BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,746,884	C→G	6.2%	V62L (GTT→CTT)	EO946_RS19275 ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,746,884	0	C	G	6.2%	568.3 / 15.4	173	V62L (GTT→CTT)	EO946_RS19275	galactokinase
Reads supporting (aligned to +/- strand): ref base C (62/99); new base G (7/5); total (69/104)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

AATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTT  >  NZ_CP034943/3746746‑3747023

     ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                                                       >  2:1172261/1‑140 (MQ=255)

     ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                                                       >  1:1942794/1‑140 (MQ=255)

     ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                                                       >  2:2217883/1‑140 (MQ=255)

     ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCGTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                                                       >  1:453064/1‑140 (MQ=255)

     ccccTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCCTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCcgtc                                                                                                                                       >  1:588776/1‑140 (MQ=255)

          ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttc                                                                                                                                  >  2:2144164/1‑140 (MQ=255)

          ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGttta                                                                                                                                  >  2:2245245/1‑139 (MQ=255)

          ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGATGCATTCATTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttg                                                                                                                                  >  2:1365965/1‑139 (MQ=255)

          ctgttGAAATTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGtttc                                                                                                                                  >  1:719533/5‑140 (MQ=255)

          ctgttGAAATTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGtttc                                                                                                                                  >  1:2186953/5‑140 (MQ=255)

          ctgttGAAATTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGtttc                                                                                                                                  >  1:756602/5‑140 (MQ=255)

                aaaTTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTATTTATg                                                                                                                            >  2:1340800/1‑140 (MQ=255)

                aaaTTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTCAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTg                                                                                                                            >  2:2250059/1‑140 (MQ=255)

                aaaTTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTg                                                                                                                            >  2:511526/1‑140 (MQ=255)

                aaaTTCATAAATCACGCCTTTTGGGTAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATACGGACAAGCCCGTCGTTTCTTTATg                                                                                                                            >  2:160266/1‑140 (MQ=255)

                   ttCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCaa                                                                                                                         <  2:1737525/140‑1 (MQ=255)

                       tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                                     <  1:1412100/140‑1 (MQ=255)

                       tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                                     <  2:2129525/140‑1 (MQ=255)

                       tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                                     <  2:2148425/140‑1 (MQ=255)

                        aaaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagca                                                                                                                    <  1:1258345/140‑1 (MQ=255)

                         aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                   <  1:1437432/140‑1 (MQ=255)

                         aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                   <  1:1600571/140‑1 (MQ=255)

                         aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                   <  1:1736011/140‑1 (MQ=255)

                         aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                   <  1:1923002/140‑1 (MQ=255)

                         aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                   <  1:2248263/140‑1 (MQ=255)

                           tGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAgcagca                                                                                                                 <  1:2358654/140‑1 (MQ=255)

                           tGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAgcagca                                                                                                                 <  1:2430724/140‑1 (MQ=255)

                             aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTCCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                                                               <  1:1858730/140‑1 (MQ=255)

                             aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                                                               <  1:2012062/140‑1 (MQ=255)

                             aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                                                               <  2:132293/140‑1 (MQ=255)

                             aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                                                               <  2:235767/140‑1 (MQ=255)

                             aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCata                                                                                                               <  1:73254/140‑1 (MQ=255)

                               ggcTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGAGGTCGTCTAGGCTGCATTCCTTGATCCCTACGTGTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATg                                                                                                             <  2:1516176/138‑1 (MQ=255)

                                ccTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCGTTCTGCAAAAGCAGCaaattt                                                                                                            >  2:371951/1‑138 (MQ=255)

                                ccTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTCTGCAACAGCAGCCTATGt                                                                                                            >  1:1036664/1‑140 (MQ=255)

                                 cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                                           <  1:1161234/140‑1 (MQ=255)

                                 cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                                           <  2:1156431/140‑1 (MQ=255)

                                 cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                                           <  1:33044/140‑1 (MQ=255)

                                 cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                                           <  1:265647/140‑1 (MQ=255)

                                 cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                                           <  2:245063/140‑1 (MQ=255)

                                 cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTGTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGGTGTCTGAATACATCCGGACAACCCCGTCGTTTCGTTCTGCAACAGCAGCATATGTa                                                                                                           <  1:1319697/140‑1 (MQ=255)

                                            ttCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATAAATCCGGACAACCCCGTCGTGTCTTTGTGCAAAAGCAGCATAAGTAACCATATTCAg                                                                                                >  2:1748247/1‑140 (MQ=255)

                                            ttCGCCCAGGCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTAAGTCTTTCAAGTTGTCTGAATACATCCGGTCAACCGCGTCGTTTAGTTCTTGAACAACCGCAAATGTACCCAGTGTGAg                                                                                                >  1:1955363/1‑140 (MQ=255)

                                              cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGTCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                                                              <  2:259971/140‑1 (MQ=255)

                                              cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                                                              <  2:1369424/140‑1 (MQ=255)

                                              cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                                                              <  2:2084450/140‑1 (MQ=255)

                                                cccAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcgcg                                                                                            <  2:1572623/140‑1 (MQ=255)

                                                  cAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCa                                                                                          <  1:80913/140‑1 (MQ=255)

                                                  cAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCa                                                                                          <  1:20400/140‑1 (MQ=255)

                                                      cgtcTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGa                                                                                      <  2:1250537/140‑1 (MQ=255)

                                                               tttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTATGCAACAGCACCATAAGTAACCATTGTCAGCGCGCACCGCAAcacaatc                                                                             >  2:152295/1‑137 (MQ=255)

                                                                ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                            <  1:139914/140‑1 (MQ=255)

                                                                ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCCGCAACAGCAGCATATGGACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                            <  1:1616418/140‑1 (MQ=255)

                                                                  gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                          <  2:845846/140‑1 (MQ=255)

                                                                  gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                          <  1:2147073/140‑1 (MQ=255)

                                                                  gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                          <  1:1519008/140‑1 (MQ=255)

                                                                  gATAGCGGATGTCGTCTAGGCTGCAGTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                          <  1:848084/140‑1 (MQ=255)

                                                                      gCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCCACACCAGCATATTTACCCATTGTCAGCGCGCACCGAAACACATGccccccca                                                                      >  1:1235475/1‑138 (MQ=255)

                                                                        ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTta                                                                    <  2:2101251/140‑1 (MQ=255)

                                                                        ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTGAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATAGGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTta                                                                    <  2:813219/140‑1 (MQ=255)

                                                                         gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                                                   <  1:1037616/140‑1 (MQ=255)

                                                                         gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                                                   <  1:2391983/140‑1 (MQ=255)

                                                                         gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                                                   <  2:1985551/140‑1 (MQ=255)

                                                                         gATGTCGTCTAGGCTGCAGTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                                                   <  2:855421/140‑1 (MQ=255)

                                                                               gtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttctttc                                                                                                                              >  2:169331/1‑75 (MQ=255)

                                                                               gtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttctttc                                                                                                                              <  1:169331/75‑1 (MQ=255)

                                                                                 cTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAACAGCAGCAAATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCCCCGGTATAATCTgga                                                           >  2:1773628/1‑138 (MQ=255)

                                                                                  aaGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAAGACATGCCCGCCGTTATAGTCTGTAt                                                          <  2:777054/139‑1 (MQ=255)

                                                                                  aaGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAAGACATGCCCGCCGTTATAGTCTGTAt                                                          <  2:2186953/139‑1 (MQ=255)

                                                                                    ggCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTCTGCAAAAGCAGCATATGTAACCATTGTCAGCCCGCACGGAAACACATTCCCGCCGGTATAAAatgtatgt                                                        >  2:675707/1‑140 (MQ=255)

                                                                                         cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAACCCCAGCAAATGGACCCAATGTGACCGCGCACCGAAACAAATGCCCCCCGTTATAAACTTTAttttgtgc                                                   >  1:657016/1‑136 (MQ=255)

                                                                                            tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                <  2:1948475/140‑1 (MQ=255)

                                                                                            tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                <  2:1914789/140‑1 (MQ=255)

                                                                                            tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                <  1:2216631/140‑1 (MQ=255)

                                                                                            tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                <  1:1172261/140‑1 (MQ=255)

                                                                                                tGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAACAGCAGCATATGTACCCATTTTGAGCGCGCACGGTAAAACAAGCCCGCCGTTATAATATGTATTTTGTGCAATCAGa                                            >  2:356357/1‑140 (MQ=255)

                                                                                                tGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACACCAGCAAATGTACCCCTTTTCAGCGCGCACGGAAACAAATGCCCCCCGTTATAAACTGTAAGTTGTACAAACAGa                                            >  2:356098/1‑140 (MQ=255)

                                                                                                   tCCCTACGTCTTTAAAGGTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCCTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc                                         <  1:1247282/140‑1 (MQ=255)

                                                                                                       tACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAACAGCAGCCTATGTACCCATTGTCAGCGCGCCCCGGAACACAAGCCCGCCGGTCTTATCTTTATTTTGTCCCATCAGATTAAcgc                                     >  1:2373691/1‑138 (MQ=255)

                                                                                                       tACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTGTGCAACACCAGCATATGTACCCATTGTCAGCGCCCACGGAAACACATGCCCGCCCGTATAAAATTTATTTTCTCCAATCCGAttaaata                                     >  1:1822109/1‑135 (MQ=255)

                                                                                                        aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTGTGCAACACCAGCAAATTTACCCATTGTCAGCGCGCCCCGAAACACATGCCCCCCCGTATAAACTGTATTTTTTGCAATAAGATTAAcact                                    >  1:2075220/1‑140 (MQ=255)

                                                                                                                      gTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATTTACCCATTGTCAGCGCGCACGGAAAAACAAGACCGCCCGTATAAACTGTAAGTTCTCCCAAAAGATTAAATATTACAGGTGCGaaaa                      >  2:1940913/1‑140 (MQ=255)

                                                                                                                      gTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGaaaa                      <  2:665187/140‑1 (MQ=255)

                                                                                                                      gTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACAGCAGCATATGTAACCAATGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCACATTAACTCTTCCACGTGCGaaaa                      >  2:2432048/1‑140 (MQ=255)

                                                                                                                       tCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACACCAGCATATGTACCCAATGTCACCGCGCACCGAAACACATGCCCGCCCGTATAAAATGTATTTTCTCCCATCAGATTCACACATCCAGGTGCGaaaaa                     >  2:1281724/1‑140 (MQ=255)

                                                                                                                       tCTGAATACATCCGGACAACCCCGTCGTTTATTTATTCCACAGCAGCATAATTAACCATTGTCAGCGCGCACGGAAACAAAATCCCCCCGTTATAATATTTATTTTCTCCAATCAGATTCAATATTACAGGTGCGaaaaa                     >  2:1073657/1‑140 (MQ=255)

                                                                                                                        cTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGaaaaaa                    <  2:731584/140‑1 (MQ=255)

                                                                                                                        cTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGaaaaaa                    <  2:1108487/140‑1 (MQ=255)

                                                                                                                              aCATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATATTCTGTATGTTCTCCAATCAGTTTCACTCTTCCAGGTGCTAAAAAAAAcctt              <  2:89059/140‑3 (MQ=255)

                                                                                                                              aCATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGt              <  2:1426356/140‑1 (MQ=255)

                                                                                                                              aCATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGt              <  1:813921/140‑1 (MQ=255)

                                                                                                                                aTCCGGACAACCCCGTCGTGTCTTTCTGCAACAGCAGCATATGTACCCACTGTCAGCCCGGCCGGAAACACATGCCCGCCGGTATAATCTGTATGGTCGCCCATCACATTCACACGGCCAGGGGCGAAAAAAAAGCggga            >  2:1911250/1‑137 (MQ=255)

                                                                                                                                 tCCGGACAACCCCGTCGTTTGTTTCTGCAACAGCAGCATATGTAACCATTGTCACCGCGCACGGAAACACATGCCCCCCGTTATAATGTGTATGTTGTCCAATAAGATTAAATCTTCCAGGTGCGAAAAAAAAACGTAAc           >  2:124585/1‑140 (MQ=255)

                                                                                                                                 tCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAc           <  2:1302242/140‑1 (MQ=255)

                                                                                                                                   cGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAccc         <  2:232135/140‑1 (MQ=255)

                                                                                                                                     gACAACCCCGTCGTTTGTTTCTGCAACAGCACCAAATGTACCCCTTGGCAGCGCGCCCGGAAACACCAGCCCGCCGGTATAATCTGTATGTTCTCCAAACAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAcccca       >  2:199519/1‑139 (MQ=255)

                                                                                                                                     gACAACCCCGTCGTTTCTTTCTGCAACACCAGCATATTTACCCAATGTGAGCGCCCACCGTAACACATGCCCGCCCTTATAATATGTATTTTGTCCAATAAGATTGACACTTCCAGCTGGGAAAAAAAACCCGAAcccca       >  2:620688/1‑139 (MQ=255)

                                                                                                                                     gACAACCCCGTCGTTTCGTTGTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAAGCCCGCCGTTATAATATGGATGTTCTCCCATAAGATTAACACTTCCAGGTGCGAAAAAAAACCGGAAcccca       >  2:1003931/1‑139 (MQ=255)

                                                                                                                                     gACAACCCCGTCGTTTCGTTCAGCCACAGCAGCAGATGTACCCCTTGTCAGCGCGCACGGCAACACAAGCCCGCCCGTATAATCTGTATGTTCTCCAAACCGATTCACTCTTCCACGTGCGAAAAAAAAACGTCAccccg       >  2:1808139/1‑139 (MQ=255)

                                                                                                                                     gACAACCCCGTCGTGTCTTTGTGCAACAGCAGCATATGTACCCAATGTAAGCCCCCACCGAAACACAAGCCCGCCCGTAAAAAAAGTATTTTTTACAAAAACAATAACAATTACCAGTGCGAAAAAAAAACGTAAacccg       >  2:427282/1‑139 (MQ=255)

AATAGCCCCTTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTT  >  NZ_CP034943/3746746‑3747023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`