Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,564,446 C→T 5.5% F268F (TTC→TTT EO946_RS21110 → histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,564,4460CT 5.5% 358.8 / 6.9 109F268F (TTC→TTTEO946_RS21110histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (53/50);  new base T (3/3);  total (56/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 119 total aligned reads displayed.

CGTCAGCAAGAAAAGAGGCGGAGGATGGATCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGAAAGCCCCA  >  NZ_CP034943/3564308‑3564585
                                                                                                                                          |                                                                                                                                           
cGTCAGCAAGAAAAGAGGCGGAGGATGGATCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGACGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCg                                                                                                                                            >  1:1574868/1‑140 (MQ=255)
  tCAGCAAGAAAAGAGGCGGAGGATGGATCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGAt                                                                                                                                          >  2:1178972/1‑140 (MQ=255)
                    gaggaTGGATCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGaaa                                                                                                                        >  1:978556/1‑140 (MQ=255)
                            atCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCaa                                                                                                                >  1:2414714/1‑140 (MQ=255)
                                     aCAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCt                                                                                                       >  1:2418725/1‑140 (MQ=255)
                                     aCAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCt                                                                                                       >  1:1951687/1‑140 (MQ=255)
                                     aCAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCt                                                                                                       >  1:1567811/1‑140 (MQ=255)
                                     aCAGCTAAAAAACAAAGCAAAAATGAGTATCACATCAAAGTAGAGGATAACGGTCCCGGGATAACTCCGGAAAAACAAATAGATCTCTTATCAACGGATTTTGATCGCAGCGTCGGATTGAAAAATATCAATCAGCGGCt                                                                                                       >  1:1548464/1‑140 (MQ=255)
                                                   aaGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCg                                                                                         >  1:2283576/1‑140 (MQ=255)
                                                         gaATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCg                                                                                   <  1:507694/140‑1 (MQ=255)
                                                         gaATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCg                                                                                   <  1:1528911/140‑1 (MQ=255)
                                                         gaATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCg                                                                                   <  1:1325029/140‑1 (MQ=255)
                                                            tGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAAt                                                                                <  2:533885/140‑1 (MQ=255)
                                                              ggTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTg                                                                              <  2:1864336/140‑1 (MQ=255)
                                                              ggTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTg                                                                              <  2:567119/140‑1 (MQ=255)
                                                              ggTATTACATCAAAGTACAAGATAAGGGGGCCGCCATAGCTCGGGAAAAACGAATCGATCTCTTATCTGCGGATTTCGTTCGCAGCGTCGTATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTg                                                                              <  2:1471623/140‑1 (MQ=255)
                                                                   tACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGAt                                                                         >  1:1546880/1‑140 (MQ=255)
                                                                      aTCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcag                                                                      <  1:2320721/140‑1 (MQ=255)
                                                                       tCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagc                                                                     >  2:1369108/1‑140 (MQ=255)
                                                                       tCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagc                                                                     >  2:1579497/1‑140 (MQ=255)
                                                                       tCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGAGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAAGTGAAGATcagc                                                                     >  2:1957041/1‑140 (MQ=255)
                                                                        cAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagca                                                                    >  1:1242811/1‑140 (MQ=255)
                                                                        cAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagca                                                                    >  1:1634776/1‑140 (MQ=255)
                                                                         acaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                                   <  1:283205/138‑1 (MQ=255)
                                                                         aaaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                                   <  1:999929/140‑1 (MQ=255)
                                                                         aaaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                                   <  1:2166841/140‑1 (MQ=255)
                                                                         aaaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                                   <  1:944889/140‑1 (MQ=255)
                                                                         aaaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                                   <  2:1840035/140‑1 (MQ=255)
                                                                         aaaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                                   <  1:1011570/140‑1 (MQ=255)
                                                                         aaaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTATTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagcag                                                                   <  1:997028/140‑1 (MQ=255)
                                                                          aaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCagcagc                                                                  >  1:1715788/1‑140 (MQ=255)
                                                                          aaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCagcagc                                                                  >  2:44855/1‑140 (MQ=255)
                                                                          aaGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCagcagc                                                                  >  1:241644/1‑140 (MQ=255)
                                                                                aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                            <  1:755173/140‑1 (MQ=255)
                                                                                aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                            >  1:550943/1‑140 (MQ=255)
                                                                                aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                            >  2:1876740/1‑140 (MQ=255)
                                                                                aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                            >  2:763324/1‑140 (MQ=255)
                                                                                aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                            >  2:979502/1‑140 (MQ=255)
                                                                                aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                            >  1:1441042/1‑140 (MQ=255)
                                                                                aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                            >  1:1705666/1‑140 (MQ=255)
                                                                                aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                            >  1:1738544/1‑140 (MQ=255)
                                                                                aagaTAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCg                                                            >  1:2051027/1‑140 (MQ=255)
                                                                                     aaCGGTGCCGTAATAGATCCGGAAAAACGAAAAGATATCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATAAACAAGCGGATGAAACATTTTTGCTGAAGAGAATTGAAGATCATCAGCACTCCGGATGc                                                       >  2:799824/1‑140 (MQ=255)
                                                                                     aaCGGTGCCGGCATAGCTCCGGACAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTGTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGc                                                       >  2:2232751/1‑140 (MQ=255)
                                                                                     aaCGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagca                                                                    >  1:34596/1‑127 (MQ=255)
                                                                                     aaCGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATcagca                                                                    <  2:34596/127‑1 (MQ=255)
                                                                                       cGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGa                                                                                                                              >  1:1109201/1‑67 (MQ=255)
                                                                                       cGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGa                                                                                                                              <  2:1109201/67‑1 (MQ=255)
                                                                                       cGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCg                                                     >  2:1469565/1‑140 (MQ=255)
                                                                                        ggTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCgg                                                    <  2:363847/140‑1 (MQ=255)
                                                                                          tGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCa                                                  >  1:346214/1‑140 (MQ=255)
                                                                                           gCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCAc                                                 <  2:2366052/140‑1 (MQ=255)
                                                                                            ccGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCAcc                                                >  1:67534/1‑140 (MQ=255)
                                                                                            ccGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCAcc                                                >  1:1830502/1‑140 (MQ=255)
                                                                                            ccGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCAcc                                                >  2:137222/1‑140 (MQ=255)
                                                                                              ggCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACctc                                              <  2:1285622/140‑1 (MQ=255)
                                                                                              ggCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACctc                                              <  2:1034493/140‑1 (MQ=255)
                                                                                              ggCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACctc                                              <  2:1574868/140‑1 (MQ=255)
                                                                                              ggCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACctc                                              <  2:618013/140‑1 (MQ=255)
                                                                                                  tAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCa                                                                                                             <  2:1507587/73‑1 (MQ=255)
                                                                                                  tAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCa                                                                                                             >  1:1507587/1‑73 (MQ=255)
                                                                                                   aacTCCGGAAAAACAAATAGATCTCTTATCAACGGATTTTGATCGCAGCGTCGGATTGAAAAATATCAATCAGCGGCTGAAACATTTTTGCGGAAGCGAGTTGATGATCAGCAGCACTCCGGATGCAGGCACCTCTGtct                                         <  2:1548464/138‑3 (MQ=255)
                                                                                                    gCTCCGGACAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATAATTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTc                                        >  1:2311015/1‑140 (MQ=255)
                                                                                                    gCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTc                                        >  1:2157974/1‑140 (MQ=255)
                                                                                                    gCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTc                                        >  1:2165544/1‑140 (MQ=255)
                                                                                                    gCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTc                                        >  2:1974877/1‑140 (MQ=255)
                                                                                                    gCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTc                                        >  1:2342668/1‑140 (MQ=255)
                                                                                                     cTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCa                                       <  2:346214/140‑1 (MQ=255)
                                                                                                        cGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATg                                    >  1:49433/1‑140 (MQ=255)
                                                                                                        cGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATg                                    <  2:2418725/140‑1 (MQ=255)
                                                                                                        cGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATg                                    <  2:1951687/140‑1 (MQ=255)
                                                                                                        cGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATg                                    <  2:1567811/140‑1 (MQ=255)
                                                                                                            aaaaCGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCtca                                <  1:1507340/140‑1 (MQ=255)
                                                                                                            aaaaCGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCtca                                <  2:1441042/140‑1 (MQ=255)
                                                                                                                caaataGATCTCTTATCAACGGATTTTGATCGCAGCGTCGGATTGAAAAATATCAATCAGCGGCTGAAACATTTTTGCGGAAGCGAGTTGATGATCAGCAGCACTCCGGATGCAGGCACCTCTGTCTCAATGCtcattca                            >  2:2289872/6‑140 (MQ=255)
                                                                                                                 gAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCtcattcat                           <  2:978556/140‑1 (MQ=255)
                                                                                                                      gATCTCTTATCTGCGTATTTCGATCGCAGCGTGGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGc                      <  1:144517/140‑1 (MQ=255)
                                                                                                                      gATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGTATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGc                      <  1:1503947/140‑1 (MQ=255)
                                                                                                                      gATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGc                      <  1:522586/140‑1 (MQ=255)
                                                                                                                      gATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGc                      <  1:1265648/140‑1 (MQ=255)
                                                                                                                           ctTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGaaa                 <  1:2166962/140‑1 (MQ=255)
                                                                                                                                 tGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAACTAACgg           >  2:1992125/1‑140 (MQ=255)
                                                                                                                                 tGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACgg           >  2:620598/1‑140 (MQ=255)
                                                                                                                                  gCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGa          <  1:137222/140‑1 (MQ=255)
                                                                                                                                  gCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGa          <  1:275812/140‑1 (MQ=255)
                                                                                                                                  gCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGa          <  2:2157974/140‑1 (MQ=255)
                                                                                                                                  gCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGa          <  1:534610/140‑1 (MQ=255)
                                                                                                                                   cGGATTTTGATCGCAGCGTCGGATTGAAAAATATCAATCAGCGGCTGAAACATTTTTGCGGAAGCGAGTTGATGATCAGCAGCACTCCGGATGCAGGCACCTCTGTCTCAATGCTCATTCATCTCGCTGAaacaacagga         <  1:2289872/140‑5 (MQ=255)
                                                                                                                                   cGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGaa         <  1:2232751/140‑1 (MQ=255)
                                                                                                                                    ggATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGaaa        >  1:387988/1‑140 (MQ=255)
                                                                                                                                      aTTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGAAAGc      >  1:383070/1‑140 (MQ=255)
                                                                                                                                      aTTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGAAAGc      >  1:2250638/1‑140 (MQ=255)
                                                                                                                                         tCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGAAAGcccc   <  2:2358312/140‑1 (MQ=255)
                                                                                                                                          cGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGAAAGCCCca  <  2:1970123/140‑1 (MQ=255)
                                                                                                                                          cGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGAAAGCCCca  <  2:1848260/140‑1 (MQ=255)
                                                                                                                                          cGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGAAAGCCCca  <  2:604389/140‑1 (MQ=255)
                                                                                                                                          cGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGAAAGCCCca  <  2:606206/140‑1 (MQ=255)
                                                                                                                                          cGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGAAAGCCCca  <  2:1830502/140‑1 (MQ=255)
                                                                                                                                          cGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGAAAGCCCca  <  2:1276425/140‑1 (MQ=255)
                                                                                                                                          cGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGAAAGCCCca  >  2:870639/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CGTCAGCAAGAAAAGAGGCGGAGGATGGATCAAGCTGACAGCTAAAAAACAAAGCGAGAATGGGTATTACATCAAAGTAGAAGATAACGGTGCCGGCATAGCTCCGGAAAAACGAAAAGATCTCTTATCTGCGGATTTCGATCGCAGCGTCGGATTGAAAAATATCAACCAGCGGCTGAAACATTTTTGCGGAAGCGAATTGAAGATCAGCAGCACTCCGGATGCCGGCACCTCTGTTTCAATGCTCATTCATCTCGCTGAAATAACGGAAAGCCCCA  >  NZ_CP034943/3564308‑3564585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: