BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,746,856	T→C	6.3%	K71R (AAA→AGA)	EO946_RS19275 ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,746,856	0	T	C	6.3%	559.1 / 17.7	177	K71R (AAA→AGA)	EO946_RS19275	galactokinase
Reads supporting (aligned to +/- strand): ref base T (58/106); new base C (7/4); total (66/111)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

ACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCT  >  NZ_CP034943/3746721‑3746989

acaATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTaaa                                                                                                                                   >  1:1732501/1‑140 (MQ=255)

 caATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAg                                                                                                                                  <  1:1494160/140‑1 (MQ=255)

   ctGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGGCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGtt                                                                                                                                >  2:1253648/2‑140 (MQ=255)

   aTGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGtt                                                                                                                                >  2:817225/1‑140 (MQ=255)

   aTGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGtt                                                                                                                                >  2:1963799/1‑140 (MQ=255)

     gTCAAAGCCATGGTGAACTGAATAGCCCCTTTGCTTAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGATTCATTCCTTGATCCCTACGTATTTAAAGTTGt                                                                                                                              <  2:575272/140‑1 (MQ=255)

     gTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGt                                                                                                                              <  2:2307254/140‑1 (MQ=255)

     gTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGt                                                                                                                              <  2:453981/140‑1 (MQ=255)

     gTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGt                                                                                                                              <  2:17028/140‑1 (MQ=255)

     gTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGt                                                                                                                              <  1:1711454/140‑1 (MQ=255)

          aGCCATGGGGAACTGAATTGCCCGTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                         <  2:1403999/140‑1 (MQ=255)

          aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATACATGACGCCTATCGGATAGTTCGCCCAGTCGTCGTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTATAAAGTTGTCTGaa                                                                                                                         <  2:2240/140‑1 (MQ=255)

          aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                         <  2:1485318/140‑1 (MQ=255)

          aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                         <  2:79805/140‑1 (MQ=255)

          aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGGCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                         <  2:1946718/140‑1 (MQ=255)

          aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCATTCGGATAGTTCGCCCAGTCGTATTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                         <  2:79256/140‑1 (MQ=255)

          aGCCAGGGGGAACTGAATAGCCCCTTTGCTGAAATACATCACTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                         <  2:1669280/140‑1 (MQ=255)

              aTGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTGTTTGATAGCGGATGTCGTCTAGGCTGCATTCCGTGATCCCTACGTCTGTAAAGTTGTCTGAATACa                                                                                                                     >  1:1269923/1‑140 (MQ=255)

               tGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACAt                                                                                                                    <  2:656049/140‑1 (MQ=255)

                ggggAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATc                                                                                                                   >  1:2121627/1‑140 (MQ=255)

                ggggAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCGTTTTGATAGCGGATGTCGTATAGGGTGCATTCCATGATCCCTACGTCTTTAAAGTTGTCTGAATACaac                                                                                                                   >  1:677999/1‑138 (MQ=255)

                      cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                             >  2:759292/1‑140 (MQ=255)

                      cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                             >  1:44381/1‑140 (MQ=255)

                      cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                             >  2:594/1‑140 (MQ=255)

                      cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                             >  2:2409496/1‑140 (MQ=255)

                         aaTAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAAcc                                                                                                          >  1:665187/1‑140 (MQ=255)

                              ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                     >  1:1942794/1‑140 (MQ=255)

                              ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                     >  2:1172261/1‑140 (MQ=255)

                              ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                     >  2:2216631/1‑140 (MQ=255)

                                   ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttc                                                                                                >  2:2144164/1‑140 (MQ=255)

                                   ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGttta                                                                                                >  2:2245245/1‑139 (MQ=255)

                                   ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCGTTAACGTTGTCTGAATACATCCGGACAACCCCGTCGtttt                                                                                                >  2:1280363/1‑139 (MQ=255)

                                   ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGATGCATTCATTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttg                                                                                                >  2:1365965/1‑139 (MQ=255)

                                   ctgttGAAATTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGtttc                                                                                                >  1:719533/5‑140 (MQ=255)

                                   ctgttGAAATTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGtttc                                                                                                >  1:777054/5‑140 (MQ=255)

                                   ctgttGAAATTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGtttc                                                                                                >  1:2186953/5‑140 (MQ=255)

                                       tGAAATTCATAAATGACGCCATTCGGATAGGACGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCAGTCCGTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttctttc                                                                                            <  2:1249214/140‑1 (MQ=255)

                                         aaaTTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTCAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTg                                                                                          >  2:2250059/1‑140 (MQ=255)

                                         aaaTTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTg                                                                                          >  2:511526/1‑140 (MQ=255)

                                         aaaTTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATACGGACAAGCCCGTCGTTTCTTTATg                                                                                          >  2:160266/1‑140 (MQ=255)

                                            ttCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCaa                                                                                       <  2:1737525/140‑1 (MQ=255)

                                                ttactGACGCCTTTCGGAGAGGTCGCCCAGTCGTGTTCTGTGTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGACTACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                   <  2:1756670/136‑1 (MQ=255)

                                                tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                   <  2:2129525/140‑1 (MQ=255)

                                                 aaaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagca                                                                                  <  2:1378122/140‑1 (MQ=255)

                                                 aaaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagca                                                                                  <  1:2399176/140‑1 (MQ=255)

                                                  aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                 <  1:1600571/140‑1 (MQ=255)

                                                  aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                 <  1:1736011/140‑1 (MQ=255)

                                                  aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                 <  1:2248263/140‑1 (MQ=255)

                                                  aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                 <  1:74920/140‑1 (MQ=255)

                                                    tGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAgcagca                                                                               <  1:2358654/140‑1 (MQ=255)

                                                      aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                             <  2:235767/140‑1 (MQ=255)

                                                      aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                             <  2:132293/140‑1 (MQ=255)

                                                        ggcTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGAGGTCGTCTAGGCTGCATTCCTTGATCCCTACGTGTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATg                                                                           <  2:1516176/138‑1 (MQ=255)

                                                         ccTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCGTTCTGCAAAAGCAGCaaattt                                                                          >  2:371951/1‑138 (MQ=255)

                                                         ccTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTCTGCAACAGCAGCCTATGt                                                                          >  1:1036664/1‑140 (MQ=255)

                                                          cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                         <  2:245063/140‑1 (MQ=255)

                                                          cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                         <  1:1419151/140‑1 (MQ=255)

                                                          cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                         <  1:265647/140‑1 (MQ=255)

                                                          cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                         <  1:33044/140‑1 (MQ=255)

                                                          cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                         <  1:81083/140‑1 (MQ=255)

                                                          cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                         <  2:1156431/140‑1 (MQ=255)

                                                          cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTGTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGGTGTCTGAATACATCCGGACAACCCCGTCGTTTCGTTCTGCAACAGCAGCATATGTa                                                                         <  1:1319697/140‑1 (MQ=255)

                                                                    gTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTGCTTTCTGCAACAGCACCAAAAGTAACCAgtgtaa                                                               >  1:1868264/1‑138 (MQ=255)

                                                                     ttCGCCCAGGCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAAGTCTTTCAAGTTGTCTGAATACATCCGGTCAACCGCGTCGTTTAGTTCTTGAACAACCGCAAATGTACCCAGTGTGAg                                                              >  1:1955363/1‑140 (MQ=255)

                                                                       cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGTCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                            <  2:259971/140‑1 (MQ=255)

                                                                       cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                            <  2:2084450/140‑1 (MQ=255)

                                                                       cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                            <  2:217672/140‑1 (MQ=255)

                                                                         cccAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcgcg                                                          <  2:1572623/140‑1 (MQ=255)

                                                                         cccAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcgcg                                                          <  1:2175893/140‑1 (MQ=255)

                                                                           cAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCa                                                        <  1:1165079/140‑1 (MQ=255)

                                                                           cAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCa                                                        <  1:20400/140‑1 (MQ=255)

                                                                                        tttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTATGCAACAGCACCATAAGTAACCATTGTCAGCGCGCACCGCAAcacaatc                                           >  2:152295/1‑137 (MQ=255)

                                                                                         ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                          <  2:1342306/140‑1 (MQ=255)

                                                                                         ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                          <  1:139811/140‑1 (MQ=255)

                                                                                         ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                          <  1:139914/140‑1 (MQ=255)

                                                                                         ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCCGCAACAGCAGCATATGGACCCATTGTCAGCGCGCACGGAAACACATGcc                                          <  1:1616418/140‑1 (MQ=255)

                                                                                           gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                        <  1:1519008/140‑1 (MQ=255)

                                                                                           gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                        <  2:845846/140‑1 (MQ=255)

                                                                                           gATAGCGGATGTCGTCTAGGCTGCAGTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                        <  1:848084/140‑1 (MQ=255)

                                                                                               gCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACAGCAGCATATGTACCCAATTTCAGCGCGCACGGAAACACAAGCccgcccg                                    >  1:1223825/1‑138 (MQ=255)

                                                                                                 ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCTCACGGAAACACATGCCCGCCGTta                                  <  2:388619/140‑1 (MQ=255)

                                                                                                  gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                 <  1:1037616/140‑1 (MQ=255)

                                                                                                  gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                 <  2:1985551/140‑1 (MQ=255)

                                                                                                  gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                 <  1:1682673/140‑1 (MQ=255)

                                                                                                  gATGTCGTCTAGGCTGCAGTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                 <  2:855421/140‑1 (MQ=255)

                                                                                                  gATGTAGTATAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                 <  1:548533/140‑1 (MQ=255)

                                                                                                    tGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATaa                               <  1:1257200/140‑1 (MQ=255)

                                                                                                    tGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATaa                               <  1:594/140‑1 (MQ=255)

                                                                                                          cTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAACAGCAGCAAATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCCCCGGTATAATCTgga                         >  2:1773628/1‑138 (MQ=255)

                                                                                                           aaGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAAGACATGCCCGCCGTTATAGTCTGTAt                        <  2:719533/139‑1 (MQ=255)

                                                                                                                     tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt              <  2:1948475/140‑1 (MQ=255)

                                                                                                                     tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt              <  1:2216631/140‑1 (MQ=255)

                                                                                                                     tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt              <  2:1915024/140‑1 (MQ=255)

                                                                                                                         tGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGGATATGTACCCATTGTCAGCGCGCAAGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGa          <  2:1693290/140‑1 (MQ=255)

                                                                                                                         tGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTCTGCAACAGCAGCATATGTACCCCTTGTCAGCGCGCACGGGAACACATGCCCGCCGTTATAATCAGTATGTTGTCCAATCAGa          >  2:356142/1‑140 (MQ=255)

                                                                                                                            tCCCTACGTCTTTAAAGGTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCCTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc       <  1:1247282/140‑1 (MQ=255)

                                                                                                                             cccTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCAATGTCAGCGCGCACGGACACACCTGCCCGCCCTTATAAAAAGTATGTTCTCCCATCAGATTCa      >  2:1035081/1‑140 (MQ=255)

                                                                                                                                tACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAACAGCAGCCTATGTACCCATTGTCAGCGCGCCCCGGAACACAAGCCCGCCGGTCTTATCTTTATTTTGTCCCATCAGATTAAcgc   >  1:2373691/1‑138 (MQ=255)

                                                                                                                                tACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTGTGCAACACCAGCATATGTACCCATTGTCAGCGCCCACGGAAACACATGCCCGCCCGTATAAAATTTATTTTCTCCAATCCGAttaaata   >  1:1822109/1‑135 (MQ=255)

                                                                                                                                 aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTGTGCAACACCAGCAAATTTACCCATTGTCAGCGCGCCCCGAAACACATGCCCCCCCGTATAAACTGTATTTTTTGCAATAAGATTAAcact  >  1:2075220/1‑140 (MQ=255)

ACAATGTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCT  >  NZ_CP034943/3746721‑3746989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`