Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,340,457 T→C 7.1% Q61Q (CAA→CAG epsG ← biofilm exopolysaccharide biosynthesis protein EpsG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,340,4570TC 7.1% 342.4 / 12.3 112Q61Q (CAA→CAGepsGbiofilm exopolysaccharide biosynthesis protein EpsG
Reads supporting (aligned to +/- strand):  ref base T (62/41);  new base C (4/4);  total (67/45)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

Only 100 of 113 total aligned reads displayed.

CGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCA  >  NZ_CP034943/3340319‑3340594
                                                                                                                                          |                                                                                                                                         
cGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGttt                                                                                                                                          >  1:224790/1‑140 (MQ=255)
   aTCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGaa                                                                                                                                       <  2:1034168/140‑1 (MQ=255)
   aTCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGaa                                                                                                                                       <  2:1255918/140‑1 (MQ=255)
     cTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAACCCCGGATCTGTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGaaaa                                                                                                                                     >  1:617719/1‑140 (MQ=255)
       tCCGTGAGGAACTTCATCAGCCAAAGGAGGGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAAGATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATc                                                                                                                                   <  2:845766/140‑1 (MQ=255)
            gatgaAATTCCTCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGACACCGAATTTCTGCGAGACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCGGTGc                                                                                                                              <  2:28517/140‑1 (MQ=255)
               gaAATTCATCAGCCAACGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           <  1:811310/140‑1 (MQ=255)
               gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACTTTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           <  1:2009564/140‑1 (MQ=255)
               gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           <  1:1972873/140‑1 (MQ=255)
               gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           <  1:2079519/140‑1 (MQ=255)
               gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           <  1:1573966/140‑1 (MQ=255)
               gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           <  1:1521612/140‑1 (MQ=255)
               gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           <  2:1161524/140‑1 (MQ=255)
               gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           <  1:1474934/140‑1 (MQ=255)
               gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           <  1:468170/140‑1 (MQ=255)
               gaAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           <  1:547902/140‑1 (MQ=255)
               gaAATTCATCAGCAAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           <  1:1341423/140‑1 (MQ=255)
                  aTTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGActc                                                                                                                        >  1:2127252/1‑140 (MQ=255)
                  aTTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGActc                                                                                                                        >  1:1169106/1‑140 (MQ=255)
                           ccAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGaa                                                                                                               >  1:1705858/1‑140 (MQ=255)
                                   gTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGac                                                                                                       <  2:1090257/140‑1 (MQ=255)
                                    tGCGGTAACGCCCGGGGCTGTCGCCGTTTTTGCTGTGCCGAAACCCAAAATCTGCCACACAGTTTGATAATCGCCCGCCAATTCGTACAGAAGTGTGTACGTTTGAAAATCCGTTCCGACTCTGTAACGAAGAACCGaca                                                                                                      >  1:2400411/1‑140 (MQ=255)
                                    tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                      >  2:1697745/1‑140 (MQ=255)
                                    tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                      >  1:790686/1‑140 (MQ=255)
                                    tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                      >  1:1367134/1‑140 (MQ=255)
                                    tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                      >  1:263145/1‑140 (MQ=255)
                                    tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                      >  2:1722298/1‑140 (MQ=255)
                                    tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                      >  1:2360903/1‑140 (MQ=255)
                                    tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                      >  1:1603153/1‑140 (MQ=255)
                                    tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                      >  1:1644065/1‑140 (MQ=255)
                                    tGCGGTAAAGCCCGGGTCTGTCGCCCTGTTTGCTGTGCCGCAACCGAATAGCTGCCACACAGTTTGATAAACGCCCGCCAATTCGTGCAGCGGTGTGTACGTGTGTAACTCCGTGCCGACTCTGTAACGAAGACCCGacc                                                                                                      >  1:2277626/1‑139 (MQ=255)
                                    tGCGGTAAACCCCGGATCTGTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGaca                                                                                                      >  2:309448/1‑140 (MQ=255)
                                    tGCGGTAAACCCCGGATCTGTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGaca                                                                                                      >  2:1770178/1‑140 (MQ=255)
                                     gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACCGTTTGATAATCGCCCGCCAATTCGTACCGCAGGGTGTGCGTTTGACAATCCGTGCCGCCTCTGGAACGAAGACCCGaccc                                                                                                     >  1:2123580/1‑138 (MQ=255)
                                     gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGccac                                                                                                     >  1:1312284/1‑140 (MQ=255)
                                     gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                                     >  1:23063/1‑140 (MQ=255)
                                     gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                                     <  2:236253/140‑1 (MQ=255)
                                     gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                                     >  1:1519390/1‑140 (MQ=255)
                                     gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGGGTACGGTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaccc                                                                                                     >  1:1469310/1‑138 (MQ=255)
                                        gTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAg                                                                                                  <  1:1722298/140‑1 (MQ=255)
                                         tAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGc                                                                                                 >  1:655160/1‑140 (MQ=255)
                                          aaaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGACTATCTGCCACACATTTTGATAATCGGCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGca                                                                                                <  1:728284/140‑1 (MQ=255)
                                          aaaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGca                                                                                                <  1:1749455/140‑1 (MQ=255)
                                           aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           >  2:2158781/1‑112 (MQ=255)
                                           aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           <  1:2158781/112‑1 (MQ=255)
                                           aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcac                                                                                               >  1:2411336/1‑140 (MQ=255)
                                           aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcac                                                                                               >  1:639124/1‑140 (MQ=255)
                                              cccGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAg                                                                                            <  2:1543671/140‑1 (MQ=255)
                                              cccGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGCAACGAAGACCCGACACGAGCACAAg                                                                                            >  1:324233/1‑140 (MQ=255)
                                                   gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                       >  1:2870/1‑140 (MQ=255)
                                                   gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                       >  1:1949896/1‑140 (MQ=255)
                                                   gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                       >  1:2971/1‑140 (MQ=255)
                                                   gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATAGCCCGCCCATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                       >  1:1950217/1‑140 (MQ=255)
                                                       gTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGaagcgag                                                                                   <  2:2391880/140‑1 (MQ=255)
                                                        tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGACGACCCGACACGAGCACAAGTGaagcaagc                                                                                  >  2:1988236/1‑140 (MQ=255)
                                                        tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                                  >  2:2265377/1‑140 (MQ=255)
                                                        tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                                  >  2:920246/1‑140 (MQ=255)
                                                        tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGACCACAAGTGaagcaagc                                                                                  >  1:2380248/1‑140 (MQ=255)
                                                         cGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCg                                                                                 <  1:240951/140‑1 (MQ=255)
                                                             gTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAAt                                                                             >  1:2185892/1‑140 (MQ=255)
                                                                     tgtgCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCa                                                                     <  1:1155452/140‑1 (MQ=255)
                                                                     tgtgCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGAAGCGAGCGGAATCCAGATCa                                                                     <  2:617719/140‑1 (MQ=255)
                                                                      ttgCCGAAACCGAAAATCTGCCACACATTTTGATAATCGCCCGCCACTTCGTACAGCACTGGGTACGTTTGCAAATCCGTGCCGACTCTGTAACGAAGACCCGACCCGAGCACCAGAGACGCCAGCGGAATCCCAATCac                                                                    >  2:1999423/2‑139 (MQ=255)
                                                                         ccGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           >  1:1969305/1‑82 (MQ=255)
                                                                         ccGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGa                                                                                                                           <  2:1969305/82‑1 (MQ=255)
                                                                              aCCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAt                                                            <  2:2430351/140‑1 (MQ=255)
                                                                               ccGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAtt                                                           <  2:1447306/140‑1 (MQ=255)
                                                                               ccGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAtt                                                           <  2:1644065/140‑1 (MQ=255)
                                                                                     atCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCg                                                     <  1:1494536/140‑1 (MQ=255)
                                                                                        tGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATa                                                  >  1:1089110/1‑140 (MQ=255)
                                                                                         gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                                 >  1:399751/1‑140 (MQ=255)
                                                                                         gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                                 >  2:2264173/1‑140 (MQ=255)
                                                                                         gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                                 >  2:2360961/1‑140 (MQ=255)
                                                                                                 ttttGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGc                                         <  1:1726619/140‑1 (MQ=255)
                                                                                                 ttttGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGATTGAAAATCCGGGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGc                                         <  2:554742/140‑1 (MQ=255)
                                                                                                      aTAATCGCCCGCCAATTCGTACATCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTg                                    <  2:2192178/140‑1 (MQ=255)
                                                                                                      aTAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTg                                    <  2:635241/140‑1 (MQ=255)
                                                                                                        aaTCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGaa                                  <  2:655160/140‑1 (MQ=255)
                                                                                                                 ccAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACgg                         <  2:790686/140‑1 (MQ=255)
                                                                                                                 ccAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACgg                         <  2:2360903/140‑1 (MQ=255)
                                                                                                                     ttCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGgccgc                     >  1:236234/1‑140 (MQ=255)
                                                                                                                       cGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGccgccg                   <  2:1071910/140‑1 (MQ=255)
                                                                                                                       cGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGccgccg                   <  2:2007136/140‑1 (MQ=255)
                                                                                                                              cagTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACAttt            >  2:1705941/1‑140 (MQ=255)
                                                                                                                                gtgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTg          >  2:1486958/1‑140 (MQ=255)
                                                                                                                                 tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg         >  2:426862/1‑140 (MQ=255)
                                                                                                                                 tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg         >  1:770226/1‑140 (MQ=255)
                                                                                                                                 tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg         >  1:1005835/1‑140 (MQ=255)
                                                                                                                                 tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg         >  1:2389168/1‑140 (MQ=255)
                                                                                                                                 tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg         >  1:930549/1‑140 (MQ=255)
                                                                                                                                 tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAGTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg         <  2:1705858/140‑1 (MQ=255)
                                                                                                                                   tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCACAAGCTTATTCGGCCGATACCCCGTGGCAAGTGCAGCGTCACGGCCGCCGCACATTTTGGcc       >  2:1807580/1‑139 (MQ=255)
                                                                                                                                   tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGCATCGTCACGGCCGCCGCACATTTTGGCa       >  1:739270/1‑140 (MQ=255)
                                                                                                                                   tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa       >  1:133061/1‑140 (MQ=255)
                                                                                                                                   tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa       >  1:2387116/1‑140 (MQ=255)
                                                                                                                                   tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa       >  1:475608/1‑140 (MQ=255)
                                                                                                                                   tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa       >  1:798432/1‑140 (MQ=255)
                                                                                                                                   tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa       >  1:791548/1‑140 (MQ=255)
                                                                                                                                      aCGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGAAGCGAGCGGAATCCAGATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGCGAATCATCACGGCCGCCGCACATTTTAGCGAAc    <  1:1770178/140‑1 (MQ=255)
                                                                                                                                      aCGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGAAGCGAGCGGAATCCAGATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGCGAATCATCACGGCCGCCGCACATTTTAGCGAAc    <  1:309448/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
CGGATCTTCCGTGATGAAATTCATCAGCCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCA  >  NZ_CP034943/3340319‑3340594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: