Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,340,478 G→A 5.6% Y54Y (TAC→TAT epsG ← biofilm exopolysaccharide biosynthesis protein EpsG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,340,4780GA 5.6% 413.4 / 9.1 128Y54Y (TAC→TATepsGbiofilm exopolysaccharide biosynthesis protein EpsG
Reads supporting (aligned to +/- strand):  ref base G (82/38);  new base A (3/4);  total (86/42)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 128 total aligned reads displayed.

CCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCC  >  NZ_CP034943/3340346‑3340615
                                                                                                                                    |                                                                                                                                         
ccAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGaa                                                                                                                                    <  1:674746/140‑1 (MQ=255)
ccAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGaa                                                                                                                                    >  1:1705858/1‑140 (MQ=255)
        gTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGac                                                                                                                            <  2:1090257/140‑1 (MQ=255)
         tGCGGTAACGCCCGGGGCTGTCGCCGTTTTTGCTGTGCCGAAACCCAAAATCTGCCACACAGTTTGATAATCGCCCGCCAATTCGTACAGAAGTGTGTACGTTTGAAAATCCGTTCCGACTCTGTAACGAAGAACCGaca                                                                                                                           >  1:2400411/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                           >  1:1367134/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                           >  2:1697745/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                           >  2:5362/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                           >  1:1447306/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                           >  1:790686/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                           >  1:1603153/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                           >  1:1644065/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                           >  1:263145/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCCTGTTTGCTGTGCCGCAACCGAATAGCTGCCACACAGTTTGATAAACGCCCGCCAATTCGTGCAGCGGTGTGTACGTGTGTAACTCCGTGCCGACTCTGTAACGAAGACCCGacc                                                                                                                           >  1:2277626/1‑139 (MQ=255)
         tGCGGTAAACCCCGGATCTGTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGaca                                                                                                                           >  2:309448/1‑140 (MQ=255)
         tGCGGTAAACCCCGGATCTGTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGaca                                                                                                                           >  2:1770178/1‑140 (MQ=255)
          gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACCGTTTGATAATCGCCCGCCAATTCGTACCGCAGGGTGTGCGTTTGACAATCCGTGCCGCCTCTGGAACGAAGACCCGaccc                                                                                                                          >  1:2123580/1‑138 (MQ=255)
          gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGccac                                                                                                                          >  1:1312284/1‑140 (MQ=255)
          gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                                                          <  2:236253/140‑1 (MQ=255)
          gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                                                          >  1:1519390/1‑140 (MQ=255)
          gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                                                          >  1:23063/1‑140 (MQ=255)
             gTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAg                                                                                                                       <  1:1722298/140‑1 (MQ=255)
              tAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGc                                                                                                                      >  1:655160/1‑140 (MQ=255)
               aaaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGACTATCTGCCACACATTTTGATAATCGGCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGca                                                                                                                     <  1:728284/140‑1 (MQ=255)
               aaaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGca                                                                                                                     <  1:1749455/140‑1 (MQ=255)
                aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcac                                                                                                                    >  1:2411336/1‑140 (MQ=255)
                aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcac                                                                                                                    >  1:639124/1‑140 (MQ=255)
                   cccGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCCGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcccacg                                                                                                                 >  2:9288/1‑138 (MQ=255)
                   cccGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAg                                                                                                                 <  2:1543671/140‑1 (MQ=255)
                        gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                                            >  1:2971/1‑140 (MQ=255)
                        gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                                            >  1:2870/1‑140 (MQ=255)
                        gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                                            >  1:1949896/1‑140 (MQ=255)
                        gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATAGCCCGCCCATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                                            >  1:1950217/1‑140 (MQ=255)
                            gTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGaagcgag                                                                                                        <  2:2391880/140‑1 (MQ=255)
                             tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGACGACCCGACACGAGCACAAGTGaagcaagc                                                                                                       >  2:1988236/1‑140 (MQ=255)
                             tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                                                       >  2:920246/1‑140 (MQ=255)
                             tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGACCACAAGTGaagcaagc                                                                                                       >  1:2380248/1‑140 (MQ=255)
                             tCGCCGTTTTTGCTGTGCCGAAACCGAATATATGCCACACATTTTGATAAACGCCCGCCAAATCGTACAGCAGTGTGTACGGTTGAAAATACGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                                                       >  1:969016/1‑140 (MQ=255)
                              cGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCg                                                                                                      <  1:240951/140‑1 (MQ=255)
                                          tgtgCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCa                                                                                          <  1:1155452/140‑1 (MQ=255)
                                           ttgCCGAAACCGAAAATCTGCCACACATTTTGATAATCGCCCGCCACTTCGTACAGCACTGGGTACGTTTGCAAATCCGTGCCGACTCTGTAACGAAGACCCGACCCGAGCACCAGAGACGCCAGCGGAATCCCAATCac                                                                                         >  2:1999423/2‑139 (MQ=255)
                                              ccGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACACGTGAAGCAAGCGGAATCCAAATCAAAAg                                                                                      >  1:2234779/1‑140 (MQ=255)
                                                          atCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCg                                                                          <  1:1660032/140‑1 (MQ=255)
                                                          atCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCg                                                                          <  1:1494536/140‑1 (MQ=255)
                                                             tGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATa                                                                       >  1:1089110/1‑140 (MQ=255)
                                                              gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                                                      >  1:399751/1‑140 (MQ=255)
                                                              gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                                                      >  2:2264173/1‑140 (MQ=255)
                                                                      ttttGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGc                                                              <  1:1726619/140‑1 (MQ=255)
                                                                      ttttGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGATTGAAAATCCGGGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGc                                                              <  2:554742/140‑1 (MQ=255)
                                                                           aTAATCGCCCGCCAATTCGTACATCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTg                                                         <  2:2192178/140‑1 (MQ=255)
                                                                           aTAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTg                                                         <  2:635241/140‑1 (MQ=255)
                                                                             aaTCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGaa                                                       <  2:655160/140‑1 (MQ=255)
                                                                                      ccAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACgg                                              <  2:2360903/140‑1 (MQ=255)
                                                                                      ccAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACgg                                              <  2:790686/140‑1 (MQ=255)
                                                                                          ttCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGgccgc                                          >  1:236234/1‑140 (MQ=255)
                                                                                            cGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGccgccg                                        <  2:1071910/140‑1 (MQ=255)
                                                                                                   cagTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACAttt                                 >  2:1705941/1‑140 (MQ=255)
                                                                                                     gtgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTg                               >  2:1486958/1‑140 (MQ=255)
                                                                                                      tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                              >  1:1005835/1‑140 (MQ=255)
                                                                                                      tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                              >  2:2271337/1‑140 (MQ=255)
                                                                                                      tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                              >  1:2389168/1‑140 (MQ=255)
                                                                                                      tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                              >  1:770226/1‑140 (MQ=255)
                                                                                                      tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAGTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                              <  2:1705858/140‑1 (MQ=255)
                                                                                                        ttaaCGTTTGAAAATCAGTGACGACTATCTAACGAAAACACCAAAAGAGCACAAGTGAAGCAACCTGAATCCAAATCAAAAGCTAAATCGGCCTATACCCACTGGCAAGAGAATAGTCACTGACGCAGCAAATTTTGGCa                            >  2:1580742/4‑140 (MQ=255)
                                                                                                        tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCACAAGCTTATTCGGCCGATACCCCGTGGCAAGTGCAGCGTCACGGCCGCCGCACATTTTGGcc                            >  2:1807580/1‑139 (MQ=255)
                                                                                                        tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTCTTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa                            >  1:373339/1‑140 (MQ=255)
                                                                                                        tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa                            >  1:2387116/1‑140 (MQ=255)
                                                                                                        tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa                            >  1:798432/1‑140 (MQ=255)
                                                                                                        tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa                            >  1:791548/1‑140 (MQ=255)
                                                                                                           aCGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGAAGCGAGCGGAATCCAGATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGCGAATCATCACGGCCGCCGCACATTTTAGCGAAc                         <  1:309448/140‑1 (MQ=255)
                                                                                                             gTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCa                       >  1:1972697/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAACTCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAggc                    >  2:1938015/1‑139 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGACTCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                    >  2:391384/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                    >  1:1791275/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                    >  2:2103663/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                    >  2:391809/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                    >  1:1415018/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                    >  2:639662/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTGTGGCACACCCGGa                    >  2:2102614/1‑140 (MQ=255)
                                                                                                                 gAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                   <  2:224790/140‑1 (MQ=255)
                                                                                                                 gAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                   <  2:1784932/140‑1 (MQ=255)
                                                                                                                 gAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                   <  2:1706944/140‑1 (MQ=255)
                                                                                                                 gAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                   <  2:2277626/140‑1 (MQ=255)
                                                                                                                 gAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                   <  1:1390338/140‑1 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCCCAAGTGAAGCAAGCGGACTCCAAATCACAAGCTGATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACAGTTTGGCCAACCAGGAcc                  >  2:2083951/1‑140 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                  >  1:609761/1‑140 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                  >  1:216616/1‑140 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                  >  1:216643/1‑140 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                  >  1:664045/1‑140 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                  >  1:498281/1‑140 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                   >  2:2329032/1‑139 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                   <  1:2329032/139‑1 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTCAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                  >  1:369104/1‑140 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGATTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                  >  2:648728/1‑140 (MQ=255)
                                                                                                                      tCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTTGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAat              <  2:2250354/140‑1 (MQ=255)
                                                                                                                          tGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAatatat          >  2:451049/1‑140 (MQ=255)
                                                                                                                              gACTCTGTAACGAAGACCCGACACGAGCAGAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACaa      <  2:266364/140‑1 (MQ=255)
                                                                                                                              gACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCAGATTTTGGCAAACCAGGAGCAAATATATACaa      <  2:1972697/140‑1 (MQ=255)
                                                                                                                              gACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACaa      <  2:133061/140‑1 (MQ=255)
                                                                                                                                ctctGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAAtt    <  2:2380248/140‑1 (MQ=255)
                                                                                                                                 tctGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTc   <  1:1697745/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                         
CCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCC  >  NZ_CP034943/3340346‑3340615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: