Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,668,429 T→G 5.0% L332L (CTT→CTG EO946_RS18875 → insulinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,668,4290TG 5.0% 508.9 / 11.0 160L332L (CTT→CTGEO946_RS18875insulinase family protein
Reads supporting (aligned to +/- strand):  ref base T (61/90);  new base G (4/4);  total (65/95)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 163 total aligned reads displayed.

TTGTTTCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATA  >  NZ_CP034943/3668296‑3668568
                                                                                                                                     |                                                                                                                                           
ttGTTTCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTc                                                                                                                                       >  2:1081935/1‑140 (MQ=255)
  gTTTCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGATCGGGAGCTAGAACAGGCAAAGCAATTTTTCAGCAATGAAATGCTGCTTCAATTcta                                                                                                                                     >  1:842650/1‑138 (MQ=255)
    ttCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGAtt                                                                                                                                   <  1:1668385/140‑1 (MQ=255)
             tACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                          <  1:1089050/140‑1 (MQ=255)
             tACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                          >  1:427114/1‑140 (MQ=255)
             tACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                          <  1:762584/140‑1 (MQ=255)
             tACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                          <  1:1894071/140‑1 (MQ=255)
             tACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                          >  2:1100882/1‑140 (MQ=255)
             tACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                          <  2:787695/140‑1 (MQ=255)
             tACGAAACAGCTTCACGAGAAATAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCACCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAg                                                                                                                          <  2:1200996/140‑1 (MQ=255)
                 aaTCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCt                                                                                                                      >  1:936635/1‑140 (MQ=255)
                 aaTCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCt                                                                                                                      >  1:1207219/1‑140 (MQ=255)
                  aTCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCtt                                                                                                                     >  2:65741/1‑140 (MQ=255)
                   tCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCttt                                                                                                                    <  1:735176/140‑1 (MQ=255)
                   tCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCttt                                                                                                                    <  1:1713292/140‑1 (MQ=255)
                   tCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCttt                                                                                                                    <  1:1891545/140‑1 (MQ=255)
                    cAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCtttt                                                                                                                   <  2:458126/140‑1 (MQ=255)
                    cAGCTTCACGAGAAAGAGAGTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCtttt                                                                                                                   <  2:166193/140‑1 (MQ=255)
                     aGCTTCACGAGTAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGTCTTTTa                                                                                                                  >  2:334084/1‑140 (MQ=255)
                     aGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                                  <  1:801532/140‑1 (MQ=255)
                     aGCTTCACGAGAAAGAGATTCCCCCGCGCGTGTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                                  <  1:782466/140‑1 (MQ=255)
                       cTTCATGAGAAAGACATTCCCCCGCGCGTTTTGGAAGCTGTCTCAGCTTTTCATACAGATGAGCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGc                                                                                                                >  2:644334/1‑140 (MQ=255)
                       cTTCATGAGAAAGACATTCCCCCGCGCGTTTTGGAAGCTGTCTCAGCTTTTCATACAGATGAGCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGc                                                                                                                >  2:216849/1‑140 (MQ=255)
                       cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGACGGGCTTTTAGc                                                                                                                >  1:1531320/1‑140 (MQ=255)
                       cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                <  1:893978/140‑1 (MQ=255)
                       cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                <  2:1476441/140‑1 (MQ=255)
                       cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                <  2:1916259/140‑1 (MQ=255)
                       cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                <  2:800390/140‑1 (MQ=255)
                       cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                >  1:608737/1‑140 (MQ=255)
                       cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                <  2:1006110/140‑1 (MQ=255)
                       cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                <  1:1637877/140‑1 (MQ=255)
                       cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                <  1:1750432/140‑1 (MQ=255)
                       cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATGCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                <  1:1136812/140‑1 (MQ=255)
                          cACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCta                                                                                                             >  1:2386643/1‑140 (MQ=255)
                          cACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCta                                                                                                             <  2:930016/140‑1 (MQ=255)
                          cACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCta                                                                                                             <  2:417095/140‑1 (MQ=255)
                          cACGAGAAAGAGATTCCCCCGCGCGTTTGGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGACCAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCta                                                                                                             <  1:1828364/140‑1 (MQ=255)
                            cGAGAAAGAGATTCCCCCTCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCtata                                                                                                           >  1:1632052/1‑140 (MQ=255)
                            cGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                                  <  1:298091/133‑1 (MQ=255)
                            cGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCtata                                                                                                           >  1:1372911/1‑140 (MQ=255)
                            cGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCtata                                                                                                           >  1:2000656/1‑140 (MQ=255)
                               gaAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATgg                                                                                                        <  2:977115/140‑1 (MQ=255)
                                 aaGACATTCCCCCGCGCGTTTTGGAAGCTGTCTCAGCTTTTCATACAGATGAGCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGCCTATATgggg                                                                                                      >  2:1933055/1‑139 (MQ=255)
                                        tCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgc                                                                                               <  2:268375/140‑1 (MQ=255)
                                        tCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgc                                                                                               <  2:2241500/140‑1 (MQ=255)
                                        tCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgc                                                                                               <  2:195245/140‑1 (MQ=255)
                                        tCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTAGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgc                                                                                               <  2:1782063/140‑1 (MQ=255)
                                          ccccGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgcgc                                                                                             >  1:465047/1‑140 (MQ=255)
                                                      tGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGcgccgc                                                                                          <  2:756936/131‑1 (MQ=255)
                                                       ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                                <  1:433732/140‑1 (MQ=255)
                                                       ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                                <  1:862244/140‑1 (MQ=255)
                                                       ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                                <  1:972892/140‑1 (MQ=255)
                                                       ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCGAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                                <  1:1317283/140‑1 (MQ=255)
                                                             tGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAaag                                                                          <  1:690294/140‑1 (MQ=255)
                                                              gTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAaaga                                                                         >  2:1553791/1‑140 (MQ=255)
                                                               tctcACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGACGCTTCTCCTGCACGAaagaa                                                                        >  1:2272236/1‑140 (MQ=255)
                                                                         ttCAGCCGATGAGCGGGAGCTAGAACATGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCACGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                              >  2:698968/1‑140 (MQ=255)
                                                                         ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                              >  2:599835/1‑140 (MQ=255)
                                                                         ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                              >  1:1826524/1‑140 (MQ=255)
                                                                         ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                              >  2:602975/1‑140 (MQ=255)
                                                                         ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                              >  1:1869846/1‑140 (MQ=255)
                                                                         ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                              >  2:2228187/1‑140 (MQ=255)
                                                                         ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                              >  1:1859314/1‑140 (MQ=255)
                                                                          tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                             <  1:564429/140‑1 (MQ=255)
                                                                          tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                             <  2:362800/140‑1 (MQ=255)
                                                                          tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                             <  1:1571800/140‑1 (MQ=255)
                                                                          tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                             <  2:1574240/140‑1 (MQ=255)
                                                                          tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCCTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                             <  1:1081935/140‑1 (MQ=255)
                                                                                 aTGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCAtc                                                      >  1:1134378/1‑140 (MQ=255)
                                                                                 aTGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCAtc                                                      >  1:501061/1‑140 (MQ=255)
                                                                                 aTGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCAtc                                                      >  1:2094231/1‑140 (MQ=255)
                                                                                     gccggAGCGAGAACAGCCAAAGCATTTTTTCCGCAATGAAATGCTTCTTCAAGTCGATTCTCCCGAAGGTCTTTGAGCCTATATGGGAATCCTGCGCCGCTTCTGCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                                  <  2:1428525/137‑1 (MQ=255)
                                                                                     gCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                                  <  2:133235/140‑1 (MQ=255)
                                                                                     gCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                                  <  2:2039139/140‑1 (MQ=255)
                                                                                     gCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCATTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                                  <  2:1472182/140‑1 (MQ=255)
                                                                                     gCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGCCTATATGGGGGTCCTGCGCCGCTTCTCCTGCACGAAAGAAGCCTTGCTGGATGGCATCTCAg                                                  <  1:216849/140‑1 (MQ=255)
                                                                                     gCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGCCTATATGGGGGTCCTGCGCCGCTTCTCCTGCACGAAAGAAGCCTTGCTGGATGGCATCTCAg                                                  <  1:686392/140‑1 (MQ=255)
                                                                                        ggcgCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTATCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAATACTTGCTGGATGGCATCTCAGCgg                                               >  2:57512/4‑140 (MQ=255)
                                                                                        ggAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCgg                                               >  2:211846/1‑140 (MQ=255)
                                                                                            cTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTAc                                           >  2:1709695/1‑140 (MQ=255)
                                                                                                  catgCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCcg                                     <  1:2318116/137‑1 (MQ=255)
                                                                                                    ggCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCcgcg                                   <  1:1789650/140‑1 (MQ=255)
                                                                                                     gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                  >  1:981642/1‑140 (MQ=255)
                                                                                                     gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                  >  2:2232349/1‑140 (MQ=255)
                                                                                                     gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                  >  1:1146765/1‑140 (MQ=255)
                                                                                                     gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                  >  2:1675162/1‑140 (MQ=255)
                                                                                                     gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                  >  1:772430/1‑140 (MQ=255)
                                                                                                               ttttCCGCACTGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCaa                        <  2:1059037/140‑1 (MQ=255)
                                                                                                               ttttCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCaa                        <  2:1864416/140‑1 (MQ=255)
                                                                                                               ttttCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCaa                        >  2:859837/1‑140 (MQ=255)
                                                                                                               ttttCCGCAAGGAAAGGCTGCTTCCAGTCGAGTCGCCCGCAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCaa                        <  2:1351997/140‑1 (MQ=255)
                                                                                                                    cGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCAt                   <  2:432810/140‑1 (MQ=255)
                                                                                                                      cAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATaa                 <  2:2146552/140‑1 (MQ=255)
                                                                                                                      cAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATaa                 <  2:2129062/140‑1 (MQ=255)
                                                                                                                       aaTGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAAc                <  2:1146765/140‑1 (MQ=255)
                                                                                                                            aaTGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTa           <  2:1597834/140‑1 (MQ=255)
                                                                                                                                 tgctTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTattaat      >  2:2043053/1‑140 (MQ=255)
                                                                                                                                  gctTCAATTCGAGTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTattaatt     <  1:1845758/140‑1 (MQ=255)
                                                                                                                                   ctTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTa           <  2:230905/133‑1 (MQ=255)
                                                                                                                                     tCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATtata  >  1:234290/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                           
TTGTTTCAATATGTACGAATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATA  >  NZ_CP034943/3668296‑3668568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: