Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,668,450 G→T 5.4% G339G (GGG→GGT EO946_RS18875 → insulinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,668,4500GT 5.4% 547.2 / 12.3 179G339G (GGG→GGTEO946_RS18875insulinase family protein
Reads supporting (aligned to +/- strand):  ref base G (57/110);  new base T (5/7);  total (62/117)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

AATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATGTAGTAAG  >  NZ_CP034943/3668313‑3668587
                                                                                                                                         |                                                                                                                                         
aaTCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCt                                                                                                                                         >  1:1207219/1‑140 (MQ=255)
aaTCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCt                                                                                                                                         >  2:1141516/1‑140 (MQ=255)
 aTCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCtt                                                                                                                                        >  2:65741/1‑140 (MQ=255)
  tCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCttt                                                                                                                                       <  1:735176/140‑1 (MQ=255)
  tCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCttt                                                                                                                                       <  1:2215052/140‑1 (MQ=255)
  tCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCttt                                                                                                                                       <  1:1891545/140‑1 (MQ=255)
   cAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCtttt                                                                                                                                      <  2:458126/140‑1 (MQ=255)
   cAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCtttt                                                                                                                                      <  2:427114/140‑1 (MQ=255)
   cAGCTTCACGAGAAAGAGAGTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCtttt                                                                                                                                      <  2:166193/140‑1 (MQ=255)
    aGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                                                     <  1:801532/140‑1 (MQ=255)
    aGCTTCACGAGAAAGAGATTCCCCCGCGCGTGTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                                                     <  1:782466/140‑1 (MQ=255)
    aGCGTCACGAGAAAGAGAGTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                                                     <  2:930424/140‑1 (MQ=255)
      cTTCATGAGAAAGACATTCCCCCGCGCGTTTTGGAAGCTGTCTCAGCTTTTCATACAGATGAGCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGc                                                                                                                                   >  2:216849/1‑140 (MQ=255)
      cTTCACGAGATAGAGATTCCCGCGAGCGTTTTGGCAGCTGTCTCACATTTTTCAGCAGATGAGCGGTAGCTAGAACAGGCAAATCAATTTTTCAGCAATGAAATTCTGCTTCAATTCGATTATCCCTAATGTCTTTTAGc                                                                                                                                   <  2:2232931/140‑1 (MQ=255)
      cTTCACGAGAAATAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                   <  2:1414917/140‑1 (MQ=255)
      cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAATTGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                   <  2:368853/140‑1 (MQ=255)
      cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGACGGGCTTTTAGc                                                                                                                                   >  1:1531320/1‑140 (MQ=255)
      cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                   <  1:1750432/140‑1 (MQ=255)
      cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                   <  1:955319/140‑1 (MQ=255)
      cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                   <  2:1006110/140‑1 (MQ=255)
      cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                   <  2:1868224/140‑1 (MQ=255)
      cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                   <  2:640203/140‑1 (MQ=255)
      cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                   <  1:1865354/140‑1 (MQ=255)
      cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                   <  2:800390/140‑1 (MQ=255)
      cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATGCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                   <  1:1136812/140‑1 (MQ=255)
         cACGAGAAAGAGATTCCCCCGCGCGTTTGGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGACCAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCta                                                                                                                                <  1:1828364/140‑1 (MQ=255)
           cGAGAAAGAGATTCCCCCTCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCtata                                                                                                                              >  1:1632052/1‑140 (MQ=255)
                       tCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgc                                                                                                                  <  2:268375/140‑1 (MQ=255)
                       tCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgc                                                                                                                  <  2:195245/140‑1 (MQ=255)
                       tCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTAGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgc                                                                                                                  <  2:1782063/140‑1 (MQ=255)
                                     tGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGcgccgc                                                                                                             <  2:756936/131‑1 (MQ=255)
                                      ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                                                   <  1:433732/140‑1 (MQ=255)
                                      ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                                                   <  1:236199/140‑1 (MQ=255)
                                      ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCGAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                                                   <  1:1317283/140‑1 (MQ=255)
                                            tGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAaag                                                                                             <  1:690294/140‑1 (MQ=255)
                                             gTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAaaga                                                                                            >  2:1553791/1‑140 (MQ=255)
                                              tctcACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGACGCTTCTCCTGCACGAaagaa                                                                                           >  1:2272236/1‑140 (MQ=255)
                                                tcACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAagaaga                                                                                         <  2:2045086/140‑1 (MQ=255)
                                                        ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                                                 >  2:599835/1‑140 (MQ=255)
                                                        ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                                                 >  1:1869846/1‑140 (MQ=255)
                                                        ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                                                 >  2:2228187/1‑140 (MQ=255)
                                                         tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                                                <  2:706591/140‑1 (MQ=255)
                                                         tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                                                <  1:1100882/140‑1 (MQ=255)
                                                         tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCCTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                                                <  1:1081935/140‑1 (MQ=255)
                                                            gCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAAGGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATgg                                                                             <  1:349789/140‑1 (MQ=255)
                                                                aTGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCAtc                                                                         >  1:501061/1‑140 (MQ=255)
                                                                aTGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCAtc                                                                         >  1:133235/1‑140 (MQ=255)
                                                                    gCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                                                     <  2:2039139/140‑1 (MQ=255)
                                                                    gCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                                                     <  2:1488322/140‑1 (MQ=255)
                                                                    gCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                                                     <  2:133235/140‑1 (MQ=255)
                                                                    gCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCATTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                                                     <  2:1472182/140‑1 (MQ=255)
                                                                    gCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGCCTATATGGGGGTCCTGCGCCGCTTCTCCTGCACGAAAGAAGCCTTGCTGGATGGCATCTCAg                                                                     <  1:644334/140‑1 (MQ=255)
                                                                       ggcgCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTATCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAATACTTGCTGGATGGCATCTCAGCgg                                                                  >  2:57512/4‑140 (MQ=255)
                                                                       ggAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCgg                                                                  >  2:211846/1‑140 (MQ=255)
                                                                           cTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTAc                                                              >  2:1709695/1‑140 (MQ=255)
                                                                                 catgCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCcg                                                        <  1:2318116/137‑1 (MQ=255)
                                                                                   ggCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCcgcg                                                      <  1:1789650/140‑1 (MQ=255)
                                                                                   ggCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCcgcg                                                      <  2:2277267/140‑1 (MQ=255)
                                                                                    gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                                     >  1:1146765/1‑140 (MQ=255)
                                                                                    gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                                     >  1:432810/1‑140 (MQ=255)
                                                                                    gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                                     >  1:981642/1‑140 (MQ=255)
                                                                                    gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                                     >  1:772430/1‑140 (MQ=255)
                                                                                    gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                                     >  2:1675162/1‑140 (MQ=255)
                                                                                    gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                                     >  2:1708908/1‑140 (MQ=255)
                                                                                    gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                                     >  1:1597834/1‑140 (MQ=255)
                                                                                    gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATAGGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                                     >  1:557136/1‑140 (MQ=255)
                                                                                              ttttCCGCACTGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCaa                                           <  2:1059037/140‑1 (MQ=255)
                                                                                              ttttCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCaa                                           >  2:859837/1‑140 (MQ=255)
                                                                                              ttttCCGCAAGGAAAGGCTGCTTCCAGTCGAGTCGCCCGCAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCaa                                           <  2:1351997/140‑1 (MQ=255)
                                                                                                     cAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATaa                                    <  2:1921534/140‑1 (MQ=255)
                                                                                                     cAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATaa                                    <  2:2129062/140‑1 (MQ=255)
                                                                                                     cAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATaa                                    <  2:2146552/140‑1 (MQ=255)
                                                                                                           aaTGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTa                              <  2:1597834/140‑1 (MQ=255)
                                                                                                                tgctTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTattaat                         >  2:127751/1‑140 (MQ=255)
                                                                                                                 gctTCAATTCGAGTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTattaatt                        <  1:1845758/140‑1 (MQ=255)
                                                                                                                    tCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGAGTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATtata                     >  1:1690146/1‑140 (MQ=255)
                                                                                                                    tCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATtata                     >  1:2054927/1‑140 (MQ=255)
                                                                                                                     cAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTatat                    <  1:693654/140‑1 (MQ=255)
                                                                                                                       aTTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCg                  >  1:1993929/1‑140 (MQ=255)
                                                                                                                       aTTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTAATCGGTTACGTGCCGCGATGTATTTCAATTCATAACGACTATTAATTATATct                  >  1:865661/1‑139 (MQ=255)
                                                                                                                        ttCGATTCTCCCGAAGGGCTTTTAGCCTAGATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTACTTATATCgg                 <  2:358578/140‑1 (MQ=255)
                                                                                                                        ttCGATTCTCCCGAAGGGCATTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCGGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTCTTAATTATATCgg                 <  2:1771878/140‑1 (MQ=255)
                                                                                                                          cGATTCTCCCGAAGTGCTTTTAGACTATATGGGAATCCTGCGCCGCTTCTCCTGCTCGCAAGTAGACTTTCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTTTTGAAATTCATAACGACTATTAATTATATGGGAg               <  2:2325624/140‑1 (MQ=255)
                                                                                                                          cGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAg               <  2:68857/140‑1 (MQ=255)
                                                                                                                            aTTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCt             >  2:1437060/1‑140 (MQ=255)
                                                                                                                             ttCTCCCGATGGTCTTTTAGCCTATATGGGAATAATGCGCCGATTCTCGTGAACGAAAGAAGTCTTGCTGGATTGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGTATATTAATTATATCGGAGCTc            <  1:57512/140‑1 (MQ=255)
                                                                                                                             ttCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTTCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTc            <  2:1023824/140‑1 (MQ=255)
                                                                                                                             ttCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTc            <  2:785427/140‑1 (MQ=255)
                                                                                                                             ttCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTc            <  1:831925/140‑1 (MQ=255)
                                                                                                                             ttCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTc            <  1:211846/140‑1 (MQ=255)
                                                                                                                              tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa           <  2:2000656/140‑1 (MQ=255)
                                                                                                                              tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa           <  1:291020/140‑1 (MQ=255)
                                                                                                                              tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa           <  2:317935/140‑1 (MQ=255)
                                                                                                                              tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa           <  1:915105/140‑1 (MQ=255)
                                                                                                                              tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa           <  2:1955228/140‑1 (MQ=255)
                                                                                                                              tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa           <  2:1161077/140‑1 (MQ=255)
                                                                                                                                tcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATg         <  1:1427516/140‑1 (MQ=255)
                                                                                                                                     aaGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATgtagta    <  1:859837/140‑1 (MQ=255)
                                                                                                                                       gggCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGTGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATGTAGTAAg  <  2:1618525/140‑1 (MQ=255)
                                                                                                                                       gggCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATGTAGTAAg  <  1:1709695/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
AATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATGTAGTAAG  >  NZ_CP034943/3668313‑3668587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: