Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,668,421 A→T 5.4% M330L (ATG→TTG)  EO946_RS18875 → insulinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,668,4210AT 5.4% 535.0 / 15.3 158M330L (ATG→TTG) EO946_RS18875insulinase family protein
Reads supporting (aligned to +/- strand):  ref base A (61/87);  new base T (4/5);  total (66/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

ACAATTTGCTGCTTGTTTCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTA  >  NZ_CP034943/3668284‑3668559
                                                                                                                                         |                                                                                                                                          
   aTTTGCTGCTTGTTTCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAAtgctg                                                                                                                                       >  1:2277267/1‑140 (MQ=255)
    tttGCTGCTTGTTTCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGGGTTTTGGAAGCTGTCTCACATTTTTCCGCCGATGAGCGGGAGCTCGAACAGGCCAAGCAATTTTTCCGCCATGACCtgctgc                                                                                                                                      >  1:1336288/1‑140 (MQ=255)
            ttGTTTCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTc                                                                                                                              >  2:1081935/1‑140 (MQ=255)
            ttGTTTCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTc                                                                                                                              >  2:1571800/1‑140 (MQ=255)
            ttGTTTCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTc                                                                                                                              >  2:564429/1‑140 (MQ=255)
              gTTTCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGa                                                                                                                            >  1:182434/1‑140 (MQ=255)
              gTTTCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGATGAATCACAATTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGa                                                                                                                            >  1:901508/1‑140 (MQ=255)
                ttCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGAtt                                                                                                                          <  1:1668385/140‑1 (MQ=255)
                         tACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                 <  1:1089050/140‑1 (MQ=255)
                         tACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                 <  1:1894071/140‑1 (MQ=255)
                         tACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                 >  1:166193/1‑140 (MQ=255)
                         tACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                 >  1:427114/1‑140 (MQ=255)
                         tACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                 >  1:843137/1‑140 (MQ=255)
                         tACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                 <  1:472023/140‑1 (MQ=255)
                         tACGAAACAGCTTCACGAGAAATAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCACCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                 <  2:1200996/140‑1 (MQ=255)
                             aaTCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCt                                                                                                             >  2:1141516/1‑140 (MQ=255)
                             aaTCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCt                                                                                                             >  1:1207219/1‑140 (MQ=255)
                              aTCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCtt                                                                                                            <  2:1982183/140‑1 (MQ=255)
                               tCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCttt                                                                                                           <  1:1891545/140‑1 (MQ=255)
                                cAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCtttt                                                                                                          <  2:427114/140‑1 (MQ=255)
                                 aGCTTCACGAGTAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGTCTTTTa                                                                                                         >  2:334084/1‑140 (MQ=255)
                                 aGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                         <  1:801532/140‑1 (MQ=255)
                                 aGCTTCACGAGAAAGAGAGTCCCCCGCGCGTTTTGGAAGCCGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                         <  2:1598629/140‑1 (MQ=255)
                                   cTTCATGAGAAAGACATTCCCCCGCGCGTTTTGGAAGCTGTCTCAGCTTTTCATACAGATGAGCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGc                                                                                                       >  2:216849/1‑140 (MQ=255)
                                   cTTCATGAGAAAGACATTCCCCCGCGCGTTTTGGAAGCTGTCTCAGCTTTTCATACAGATGAGCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGc                                                                                                       >  2:644334/1‑140 (MQ=255)
                                   cTTCATGAGAAAGACATTCCCCCGCGCGTTTTGGAAGCTGTCTCAGCTTTTCATACAGATGAGCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGc                                                                                                       >  2:686392/1‑140 (MQ=255)
                                   cTTCACGAGAAATAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       <  2:1414917/140‑1 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAATTGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       <  2:368853/140‑1 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGACGGGCTTTTAGc                                                                                                       >  1:1531320/1‑140 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       <  2:1476441/140‑1 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       <  2:1006110/140‑1 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       <  2:1868224/140‑1 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       <  2:1036488/140‑1 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       <  1:955319/140‑1 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       <  2:640203/140‑1 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       <  1:893978/140‑1 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       <  2:800390/140‑1 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       >  1:788711/1‑140 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       <  1:1865354/140‑1 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       <  1:1750432/140‑1 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       >  1:608737/1‑140 (MQ=255)
                                   cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATGCGATTCTCCCGAAGGGCTTTTAGc                                                                                                       <  1:1136812/140‑1 (MQ=255)
                                    ttCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGcc                                                                                                      <  2:1739341/140‑1 (MQ=255)
                                     tCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCt                                                                                                     >  2:1097467/1‑140 (MQ=255)
                                      cACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCta                                                                                                    <  1:726927/140‑1 (MQ=255)
                                      cACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCta                                                                                                    >  1:2386643/1‑140 (MQ=255)
                                      cACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCta                                                                                                    <  2:930016/140‑1 (MQ=255)
                                      cACGAGAAAGAGATTCCCCCGCGCGTTTGGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGACCAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCta                                                                                                    <  1:1828364/140‑1 (MQ=255)
                                        cGAGAAAGAGATTCCCCCTCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCtata                                                                                                  >  1:1632052/1‑140 (MQ=255)
                                        cGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                         >  2:298091/1‑133 (MQ=255)
                                        cGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCtata                                                                                                  >  1:1372911/1‑140 (MQ=255)
                                        cGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCtata                                                                                                  >  1:2000656/1‑140 (MQ=255)
                                           gaAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATgg                                                                                               <  2:977115/140‑1 (MQ=255)
                                             aaGACATTCCCCCGCGCGTTTTGGAAGCTGTCTCAGCTTTTCATACAGATGAGCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGCCTATATgggg                                                                                             >  2:1933055/1‑139 (MQ=255)
                                                agaTTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATc                                                                                          <  1:773172/140‑1 (MQ=255)
                                                    tCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgc                                                                                      <  2:2241500/140‑1 (MQ=255)
                                                    tCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTAGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgc                                                                                      <  2:1782063/140‑1 (MQ=255)
                                                      ccccGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgcgc                                                                                    >  1:465047/1‑140 (MQ=255)
                                                                  tGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGcgccgc                                                                                 <  2:756936/131‑1 (MQ=255)
                                                                  tGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGcgccgc                                                                                 >  1:756936/1‑131 (MQ=255)
                                                                   ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                       <  1:972892/140‑1 (MQ=255)
                                                                   ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                       <  1:236199/140‑1 (MQ=255)
                                                                   ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCGAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                       <  1:1317283/140‑1 (MQ=255)
                                                                         tGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAaag                                                                 <  1:690294/140‑1 (MQ=255)
                                                                           tctcACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGACGCTTCTCCTGCACGAaagaa                                                               >  1:2272236/1‑140 (MQ=255)
                                                                                     ttCAGCCGATGAGCGGGAGCTAGAACATGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCACGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                     >  2:698968/1‑140 (MQ=255)
                                                                                     ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCGTAGATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                     >  2:2228183/1‑140 (MQ=255)
                                                                                     ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                     >  2:599835/1‑140 (MQ=255)
                                                                                      tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                    <  2:1574240/140‑1 (MQ=255)
                                                                                      tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCCTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                    <  1:1081935/140‑1 (MQ=255)
                                                                                         gCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAAGGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATgg                                                 <  1:349789/140‑1 (MQ=255)
                                                                                             aTGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCAtc                                             >  1:1134378/1‑140 (MQ=255)
                                                                                                 gccggAGCGAGAACAGCCAAAGCATTTTTTCCGCAATGAAATGCTTCTTCAAGTCGATTCTCCCGAAGGTCTTTGAGCCTATATGGGAATCCTGCGCCGCTTCTGCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                         <  2:1428525/137‑1 (MQ=255)
                                                                                                 gCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                         <  2:1488322/140‑1 (MQ=255)
                                                                                                 gCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                         <  2:2039139/140‑1 (MQ=255)
                                                                                                 gCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                         <  2:133235/140‑1 (MQ=255)
                                                                                                 gCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGCCTATATGGGGGTCCTGCGCCGCTTCTCCTGCACGAAAGAAGCCTTGCTGGATGGCATCTCAg                                         <  1:644334/140‑1 (MQ=255)
                                                                                                 gCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGCCTATATGGGGGTCCTGCGCCGCTTCTCCTGCACGAAAGAAGCCTTGCTGGATGGCATCTCAg                                         <  1:216849/140‑1 (MQ=255)
                                                                                                    ggcgCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTATCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAATACTTGCTGGATGGCATCTCAGCgg                                      >  2:57512/4‑140 (MQ=255)
                                                                                                    ggAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCgg                                      >  2:211846/1‑140 (MQ=255)
                                                                                                           gAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGCCTATATGGGGGTCCTGCGCCGCTTCTCCTGCACGAAAGAAGCCTTGCTGGATGGCATCTCAGCGGTTACATg                               <  1:1933055/140‑1 (MQ=255)
                                                                                                                ggCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCcgcg                          <  1:1789650/140‑1 (MQ=255)
                                                                                                                ggCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCcgcg                          <  2:2277267/140‑1 (MQ=255)
                                                                                                                 gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                         >  1:432810/1‑140 (MQ=255)
                                                                                                                 gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                         >  1:772430/1‑140 (MQ=255)
                                                                                                                 gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                         >  2:1675162/1‑140 (MQ=255)
                                                                                                                 gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                         >  1:1146765/1‑140 (MQ=255)
                                                                                                                 gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                         >  2:1708908/1‑140 (MQ=255)
                                                                                                                 gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                         >  1:1597834/1‑140 (MQ=255)
                                                                                                                 gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                         >  1:981642/1‑140 (MQ=255)
                                                                                                                 gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                         >  1:1836251/1‑140 (MQ=255)
                                                                                                                 gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                         >  2:2232349/1‑140 (MQ=255)
                                                                                                                 gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATAGGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                         >  1:557136/1‑140 (MQ=255)
                                                                                                                         aattttCCGCCCTCACAGGCTGCTACAATGCGATTCTCCCGCAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCGCCTGCACGAAAGCAGACTTGCTGGAGGGCATCTCAGCGGTTACGTGCCGCGATGTATTGc                 <  2:1467951/138‑1 (MQ=255)
                                                                                                                           ttttCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCaa               >  2:859837/1‑140 (MQ=255)
                                                                                                                                cGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCAt          <  2:432810/140‑1 (MQ=255)
                                                                                                                                  cAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATaa        <  2:1921534/140‑1 (MQ=255)
                                                                                                                                  cAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATaa        <  2:2146552/140‑1 (MQ=255)
                                                                                                                                  cAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATaa        <  2:2129062/140‑1 (MQ=255)
                                                                                                                                       aaaTGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACt   <  2:788711/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
ACAATTTGCTGCTTGTTTCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTA  >  NZ_CP034943/3668284‑3668559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: