Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,340,484 A→G 5.4% L52L (CTT→CTC epsG ← biofilm exopolysaccharide biosynthesis protein EpsG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,340,4840AG 5.4% 416.7 / 9.4 129L52L (CTT→CTCepsGbiofilm exopolysaccharide biosynthesis protein EpsG
Reads supporting (aligned to +/- strand):  ref base A (82/40);  new base G (3/4);  total (85/44)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 133 total aligned reads displayed.

CCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTGA  >  NZ_CP034943/3340346‑3340623
                                                                                                                                          |                                                                                                                                           
ccAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGaa                                                                                                                                            >  1:1705858/1‑140 (MQ=255)
ccAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGaa                                                                                                                                            <  1:674746/140‑1 (MQ=255)
         tGCGGTAACGCCCGGGGCTGTCGCCGTTTTTGCTGTGCCGAAACCCAAAATCTGCCACACAGTTTGATAATCGCCCGCCAATTCGTACAGAAGTGTGTACGTTTGAAAATCCGTTCCGACTCTGTAACGAAGAACCGaca                                                                                                                                   >  1:2400411/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                   >  2:5362/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                   >  1:2360903/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                   >  1:263145/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                   >  1:1644065/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                   >  1:790686/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                   >  2:1697745/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                   >  2:1722298/1‑140 (MQ=255)
         tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                   >  1:1367134/1‑140 (MQ=255)
          gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACCGTTTGATAATCGCCCGCCAATTCGTACCGCAGGGTGTGCGTTTGACAATCCGTGCCGCCTCTGGAACGAAGACCCGaccc                                                                                                                                  >  1:2123580/1‑138 (MQ=255)
          gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGccac                                                                                                                                  >  1:1312284/1‑140 (MQ=255)
          gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                                                                  >  1:1519390/1‑140 (MQ=255)
          gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                                                                  <  2:236253/140‑1 (MQ=255)
          gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                                                                  >  1:23063/1‑140 (MQ=255)
          gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGGGTACGGTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaccc                                                                                                                                  >  1:1469310/1‑138 (MQ=255)
             gTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAg                                                                                                                               <  1:1722298/140‑1 (MQ=255)
              tAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGc                                                                                                                              >  1:655160/1‑140 (MQ=255)
               aaaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGACTATCTGCCACACATTTTGATAATCGGCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGca                                                                                                                             <  1:728284/140‑1 (MQ=255)
               aaaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGca                                                                                                                             <  1:1749455/140‑1 (MQ=255)
                aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcac                                                                                                                            >  1:639124/1‑140 (MQ=255)
                aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcac                                                                                                                            >  1:2411336/1‑140 (MQ=255)
                   cccGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCCGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcccacg                                                                                                                         >  2:9288/1‑138 (MQ=255)
                   cccGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGCAACGAAGACCCGACACGAGCACAAg                                                                                                                         >  1:324233/1‑140 (MQ=255)
                        gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                                                    >  1:2971/1‑140 (MQ=255)
                        gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                                                    >  1:2870/1‑140 (MQ=255)
                        gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATAGCCCGCCCATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                                                    >  1:1950217/1‑140 (MQ=255)
                            gTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGaagcgag                                                                                                                <  2:2391880/140‑1 (MQ=255)
                             tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGACGACCCGACACGAGCACAAGTGaagcaagc                                                                                                               >  2:1988236/1‑140 (MQ=255)
                             tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                                                               >  2:920246/1‑140 (MQ=255)
                             tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                                                               >  2:2265377/1‑140 (MQ=255)
                             tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGACCACAAGTGaagcaagc                                                                                                               >  1:2380248/1‑140 (MQ=255)
                             tCGCCGTTTTTGCTGTGCCGAAACCGAATATATGCCACACATTTTGATAAACGCCCGCCAAATCGTACAGCAGTGTGTACGGTTGAAAATACGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                                                               >  1:969016/1‑140 (MQ=255)
                              cGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCg                                                                                                              <  1:240951/140‑1 (MQ=255)
                                  gTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAAt                                                                                                          >  1:2185892/1‑140 (MQ=255)
                                          tgtgCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCa                                                                                                  <  1:1155452/140‑1 (MQ=255)
                                          tgtgCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGAAGCGAGCGGAATCCAGATCa                                                                                                  <  2:617719/140‑1 (MQ=255)
                                           ttgCCGAAACCGAAAATCTGCCACACATTTTGATAATCGCCCGCCACTTCGTACAGCACTGGGTACGTTTGCAAATCCGTGCCGACTCTGTAACGAAGACCCGACCCGAGCACCAGAGACGCCAGCGGAATCCCAATCac                                                                                                 >  2:1999423/2‑139 (MQ=255)
                                              ccGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACACGTGAAGCAAGCGGAATCCAAATCAAAAg                                                                                              >  1:2234779/1‑140 (MQ=255)
                                                   aCCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAt                                                                                         <  2:2430351/140‑1 (MQ=255)
                                                    ccGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAtt                                                                                        <  2:1447306/140‑1 (MQ=255)
                                                    ccGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAtt                                                                                        <  2:1644065/140‑1 (MQ=255)
                                                          atCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCg                                                                                  <  1:1494536/140‑1 (MQ=255)
                                                          atCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCg                                                                                  <  1:1660032/140‑1 (MQ=255)
                                                             tGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATa                                                                               >  1:1089110/1‑140 (MQ=255)
                                                              gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                                                              >  2:2360961/1‑140 (MQ=255)
                                                                      ttttGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGATTGAAAATCCGGGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGc                                                                      <  2:554742/140‑1 (MQ=255)
                                                                           aTAATCGCCCGCCAATTCGTACATCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTg                                                                 <  2:2192178/140‑1 (MQ=255)
                                                                             aaTCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGaa                                                               <  2:655160/140‑1 (MQ=255)
                                                                                      ccAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACgg                                                      <  2:2360903/140‑1 (MQ=255)
                                                                                      ccAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACgg                                                      <  2:790686/140‑1 (MQ=255)
                                                                                            cGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGccgccg                                                <  2:1071910/140‑1 (MQ=255)
                                                                                                   cagTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACAttt                                         >  2:1705941/1‑140 (MQ=255)
                                                                                                     gtgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTg                                       >  2:1486958/1‑140 (MQ=255)
                                                                                                      tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                      >  2:2271337/1‑140 (MQ=255)
                                                                                                      tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                      >  1:1005835/1‑140 (MQ=255)
                                                                                                      tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                      >  2:1873058/1‑140 (MQ=255)
                                                                                                      tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                      >  2:426862/1‑140 (MQ=255)
                                                                                                      tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                      >  1:930549/1‑140 (MQ=255)
                                                                                                      tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                      >  1:770226/1‑140 (MQ=255)
                                                                                                      tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                      >  1:2389168/1‑140 (MQ=255)
                                                                                                        ttaaCGTTTGAAAATCAGTGACGACTATCTAACGAAAACACCAAAAGAGCACAAGTGAAGCAACCTGAATCCAAATCAAAAGCTAAATCGGCCTATACCCACTGGCAAGAGAATAGTCACTGACGCAGCAAATTTTGGCa                                    >  2:1580742/4‑140 (MQ=255)
                                                                                                        tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCACAAGCTTATTCGGCCGATACCCCGTGGCAAGTGCAGCGTCACGGCCGCCGCACATTTTGGcc                                    >  2:1807580/1‑139 (MQ=255)
                                                                                                        tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTCTTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa                                    >  1:373339/1‑140 (MQ=255)
                                                                                                        tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa                                    >  1:791548/1‑140 (MQ=255)
                                                                                                        tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa                                    >  1:2387116/1‑140 (MQ=255)
                                                                                                        tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa                                    >  1:475608/1‑140 (MQ=255)
                                                                                                           aCGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGAAGCGAGCGGAATCCAGATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGCGAATCATCACGGCCGCCGCACATTTTAGCGAAc                                 <  1:1770178/140‑1 (MQ=255)
                                                                                                             gTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCa                               >  1:1972697/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGTCTCTGTAACGAAGAGCCGACACGAGCACAAGTGAAGCAAGCCGAATCCAAATCAAAAGCTTATTCGGCCGATACCCAGTGGCAAGTGAATCTTGACGGCCGCCGCACATTTTGGCACACCAGGa                            >  1:2065140/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGACTCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                            >  2:391384/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                            >  1:1415018/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                            >  2:2103663/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                            >  2:391809/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                            >  1:1791275/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                            >  2:639662/1‑140 (MQ=255)
                                                                                                                tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTGTGGCACACCCGGa                            >  2:2102614/1‑140 (MQ=255)
                                                                                                                 gAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                           <  2:2277626/140‑1 (MQ=255)
                                                                                                                 gAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                           <  2:1706944/140‑1 (MQ=255)
                                                                                                                 gAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                           <  1:1390338/140‑1 (MQ=255)
                                                                                                                 gAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                           <  2:1784932/140‑1 (MQ=255)
                                                                                                                 gAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                           <  2:224790/140‑1 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCCCAAGTGAAGCAAGCGGACTCCAAATCACAAGCTGATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACAGTTTGGCCAACCAGGAcc                          >  2:2083951/1‑140 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                          >  1:498281/1‑140 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                          >  1:216643/1‑140 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                          >  1:664045/1‑140 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                          >  1:1634223/1‑140 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                           <  1:2329032/139‑1 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTCAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                          >  1:369104/1‑140 (MQ=255)
                                                                                                                  aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGATTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                          >  2:648728/1‑140 (MQ=255)
                                                                                                                      tCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTTGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAat                      <  2:2250354/140‑1 (MQ=255)
                                                                                                                              gACTCTGTAACGAAGACCCGACACGAGCAGAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACaa              <  2:266364/140‑1 (MQ=255)
                                                                                                                              gACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCAGATTTTGGCAAACCAGGAGCAAATATATACaa              <  2:1972697/140‑1 (MQ=255)
                                                                                                                              gACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACaa              <  2:133061/140‑1 (MQ=255)
                                                                                                                                 tctGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTc           >  1:1712608/1‑140 (MQ=255)
                                                                                                                                         gAAGACCCGACCCGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTg   <  1:639662/140‑1 (MQ=255)
                                                                                                                                         gAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTg   <  1:2103663/140‑1 (MQ=255)
                                                                                                                                          aaGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTGa  <  1:524011/140‑1 (MQ=255)
                                                                                                                                          aaGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTGa  <  1:2150736/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CCAAAGGAGTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTGA  >  NZ_CP034943/3340346‑3340623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: