Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,340,490 C→T 5.1% S50S (TCG→TCA epsG ← biofilm exopolysaccharide biosynthesis protein EpsG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,340,4900CT 5.1% 458.3 / 8.7 138S50S (TCG→TCAepsGbiofilm exopolysaccharide biosynthesis protein EpsG
Reads supporting (aligned to +/- strand):  ref base C (85/46);  new base T (3/4);  total (88/50)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 140 total aligned reads displayed.

GTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTGACGGCA  >  NZ_CP034943/3340354‑3340628
                                                                                                                                        |                                                                                                                                          
gTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGac                                                                                                                                         <  2:1090257/140‑1 (MQ=255)
 tGCGGTAACGCCCGGGGCTGTCGCCGTTTTTGCTGTGCCGAAACCCAAAATCTGCCACACAGTTTGATAATCGCCCGCCAATTCGTACAGAAGTGTGTACGTTTGAAAATCCGTTCCGACTCTGTAACGAAGAACCGaca                                                                                                                                        >  1:2400411/1‑140 (MQ=255)
 tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                        >  1:1644065/1‑140 (MQ=255)
 tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                        >  2:1722298/1‑140 (MQ=255)
 tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                        >  2:1697745/1‑140 (MQ=255)
 tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                        >  1:790686/1‑140 (MQ=255)
 tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                        >  1:2360903/1‑140 (MQ=255)
 tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                        >  1:1603153/1‑140 (MQ=255)
 tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                        >  1:1447306/1‑140 (MQ=255)
 tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                        >  2:5362/1‑140 (MQ=255)
 tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                        >  1:1367134/1‑140 (MQ=255)
 tGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaca                                                                                                                                        >  1:263145/1‑140 (MQ=255)
 tGCGGTAAAGCCCGGGTCTGTCGCCCTGTTTGCTGTGCCGCAACCGAATAGCTGCCACACAGTTTGATAAACGCCCGCCAATTCGTGCAGCGGTGTGTACGTGTGTAACTCCGTGCCGACTCTGTAACGAAGACCCGacc                                                                                                                                        >  1:2277626/1‑139 (MQ=255)
  gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACCGTTTGATAATCGCCCGCCAATTCGTACCGCAGGGTGTGCGTTTGACAATCCGTGCCGCCTCTGGAACGAAGACCCGaccc                                                                                                                                       >  1:2123580/1‑138 (MQ=255)
  gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                                                                       >  1:1519390/1‑140 (MQ=255)
  gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGacac                                                                                                                                       <  2:236253/140‑1 (MQ=255)
  gCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGGGTACGGTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGaccc                                                                                                                                       >  1:1469310/1‑138 (MQ=255)
     gTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAg                                                                                                                                    <  1:1722298/140‑1 (MQ=255)
        aaGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcac                                                                                                                                 >  1:639124/1‑140 (MQ=255)
           cccGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCCGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGcccacg                                                                                                                              >  2:9288/1‑138 (MQ=255)
           cccGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAg                                                                                                                              <  2:1543671/140‑1 (MQ=255)
                gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                                                         >  1:1949896/1‑140 (MQ=255)
                gTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaag                                                                                                                         >  1:2870/1‑140 (MQ=255)
                    gTCGCTGTTTTCGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAACAGCGTGTACGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGaagcgag                                                                                                                     <  2:2391880/140‑1 (MQ=255)
                     tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGACGACCCGACACGAGCACAAGTGaagcaagc                                                                                                                    >  2:1988236/1‑140 (MQ=255)
                     tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                                                                    >  2:2265377/1‑140 (MQ=255)
                     tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGaagcaagc                                                                                                                    >  2:920246/1‑140 (MQ=255)
                     tCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGACCACAAGTGaagcaagc                                                                                                                    >  1:2380248/1‑140 (MQ=255)
                      cGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCg                                                                                                                   <  1:240951/140‑1 (MQ=255)
                          gTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAAt                                                                                                               >  1:2185892/1‑140 (MQ=255)
                                  tgtgCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCa                                                                                                       <  1:1155452/140‑1 (MQ=255)
                                      ccGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACACGTGAAGCAAGCGGAATCCAAATCAAAAg                                                                                                   >  1:2234779/1‑140 (MQ=255)
                                           aCCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAt                                                                                              <  2:2430351/140‑1 (MQ=255)
                                            ccGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAtt                                                                                             <  2:1644065/140‑1 (MQ=255)
                                                  atCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCg                                                                                       <  1:1660032/140‑1 (MQ=255)
                                                     tGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATa                                                                                    >  1:1089110/1‑140 (MQ=255)
                                                      gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                                                                   >  1:399751/1‑140 (MQ=255)
                                                      gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                                                                   >  2:2360961/1‑140 (MQ=255)
                                                      gCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATAc                                                                                   >  2:2264173/1‑140 (MQ=255)
                                                              ttttGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGc                                                                           <  1:1726619/140‑1 (MQ=255)
                                                              ttttGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGATTGAAAATCCGGGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGc                                                                           <  2:554742/140‑1 (MQ=255)
                                                                   aTAATCGCCCGCCAATTCGTACATCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTg                                                                      <  2:2192178/140‑1 (MQ=255)
                                                                   aTAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTg                                                                      <  2:635241/140‑1 (MQ=255)
                                                                     aaTCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGaa                                                                    <  2:655160/140‑1 (MQ=255)
                                                                              ccAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACgg                                                           <  2:790686/140‑1 (MQ=255)
                                                                              ccAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACgg                                                           <  2:2360903/140‑1 (MQ=255)
                                                                                    cGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGccgccg                                                     <  2:1071910/140‑1 (MQ=255)
                                                                                    cGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGccgccg                                                     <  2:2007136/140‑1 (MQ=255)
                                                                                             gtgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTg                                            >  2:1486958/1‑140 (MQ=255)
                                                                                              tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                           >  1:930549/1‑140 (MQ=255)
                                                                                              tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                           >  1:2389168/1‑140 (MQ=255)
                                                                                              tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                           >  2:1873058/1‑140 (MQ=255)
                                                                                              tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                           >  2:2271337/1‑140 (MQ=255)
                                                                                              tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                           >  1:1005835/1‑140 (MQ=255)
                                                                                              tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                           >  1:770226/1‑140 (MQ=255)
                                                                                              tgtgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTAGTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTgg                                           <  2:1705858/140‑1 (MQ=255)
                                                                                                tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCACAAGCTTATTCGGCCGATACCCCGTGGCAAGTGCAGCGTCACGGCCGCCGCACATTTTGGcc                                         >  2:1807580/1‑139 (MQ=255)
                                                                                                tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTCTTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa                                         >  1:373339/1‑140 (MQ=255)
                                                                                                tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGCATCGTCACGGCCGCCGCACATTTTGGCa                                         >  1:739270/1‑140 (MQ=255)
                                                                                                tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa                                         >  1:798432/1‑140 (MQ=255)
                                                                                                tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa                                         >  1:2387116/1‑140 (MQ=255)
                                                                                                tgtACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCa                                         >  1:133061/1‑140 (MQ=255)
                                                                                                   aCGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGAAGCGAGCGGAATCCAGATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGCGAATCATCACGGCCGCCGCACATTTTAGCGAAc                                      <  1:1770178/140‑1 (MQ=255)
                                                                                                   aCGTCTGAAAATCCGTGCCGACTCGATAGCGGAGACCTGACACGAGCACAAGTGAAGCGAGCGGAATCCAGATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGCGAATCATCACGGCCGCCGCACATTTTAGCGAAc                                      <  1:309448/140‑1 (MQ=255)
                                                                                                     gTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCa                                    >  1:1972697/1‑140 (MQ=255)
                                                                                                        tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAATCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                                 >  1:2428767/1‑140 (MQ=255)
                                                                                                        tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGACTCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                                 >  2:391384/1‑140 (MQ=255)
                                                                                                        tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                                 >  1:1415018/1‑140 (MQ=255)
                                                                                                        tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                                 >  2:639662/1‑140 (MQ=255)
                                                                                                        tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                                 >  2:391809/1‑140 (MQ=255)
                                                                                                        tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGa                                 >  1:1791275/1‑140 (MQ=255)
                                                                                                        tGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTGTGGCACACCCGGa                                 >  2:2102614/1‑140 (MQ=255)
                                                                                                         gAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                                <  2:1706944/140‑1 (MQ=255)
                                                                                                         gAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                                <  2:2277626/140‑1 (MQ=255)
                                                                                                         gAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                                <  2:1784932/140‑1 (MQ=255)
                                                                                                          aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCCCAAGTGAAGCAAGCGGACTCCAAATCACAAGCTGATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACAGTTTGGCCAACCAGGAcc                               >  2:2083951/1‑140 (MQ=255)
                                                                                                          aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                               >  1:663944/1‑140 (MQ=255)
                                                                                                          aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                               >  1:216616/1‑140 (MQ=255)
                                                                                                          aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                               >  2:712005/1‑140 (MQ=255)
                                                                                                          aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                               >  1:664045/1‑140 (MQ=255)
                                                                                                          aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                               >  1:609761/1‑140 (MQ=255)
                                                                                                          aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                               >  1:498281/1‑140 (MQ=255)
                                                                                                          aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAc                                <  1:2329032/139‑1 (MQ=255)
                                                                                                          aaaaTCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTCAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGAcc                               >  1:369104/1‑140 (MQ=255)
                                                                                                              tCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTTGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAat                           <  2:2250354/140‑1 (MQ=255)
                                                                                                                  tGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAatatat                       >  2:451049/1‑140 (MQ=255)
                                                                                                                      gACTCTGTAACGAAGACCCGACACGAGCAGAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACaa                   <  2:266364/140‑1 (MQ=255)
                                                                                                                      gACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCAGATTTTGGCAAACCAGGAGCAAATATATACaa                   <  2:1972697/140‑1 (MQ=255)
                                                                                                                      gACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACaa                   <  2:133061/140‑1 (MQ=255)
                                                                                                                        ctctGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAAtt                 <  2:2380248/140‑1 (MQ=255)
                                                                                                                         tctGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTc                <  1:1697745/140‑1 (MQ=255)
                                                                                                                          ctGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTcc               <  2:2185892/140‑1 (MQ=255)
                                                                                                                                 gAAGACCCGACCCGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTg        <  1:639662/140‑1 (MQ=255)
                                                                                                                                 gAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTg        <  1:2102614/140‑1 (MQ=255)
                                                                                                                                 gAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTg        <  1:2103663/140‑1 (MQ=255)
                                                                                                                                  aaGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTGa       <  1:2150736/140‑1 (MQ=255)
                                                                                                                                    gACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTGACg     <  1:1985887/140‑1 (MQ=255)
                                                                                                                                    gACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTGACg     <  1:492905/140‑1 (MQ=255)
                                                                                                                                     aCCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTGACgg    <  1:309167/140‑1 (MQ=255)
                                                                                                                                       ccGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTGACGGCa  >  2:261837/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
GTGCGGTAAAGCCCGGGTCTGTCGCCGTTTTTGCTGTGCCGAAACCGAATATCTGCCACACATTTTGATAATCGCCCGCCAATTCGTACAGCAGTGTGTACGTTTGAAAATCCGTGCCGACTCTGTAACGAAGACCCGACACGAGCACAAGTGAAGCAAGCGGAATCCAAATCAAAAGCTTATTCGGCCGATACCCCGTGGCAAGTGAATCGTCACGGCCGCCGCACATTTTGGCAAACCAGGACCAAATATATACAATTCCCATATTGACGGCA  >  NZ_CP034943/3340354‑3340628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: