BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	132,186	A→G	94.7%	D560G (GAC→GGC)	fusA →	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	132,186	0	A	G	94.7%	727.4 / 19.2	226	D560G (GAC→GGC)	fusA	elongation factor G
Reads supporting (aligned to +/- strand): ref base A (6/6); new base G (128/85); total (134/92)
Fisher's exact test for biased strand distribution p-value = 5.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

Only 100 of 226 total aligned reads displayed.

AACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGAAA  >  NZ_CP034943/132050‑132322

aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCAt                                                                                                                                       >  1:2197108/1‑140 (MQ=255)

aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCAt                                                                                                                                       >  1:1630924/1‑140 (MQ=255)

             cttgcttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTATAGCAAAACtt                                                                                                                          >  2:1668528/1‑140 (MQ=255)

             cttgcttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACtt                                                                                                                          >  2:1641437/1‑140 (MQ=255)

             cttgcttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACtt                                                                                                                          >  2:558247/1‑140 (MQ=255)

               tgcttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACtttt                                                                                                                        >  2:178992/1‑140 (MQ=255)

               tgcttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACtttt                                                                                                                        >  1:2187939/1‑140 (MQ=255)

                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTGGa                                                                                                                     <  1:2192680/140‑1 (MQ=255)

                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     <  2:229829/140‑1 (MQ=255)

                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     <  2:1630115/140‑1 (MQ=255)

                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     <  1:376642/140‑1 (MQ=255)

                  tttgaatgtgacAATGCGATTGTCGGTGGGGTAGGTCCACGTGAATACAGCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACGTGAACATGGTGTACTGGCAGGCGTCCGGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     <  2:1957364/140‑1 (MQ=255)

                   ttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGAt                                                                                                                    <  2:402603/140‑1 (MQ=255)

                      aatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGt                                                                                                                 <  1:1165100/140‑1 (MQ=255)

                      aatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGt                                                                                                                 <  1:396961/140‑1 (MQ=255)

                           gaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTa                                                                                                            >  2:1111873/1‑140 (MQ=255)

                             aaaTGCGATTGTCGGTGGGTTAGGTCCACGTGAATACATCCGAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTAcc                                                                                                          <  2:2090383/140‑1 (MQ=255)

                                                    ttCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATg                                                                                   <  1:317306/140‑1 (MQ=255)

                                                      ccACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGa                                                                                                                     >  1:670370/1‑104 (MQ=255)

                                                         cGTGAATACATCCCAGCTGTTCAAGCAGTTCTGGAAGATGCACTAGAAAATGGTGTACTGCCAGGCTTCCCGCTAATCGGCATTAAAGAAAAACTTTTCGATGGTTCTTACAATGATGTTGACTCAAACGAAAAGCCAtt                                                                              >  1:1486199/1‑140 (MQ=255)

                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:1464700/1‑140 (MQ=255)

                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:1275826/1‑140 (MQ=255)

                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:1220363/1‑140 (MQ=255)

                                                                  aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGtttc                                                                     >  2:938542/1‑138 (MQ=255)

                                                                  aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgc                                                                     <  1:724358/140‑1 (MQ=255)

                                                                   tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  1:1967591/140‑1 (MQ=255)

                                                                   tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  2:1454072/140‑1 (MQ=255)

                                                                   tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGATTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  2:1526068/140‑1 (MQ=255)

                                                                     ccAGCTGTTCAAGCAGGTCTGGAAGATGCACTCGAAAATGGTGTACTAGCAGGCTTCCCGCTTATCGACATTAAAGCTAAACTTTTCGACGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTcgctgc                                                                  <  2:683296/140‑1 (MQ=255)

                                                                          tGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTa                                                             <  1:943878/140‑1 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCACAACTGTTCGATGGTTCTTACCATGATGTTGAGTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  2:2256237/1‑140 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  2:1244114/1‑140 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  1:2087221/1‑140 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  1:1758952/1‑140 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           <  2:1692916/140‑1 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  2:1276521/1‑140 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCGTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           <  2:347244/140‑1 (MQ=255)

                                                                              cAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGc                                                         >  1:2339442/1‑140 (MQ=255)

                                                                                 gCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCGTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAtt                                                      <  2:1423640/140‑1 (MQ=255)

                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  1:909051/1‑140 (MQ=255)

                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  2:1386615/1‑140 (MQ=255)

                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  1:407928/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGAt                                                                                                                    <  2:2137633/64‑1 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGAt                                                                                                                    >  1:2273760/1‑64 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGAt                                                                                                                    >  1:2137633/1‑64 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:1985805/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:2031964/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:2193080/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:2391100/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:1604688/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:315581/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:692138/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:746439/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:1337807/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:1041629/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:1087722/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:1095400/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:1128966/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:1008228/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:145987/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:1262508/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:817103/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:1185565/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTGCTATGGCATTGAAAAATGCAGTCa                                        >  1:1403410/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTAAAACGAAATGGCATTTAAAGTTGCTGCTTCTCTGGCATTGAAAAATGCAGTCa                                        >  2:50761/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGCTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:2251046/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGAAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:2233951/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACAt                                                                                                                                       >  2:2290352/1‑45 (MQ=255)

                                                                                                gCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTGCTATGGCATTGAAAAATGCAGTCAg                                       >  2:1013501/1‑140 (MQ=255)

                                                                                                 cACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                      <  2:1214629/140‑1 (MQ=255)

                                                                                                 cACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAAGGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                      <  2:363649/140‑1 (MQ=255)

                                                                                                    ttGAATATGGTGTACTGGCAGGCTTCCCGCTTAGGGGCATTATAGCAAAACTTTTAGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTTAAAAATGCAGTCAGCaaa                                   <  2:1481604/140‑1 (MQ=255)

                                                                                                     tGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                      >  2:2238347/1‑136 (MQ=255)

                                                                                                      taaaaTGGTGTACTGGCAGGCTTCCCGCTTATCGTCATTAAAGCAAAAATTTTCGATGGTTCTTACCATGATGTTGTCTCAAACGAAATGGCATTTATAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  2:1486199/139‑1 (MQ=255)

                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAACGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  1:1879010/140‑1 (MQ=255)

                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  2:2340418/140‑1 (MQ=255)

                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  1:2307729/140‑1 (MQ=255)

                                                                                                      gAAAATGGTGTACTAGCAGGCTTCCCGCTTATCGACATTAAAGCTAAACTTTTCGACGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  2:1782929/140‑1 (MQ=255)

                                                                                                      gAAAATGGTGTACTAGCAGGCTTCCCGATTATCGACATTAAAGCTAAACTTTTCGACGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  2:1537141/140‑1 (MQ=255)

                                                                                                           tGGTGTACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAAcc                            <  2:1726696/140‑1 (MQ=255)

                                                                                                                tACTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       >  2:653560/1‑140 (MQ=255)

                                                                                                                  cTGGCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAAACCAGttca                     >  2:1419603/1‑139 (MQ=255)

                                                                                                                     gCAGGCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTtcttct                  >  1:2325483/1‑140 (MQ=255)

                                                                                                                        ggCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  2:1900437/87‑1 (MQ=255)

                                                                                                                        ggCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa               >  2:1522905/1‑140 (MQ=255)

                                                                                                                        ggCTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAGTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa               >  2:498612/1‑140 (MQ=255)

                                                                                                                          cTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAc             >  2:73424/1‑140 (MQ=255)

                                                                                                                          cTTCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCCTCTTGAAc             >  1:852842/1‑140 (MQ=255)

                                                                                                                           ttCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATTGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAcc            <  1:2358316/140‑1 (MQ=255)

                                                                                                                           ttCCCGCTTATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAcc            <  1:2159117/140‑1 (MQ=255)

                                                                                                                                 ctgaTCGGCATTAAAGCAACACTTTTCGATGGTTCTTTCCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGAATTGAAAAATGCAGTCAGCAAATGTAACCAAGTTCTTCTTGAACCAATCAt      <  1:1603230/137‑1 (MQ=255)

                                                                                                                                 cgtATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt      <  2:2310643/138‑1 (MQ=255)

                                                                                                                                 cTTATCGGCATTAAAGCAAAACTTGTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt      <  1:1354404/140‑1 (MQ=255)

                                                                                                                                   tATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGa    <  2:1544094/140‑1 (MQ=255)

                                                                                                                                   tATCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGa    <  2:127557/140‑1 (MQ=255)

                                                                                                                                    aTCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGaa   >  2:908757/1‑140 (MQ=255)

                                                                                                                                    aTCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGaa   <  2:1464239/140‑1 (MQ=255)

                                                                                                                                    aTCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGaa   >  2:799675/1‑140 (MQ=255)

                                                                                                                                    aTCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGaa   <  1:595578/140‑1 (MQ=255)

                                                                                                                                    aTCGGCATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGaa   >  2:2050732/1‑140 (MQ=255)

AACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGAAA  >  NZ_CP034943/132050‑132322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`