Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,340,444 G→A 6.1% L66L (CTG→TTG)  epsG ← biofilm exopolysaccharide biosynthesis protein EpsG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,340,4440GA 6.1% 292.9 / 7.2 98L66L (CTG→TTG) epsGbiofilm exopolysaccharide biosynthesis protein EpsG
Reads supporting (aligned to +/- strand):  ref base G (51/40);  new base A (4/2);  total (55/43)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.18e-01

Only 100 of 109 total aligned reads displayed.

ATATACATGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCG  >  NZ_CP034943/3340305‑3340577
                                                                                                                                           |                                                                                                                                     
atataCATGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTAcag                                                                                                                                       <  1:1263219/140‑1 (MQ=255)
atataCATGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTAcag                                                                                                                                       <  1:761749/140‑1 (MQ=255)
  ataCATGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTAcagca                                                                                                                                     <  1:1086244/140‑1 (MQ=255)
  ataCATGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTAcagca                                                                                                                                     <  1:2045765/140‑1 (MQ=255)
  ataCATGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTAcagca                                                                                                                                     <  2:363853/140‑1 (MQ=255)
  ataCATGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTAcagca                                                                                                                                     <  2:2070961/140‑1 (MQ=255)
  ataCATGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGAGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTAcagca                                                                                                                                     <  2:489636/140‑1 (MQ=255)
  ataCAGGATGTGCGGAGCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAAGAGCGGCCACACATTTTGATAATCGCCCGCCAATTCGTAcagca                                                                                                                                     <  2:2249393/140‑1 (MQ=255)
      aTGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACAAATTTTGATAATCGCCCGCCAATTCGTACAGCAgtgt                                                                                                                                 >  1:1219207/1‑140 (MQ=255)
       tGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAgtgtg                                                                                                                                >  1:635241/1‑140 (MQ=255)
       tGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTGTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAgtgtg                                                                                                                                >  1:2192178/1‑140 (MQ=255)
        gATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAgtgtgt                                                                                                                               >  1:478271/1‑140 (MQ=255)
          tttGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTAc                                                                                                                             <  2:1656783/140‑1 (MQ=255)
           ttGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTAcagca                                                                                                                                     >  2:379372/1‑131 (MQ=255)
           ttGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTAcagca                                                                                                                                     <  1:379372/131‑1 (MQ=255)
           ttGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACg                                                                                                                            >  2:240951/1‑140 (MQ=255)
              cGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGttt                                                                                                                         >  1:224790/1‑140 (MQ=255)
                 aTCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGaa                                                                                                                      <  2:1034168/140‑1 (MQ=255)
                 aTCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGaa                                                                                                                      <  2:1255918/140‑1 (MQ=255)
                   cTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAACCCCGGATCTGTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGaaaa                                                                                                                    >  1:617719/1‑140 (MQ=255)
                     tCCGTGAGGAACTTCATCAGCCAAAGGAGGGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAAGATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATc                                                                                                                  <  2:845766/140‑1 (MQ=255)
                          gatgaAATTCCTCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGACACCGAATTTCTGCGAGACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCGGTGc                                                                                                             <  2:28517/140‑1 (MQ=255)
                             gaAATTCATCAGCCAACGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          <  1:811310/140‑1 (MQ=255)
                             gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACTTTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          <  1:2009564/140‑1 (MQ=255)
                             gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          <  1:468170/140‑1 (MQ=255)
                             gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          <  1:547902/140‑1 (MQ=255)
                             gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          <  1:2079519/140‑1 (MQ=255)
                             gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          <  2:1161524/140‑1 (MQ=255)
                             gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          <  1:1573966/140‑1 (MQ=255)
                             gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          <  1:1521612/140‑1 (MQ=255)
                             gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          <  1:1972873/140‑1 (MQ=255)
                             gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          <  1:1474934/140‑1 (MQ=255)
                             gaAATTCATCAGCAAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          <  1:1341423/140‑1 (MQ=255)
                                aTTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGActc                                                                                                       >  1:2127252/1‑140 (MQ=255)
                                aTTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGActc                                                                                                       >  1:1169106/1‑140 (MQ=255)
                                         ccAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGaa                                                                                              >  1:1705858/1‑140 (MQ=255)
                                                 gTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGac                                                                                      <  2:1090257/140‑1 (MQ=255)
                                                  tGCGGTAACGCCCGGGGCTGTCGCCGTTTTTGCTGTGCCGAAACCCAAAATCTGCCACACAGTTTGATAATCGCCCGCCAATTCGTACAGAAGTGTGTACGTTTGAAAATCCGTTCCGACTCTGTAACGAAGAACCGaca                                                                                     >  1:2400411/1‑140 (MQ=255)
                                                  tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                     >  2:5362/1‑140 (MQ=255)
                                                  tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                     >  2:1722298/1‑140 (MQ=255)
                                                  tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                     >  1:1367134/1‑140 (MQ=255)
                                                  tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                     >  1:263145/1‑140 (MQ=255)
                                                  tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                     >  1:1447306/1‑140 (MQ=255)
                                                  tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                     >  2:1697745/1‑140 (MQ=255)
                                                  tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                     >  1:790686/1‑140 (MQ=255)
                                                  tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                     >  1:2360903/1‑140 (MQ=255)
                                                  tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                     >  1:1603153/1‑140 (MQ=255)
                                                  tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                     >  1:1644065/1‑140 (MQ=255)
                                                  tGCGGTAAAGCCCGGGTCTGTCGCCCTGTTTGCTGTGCCGCAACCGAATAGCTGCCACACAGTTTGATAAACGCCCGCCAATTCGTGCAGCGGTGTGTACGTGTGTAACTCCGTGCCGACTCTGTAACGAAGACCCGacc                                                                                     >  1:2277626/1‑139 (MQ=255)
                                                  tGCGGTAAACCCCGGATCTGTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGaca                                                                                     >  2:1770178/1‑140 (MQ=255)
                                                  tGCGGTAAACCCCGGATCTGTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGaca                                                                                     >  1:2391880/1‑140 (MQ=255)
                                                   gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACCGTTTGATAATCGCCCGCCAATTCGTACCGCAGGGTGTGCGTTTGACAATCCGTGCCGCCTCTGGAACGAAGACCCGaccc                                                                                    >  1:2123580/1‑138 (MQ=255)
                                                   gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGccac                                                                                    >  1:1312284/1‑140 (MQ=255)
                                                   gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                    <  2:236253/140‑1 (MQ=255)
                                                   gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                    >  1:1519390/1‑140 (MQ=255)
                                                   gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                    >  1:23063/1‑140 (MQ=255)
                                                   gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGGGTACGGTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaccc                                                                                    >  1:1469310/1‑138 (MQ=255)
                                                      gTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAg                                                                                 <  1:1722298/140‑1 (MQ=255)
                                                       tAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGc                                                                                >  1:655160/1‑140 (MQ=255)
                                                        aaaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGACTATCTGCCACACATTTTGATAATCGGCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGca                                                                               <  1:728284/140‑1 (MQ=255)
                                                        aaaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGca                                                                               <  1:1749455/140‑1 (MQ=255)
                                                         aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          <  1:2158781/112‑1 (MQ=255)
                                                         aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          >  2:2158781/1‑112 (MQ=255)
                                                         aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcac                                                                              >  1:2411336/1‑140 (MQ=255)
                                                         aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcac                                                                              >  1:639124/1‑140 (MQ=255)
                                                            cccGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGCAACGAAGACCCGACACGAGCACAAg                                                                           >  1:324233/1‑140 (MQ=255)
                                                                 gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                      >  1:2971/1‑140 (MQ=255)
                                                                 gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATAGCCCGCCCATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                      >  1:1950217/1‑140 (MQ=255)
                                                                     gTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGaagcgag                                                                  <  2:2391880/140‑1 (MQ=255)
                                                                      tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGACGACCCGACACGAGCACAAGTGaagcaagc                                                                 >  2:1988236/1‑140 (MQ=255)
                                                                      tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                 >  2:2265377/1‑140 (MQ=255)
                                                                      tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                 >  2:920246/1‑140 (MQ=255)
                                                                      tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGACCACAAGTGaagcaagc                                                                 >  1:2380248/1‑140 (MQ=255)
                                                                      tCGCCGTTTTTGCTGTGCCGAAACCGAATATATGCCACACATTTTGATAAACGCCCGCCAAATCGTACAGCAGTGTGTACGGTTGAAAATACGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                 >  1:969016/1‑140 (MQ=255)
                                                                       cGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCg                                                                <  1:240951/140‑1 (MQ=255)
                                                                           gTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAAt                                                            >  1:2185892/1‑140 (MQ=255)
                                                                                   tgtgCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCa                                                    <  1:1155452/140‑1 (MQ=255)
                                                                                   tgtgCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGAAGCGAGCGGAATCCAGATCa                                                    <  2:617719/140‑1 (MQ=255)
                                                                                    ttgCCGAAACCGAAAATCTGCCACACATTTTGATAATCGCCCGCCACTTCGTACAGCACTGGGTACGTTTGCAAATCCGTGCCGACTCTGTAACGAAGACCCGACCCGAGCACCAGAGACGCCAGCGGAATCCCAATCac                                                   >  2:1999423/2‑139 (MQ=255)
                                                                                       ccGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          <  2:1969305/82‑1 (MQ=255)
                                                                                       ccGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                          >  1:1969305/1‑82 (MQ=255)
                                                                                            aCCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAt                                           <  2:2430351/140‑1 (MQ=255)
                                                                                             ccGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAtt                                          <  2:1644065/140‑1 (MQ=255)
                                                                                             ccGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAtt                                          <  2:1447306/140‑1 (MQ=255)
                                                                                                   atCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCg                                    <  1:1494536/140‑1 (MQ=255)
                                                                                                   atCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCg                                    <  1:1660032/140‑1 (MQ=255)
                                                                                                      tGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATa                                 >  1:1089110/1‑140 (MQ=255)
                                                                                                       gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                >  2:2360961/1‑140 (MQ=255)
                                                                                                       gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                >  1:399751/1‑140 (MQ=255)
                                                                                                       gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                >  2:2264173/1‑140 (MQ=255)
                                                                                                               ttttGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGc                        <  1:1726619/140‑1 (MQ=255)
                                                                                                               ttttGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGATTGAAAATCCGGGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGc                        <  2:554742/140‑1 (MQ=255)
                                                                                                                    aTAATCGCCCGCCAATTCGTACATCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTg                   <  2:2192178/140‑1 (MQ=255)
                                                                                                                    aTAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTg                   <  2:635241/140‑1 (MQ=255)
                                                                                                                      aaTCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGaa                 <  2:655160/140‑1 (MQ=255)
                                                                                                                               ccAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACgg        <  2:2360903/140‑1 (MQ=255)
                                                                                                                               ccAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACgg        <  2:790686/140‑1 (MQ=255)
                                                                                                                                   ttCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGgccgc    >  1:236234/1‑140 (MQ=255)
                                                                                                                                     cGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGccgccg  <  2:1071910/140‑1 (MQ=255)
                                                                                                                                     cGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGccgccg  <  2:2007136/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                     
ATATACATGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCG  >  NZ_CP034943/3340305‑3340577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: