Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,746,864 G→A 6.4% D68D (GAC→GAT EO946_RS19275 ← galactokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,746,8640GA 6.4% 556.0 / 18.0 172D68D (GAC→GATEO946_RS19275galactokinase
Reads supporting (aligned to +/- strand):  ref base G (56/105);  new base A (7/4);  total (63/109)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 178 total aligned reads displayed.

GTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAA  >  NZ_CP034943/3746726‑3747003
                                                                                                                                          |                                                                                                                                           
gTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGt                                                                                                                                            <  2:2307254/140‑1 (MQ=255)
gTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGt                                                                                                                                            <  2:17028/140‑1 (MQ=255)
     aGCCATGGGGAACTGAATTGCCCGTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                                       <  2:1403999/140‑1 (MQ=255)
     aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATACATGACGCCTATCGGATAGTTCGCCCAGTCGTCGTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTATAAAGTTGTCTGaa                                                                                                                                       <  2:2240/140‑1 (MQ=255)
     aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                                       <  2:518205/140‑1 (MQ=255)
     aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                                       <  2:1946719/140‑1 (MQ=255)
     aGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCATTCGGATAGTTCGCCCAGTCGTATTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                                       <  2:79256/140‑1 (MQ=255)
     aGCCAGGGGGAACTGAATAGCCCCTTTGCTGAAATACATCACTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGaa                                                                                                                                       <  2:1669280/140‑1 (MQ=255)
          tGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACAt                                                                                                                                  <  2:404417/140‑1 (MQ=255)
           ggggAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATc                                                                                                                                 >  1:2121627/1‑140 (MQ=255)
           ggggAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCGTTTTGATAGCGGATGTCGTATAGGGTGCATTCCATGATCCCTACGTCTTTAAAGTTGTCTGAATACaac                                                                                                                                 >  1:677999/1‑138 (MQ=255)
               aaCTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGa                                                                                                                             <  1:2190658/140‑1 (MQ=255)
                 cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAATGTTGTCTGAATTCATCCggtcg                                                                                                                           >  2:1453531/1‑137 (MQ=255)
                 cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                                           >  2:2409496/1‑140 (MQ=255)
                 cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                                           >  1:1658640/1‑140 (MQ=255)
                 cTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                                           >  2:594/1‑140 (MQ=255)
                 cTGAATAGCCCCTTTGCGGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACa                                                                                                                           >  2:1137519/1‑140 (MQ=255)
                    aaTAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAAcc                                                                                                                        >  1:665187/1‑140 (MQ=255)
                         ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                                   >  2:2216631/1‑140 (MQ=255)
                         ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                                   >  2:1172261/1‑140 (MQ=255)
                         ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                                   >  1:1942794/1‑140 (MQ=255)
                         ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCGTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                                   >  1:453064/1‑140 (MQ=255)
                         ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCCTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                                   >  1:588776/1‑140 (MQ=255)
                              ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttg                                                                                                              >  1:1718628/1‑139 (MQ=255)
                              ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttc                                                                                                              >  1:1572623/1‑140 (MQ=255)
                              ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttc                                                                                                              >  2:2144164/1‑140 (MQ=255)
                              ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGttta                                                                                                              >  2:2245245/1‑139 (MQ=255)
                              ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCGTTAACGTTGTCTGAATACATCCGGACAACCCCGTCGtttt                                                                                                              >  2:1280363/1‑139 (MQ=255)
                              ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGATGCATTCATTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttg                                                                                                              >  2:1365965/1‑139 (MQ=255)
                              ctgttGAAATTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGtttc                                                                                                              >  1:777054/5‑140 (MQ=255)
                                    aaaTTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTATTTATg                                                                                                        >  2:1340800/1‑140 (MQ=255)
                                    aaaTTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTCAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTg                                                                                                        >  2:2250059/1‑140 (MQ=255)
                                    aaaTTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATACGGACAAGCCCGTCGTTTCTTTATg                                                                                                        >  2:160266/1‑140 (MQ=255)
                                     aaTTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGc                                                                                                       <  2:1942794/140‑1 (MQ=255)
                                     aaTTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCCTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGc                                                                                                       <  2:588776/140‑1 (MQ=255)
                                       ttCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCaa                                                                                                     <  2:1737525/140‑1 (MQ=255)
                                        tCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAc                                                                                                    <  2:25291/140‑1 (MQ=255)
                                           tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                 <  1:1412100/140‑1 (MQ=255)
                                           tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                 <  2:2129525/140‑1 (MQ=255)
                                            aaaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagca                                                                                                <  1:1258345/140‑1 (MQ=255)
                                            aaaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagca                                                                                                <  1:2399176/140‑1 (MQ=255)
                                            aaaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagca                                                                                                <  2:1378122/140‑1 (MQ=255)
                                             aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                               <  1:2248263/140‑1 (MQ=255)
                                             aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                               <  1:74920/140‑1 (MQ=255)
                                               tGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAgcagca                                                                                             <  1:2358654/140‑1 (MQ=255)
                                               tGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAgcagca                                                                                             <  1:2430724/140‑1 (MQ=255)
                                                 aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTCCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                                           <  1:1858730/140‑1 (MQ=255)
                                                 aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                                           <  2:235767/140‑1 (MQ=255)
                                                 aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                                           <  2:132293/140‑1 (MQ=255)
                                                 aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                                           <  1:2012062/140‑1 (MQ=255)
                                                 aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                                           <  1:73254/140‑1 (MQ=255)
                                                   ggcTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGAGGTCGTCTAGGCTGCATTCCTTGATCCCTACGTGTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATg                                                                                         <  2:1516176/138‑1 (MQ=255)
                                                    ccTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTCTGCAACAGCAGCCTATGt                                                                                        >  1:1036664/1‑140 (MQ=255)
                                                     cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                       <  1:265647/140‑1 (MQ=255)
                                                     cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                       <  1:1161234/140‑1 (MQ=255)
                                                     cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                       <  1:1419151/140‑1 (MQ=255)
                                                     cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                       <  1:81083/140‑1 (MQ=255)
                                                     cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                       <  1:887687/140‑1 (MQ=255)
                                                     cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                       <  2:1156431/140‑1 (MQ=255)
                                                     cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                       <  1:33044/140‑1 (MQ=255)
                                                               gTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTGCTTTCTGCAACAGCACCAAAAGTAACCAgtgtaa                                                                             >  1:1868264/1‑138 (MQ=255)
                                                                ttCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATAAATCCGGACAACCCCGTCGTGTCTTTGTGCAAAAGCAGCATAAGTAACCATATTCAg                                                                            >  2:1748247/1‑140 (MQ=255)
                                                                  cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                                          <  2:2084450/140‑1 (MQ=255)
                                                                  cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                                          <  2:217672/140‑1 (MQ=255)
                                                                    cccAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcgcg                                                                        <  2:1572623/140‑1 (MQ=255)
                                                                    cccAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcgcg                                                                        <  1:2175893/140‑1 (MQ=255)
                                                                          cgtcTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGa                                                                  <  2:1250537/140‑1 (MQ=255)
                                                                                   tttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTATGCAACAGCACCATAAGTAACCATTGTCAGCGCGCACCGCAAcacaatc                                                         >  2:152295/1‑137 (MQ=255)
                                                                                    ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                        <  1:139811/140‑1 (MQ=255)
                                                                                    ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCCGCAACAGCAGCATATGGACCCATTGTCAGCGCGCACGGAAACACATGcc                                                        <  1:1616418/140‑1 (MQ=255)
                                                                                      gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                      <  2:845846/140‑1 (MQ=255)
                                                                                          gCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCCACACCAGCATATTTACCCATTGTCAGCGCGCACCGAAACACATGccccccca                                                  >  1:1235475/1‑138 (MQ=255)
                                                                                           cGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTGTGCAACAGCACCATATGTACCCATTGTCAGCGCGCACGGAAAAAAAATCCCCCCGtt                                                 >  1:1791359/1‑140 (MQ=255)
                                                                                            ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCTCACGGAAACACATGCCCGCCGTta                                                <  2:388619/140‑1 (MQ=255)
                                                                                            ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTta                                                <  1:1503463/140‑1 (MQ=255)
                                                                                            ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTta                                                <  1:1312461/140‑1 (MQ=255)
                                                                                            ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTGAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATAGGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTta                                                <  2:813219/140‑1 (MQ=255)
                                                                                             gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                               <  2:500269/140‑1 (MQ=255)
                                                                                             gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                               <  1:1037616/140‑1 (MQ=255)
                                                                                             gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                               <  2:1985551/140‑1 (MQ=255)
                                                                                             gATGTCGTCTAGGCTGCAGTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                               <  2:855421/140‑1 (MQ=255)
                                                                                               tGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATaa                                             <  1:594/140‑1 (MQ=255)
                                                                                                   gtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttctttc                                                                                                          <  1:169331/75‑1 (MQ=255)
                                                                                                     cTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAACAGCAGCAAATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCCCCGGTATAATCTgga                                       >  2:1773628/1‑138 (MQ=255)
                                                                                                      aaGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAAGACATGCCCGCCGTTATAGTCTGTAt                                      <  2:777054/139‑1 (MQ=255)
                                                                                                      aaGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAAGACATGCCCGCCGTTATAGTCTGTAt                                      <  2:756602/139‑1 (MQ=255)
                                                                                                        ggCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTCTGCAAAAGCAGCATATGTAACCATTGTCAGCCCGCACGGAAACACATTCCCGCCGGTATAAAatgtatgt                                    >  2:675707/1‑140 (MQ=255)
                                                                                                                tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                            <  1:2217883/140‑1 (MQ=255)
                                                                                                                tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                            <  1:1172261/140‑1 (MQ=255)
                                                                                                                tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                            <  2:1915024/140‑1 (MQ=255)
                                                                                                                tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                            <  2:1948475/140‑1 (MQ=255)
                                                                                                                    tGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAACAGCAGCATATGTACCCATTTTGAGCGCGCACGGTAAAACAAGCCCGCCGTTATAATATGTATTTTGTGCAATCAGa                        >  2:356357/1‑140 (MQ=255)
                                                                                                                    tGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGGATATGTACCCATTGTCAGCGCGCAAGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGa                        <  2:1693290/140‑1 (MQ=255)
                                                                                                                    tGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACACCAGCAAATGTACCCCTTTTCAGCGCGCACGGAAACAAATGCCCCCCGTTATAAACTGTAAGTTGTACAAACAGa                        >  2:356098/1‑140 (MQ=255)
                                                                                                                       tCCCTACGTCTTTAAAGGTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCCTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc                     <  1:1247282/140‑1 (MQ=255)
                                                                                                                         ccTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCAc                   <  1:2074736/140‑1 (MQ=255)
                                                                                                                           tACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAACAGCAGCCTATGTACCCATTGTCAGCGCGCCCCGGAACACAAGCCCGCCGGTCTTATCTTTATTTTGTCCCATCAGATTAAcgc                 >  1:2373691/1‑138 (MQ=255)
                                                                                                                            aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTGTGCAACACCAGCAAATTTACCCATTGTCAGCGCGCCCCGAAACACATGCCCCCCCGTATAAACTGTATTTTTTGCAATAAGATTAAcact                >  1:2075220/1‑140 (MQ=255)
                                                                                                                                          gTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGaaaa  <  2:665187/140‑1 (MQ=255)
                                                                                                                                          gTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACAGCAGCATATGTAACCAATGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCACATTAACTCTTCCACGTGCGaaaa  >  2:2432048/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GTCAAAGCCATGGGGAACTGAATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAA  >  NZ_CP034943/3746726‑3747003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: