Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,523,124 A→T 5.4% F133Y (TTT→TAT)  EO946_RS18120 ← CotH kinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,523,1240AT 5.4% 408.7 / 9.9 124F133Y (TTT→TAT) EO946_RS18120CotH kinase family protein
Reads supporting (aligned to +/- strand):  ref base A (64/52);  new base T (3/4);  total (67/57)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

TCCCAGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTTCTCATCATGGAAG  >  NZ_CP034943/3522987‑3523262
                                                                                                                                          |                                                                                                                                          
tCCCAGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTCAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAAt                                                                                                                                          >  2:1520952/1‑140 (MQ=255)
tCCCAGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAAt                                                                                                                                          >  1:1701860/1‑140 (MQ=255)
    aGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAACTTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCag                                                                                                                                      >  2:334315/1‑139 (MQ=255)
    aGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCAt                                                                                                                                      >  1:1417332/1‑140 (MQ=255)
          cttTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACgg                                                                                                                                >  2:1052435/1‑140 (MQ=255)
          cttTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACgg                                                                                                                                >  1:69351/1‑140 (MQ=255)
          cttTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACgg                                                                                                                                >  2:1601746/1‑140 (MQ=255)
              tCATATCCAAGCTCACGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttc                                                                                                                            <  1:1548934/140‑1 (MQ=255)
              tCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCTGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttc                                                                                                                            <  1:320499/140‑1 (MQ=255)
                atatCCAAGCTCACGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAtgcaa                                                                                                                          >  1:2061324/1‑140 (MQ=255)
                  atCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagt                                                                                                                        >  2:1171700/1‑140 (MQ=255)
                  atCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagt                                                                                                                        >  1:1956934/1‑140 (MQ=255)
                  atCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagt                                                                                                                        >  1:884644/1‑140 (MQ=255)
                   tCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagtt                                                                                                                       <  2:334939/140‑1 (MQ=255)
                   tCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagtt                                                                                                                       <  2:453205/140‑1 (MQ=255)
                   tCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagtt                                                                                                                       <  2:453403/140‑1 (MQ=255)
                   tCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagtt                                                                                                                       <  1:923951/140‑1 (MQ=255)
                   tCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagtt                                                                                                                       <  1:18737/140‑1 (MQ=255)
                       aGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGAttcaagttcaag                                                                                                                   >  1:1594134/1‑140 (MQ=255)
                            aaGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                                              <  2:2208216/140‑1 (MQ=255)
                            aaGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                                              <  2:1345494/140‑1 (MQ=255)
                            aaGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                                              <  1:186439/140‑1 (MQ=255)
                            aaGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTATTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                                              <  1:1116979/140‑1 (MQ=255)
                             aGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAAc                                                                                                             >  2:1754515/1‑140 (MQ=255)
                             aGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAAc                                                                                                             >  2:1838639/1‑140 (MQ=255)
                             aGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAAc                                                                                                             >  2:466121/1‑140 (MQ=255)
                                   gTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGACATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTc                                                                                                       >  1:1358020/1‑140 (MQ=255)
                                    tCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                                              >  1:1153019/1‑133 (MQ=255)
                                    tCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                                              >  1:543600/1‑132 (MQ=255)
                                    tCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATaaa                                                                                                              <  2:543600/132‑1 (MQ=255)
                                        cGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCAttct                                                                                                  <  2:1012001/140‑1 (MQ=255)
                                        cGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCAttct                                                                                                  <  2:1012006/140‑1 (MQ=255)
                                               ctgtCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCAtt                                                                                           <  1:1726113/137‑1 (MQ=255)
                                               cTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCAtt                                                                                           <  1:720688/140‑1 (MQ=255)
                                                       ctCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGAGAAACCCCGTCATTCGTCCCATTCACCTTTa                                                                                   >  1:1555609/2‑140 (MQ=255)
                                                       aTCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTa                                                                                   >  1:137247/1‑140 (MQ=255)
                                                       aTCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTa                                                                                   >  1:137254/1‑140 (MQ=255)
                                                       aTCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTa                                                                                   <  1:1171700/140‑1 (MQ=255)
                                                       aTCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTa                                                                                   >  1:1313970/1‑140 (MQ=255)
                                                         cGCTCATCAGATATAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGACAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTaaa                                                                                 <  2:52224/140‑1 (MQ=255)
                                                         cGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCCCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTaaa                                                                                 <  2:1601474/140‑1 (MQ=255)
                                                           cTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTaaaaa                                                                               >  1:1526643/1‑140 (MQ=255)
                                                              atcaGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAacac                                                                            >  1:543941/1‑140 (MQ=255)
                                                                   agagaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAAct                                                                       >  1:2084740/1‑140 (MQ=255)
                                                                    gagaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAActc                                                                      <  1:658591/140‑1 (MQ=255)
                                                                    gagaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAActc                                                                      <  2:1358020/140‑1 (MQ=255)
                                                                    gagaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAActc                                                                      <  1:2234142/140‑1 (MQ=255)
                                                                      gaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTg                                                                    >  1:1044494/1‑140 (MQ=255)
                                                                      gaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTg                                                                    >  1:1439252/1‑140 (MQ=255)
                                                                      gaAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTg                                                                    >  1:598714/1‑140 (MQ=255)
                                                                                 catcatcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGAc                                                         >  2:1079629/1‑140 (MQ=255)
                                                                                 catcatcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGAc                                                         >  2:1637854/1‑140 (MQ=255)
                                                                                  atcatcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACa                                                        >  1:1955059/1‑140 (MQ=255)
                                                                                   tcatcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTACGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTATTCCCATTCACCGTTAAAAACACAAACTCTGCCTTTGGAGACac                                                       >  2:1246973/1‑139 (MQ=255)
                                                                                    cctcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTGTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCAGTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGc                                                      <  2:1576026/138‑1 (MQ=255)
                                                                                    catcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGc                                                      <  2:897972/140‑1 (MQ=255)
                                                                                    catcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGc                                                      <  1:2373479/140‑1 (MQ=255)
                                                                                    catcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGc                                                      <  2:120482/140‑1 (MQ=255)
                                                                                    catcaACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCAGTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGc                                                      <  2:69323/140‑1 (MQ=255)
                                                                                    catcaACCGCCTAAAAAACCGCCCCGTCCGCCAACTTCTTTGTCGCTAAATAACATTCAGCGACGGAGTCAAGTTCAAGATAAACCCCTGCATTCTTCCCAGTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGc                                                      <  1:982083/140‑1 (MQ=255)
                                                                                        aaCCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTcc                                                  >  1:1207868/1‑140 (MQ=255)
                                                                                           cgcgTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCaa                                               >  2:925954/1‑140 (MQ=255)
                                                                                            gcgTAAAAAACCGCCCCGTCCGCAAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATGCGTCCCATGCACCTTTCGAAACACAAACTCGGCCTTTGGAGACAGCGTCCCAAg                                              >  1:2146540/1‑140 (MQ=255)
                                                                                             cgTAAAAAACCGCGCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTGCCCATTCAGCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGc                                             >  1:2096683/1‑140 (MQ=255)
                                                                                             cgTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCCCCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGc                                             >  2:2353889/1‑140 (MQ=255)
                                                                                             cgTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAATAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGc                                             >  1:1117669/1‑140 (MQ=255)
                                                                                             cgTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGc                                             >  1:9477/1‑140 (MQ=255)
                                                                                             cgTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGc                                             >  2:2088500/1‑140 (MQ=255)
                                                                                             cgTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGc                                             >  1:2029106/1‑140 (MQ=255)
                                                                                             cgTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGGTCAAGATAAACCCCTTCATTCTTCCCATTCACCGTTAAAAACACACACTCTGCCTTTGGCGACCGCGTCCCAAGc                                             >  2:2352797/1‑140 (MQ=255)
                                                                                             catAAAAAATCGCGCCATCAGCCAGCTTCCTTTTCGCCAAATAATATTCATCTACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCATCTTTACAAACGCAAACTCTGCCTTTGgaga                                                          >  1:886165/3‑127 (MQ=255)
                                                                                             catAAAAAATCGCGCCATCAGCCAGCTTCCTTTTCGCCAAATAATATTCATCTACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCATCTTTACAAACGCAAACTCTGCCTTTGgaga                                                          <  2:886165/125‑1 (MQ=255)
                                                                                             catAAAAAATCGCGCCATCAGCCAGCTTCCTTTTCGCCAAATAATATTCATCTACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCATCTTTACAAACGCAAACTCTGCCTTTGgaga                                                          >  1:886204/3‑127 (MQ=255)
                                                                                             catAAAAAATCGCGCCATCAGCCAGCTTCCTTTTCGCCAAATAATATTCATCTACGGATTCAAGTACAAGATAAACCCCTTCATTCTTCCCATTCATCTTTACAAACGCAAACTCTGCCTTTGgaga                                                          >  1:2112617/3‑127 (MQ=255)
                                                                                               tAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAACACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGctc                                           <  1:635221/140‑1 (MQ=255)
                                                                                               tAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGctc                                           <  1:1926693/140‑1 (MQ=255)
                                                                                               tAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGctc                                           <  1:327100/140‑1 (MQ=255)
                                                                                               tAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCGAAATAAAATTCATCGACGGATTCAAGTTCAAGATACACCCCTTCATTCTTCCCATTCAGCTTTAAGAACACAAACTCTGCCGTTGGCGACAGCGTCCCCAGctc                                           >  1:2315093/1‑140 (MQ=255)
                                                                                               tAAAAAACCGCCCCGTCCGCCAACTTCCAGTTCGCTAAATCATATTCATCGACGGATTCAAGTTCAATATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGctc                                           <  1:662885/140‑1 (MQ=255)
                                                                                                          cccGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAAt                                >  2:348758/1‑140 (MQ=255)
                                                                                                          cccGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAAt                                >  2:2160271/1‑140 (MQ=255)
                                                                                                          cccGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAAt                                >  2:205151/1‑140 (MQ=255)
                                                                                                          cccGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAAt                                >  2:1561171/1‑140 (MQ=255)
                                                                                                            cGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATcc                              <  2:1526643/140‑1 (MQ=255)
                                                                                                               ccGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAAt                           <  2:2341691/140‑1 (MQ=255)
                                                                                                                              ttCGCTAAATAAAATTCATCGACGTATTCAAGTTCAAGATAAACCCATTCATTCTTCCCATTCACCTTTAAAAACACAAACTATTCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTtct             <  1:2279848/140‑1 (MQ=255)
                                                                                                                              ttCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTtct             <  1:441150/140‑1 (MQ=255)
                                                                                                                              ttCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTtct             <  1:805793/140‑1 (MQ=255)
                                                                                                                              ttCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTtct             <  2:69351/140‑1 (MQ=255)
                                                                                                                              ttCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCGTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTtct             <  2:1361620/140‑1 (MQ=255)
                                                                                                                              ttCGATATATCAAATTCCTCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTACCATTCACCTTTAAAAACACACACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTtct             <  2:2146540/140‑1 (MQ=255)
                                                                                                                                  cTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGc                                                      >  1:489041/1‑95 (MQ=255)
                                                                                                                                  cTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAAGTTGTTTCtcatc         >  2:1911895/1‑140 (MQ=255)
                                                                                                                                       taaaATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTTCTCATCATGGa    <  1:1700095/140‑1 (MQ=255)
                                                                                                                                         aaaTTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTTCTCATCATGGAAg  <  1:122481/140‑1 (MQ=255)
                                                                                                                                         aaaTTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTTCTCATCATGGAAg  <  2:1439252/140‑1 (MQ=255)
                                                                                                                                         aaaTTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTTCTCATCATGGAAg  <  2:1564177/140‑1 (MQ=255)
                                                                                                                                         aaaTTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTTCTCATCATGGAAg  <  1:1160712/140‑1 (MQ=255)
                                                                                                                                         aaaTTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTTCTCATCATGGAAg  <  1:925954/140‑1 (MQ=255)
                                                                                                                                         aaaTTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCGTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTTCTCATCATGGAAg  <  2:598714/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TCCCAGTCTTCTTTTCATATCCAAGCTCAAGCGACGTCTTCGTTTCCCTTTCCAAATCGCTCATCAGAGAGAAATTAGCATCATCATCAACCGCGTAAAAAACCGCCCCGTCCGCCAACTTCTTTTTCGCTAAATAAAATTCATCGACGGATTCAAGTTCAAGATAAACCCCTTCATTCTTCCCATTCACCTTTAAAAACACAAACTCTGCCTTTGGAGACAGCGTCCCAAGCTCTGAGAAAAAATCCAATGACAATTTGTTTCTCATCATGGAAG  >  NZ_CP034943/3522987‑3523262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: