Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,523,104 A→G 5.0% L140L (TTG→CTG)  EO946_RS18120 ← CotH kinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,523,1040AG 5.0% 383.5 / 7.0 119L140L (TTG→CTG) EO946_RS18120CotH kinase family protein
Reads supporting (aligned to +/- strand):  ref base A (63/50);  new base G (3/3);  total (66/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

Only 100 of 119 total aligned reads displayed.

ATAAAAATCGTCTTCCTCAGTCCCAGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAAT  >  NZ_CP034943/3522967‑3523237
                                                                                                                                         |                                                                                                                                      
ataaaAATCGTCTTCCTCAGTCCCAGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAAct                                                                                                                                      >  1:1601474/1‑140 (MQ=255)
ataaaAATCGTCTTCCTCAGTCCCAGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAAACGCCCCGTCCGCCAAct                                                                                                                                      >  1:52224/1‑140 (MQ=255)
           cgtCCTCAGTCCCAGTCTTCTTTTCATCTCCACGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTGTTTCGCt                                                                                                                          <  1:2349083/138‑1 (MQ=255)
           cTTCCTCAGTCCCAGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCt                                                                                                                          <  1:837519/140‑1 (MQ=255)
           cTTCCTCAGTCCCAGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCt                                                                                                                          <  1:357849/140‑1 (MQ=255)
                 cAGTCCCAGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCtaaataa                                                                                                                    <  2:87344/140‑1 (MQ=255)
                    tCCCAGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATACAAt                                                                                                                 >  1:1701965/1‑140 (MQ=255)
                    tCCCAGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAAt                                                                                                                 >  1:1701860/1‑140 (MQ=255)
                    tCCCAGTCTTCTTTTCATATACAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAAt                                                                                                                 >  1:1342353/1‑140 (MQ=255)
                        aGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAACTTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCag                                                                                                             >  2:334315/1‑139 (MQ=255)
                        aGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCAt                                                                                                             >  1:1417332/1‑140 (MQ=255)
                         gTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATc                                                                                                            >  1:2349158/1‑140 (MQ=255)
                              cttTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACgg                                                                                                       >  1:69351/1‑140 (MQ=255)
                              cttTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACgg                                                                                                       >  2:1601746/1‑140 (MQ=255)
                              cttTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACgg                                                                                                       >  2:1381907/1‑140 (MQ=255)
                              cttTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACgg                                                                                                       >  2:1052435/1‑140 (MQ=255)
                                  tCATATCCAAGCTCACGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttc                                                                                                   <  1:1548934/140‑1 (MQ=255)
                                  tCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCTGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttc                                                                                                   <  1:320499/140‑1 (MQ=255)
                                  tCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttc                                                                                                   <  1:1542835/140‑1 (MQ=255)
                                    atatCCAAGCTCACGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAtgcaa                                                                                                 >  1:2061324/1‑140 (MQ=255)
                                      atCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagt                                                                                               >  2:1171700/1‑140 (MQ=255)
                                      atCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagt                                                                                               >  1:884644/1‑140 (MQ=255)
                                      atCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagt                                                                                               >  1:1956934/1‑140 (MQ=255)
                                       tCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagtt                                                                                              <  1:1132739/140‑1 (MQ=255)
                                       tCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagtt                                                                                              <  1:923951/140‑1 (MQ=255)
                                       tCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagtt                                                                                              <  2:6553/140‑1 (MQ=255)
                                       tCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagtt                                                                                              <  2:453403/140‑1 (MQ=255)
                                       tCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagtt                                                                                              <  2:334939/140‑1 (MQ=255)
                                           aGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagttcaag                                                                                          >  1:69323/1‑140 (MQ=255)
                                           aGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagttcaag                                                                                          >  1:897972/1‑140 (MQ=255)
                                           aGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagttcaag                                                                                          >  1:1594134/1‑140 (MQ=255)
                                                aaGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                     <  2:2208216/140‑1 (MQ=255)
                                                aaGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                     <  2:1345494/140‑1 (MQ=255)
                                                aaGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                     <  1:186439/140‑1 (MQ=255)
                                                aaGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTATTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                     <  1:1116979/140‑1 (MQ=255)
                                                aaGCGACGTATTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCACCCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                     <  1:1981455/140‑1 (MQ=255)
                                                 aGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAAc                                                                                    >  2:1754515/1‑140 (MQ=255)
                                                 aGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAAc                                                                                    >  2:466121/1‑140 (MQ=255)
                                                 aGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAAc                                                                                    >  2:1838639/1‑140 (MQ=255)
                                                 aGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAAc                                                                                    >  2:232573/1‑140 (MQ=255)
                                                      cGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCtt                                                                               >  1:1885348/1‑140 (MQ=255)
                                                       gTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGACATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTc                                                                              >  1:1358020/1‑140 (MQ=255)
                                                        tCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                     <  2:1153019/133‑1 (MQ=255)
                                                        tCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                     >  1:1153019/1‑133 (MQ=255)
                                                        tCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                     <  2:543600/132‑1 (MQ=255)
                                                            cGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCAttct                                                                         >  2:1700095/1‑140 (MQ=255)
                                                            cGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCAttct                                                                         <  2:1012006/140‑1 (MQ=255)
                                                            cGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCAttct                                                                         <  2:1012001/140‑1 (MQ=255)
                                                                   ctgtCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCAtt                                                                  <  1:1726113/137‑1 (MQ=255)
                                                                   cTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCAtt                                                                  <  1:720688/140‑1 (MQ=255)
                                                                   cTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCAtt                                                                  <  1:328264/140‑1 (MQ=255)
                                                                           aTCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTa                                                          >  1:137247/1‑140 (MQ=255)
                                                                           aTCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTa                                                          >  1:1313970/1‑140 (MQ=255)
                                                                           aTCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTa                                                          <  1:1171700/140‑1 (MQ=255)
                                                                           aTCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTa                                                          >  1:5280/1‑140 (MQ=255)
                                                                             cGCTCATCAGATATAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGACAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTaaa                                                        <  2:52224/140‑1 (MQ=255)
                                                                               cTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTaaaaa                                                      >  1:1526643/1‑140 (MQ=255)
                                                                                  atcaGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAacac                                                   >  1:543941/1‑140 (MQ=255)
                                                                                       agagaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAAct                                              >  1:2084740/1‑140 (MQ=255)
                                                                                        gagaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAActc                                             <  1:959596/140‑1 (MQ=255)
                                                                                        gagaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAActc                                             <  1:658591/140‑1 (MQ=255)
                                                                                        gagaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAActc                                             <  2:1358020/140‑1 (MQ=255)
                                                                                        gagaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAActc                                             <  1:2234142/140‑1 (MQ=255)
                                                                                          gaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTg                                           <  2:1342353/140‑1 (MQ=255)
                                                                                          gaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTg                                           >  1:598714/1‑140 (MQ=255)
                                                                                          gaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTg                                           >  1:1439252/1‑140 (MQ=255)
                                                                                          gaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTg                                           >  1:1044494/1‑140 (MQ=255)
                                                                                                     catcatcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGAc                                >  2:1079629/1‑140 (MQ=255)
                                                                                                     catcatcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGAc                                >  2:1637854/1‑140 (MQ=255)
                                                                                                      atcatcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACa                               >  1:2206852/1‑140 (MQ=255)
                                                                                                      atcatcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACa                               >  1:1955059/1‑140 (MQ=255)
                                                                                                       tcatcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTACGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTATTCCCATTCACCGTTAAAAACACAAACTCTGCCTTTGGAGACac                              >  2:1246973/1‑139 (MQ=255)
                                                                                                        cctcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTGTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCAGTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGc                             <  2:1576026/138‑1 (MQ=255)
                                                                                                        catcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGc                             <  2:897972/140‑1 (MQ=255)
                                                                                                        catcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCAGTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGc                             <  2:1594134/140‑1 (MQ=255)
                                                                                                            aaCCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTcc                         >  1:1207868/1‑140 (MQ=255)
                                                                                                               cgcgTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCaa                      >  2:925954/1‑140 (MQ=255)
                                                                                                                 cgTAAAAAACCGCGCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTGCCCATTCAGCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGc                    >  1:2096683/1‑140 (MQ=255)
                                                                                                                 cgTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCCCCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGc                    >  2:2353889/1‑140 (MQ=255)
                                                                                                                 cgTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAATAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGc                    >  1:1117669/1‑140 (MQ=255)
                                                                                                                 cgTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGc                    >  2:2088500/1‑140 (MQ=255)
                                                                                                                 cgTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGc                    >  1:2091888/1‑140 (MQ=255)
                                                                                                                 cgTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGc                    >  1:9477/1‑140 (MQ=255)
                                                                                                                 catAAAAAATCGCGCCATCAGCCAGCTTCCTTTTCGCCAAATAATATTCATCTACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCATCTTTACAAACGCAAACTCTGCCTTTGgaga                                 <  2:2112617/125‑1 (MQ=255)
                                                                                                                 catAAAAAATCGCGCCATCAGCCAGCTTCCTTTTCGCCAAATAATATTCATCTACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCATCTTTACAAACGCAAACTCTGCCTTTGgaga                                 >  1:886204/3‑127 (MQ=255)
                                                                                                                 catAAAAAATCGCGCCATCAGCCAGCTTCCTTTTCGCCAAATAATATTCATCTACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCATCTTTACAAACGCAAACTCTGCCTTTGgaga                                 <  2:886204/125‑1 (MQ=255)
                                                                                                                 catAAAAAATCGCGCCATCAGCCAGCTTCCTTTTCGCCAAATAATATTCATCTACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCATCTTTACAAACGCAAACTCTGCCTTTGgaga                                 <  2:886165/125‑1 (MQ=255)
                                                                                                                 catAAAAAATCGCGCCATCAGCCAGCTTCCTTTTCGCCAAATAATATTCATCTACGGATTCAAGTACAAGATAAACCCCTTCATTCTTCCCATTCATCTTTACAAACGCAAACTCTGCCTTTGgaga                                 >  1:2112617/3‑127 (MQ=255)
                                                                                                                   tAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAACACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGctc                  <  1:635221/140‑1 (MQ=255)
                                                                                                                   tAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGctc                  <  1:1926693/140‑1 (MQ=255)
                                                                                                                   tAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGctc                  <  1:327100/140‑1 (MQ=255)
                                                                                                                   tAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCGAAATAAAATTCATCGACGGATTCAAGTTCAAGATACACCCCTTCATTCTTCCCATTCAGCTTTAAGAACACAAACTCTGCCGTTGGCGACAGCGTCCCCAGctc                  >  1:2315093/1‑140 (MQ=255)
                                                                                                                   tAAAAAACCGCCCCGTCCGCCAACTTCCAGTTCGCTAAATCATATTCATCGACGGATTCAAGTTCAATATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGctc                  <  1:662885/140‑1 (MQ=255)
                                                                                                                           cGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGaaaa          >  2:2390148/1‑140 (MQ=255)
                                                                                                                              cccGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAAt       >  2:348758/1‑140 (MQ=255)
                                                                                                                              cccGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAAt       >  2:1561171/1‑140 (MQ=255)
                                                                                                                              cccGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAAt       >  2:2160271/1‑140 (MQ=255)
                                                                                                                              cccGTCCGCCAACTTCTTTTTCGCTAAATAAAATTAATCGACGGATTCAAGTTCAAGATAAACCACTTCAGTCTGCCCATTCACCTTTAAAAACAAAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAAt       >  2:1561153/1‑140 (MQ=255)
                                                                                                                                cGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATcc     <  2:1526643/140‑1 (MQ=255)
                                                                                                                                   ccGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAAt  <  2:2341691/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
ATAAAAATCGTCTTCCTCAGTCCCAGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAAT  >  NZ_CP034943/3522967‑3523237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: