Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,668,453 T→A 5.1% L340L (CTT→CTA EO946_RS18875 → insulinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,668,4530TA 5.1% 564.8 / 14.7 172L340L (CTT→CTAEO946_RS18875insulinase family protein
Reads supporting (aligned to +/- strand):  ref base T (55/108);  new base A (4/5);  total (59/113)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 187 total aligned reads displayed.

ATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATGTAGTAAGGGGAT  >  NZ_CP034943/3668314‑3668592
                                                                                                                                           |                                                                                                                                           
aTCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCtt                                                                                                                                             <  2:1982183/140‑1 (MQ=255)
 tCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCttt                                                                                                                                            <  1:2215052/140‑1 (MQ=255)
 tCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCttt                                                                                                                                            <  1:1891545/140‑1 (MQ=255)
 tCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCttt                                                                                                                                            <  1:735176/140‑1 (MQ=255)
  cAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCtttt                                                                                                                                           <  2:458126/140‑1 (MQ=255)
   aGCTTCACGAGAAAGAGATTCCCCCGCGCGTGTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                                                          <  1:782466/140‑1 (MQ=255)
   aGCTTCACGAGAAAGAGAGTCCCCCGCGCGTTTTGGAAGCCGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                                                          <  2:1598629/140‑1 (MQ=255)
   aGCGTCACGAGAAAGAGAGTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                                                          <  2:930424/140‑1 (MQ=255)
     cTTCATGAGAAAGACATTCCCCCGCGCGTTTTGGAAGCTGTCTCAGCTTTTCATACAGATGAGCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGc                                                                                                                                        >  2:216849/1‑140 (MQ=255)
     cTTCATGAGAAAGACATTCCCCCGCGCGTTTTGGAAGCTGTCTCAGCTTTTCATACAGATGAGCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGc                                                                                                                                        >  2:686392/1‑140 (MQ=255)
     cTTCATGAGAAAGACATTCCCCCGCGCGTTTTGGAAGCTGTCTCAGCTTTTCATACAGATGAGCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGc                                                                                                                                        >  2:644334/1‑140 (MQ=255)
     cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGACGGGCTTTTAGc                                                                                                                                        >  1:1531320/1‑140 (MQ=255)
     cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                        <  2:1916259/140‑1 (MQ=255)
     cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                        >  1:608737/1‑140 (MQ=255)
     cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                        <  1:1750432/140‑1 (MQ=255)
     cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                        <  1:1637877/140‑1 (MQ=255)
     cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                        >  1:788711/1‑140 (MQ=255)
     cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                        <  2:1036488/140‑1 (MQ=255)
     cTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATGCGATTCTCCCGAAGGGCTTTTAGc                                                                                                                                        <  1:1136812/140‑1 (MQ=255)
      ttCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGcc                                                                                                                                       <  2:1739341/140‑1 (MQ=255)
       tCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCt                                                                                                                                      >  2:1097467/1‑140 (MQ=255)
        cACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCta                                                                                                                                     <  2:417095/140‑1 (MQ=255)
        cACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCta                                                                                                                                     >  1:2386643/1‑140 (MQ=255)
        cACGAGAAAGAGATTCCCCCGCGCGTTTGGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGACCAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCta                                                                                                                                     <  1:1828364/140‑1 (MQ=255)
          cGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                                                          >  2:298091/1‑133 (MQ=255)
          cGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTa                                                                                                                                          <  1:298091/133‑1 (MQ=255)
               aaGACATTCCCCCGCGCGTTTTGGAAGCTGTCTCAGCTTTTCATACAGATGAGCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGCCTATATgggg                                                                                                                              >  2:1933055/1‑139 (MQ=255)
                      tCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgc                                                                                                                       <  2:2241500/140‑1 (MQ=255)
                      tCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgc                                                                                                                       <  2:268375/140‑1 (MQ=255)
                      tCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTAGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTgc                                                                                                                       <  2:1782063/140‑1 (MQ=255)
                                     ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                                                        <  1:972892/140‑1 (MQ=255)
                                     ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                                                        <  1:433732/140‑1 (MQ=255)
                                     ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                                                        <  2:2119227/140‑1 (MQ=255)
                                     ggAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCa                                                                                                        <  2:585337/140‑1 (MQ=255)
                                                       ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCGTAGATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                                                      >  2:2228183/1‑140 (MQ=255)
                                                       ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                                                      >  1:1869846/1‑140 (MQ=255)
                                                       ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                                                      >  1:1859314/1‑140 (MQ=255)
                                                       ttCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTgg                                                                                      >  2:602975/1‑140 (MQ=255)
                                                        tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATCTGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                                                     <  1:992520/140‑1 (MQ=255)
                                                        tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                                                     <  2:1574240/140‑1 (MQ=255)
                                                        tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                                                     <  2:362800/140‑1 (MQ=255)
                                                        tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                                                     <  2:706591/140‑1 (MQ=255)
                                                        tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                                                     <  1:1100882/140‑1 (MQ=255)
                                                        tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                                                     <  1:564429/140‑1 (MQ=255)
                                                        tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                                                     <  1:1571800/140‑1 (MQ=255)
                                                        tCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCCTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGa                                                                                     <  1:1081935/140‑1 (MQ=255)
                                                               aTGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCAtc                                                                              >  1:133235/1‑140 (MQ=255)
                                                               aTGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCAtc                                                                              >  1:501061/1‑140 (MQ=255)
                                                                   gccggAGCGAGAACAGCCAAAGCATTTTTTCCGCAATGAAATGCTTCTTCAAGTCGATTCTCCCGAAGGTCTTTGAGCCTATATGGGAATCCTGCGCCGCTTCTGCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                                                          <  2:1428525/137‑1 (MQ=255)
                                                                   gCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                                                          <  2:1488322/140‑1 (MQ=255)
                                                                   gCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCATTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAg                                                                          <  2:1472182/140‑1 (MQ=255)
                                                                   gCGGGAACTAGAACAGGCAAAGCAGTTTTTACGCAATGAATTGCTGCTGCAATTCGATTCTCCCGAAGGTCTATTGGCCTATATGGGGGTCCTGCGCCGCTTCTCCTGCACGAAAGAAGCCTTGCTGGATGGCATCTCAg                                                                          <  1:686392/140‑1 (MQ=255)
                                                                                catgCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCcg                                                             <  1:2318116/137‑1 (MQ=255)
                                                                                  ggCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCcgcg                                                           <  1:1789650/140‑1 (MQ=255)
                                                                                   gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                                          >  1:1146765/1‑140 (MQ=255)
                                                                                   gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                                          >  2:1708908/1‑140 (MQ=255)
                                                                                   gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                                          >  2:2232349/1‑140 (MQ=255)
                                                                                   gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                                          >  2:831925/1‑140 (MQ=255)
                                                                                   gCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGa                                                          >  1:772430/1‑140 (MQ=255)
                                                                                             ttttCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCaa                                                <  2:1864416/140‑1 (MQ=255)
                                                                                                  cGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCAt                                           <  2:432810/140‑1 (MQ=255)
                                                                                                    cAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATaa                                         <  2:2146552/140‑1 (MQ=255)
                                                                                                         aaaTGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACt                                    <  2:788711/140‑1 (MQ=255)
                                                                                                               tgctTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTattaat                              >  2:2043053/1‑140 (MQ=255)
                                                                                                               tgctTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTattaat                              >  2:127751/1‑140 (MQ=255)
                                                                                                                gctTCAATTCGAGTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTattaatt                             <  1:1845758/140‑1 (MQ=255)
                                                                                                                 ctTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTa                                   <  2:230905/133‑1 (MQ=255)
                                                                                                                 ctTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTa                                   >  1:230905/1‑133 (MQ=255)
                                                                                                                   tCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATtata                          >  1:2054927/1‑140 (MQ=255)
                                                                                                                    cAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTatat                         <  1:693654/140‑1 (MQ=255)
                                                                                                                      aTTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCg                       >  1:1993929/1‑140 (MQ=255)
                                                                                                                      aTTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTAATCGGTTACGTGCCGCGATGTATTTCAATTCATAACGACTATTAATTATATct                       >  1:865661/1‑139 (MQ=255)
                                                                                                                       ttCGATTCTCCCGAAGGGCTTTTAGCCTAGATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTACTTATATCgg                      <  2:358578/140‑1 (MQ=255)
                                                                                                                       ttCGATTCTCCCGAAGGGCATTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCGGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTCTTAATTATATCgg                      <  2:1771878/140‑1 (MQ=255)
                                                                                                                       ttCGATTCGCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCgg                      <  2:1336288/140‑1 (MQ=255)
                                                                                                                         cGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAg                    <  2:68857/140‑1 (MQ=255)
                                                                                                                           aTTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCt                  >  2:1437060/1‑140 (MQ=255)
                                                                                                                            ttCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTTCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTc                 <  2:1023824/140‑1 (MQ=255)
                                                                                                                            ttCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTc                 <  1:211846/140‑1 (MQ=255)
                                                                                                                            ttCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTc                 <  2:1671081/140‑1 (MQ=255)
                                                                                                                            ttCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTc                 <  2:779744/140‑1 (MQ=255)
                                                                                                                             tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa                <  2:1632052/140‑1 (MQ=255)
                                                                                                                             tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa                <  2:1161077/140‑1 (MQ=255)
                                                                                                                             tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa                <  1:814852/140‑1 (MQ=255)
                                                                                                                             tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa                <  2:1372911/140‑1 (MQ=255)
                                                                                                                             tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa                <  2:317935/140‑1 (MQ=255)
                                                                                                                             tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa                <  1:396620/140‑1 (MQ=255)
                                                                                                                             tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa                <  2:1955228/140‑1 (MQ=255)
                                                                                                                             tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa                <  1:915105/140‑1 (MQ=255)
                                                                                                                             tctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCa                <  2:2002421/140‑1 (MQ=255)
                                                                                                                              ctcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCAt               <  2:168671/140‑1 (MQ=255)
                                                                                                                               tcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATg              <  2:2063917/140‑1 (MQ=255)
                                                                                                                               tcCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATg              <  1:1427516/140‑1 (MQ=255)
                                                                                                                                 ccGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGTGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATgta            >  1:1618525/1‑140 (MQ=255)
                                                                                                                                 ccGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATgta            >  1:1219818/1‑140 (MQ=255)
                                                                                                                                    aaGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATgtagta         <  1:859837/140‑1 (MQ=255)
                                                                                                                                      gggCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATGTAGTAAg       <  1:1709695/140‑1 (MQ=255)
                                                                                                                                      gggCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATGTAGTAAg       <  1:53453/140‑1 (MQ=255)
                                                                                                                                          ttttAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATGTAGTAAGGGGa   <  2:901508/140‑1 (MQ=255)
                                                                                                                                           tttAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATGTAGTAAGGGGAt  >  1:2356046/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ATCAGCTTCACGAGAAAGAGATTCCCCCGCGCGTTTTGGAAGCTGTCTCACATTTTTCAGCCGATGAGCGGGAGCTAGAACAGGCAAAGCAATTTTTCCGCAATGAAATGCTGCTTCAATTCGATTCTCCCGAAGGGCTTTTAGCCTATATGGGAATCCTGCGCCGCTTCTCCTGCACGAAAGAAGACTTGCTGGATGGCATCTCAGCGGTTACGTGCCGCGATGTATTGCAATTCATAACGACTATTAATTATATCGGAGCTCATGTAGTAAGGGGAT  >  NZ_CP034943/3668314‑3668592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: