BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,564,437	T→A	5.0%	S265S (TCT→TCA)	EO946_RS21110 →	histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,564,437	0	T	A	5.0%	339.7 / 7.1	99	S265S (TCT→TCA)	EO946_RS21110	histidine kinase
Reads supporting (aligned to +/- strand): ref base T (48/46); new base A (3/2); total (51/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 101 total aligned reads displayed.

TCTTCACGGCGTCAGCAAGAAAAGAGGCGGAGGATGGATCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGG  >  NZ_CP034943/3564299‑3564576

tcttcACGGCGTCAGCAAGAAAAGAGGCGGAGGATGGATCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTg                                                                                                                                            >  2:1370991/1‑140 (MQ=255)

     aCGGCGTCAGCAAGAAAAGAGGCGGAGGATGGATCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGAt                                                                                                                                       <  2:1470047/140‑1 (MQ=255)

     aCGGCGTCAGCAAGAAAAGAGGCGGAGGATGGATCAAGCTGACAGCGAAAAAACCAAGCGAGCATGGGTATTACCTCAAAGTAGAAGATAACGGGGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGAt                                                                                                                                       <  2:1502417/140‑1 (MQ=255)

         cGTCAGCAAGAAAAGAGGCGGAGGATGGATCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCg                                                                                                                                   >  1:99704/1‑140 (MQ=255)

         cGTCAGCAAGAAAAGAGGCGGAGGATGGATCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGACGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCg                                                                                                                                   >  1:1574868/1‑140 (MQ=255)

           tCAGCAAGAAAAGAGGCGGAGGATGGATCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGAt                                                                                                                                 >  2:1178972/1‑140 (MQ=255)

                             gaggaTGGATCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGaaa                                                                                                               >  1:978556/1‑140 (MQ=255)

                                  tggatCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAatat                                                                                                          >  1:1508819/1‑140 (MQ=255)

                                     atCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCaa                                                                                                       >  1:2414714/1‑140 (MQ=255)

                                              aCAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCt                                                                                              >  1:2418725/1‑140 (MQ=255)

                                              aCAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCt                                                                                              >  1:1567811/1‑140 (MQ=255)

                                              aCAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCt                                                                                              >  1:1951687/1‑140 (MQ=255)

                                              aCAGCTAAAAAACAAAGCAAAAATGAGTATCACATCAAAGTAGAGGATAACGGTCCCGGGATAACTCCGGAAAAACAAATAGATCTCTTATCAACGGATTTTGATCGCAGCGTCGGATTGAAAAATATCAATCAGCGGCt                                                                                              >  1:199337/1‑140 (MQ=255)

                                              aCAGCTAAAAAACAAAGCAAAAATGAGTATCACATCAAAGTAGAGGATAACGGTCCCGGGATAACTCCGGAAAAACAAATAGATCTCTTATCAACGGATTTTGATCGCAGCGTCGGATTGAAAAATATCAATCAGCGGCt                                                                                              >  1:1548464/1‑140 (MQ=255)

                                                            aaGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCg                                                                                >  1:2283576/1‑140 (MQ=255)

                                                                  gaATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCg                                                                          <  1:507694/140‑1 (MQ=255)

                                                                  gaATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCg                                                                          <  1:1528911/140‑1 (MQ=255)

                                                                  gaATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCg                                                                          <  1:1325029/140‑1 (MQ=255)

                                                                     tGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAAt                                                                       <  2:533885/140‑1 (MQ=255)

                                                                       ggTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTg                                                                     <  2:1864336/140‑1 (MQ=255)

                                                                       ggTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTg                                                                     <  2:567119/140‑1 (MQ=255)

                                                                       ggTATTACATCAAAGTACAAGATAAGGGGGCCGCCATAGCTCGGGAAAAACGAATCGATCTCTTATCTGCGGATTTCGTTCGCAGCGTCGTATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTg                                                                     <  2:1471623/140‑1 (MQ=255)

                                                                            tACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGAt                                                                >  1:1546880/1‑140 (MQ=255)

                                                                               aTCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcag                                                             <  1:2320721/140‑1 (MQ=255)

                                                                                tCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagc                                                            >  2:1579497/1‑140 (MQ=255)

                                                                                tCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagc                                                            >  2:1369108/1‑140 (MQ=255)

                                                                                tCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGAGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAAGTGAAGATcagc                                                            >  2:1957041/1‑140 (MQ=255)

                                                                                 cAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagca                                                           >  1:1634776/1‑140 (MQ=255)

                                                                                 cAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagca                                                           >  1:1242811/1‑140 (MQ=255)

                                                                                  acaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                          <  1:283205/138‑1 (MQ=255)

                                                                                  aaaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                          <  1:944889/140‑1 (MQ=255)

                                                                                  aaaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                          <  1:999929/140‑1 (MQ=255)

                                                                                  aaaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                          <  1:1011570/140‑1 (MQ=255)

                                                                                  aaaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                          <  1:2166841/140‑1 (MQ=255)

                                                                                  aaaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                          <  2:1840035/140‑1 (MQ=255)

                                                                                  aaaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                          <  1:1607784/140‑1 (MQ=255)

                                                                                  aaaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTATTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                          <  1:997028/140‑1 (MQ=255)

                                                                                  aaaGTAGAAGATAACGCTGCCGGCATAGCTCCGGAAAAACGACAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                          <  2:1911770/140‑1 (MQ=255)

                                                                                   aaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCagcagc                                                         >  1:1715788/1‑140 (MQ=255)

                                                                                   aaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCagcagc                                                         >  1:241644/1‑140 (MQ=255)

                                                                                   aaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCagcagc                                                         >  2:44855/1‑140 (MQ=255)

                                                                                         aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                   >  2:1876740/1‑140 (MQ=255)

                                                                                         aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                   >  2:763324/1‑140 (MQ=255)

                                                                                         aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                   <  1:755173/140‑1 (MQ=255)

                                                                                         aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                   >  2:979502/1‑140 (MQ=255)

                                                                                         aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                   >  1:550943/1‑140 (MQ=255)

                                                                                         aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                   >  1:1441042/1‑140 (MQ=255)

                                                                                         aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                   >  1:1705666/1‑140 (MQ=255)

                                                                                         aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                   >  1:1738544/1‑140 (MQ=255)

                                                                                         aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                   >  1:2051027/1‑140 (MQ=255)

                                                                                              aaCGGTGCCGTAATAGATCCGGAAAAACGAAAAGATATCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATAAACAAGCGGATGAAACATTTTTGCTGAAGAGAATTGAAGATCATCAGCACTCCGGATGc                                              >  2:799824/1‑140 (MQ=255)

                                                                                              aaCGGTGCCGGCATAGCTCCGGACAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTGTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGc                                              >  2:2232751/1‑140 (MQ=255)

                                                                                              aaCGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagca                                                           <  2:34596/127‑1 (MQ=255)

                                                                                              aaCGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagca                                                           >  1:34596/1‑127 (MQ=255)

                                                                                              aaCGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGc                                              >  2:1484619/1‑140 (MQ=255)

                                                                                                cGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGa                                                                                                                     >  1:1109201/1‑67 (MQ=255)

                                                                                                cGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGa                                                                                                                     <  2:1109201/67‑1 (MQ=255)

                                                                                                cGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCg                                            >  2:1469565/1‑140 (MQ=255)

                                                                                                 ggTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCgg                                           <  2:363847/140‑1 (MQ=255)

                                                                                                   tGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCa                                         >  1:346214/1‑140 (MQ=255)

                                                                                                    gCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCAc                                        <  2:2366052/140‑1 (MQ=255)

                                                                                                     ccGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCAcc                                       >  2:275812/1‑140 (MQ=255)

                                                                                                     ccGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCAcc                                       >  1:1830502/1‑140 (MQ=255)

                                                                                                     ccGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCAcc                                       >  1:606206/1‑140 (MQ=255)

                                                                                                     ccGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCAcc                                       >  2:137222/1‑140 (MQ=255)

                                                                                                     ccGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCAcc                                       >  1:67534/1‑140 (MQ=255)

                                                                                                     ccGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCAcc                                       >  2:534610/1‑140 (MQ=255)

                                                                                                       ggCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACctc                                     <  2:1034493/140‑1 (MQ=255)

                                                                                                       ggCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACctc                                     <  1:2312649/140‑1 (MQ=255)

                                                                                                       ggCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACctc                                     <  2:1285622/140‑1 (MQ=255)

                                                                                                       ggCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACctc                                     <  2:1574868/140‑1 (MQ=255)

                                                                                                       ggCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACctc                                     <  2:618013/140‑1 (MQ=255)

                                                                                                           tAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCa                                                                                                    >  1:1507587/1‑73 (MQ=255)

                                                                                                           tAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCa                                                                                                    <  2:1507587/73‑1 (MQ=255)

                                                                                                            aacTCCGGAAAAACAAATAGATCTCTTATCAACGGATTTTGATCGCAGCGTCGGATTGAAAAATATCAATCAGCGGCTGAAACATTTTTGCGGAAGCGAGTTGATGATCAGCAGCACTCCGGATGCAGGCACCTCTGtct                                <  2:199337/138‑3 (MQ=255)

                                                                                                            aacTCCGGAAAAACAAATAGATCTCTTATCAACGGATTTTGATCGCAGCGTCGGATTGAAAAATATCAATCAGCGGCTGAAACATTTTTGCGGAAGCGAGTTGATGATCAGCAGCACTCCGGATGCAGGCACCTCTGtct                                <  2:1548464/138‑3 (MQ=255)

                                                                                                             gCTCCGGACAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATAATTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTc                               >  1:2311015/1‑140 (MQ=255)

                                                                                                             gCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTc                               >  1:2157974/1‑140 (MQ=255)

                                                                                                             gCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTc                               >  1:2165544/1‑140 (MQ=255)

                                                                                                             gCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTc                               >  1:2342668/1‑140 (MQ=255)

                                                                                                             gCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTc                               >  2:1974877/1‑140 (MQ=255)

                                                                                                              cTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCa                              <  2:346214/140‑1 (MQ=255)

                                                                                                                 cGGAAAAACGAAAAGATCTCTTATCTGCTGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATg                           <  1:266243/140‑1 (MQ=255)

                                                                                                                 cGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATg                           <  2:1567811/140‑1 (MQ=255)

                                                                                                                 cGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATg                           <  2:1951687/140‑1 (MQ=255)

                                                                                                                 cGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATg                           <  2:2418725/140‑1 (MQ=255)

                                                                                                                 cGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATg                           >  1:49433/1‑140 (MQ=255)

                                                                                                                 aggAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATg                           <  1:2172088/139‑1 (MQ=255)

                                                                                                                     aaaaCGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCtca                       <  1:1507340/140‑1 (MQ=255)

                                                                                                                     aaaaCGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCtca                       <  2:1441042/140‑1 (MQ=255)

                                                                                                                     aaaaCGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCtca                       <  1:1391327/140‑1 (MQ=255)

                                                                                                                         caaataGATCTCTTATCAACGGATTTTGATCGCAGCGTCGGATTGAAAAATATCAATCAGCGGCTGAAACATTTTTGCGGAAGCGAGTTGATGATCAGCAGCACTCCGGATGCAGGCACCTCTGTCTCAATGCtcattca                   >  2:2289872/6‑140 (MQ=255)

                                                                                                                          gAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCtcattcat                  <  2:978556/140‑1 (MQ=255)

                                                                                                                               gATCTCTTATCTGCGTATTTCGATCGCAGCGTGGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGc             <  1:144517/140‑1 (MQ=255)

                                                                                                                               gATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGTATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGc             <  1:1503947/140‑1 (MQ=255)

                                                                                                                               gATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGc             <  1:1265648/140‑1 (MQ=255)

                                                                                                                               gATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGc             <  1:522586/140‑1 (MQ=255)

                                                                                                                                    ctTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGaaa        <  1:2166962/140‑1 (MQ=255)

                                                                                                                                          tGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAACTAACgg  >  2:1992125/1‑140 (MQ=255)

                                                                                                                                          tGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACgg  >  2:620598/1‑140 (MQ=255)

TCTTCACGGCGTCAGCAAGAAAAGAGGCGGAGGATGGATCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGG  >  NZ_CP034943/3564299‑3564576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`