAgttct                                                                                                                            >  1:1344181/1‑93 (MQ=255)
                                                           gggATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAgttct                                                                                                                            >  1:120181/1‑93 (MQ=255)
                                                           gggATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGc                                                                             >  1:1529877/1‑140 (MQ=255)
                                                             gATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAgg                                                                                          >  1:1735535/1‑125 (MQ=255)
                                                             gATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAgg                                                                                          <  2:1735535/125‑1 (MQ=255)
                                                             gATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCtt                                                                           >  1:1483607/1‑140 (MQ=255)
                                                             gATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAAGTTGATATTATTCGCAGTTCTGTTCTCTCGATAACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCtt                                                                           >  1:1545711/1‑140 (MQ=255)
                                                                tttGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGAtatt                                                                                                                                        >  1:1084535/1‑76 (MQ=255)
                                                                tttGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGAtatt                                                                                                                                        <  2:1084535/76‑1 (MQ=255)
                                                                tttGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTtct                                                                        >  1:533725/1‑140 (MQ=255)
                                                                tttGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTtct                                                                        >  2:706663/1‑140 (MQ=255)
                                                                       aTCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCgg                                                                 <  1:342503/140‑1 (MQ=255)
                                                                       aTCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCgg                                                                 <  1:986917/140‑1 (MQ=255)
                                                                         cGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCgggg                                                               >  1:764750/1‑140 (MQ=255)
                                                                                     tgtgACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAg                                                   >  2:965115/1‑140 (MQ=255)
                                                                                     tgtgACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAg                                                   >  2:1007735/1‑140 (MQ=255)
                                                                                     tgtgACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAg                                                   >  1:993672/1‑140 (MQ=255)
                                                                                     tgtgACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAg                                                   >  1:398511/1‑140 (MQ=255)
                                                                                     tgtgACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAg                                                   >  1:12831/1‑140 (MQ=255)
                                                                                     tgtgACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAg                                                   >  1:857684/1‑140 (MQ=255)
                                                                                     tgtgACGTCAGTTTGTTTGTATAAAATCACTGTGAAAACGGGAGAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCGTCAGGCGCTCCTGATCGCTTTCTCAGTCGGGGGTTTTTTCGAAg                                                   >  1:939455/1‑140 (MQ=255)
                                                                                         aCGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCg                                                                              >  1:2095522/1‑109 (MQ=255)
                                                                                         aCGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCg                                                                              <  2:2095522/109‑1 (MQ=255)
                                                                                         aCGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGc                                               >  1:478000/1‑140 (MQ=255)
                                                                                            tCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGc                                            <  2:1483607/140‑1 (MQ=255)
                                                                                             cAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACACTTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGcc                                           >  1:589568/1‑140 (MQ=255)
                                                                                             cAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGcc                                           >  1:1821068/1‑140 (MQ=255)
                                                                                             cAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGcc                                           >  1:804876/1‑140 (MQ=255)
                                                                                             cAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTGTTTAGAAGGAGCTGcc                                           >  1:890507/1‑140 (MQ=255)
                                                                                              aGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGcc                                           >  1:1530947/1‑139 (MQ=255)
                                                                                              aGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGcc                                           <  2:1530947/139‑1 (MQ=255)
                                                                                              aGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCg                                          >  2:873729/1‑140 (MQ=255)
                                                                                               gTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCgg                                         <  1:30055/140‑1 (MQ=255)
                                                                                                       gTATAAAATCACTGTGCAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAg                                 <  1:1989578/140‑1 (MQ=255)
                                                                                                                 aCTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTTCGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGc                       <  2:1638792/140‑1 (MQ=255)
                                                                                                                 aCTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGc                       <  2:1929990/140‑1 (MQ=255)
                                                                                                                  cTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGc                                            >  2:1072029/1‑118 (MQ=255)
                                                                                                                  cTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGc                                            <  1:1072029/118‑1 (MQ=255)
                                                                                                                         aaCGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTg               <  2:466644/140‑1 (MQ=255)
                                                                                                                         aaCGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTg               <  2:2254772/140‑1 (MQ=255)
                                                                                                                            gggACAGTTTGATATCATTCGCAGCTCTGTTCTCTCGATTACTGATGACCGACGGCTTCAAGCTCTCTTGATCGCTTTTTCATTCGGGGCTTTTTTAGAAGGGGCAGCAGGCTTCGGAGCGCCGGTCGCCATTTCTGCcg            <  1:887109/140‑1 (MQ=255)
                                                                                                                            gggACAGTTTGATATCATTCGCAGCTCTGTTCTCTCGATTACTGATGACCGACGGCTTCAAGCTCTCTTGATCGCTTTTTCATTCGGGGCTTTTTTAGAAGGGGCAGCAGGCTTCGGAGCGCCGGTCGCCATTTCTGCcg            <  1:1877874/140‑1 (MQ=255)
                                                                                                                            gggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCcg            <  2:1206054/140‑1 (MQ=255)
                                                                                                                            gggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCcg            <  2:2116719/140‑1 (MQ=255)
                                                                                                                            gggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCcg            <  2:2219419/140‑1 (MQ=255)
                                                                                                                            gggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGATCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCcg            <  2:608826/140‑1 (MQ=255)
                                                                                                                             ggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCcgc           >  1:1703593/1‑140 (MQ=255)
                                                                                                                             ggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCcgc           >  2:2310551/1‑140 (MQ=255)
                                                                                                                             ggACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCGTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCAGTTCTGCcgc           >  2:2310296/1‑140 (MQ=255)
                                                                                                                               aCAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCCgcgc         >  1:2329007/1‑140 (MQ=255)
                                                                                                                               aCAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCCgcgc         >  1:1162535/1‑140 (MQ=255)
                                                                                                                                      gagattattCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGGCATTTCTGCCGCGCTGCTGGt  <  2:692394/137‑1 (MQ=255)
                                                                                                                                      gATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCCGCGCTGCTGGt  <  1:249215/140‑1 (MQ=255)
                                                                                                                                      gATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCCGCGCTGCTGGt  <  1:940278/140‑1 (MQ=255)
                                                                                                                                      gATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCCGCGCTGCTGGt  <  1:965115/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
TGGTTTATCGGATGCCGGCAGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGGATTTTGCCGATCGGCTGGATCATTGTGACGTCAGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAATTTGATATTATTCGCAGTTCTGTTCTCTCGATTACGGATGACCGCCGGCTTCAGGCGCTCCTGATCGCTTTCTCATTCGGGGCTTTTTTAGAAGGAGCTGCCGGCTTCGGAGCGCCGGTCGCCATTTCTGCCGCGCTGCTGGT  >  NZ_CP034943/3328314‑3328587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: