BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,746,850	A→G	5.8%	V73A (GTA→GCA)	EO946_RS19275 ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,746,850	0	A	G	5.8%	607.4 / 18.2	191	V73A (GTA→GCA)	EO946_RS19275	galactokinase
Reads supporting (aligned to +/- strand): ref base A (64/114); new base G (7/4); total (72/119)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.09e-01

Only 100 of 203 total aligned reads displayed.

CCCTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCT  >  NZ_CP034943/3746711‑3746989

cccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTa                                                                                                                                             <  1:1102439/140‑1 (MQ=255)

cccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTa                                                                                                                                             >  1:962107/1‑140 (MQ=255)

cccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTa                                                                                                                                             <  1:729229/140‑1 (MQ=255)

cccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTa                                                                                                                                             <  1:645918/140‑1 (MQ=255)

cccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTa                                                                                                                                             >  1:57858/1‑140 (MQ=255)

cccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTa                                                                                                                                             <  1:255508/140‑1 (MQ=255)

 ccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAc                                                                                                                                            <  1:700510/140‑1 (MQ=255)

 ccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAc                                                                                                                                            <  1:675412/140‑1 (MQ=255)

 ccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAc                                                                                                                                            <  1:52117/140‑1 (MQ=255)

 ccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAc                                                                                                                                            <  1:454076/140‑1 (MQ=255)

 ccTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAc                                                                                                                                            <  1:308848/140‑1 (MQ=255)

   tGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCCTTCCTTGATCCCTACGt                                                                                                                                          >  2:2000421/1‑140 (MQ=255)

    gAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTATGCTGCATTCCTTGATCCCTACGTc                                                                                                                                         >  1:1674954/1‑140 (MQ=255)

    gAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTc                                                                                                                                         >  1:2416497/1‑140 (MQ=255)

          acaATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTaaa                                                                                                                                   >  1:1732501/1‑140 (MQ=255)

           caATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAc                                                                                                                                            >  2:78417/1‑130 (MQ=255)

             aTGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGtt                                                                                                                                >  2:817225/1‑140 (MQ=255)

               gTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGt                                                                                                                              <  1:1711454/140‑1 (MQ=255)

               gTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGt                                                                                                                              <  2:17028/140‑1 (MQ=255)

                    aGCCATGGGGAACTGAATTGCCCGTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                         <  2:1403999/140‑1 (MQ=255)

                    aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATACATGACGCCTATCGGATAGTTCGCCCAGTCGTCGTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTATAAAGTTGTCTGaa                                                                                                                         <  2:2240/140‑1 (MQ=255)

                    aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                         <  2:1485318/140‑1 (MQ=255)

                    aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                         <  2:518205/140‑1 (MQ=255)

                    aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                         <  2:79805/140‑1 (MQ=255)

                    aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                         <  2:1571278/140‑1 (MQ=255)

                    aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGGCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                         <  2:1946718/140‑1 (MQ=255)

                    aGCCAGGGGGAACTGAATAGCCCCTTTGCTGAAATACATCACTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                         <  2:1669280/140‑1 (MQ=255)

                        aTGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTGTTTGATAGCGGATGTCGTCTAGGCTGCATTCCGTGATCCCTACGTCTGTAAAGTTGTCTGAATACa                                                                                                                     >  1:1269923/1‑140 (MQ=255)

                         tGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACAt                                                                                                                    <  2:404417/140‑1 (MQ=255)

                         tGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACAt                                                                                                                    <  2:656049/140‑1 (MQ=255)

                          ggggAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATc                                                                                                                   >  1:2121627/1‑140 (MQ=255)

                              aaCTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGa                                                                                                               <  1:2190658/140‑1 (MQ=255)

                                cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCCGTCGTCTTCTTTTTGATAGCGGATGTCGTCGAGGCTGCATTCCTGGTTCCCTCCGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                             >  2:1257200/1‑140 (MQ=255)

                                cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                             >  2:759292/1‑140 (MQ=255)

                                cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                             >  1:44381/1‑140 (MQ=255)

                                   aaTAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAAcc                                                                                                          >  1:665187/1‑140 (MQ=255)

                                        ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                     >  2:2216631/1‑140 (MQ=255)

                                        ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                     >  2:1172261/1‑140 (MQ=255)

                                             ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttc                                                                                                >  2:2144164/1‑140 (MQ=255)

                                             ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttc                                                                                                >  2:2407776/1‑140 (MQ=255)

                                             ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttc                                                                                                >  1:1572623/1‑140 (MQ=255)

                                             ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGttta                                                                                                >  2:2245245/1‑139 (MQ=255)

                                             ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGATGCATTCATTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttg                                                                                                >  2:1365965/1‑139 (MQ=255)

                                             ctgttGAAATTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGtttc                                                                                                >  1:777054/5‑140 (MQ=255)

                                                   aaaTTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTATTTATg                                                                                          >  2:1340800/1‑140 (MQ=255)

                                                    aaTTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCCTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGc                                                                                         <  2:588776/140‑1 (MQ=255)

                                                     aTTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCa                                                                                        >  2:1519008/1‑140 (MQ=255)

                                                          tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                   <  1:1412100/140‑1 (MQ=255)

                                                           aaaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagca                                                                                  <  2:1378122/140‑1 (MQ=255)

                                                            aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                 <  1:74920/140‑1 (MQ=255)

                                                            aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                 <  1:1437432/140‑1 (MQ=255)

                                                            aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                 <  1:2248263/140‑1 (MQ=255)

                                                            aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                 <  1:1923002/140‑1 (MQ=255)

                                                              tGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAgcagca                                                                               <  1:2358654/140‑1 (MQ=255)

                                                                aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTCCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                             <  1:1858730/140‑1 (MQ=255)

                                                                   ccTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCGTTCTGCAAAAGCAGCaaattt                                                                          >  2:371951/1‑138 (MQ=255)

                                                                   ccTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTCTGCAACAGCAGCCTATGt                                                                          >  1:1036664/1‑140 (MQ=255)

                                                                    cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                         <  2:1156431/140‑1 (MQ=255)

                                                                    cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                         <  1:1161234/140‑1 (MQ=255)

                                                                    cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                         <  2:245063/140‑1 (MQ=255)

                                                                    cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTGTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGGTGTCTGAATACATCCGGACAACCCCGTCGTTTCGTTCTGCAACAGCAGCATATGTa                                                                         <  1:1319697/140‑1 (MQ=255)

                                                                               ttCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATAAATCCGGACAACCCCGTCGTGTCTTTGTGCAAAAGCAGCATAAGTAACCATATTCAg                                                              >  2:1748247/1‑140 (MQ=255)

                                                                               ttCGCCCAGGCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAAGTCTTTCAAGTTGTCTGAATACATCCGGTCAACCGCGTCGTTTAGTTCTTGAACAACCGCAAATGTACCCAGTGTGAg                                                              >  1:1955363/1‑140 (MQ=255)

                                                                                 cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGTCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                            <  2:259971/140‑1 (MQ=255)

                                                                                 cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                            <  2:1980168/140‑1 (MQ=255)

                                                                                 cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                            <  2:2084450/140‑1 (MQ=255)

                                                                                 cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                            <  2:217672/140‑1 (MQ=255)

                                                                                   cccAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcgcg                                                          <  1:2175893/140‑1 (MQ=255)

                                                                                     cAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCa                                                        <  1:20400/140‑1 (MQ=255)

                                                                                     cAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCa                                                        <  1:80913/140‑1 (MQ=255)

                                                                                         cgtcTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGa                                                    <  2:1581421/140‑1 (MQ=255)

                                                                                         cgtcTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGa                                                    <  2:1250537/140‑1 (MQ=255)

                                                                                                  tttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTCTGCAACAGCAGCATATGTACCCATTTTCAGCGCGCACGGAAACACATGc                                           >  2:2349221/1‑140 (MQ=255)

                                                                                                   ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                          <  1:139811/140‑1 (MQ=255)

                                                                                                   ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCCGCAACAGCAGCATATGGACCCATTGTCAGCGCGCACGGAAACACATGcc                                          <  1:1616418/140‑1 (MQ=255)

                                                                                                     gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                        <  2:845846/140‑1 (MQ=255)

                                                                                                     gATAGCGGATGTCGTCTAGGCTGCAGTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                        <  1:848084/140‑1 (MQ=255)

                                                                                                         gCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACAGCAGCATATGTACCCAATTTCAGCGCGCACGGAAACACAAGCccgcccg                                    >  1:1223825/1‑138 (MQ=255)

                                                                                                          cGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACACCAGCAAATGTACCCAATGTAAGCGCGCACCGAAACACAAGCcccccggt                                   >  2:1312461/1‑138 (MQ=255)

                                                                                                          cGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTGTGCAACAGCACCATATGTACCCATTGTCAGCGCGCACGGAAAAAAAATCCCCCCGtt                                   >  1:1791359/1‑140 (MQ=255)

                                                                                                           ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTta                                  <  2:2101251/140‑1 (MQ=255)

                                                                                                           ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTta                                  <  1:1503463/140‑1 (MQ=255)

                                                                                                            gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                 <  2:500269/140‑1 (MQ=255)

                                                                                                            gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                 <  1:2391983/140‑1 (MQ=255)

                                                                                                            gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                 <  1:1682673/140‑1 (MQ=255)

                                                                                                            gATGTCGTCTAGGCTGCAGTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                 <  2:855421/140‑1 (MQ=255)

                                                                                                              tGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATaa                               <  1:594/140‑1 (MQ=255)

                                                                                                              tGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATaa                               <  1:1257200/140‑1 (MQ=255)

                                                                                                                    cTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATAAGTACCCATTGTCAACGCGCACCGAAACACATGCCCGCCGGTAAAATCTGTa                         >  2:2269744/1‑140 (MQ=255)

                                                                                                                    cTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAACAGCAGCAAATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCCCCGGTATAATCTgga                         >  2:1773628/1‑138 (MQ=255)

                                                                                                                     aaGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAAGACATGCCCGCCGTTATAGTCTGTAt                        <  2:719533/139‑1 (MQ=255)

                                                                                                                               tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt              <  2:1948475/140‑1 (MQ=255)

                                                                                                                               tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt              <  2:1915024/140‑1 (MQ=255)

                                                                                                                               tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt              <  1:2216631/140‑1 (MQ=255)

                                                                                                                               tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt              <  1:1172261/140‑1 (MQ=255)

                                                                                                                                   tGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGGATATGTACCCATTGTCAGCGCGCAAGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGa          <  2:1693290/140‑1 (MQ=255)

                                                                                                                                   tGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTCTGCAACAGCAGCATATGTACCCCTTGTCAGCGCGCACGGGAACACATGCCCGCCGTTATAATCAGTATGTTGTCCAATCAGa          >  2:356142/1‑140 (MQ=255)

                                                                                                                                      tCCCTACGTCTTTAAAGGTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCCTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc       <  1:1247282/140‑1 (MQ=255)

                                                                                                                                       cccTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCAATGTCAGCGCGCACGGACACACCTGCCCGCCCTTATAAAAAGTATGTTCTCCCATCAGATTCa      >  2:1035081/1‑140 (MQ=255)

                                                                                                                                           aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTGTGCAACACCAGCAAATTTACCCATTGTCAGCGCGCCCCGAAACACATGCCCCCCCGTATAAACTGTATTTTTTGCAATAAGATTAAcact  >  1:2075220/1‑140 (MQ=255)

CCCTGAAAACACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCT  >  NZ_CP034943/3746711‑3746989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`