Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,340,448 T→C 6.4% T64T (ACA→ACG epsG ← biofilm exopolysaccharide biosynthesis protein EpsG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,340,4480TC 6.4% 291.2 / 7.8 94T64T (ACA→ACGepsGbiofilm exopolysaccharide biosynthesis protein EpsG
Reads supporting (aligned to +/- strand):  ref base T (46/41);  new base C (4/2);  total (51/43)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01

Only 100 of 108 total aligned reads displayed.

ATGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGG  >  NZ_CP034943/3340311‑3340587
                                                                                                                                         |                                                                                                                                           
aTGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACAAATTTTGATAATCGCCCGCCAATTCGTACAGCAgtgt                                                                                                                                           >  1:1219207/1‑140 (MQ=255)
aTGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAAACGAATATCTGCCACACAATTTGATAATCGCCCGCCAATTCGTACAGCAgtgt                                                                                                                                           >  1:1490860/1‑140 (MQ=255)
 tGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAgtgtg                                                                                                                                          >  1:635241/1‑140 (MQ=255)
 tGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTGTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAgtgtg                                                                                                                                          >  1:2192178/1‑140 (MQ=255)
  gATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAgtgtgt                                                                                                                                         >  1:478271/1‑140 (MQ=255)
    tttGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTAc                                                                                                                                       <  2:1656783/140‑1 (MQ=255)
        cGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGttt                                                                                                                                   >  1:224790/1‑140 (MQ=255)
           aTCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGaa                                                                                                                                <  2:1034168/140‑1 (MQ=255)
           aTCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGaa                                                                                                                                <  2:1255918/140‑1 (MQ=255)
             cTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAACCCCGGATCTGTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGaaaa                                                                                                                              >  1:617719/1‑140 (MQ=255)
               tCCGTGAGGAACTTCATCAGCCAAAGGAGGGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAAGATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATc                                                                                                                            <  2:845766/140‑1 (MQ=255)
                    gatgaAATTCCTCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGACACCGAATTTCTGCGAGACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCGGTGc                                                                                                                       <  2:28517/140‑1 (MQ=255)
                       gaAATTCATCAGCCAACGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                    <  1:811310/140‑1 (MQ=255)
                       gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACTTTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                    <  1:2009564/140‑1 (MQ=255)
                       gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                    <  1:1474934/140‑1 (MQ=255)
                       gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                    <  1:468170/140‑1 (MQ=255)
                       gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                    <  1:2079519/140‑1 (MQ=255)
                       gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                    <  2:1161524/140‑1 (MQ=255)
                       gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                    <  1:1573966/140‑1 (MQ=255)
                       gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                    <  1:1521612/140‑1 (MQ=255)
                       gaAATTCATCAGCAAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                    <  1:1341423/140‑1 (MQ=255)
                          aTTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGActc                                                                                                                 >  1:1169106/1‑140 (MQ=255)
                          aTTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGActc                                                                                                                 >  1:2127252/1‑140 (MQ=255)
                                   ccAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGaa                                                                                                        >  1:1705858/1‑140 (MQ=255)
                                   ccAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGaa                                                                                                        <  1:674746/140‑1 (MQ=255)
                                            tGCGGTAACGCCCGGGGCTGTCGCCGTTTTTGCTGTGCCGAAACCCAAAATCTGCCACACAGTTTGATAATCGCCCGCCAATTCGTACAGAAGTGTGTACGTTTGAAAATCCGTTCCGACTCTGTAACGAAGAACCGaca                                                                                               >  1:2400411/1‑140 (MQ=255)
                                            tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                               >  1:1644065/1‑140 (MQ=255)
                                            tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                               >  1:2360903/1‑140 (MQ=255)
                                            tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                               >  2:1722298/1‑140 (MQ=255)
                                            tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                               >  1:1447306/1‑140 (MQ=255)
                                            tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                               >  1:263145/1‑140 (MQ=255)
                                            tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                               >  2:1697745/1‑140 (MQ=255)
                                            tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                               >  1:1367134/1‑140 (MQ=255)
                                            tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                               >  1:790686/1‑140 (MQ=255)
                                            tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                               >  1:1603153/1‑140 (MQ=255)
                                            tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                               >  2:5362/1‑140 (MQ=255)
                                            tGCGGTAAAGCCCGGGTCTGTCGCCCTGTTTGCTGTGCCGCAACCGAATAGCTGCCACACAGTTTGATAAACGCCCGCCAATTCGTGCAGCGGTGTGTACGTGTGTAACTCCGTGCCGACTCTGTAACGAAGACCCGacc                                                                                               >  1:2277626/1‑139 (MQ=255)
                                            tGCGGTAAACCCCGGATCTGTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGaca                                                                                               >  2:309448/1‑140 (MQ=255)
                                            tGCGGTAAACCCCGGATCTGTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGaca                                                                                               >  1:2391880/1‑140 (MQ=255)
                                            tGCGGTAAACCCCGGATCTGTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGaca                                                                                               >  2:1770178/1‑140 (MQ=255)
                                             gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACCGTTTGATAATCGCCCGCCAATTCGTACCGCAGGGTGTGCGTTTGACAATCCGTGCCGCCTCTGGAACGAAGACCCGaccc                                                                                              >  1:2123580/1‑138 (MQ=255)
                                             gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGccac                                                                                              >  1:1312284/1‑140 (MQ=255)
                                             gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                              >  1:23063/1‑140 (MQ=255)
                                             gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                              <  2:236253/140‑1 (MQ=255)
                                             gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGGGTACGGTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaccc                                                                                              >  1:1469310/1‑138 (MQ=255)
                                                gTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAg                                                                                           <  1:1722298/140‑1 (MQ=255)
                                                 tAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGc                                                                                          >  1:655160/1‑140 (MQ=255)
                                                  aaaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGACTATCTGCCACACATTTTGATAATCGGCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGca                                                                                         <  1:728284/140‑1 (MQ=255)
                                                  aaaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGca                                                                                         <  1:1749455/140‑1 (MQ=255)
                                                   aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                    <  1:2158781/112‑1 (MQ=255)
                                                   aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                    >  2:2158781/1‑112 (MQ=255)
                                                   aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcac                                                                                        >  1:639124/1‑140 (MQ=255)
                                                   aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcac                                                                                        >  1:2411336/1‑140 (MQ=255)
                                                      cccGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAg                                                                                     <  2:1543671/140‑1 (MQ=255)
                                                      cccGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGCAACGAAGACCCGACACGAGCACAAg                                                                                     >  1:324233/1‑140 (MQ=255)
                                                           gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                >  1:2971/1‑140 (MQ=255)
                                                           gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                >  1:1949896/1‑140 (MQ=255)
                                                           gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                >  1:2870/1‑140 (MQ=255)
                                                           gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATAGCCCGCCCATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                >  1:1950217/1‑140 (MQ=255)
                                                               gTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGaagcgag                                                                            <  2:2391880/140‑1 (MQ=255)
                                                                tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGACGACCCGACACGAGCACAAGTGaagcaagc                                                                           >  2:1988236/1‑140 (MQ=255)
                                                                tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                           >  2:2265377/1‑140 (MQ=255)
                                                                tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                           >  2:920246/1‑140 (MQ=255)
                                                                tCGCCGTTTTTGCTGTGCCGAAACCGAATATATGCCACACATTTTGATAAACGCCCGCCAAATCGTACAGCAGTGTGTACGGTTGAAAATACGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                           >  1:969016/1‑140 (MQ=255)
                                                                 cGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCg                                                                          <  1:240951/140‑1 (MQ=255)
                                                                     gTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAAt                                                                      >  1:2185892/1‑140 (MQ=255)
                                                                             tgtgCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCa                                                              <  1:1155452/140‑1 (MQ=255)
                                                                             tgtgCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGAAGCGAGCGGAATCCAGATCa                                                              <  2:617719/140‑1 (MQ=255)
                                                                              ttgCCGAAACCGAAAATCTGCCACACATTTTGATAATCGCCCGCCACTTCGTACAGCACTGGGTACGTTTGCAAATCCGTGCCGACTCTGTAACGAAGACCCGACCCGAGCACCAGAGACGCCAGCGGAATCCCAATCac                                                             >  2:1999423/2‑139 (MQ=255)
                                                                                 ccGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                    <  2:1969305/82‑1 (MQ=255)
                                                                                 ccGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                    >  1:1969305/1‑82 (MQ=255)
                                                                                 ccGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACACGTGAAGCAAGCGGAATCCAAATCAAAAg                                                          >  1:2234779/1‑140 (MQ=255)
                                                                                      aCCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAt                                                     <  2:2430351/140‑1 (MQ=255)
                                                                                       ccGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAtt                                                    <  2:1644065/140‑1 (MQ=255)
                                                                                       ccGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAtt                                                    <  2:1447306/140‑1 (MQ=255)
                                                                                             atCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCg                                              <  1:1660032/140‑1 (MQ=255)
                                                                                             atCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCg                                              <  1:1494536/140‑1 (MQ=255)
                                                                                                tGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATa                                           >  1:1089110/1‑140 (MQ=255)
                                                                                                 gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                          >  2:2360961/1‑140 (MQ=255)
                                                                                                 gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                          >  2:2264173/1‑140 (MQ=255)
                                                                                                 gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                          >  1:399751/1‑140 (MQ=255)
                                                                                                         ttttGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGc                                  <  1:1726619/140‑1 (MQ=255)
                                                                                                         ttttGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGATTGAAAATCCGGGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGc                                  <  2:554742/140‑1 (MQ=255)
                                                                                                              aTAATCGCCCGCCAATTCGTACATCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTg                             <  2:2192178/140‑1 (MQ=255)
                                                                                                              aTAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTg                             <  2:635241/140‑1 (MQ=255)
                                                                                                                aaTCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGaa                           <  2:655160/140‑1 (MQ=255)
                                                                                                                         ccAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACgg                  <  2:790686/140‑1 (MQ=255)
                                                                                                                         ccAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACgg                  <  2:2360903/140‑1 (MQ=255)
                                                                                                                             ttCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGgccgc              >  1:236234/1‑140 (MQ=255)
                                                                                                                               cGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGccgccg            <  2:1071910/140‑1 (MQ=255)
                                                                                                                               cGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGccgccg            <  2:2007136/140‑1 (MQ=255)
                                                                                                                                      cagTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACAttt     >  2:1705941/1‑140 (MQ=255)
                                                                                                                                        gtgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTg   >  2:1486958/1‑140 (MQ=255)
                                                                                                                                         tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg  >  2:426862/1‑140 (MQ=255)
                                                                                                                                         tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg  >  1:770226/1‑140 (MQ=255)
                                                                                                                                         tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg  >  1:930549/1‑140 (MQ=255)
                                                                                                                                         tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg  >  1:2389168/1‑140 (MQ=255)
                                                                                                                                         tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg  >  2:2271337/1‑140 (MQ=255)
                                                                                                                                         tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg  >  1:1005835/1‑140 (MQ=255)
                                                                                                                                         tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAGTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg  <  2:1705858/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
ATGATTTGCGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGG  >  NZ_CP034943/3340311‑3340587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: