BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,746,846	C→G	5.4%	G74G (GGG→GGC)	EO946_RS19275 ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,746,846	0	C	G	5.4%	676.9 / 20.9	204	G74G (GGG→GGC)	EO946_RS19275	galactokinase
Reads supporting (aligned to +/- strand): ref base C (70/123); new base G (7/4); total (77/127)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 209 total aligned reads displayed.

ATTCCCTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCA  >  NZ_CP034943/3746708‑3746985

atTCCCTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATcc                                                                                                                                            >  2:1616418/1‑140 (MQ=255)

atTCCCTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCGTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATcc                                                                                                                                            >  2:139811/1‑140 (MQ=255)

  tCCCTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCt                                                                                                                                          >  2:1859530/1‑140 (MQ=255)

  tCCCTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCt                                                                                                                                          >  1:1342306/1‑140 (MQ=255)

  tCCCTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCt                                                                                                                                          >  2:291582/1‑140 (MQ=255)

  tCCCTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCt                                                                                                                                          >  2:430488/1‑140 (MQ=255)

   cccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTa                                                                                                                                         <  1:1102439/140‑1 (MQ=255)

   cccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTa                                                                                                                                         <  1:729229/140‑1 (MQ=255)

   cccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTa                                                                                                                                         <  1:645918/140‑1 (MQ=255)

   cccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTa                                                                                                                                         >  1:57858/1‑140 (MQ=255)

   cccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTa                                                                                                                                         <  1:255508/140‑1 (MQ=255)

   cccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTa                                                                                                                                         <  1:167791/140‑1 (MQ=255)

    ccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAc                                                                                                                                        <  1:986619/140‑1 (MQ=255)

    ccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAc                                                                                                                                        <  1:700510/140‑1 (MQ=255)

    ccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAc                                                                                                                                        <  1:675412/140‑1 (MQ=255)

    ccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAc                                                                                                                                        <  1:52117/140‑1 (MQ=255)

    ccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAc                                                                                                                                        <  1:454076/140‑1 (MQ=255)

      tGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCCTTCCTTGATCCCTACGt                                                                                                                                      >  2:2000421/1‑140 (MQ=255)

       gAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTc                                                                                                                                     >  1:2416497/1‑140 (MQ=255)

       gAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTc                                                                                                                                     >  1:2204782/1‑140 (MQ=255)

              caATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAc                                                                                                                                        >  2:78417/1‑130 (MQ=255)

              caATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAg                                                                                                                              <  1:1494160/140‑1 (MQ=255)

                ctGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGGCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGtt                                                                                                                            >  2:1253648/2‑140 (MQ=255)

                aTGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGtt                                                                                                                            >  2:817225/1‑140 (MQ=255)

                  gTCAAAGCCATGGTGAACTGAATAGCCCCTTTGCTTAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGATTCATTCCTTGATCCCTACGTATTTAAAGTTGt                                                                                                                          <  2:575272/140‑1 (MQ=255)

                  gTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGt                                                                                                                          <  2:2307254/140‑1 (MQ=255)

                       aGCCATGGGGAACTGAATTGCCCGTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                     <  2:1403999/140‑1 (MQ=255)

                       aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                     <  2:1571278/140‑1 (MQ=255)

                       aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                     <  2:1485318/140‑1 (MQ=255)

                       aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                     <  2:79805/140‑1 (MQ=255)

                       aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGGCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                     <  2:1946718/140‑1 (MQ=255)

                           aTGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTGTTTGATAGCGGATGTCGTCTAGGCTGCATTCCGTGATCCCTACGTCTGTAAAGTTGTCTGAATACa                                                                                                                 >  1:1269923/1‑140 (MQ=255)

                            tGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACAt                                                                                                                <  2:656049/140‑1 (MQ=255)

                                   cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                         >  2:1682673/1‑140 (MQ=255)

                                   cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                         >  2:2409496/1‑140 (MQ=255)

                                   cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                         >  1:1658640/1‑140 (MQ=255)

                                   cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                         >  2:594/1‑140 (MQ=255)

                                   cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                         >  2:759292/1‑140 (MQ=255)

                                   cTGAATAGCCCCTTTGCGGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                         >  2:1137519/1‑140 (MQ=255)

                                      aaTAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAAcc                                                                                                      >  1:665187/1‑140 (MQ=255)

                                           ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                 >  2:2216631/1‑140 (MQ=255)

                                                ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttg                                                                                            >  1:2101251/1‑139 (MQ=255)

                                                ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttg                                                                                            >  1:1718628/1‑139 (MQ=255)

                                                ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttc                                                                                            >  2:2144164/1‑140 (MQ=255)

                                                ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCGTTAACGTTGTCTGAATACATCCGGACAACCCCGTCGtttt                                                                                            >  2:1280363/1‑139 (MQ=255)

                                                ctgttGAAATTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGtttc                                                                                            >  1:2186953/5‑140 (MQ=255)

                                                ctgttGAAATTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGtttc                                                                                            >  1:777054/5‑140 (MQ=255)

                                                    tGAAATTCATAAATGACGCCATTCGGATAGGACGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCAGTCCGTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttctttc                                                                                        <  2:1249214/140‑1 (MQ=255)

                                                      aaaTTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTCAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTg                                                                                      >  2:2250059/1‑140 (MQ=255)

                                                      aaaTTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTg                                                                                      >  2:511526/1‑140 (MQ=255)

                                                        aTTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCa                                                                                    >  2:1519008/1‑140 (MQ=255)

                                                          tCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAc                                                                                  <  2:25291/140‑1 (MQ=255)

                                                             tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                               <  1:1412100/140‑1 (MQ=255)

                                                               aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                             <  1:74920/140‑1 (MQ=255)

                                                               aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                             <  1:1600571/140‑1 (MQ=255)

                                                               aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                             <  1:1923002/140‑1 (MQ=255)

                                                               aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                             <  1:1736011/140‑1 (MQ=255)

                                                                   aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTCCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                         <  1:1858730/140‑1 (MQ=255)

                                                                   aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                         <  2:132293/140‑1 (MQ=255)

                                                                   aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                         <  2:235767/140‑1 (MQ=255)

                                                                     gCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATg                                                                       <  2:2243866/140‑1 (MQ=255)

                                                                      ccTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTCTGCAACAGCAGCCTATGt                                                                      >  1:1036664/1‑140 (MQ=255)

                                                                       cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                     <  1:81083/140‑1 (MQ=255)

                                                                       cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                     <  1:887687/140‑1 (MQ=255)

                                                                       cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                     <  1:1161234/140‑1 (MQ=255)

                                                                       cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                     <  2:1156542/140‑1 (MQ=255)

                                                                       cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                     <  1:33044/140‑1 (MQ=255)

                                                                                  ttCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATAAATCCGGACAACCCCGTCGTGTCTTTGTGCAAAAGCAGCATAAGTAACCATATTCAg                                                          >  2:1748247/1‑140 (MQ=255)

                                                                                  ttCGCCCAGGCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAAGTCTTTCAAGTTGTCTGAATACATCCGGTCAACCGCGTCGTTTAGTTCTTGAACAACCGCAAATGTACCCAGTGTGAg                                                          >  1:1955363/1‑140 (MQ=255)

                                                                                    cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                        <  2:217672/140‑1 (MQ=255)

                                                                                    cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                        <  2:1369424/140‑1 (MQ=255)

                                                                                    cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                        <  2:2084450/140‑1 (MQ=255)

                                                                                    cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                        <  2:1980168/140‑1 (MQ=255)

                                                                                      cccAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcgcg                                                      <  2:1718628/140‑1 (MQ=255)

                                                                                        cAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCa                                                    <  1:296496/140‑1 (MQ=255)

                                                                                            cgtcTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGa                                                <  2:1581421/140‑1 (MQ=255)

                                                                                                      ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                      <  1:139811/140‑1 (MQ=255)

                                                                                                      ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                      <  2:1342306/140‑1 (MQ=255)

                                                                                                        gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                    <  1:2147073/140‑1 (MQ=255)

                                                                                                        gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                    <  2:845846/140‑1 (MQ=255)

                                                                                                        gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                    <  1:1519008/140‑1 (MQ=255)

                                                                                                            gCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACAGCAGCATATGTACCCAATTTCAGCGCGCACGGAAACACAAGCccgcccg                                >  1:1223825/1‑138 (MQ=255)

                                                                                                              ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCTCACGGAAACACATGCCCGCCGTta                              <  2:388619/140‑1 (MQ=255)

                                                                                                              ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTta                              <  1:1312461/140‑1 (MQ=255)

                                                                                                              ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTta                              <  2:2101251/140‑1 (MQ=255)

                                                                                                               gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                             <  1:2391983/140‑1 (MQ=255)

                                                                                                               gATGTCGTCTAGGCTGCAGTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                             <  2:855421/140‑1 (MQ=255)

                                                                                                                 tGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATaa                           <  1:1257200/140‑1 (MQ=255)

                                                                                                                 tGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATaa                           <  1:594/140‑1 (MQ=255)

                                                                                                                     gtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttctttc                                                                                        <  1:169331/75‑1 (MQ=255)

                                                                                                                     gtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttctttc                                                                                        >  2:169331/1‑75 (MQ=255)

                                                                                                                        aaGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAAGACATGCCCGCCGTTATAGTCTGTAt                    <  2:719533/139‑1 (MQ=255)

                                                                                                                                  tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt          <  2:1948475/140‑1 (MQ=255)

                                                                                                                                  tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt          <  2:1915024/140‑1 (MQ=255)

                                                                                                                                  tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt          <  1:2217883/140‑1 (MQ=255)

                                                                                                                                  tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt          <  1:1172261/140‑1 (MQ=255)

                                                                                                                                      tGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAACAGCAGCATATGTACCCATTTTGAGCGCGCACGGTAAAACAAGCCCGCCGTTATAATATGTATTTTGTGCAATCAGa      >  2:356357/1‑140 (MQ=255)

                                                                                                                                      tGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACACCAGCAAATGTACCCCTTTTCAGCGCGCACGGAAACAAATGCCCCCCGTTATAAACTGTAAGTTGTACAAACAGa      >  2:356098/1‑140 (MQ=255)

                                                                                                                                       gATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTATTGAAAAGCACCAAATGTAACCATTGTGACCGCGCACCGGAACAAAAGCCCCCCCGTATAAAATGTATGTTGTCCAAAAAGAt     >  1:232135/1‑140 (MQ=255)

                                                                                                                                         tCCCTACGTCTTTAAAGGTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCCTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc   <  1:1247282/140‑1 (MQ=255)

ATTCCCTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCA  >  NZ_CP034943/3746708‑3746985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`