Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,991,699 T→C 29.0% K46E (AAA→GAA)  walH ← WalRK two‑component regulatory system regulator WalH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,991,6990TC29.0% 327.8 / 168.4 216K46E (AAA→GAA) walHWalRK two‑component regulatory system regulator WalH
Reads supporting (aligned to +/- strand):  ref base T (71/81);  new base C (29/32);  total (102/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

TGAGGCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAATATCGTTTTTATATTTTCACGCTTCATCC  >  NZ_CP034943/3991563‑3991836
                                                                                                                                        |                                                                                                                                         
tGAGGCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCCGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTtctc                                                                                                                                        <  1:625726/140‑1 (MQ=255)
tGAGGCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTtctc                                                                                                                                        <  2:690371/140‑1 (MQ=255)
tGAGGCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTtctc                                                                                                                                        <  2:1770236/140‑1 (MQ=255)
tGAGGCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTcctc                                                                                                                                        <  2:2402990/140‑1 (MQ=255)
   ggCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACg                                                                                                                                     <  1:728388/140‑1 (MQ=255)
   ggCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACg                                                                                                                                     <  1:1903037/140‑1 (MQ=255)
   ggCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTATTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACg                                                                                                                                     <  1:1636649/140‑1 (MQ=255)
   ggCAACTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACg                                                                                                                                     <  2:1292821/140‑1 (MQ=255)
    gCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGc                                                                                                                                    <  2:422858/140‑1 (MQ=255)
    gCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGc                                                                                                                                    <  2:1725073/140‑1 (MQ=255)
    gCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGc                                                                                                                                    <  1:492173/140‑1 (MQ=255)
    gCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGc                                                                                                                                    <  2:67803/140‑1 (MQ=255)
    gCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGc                                                                                                                                    <  1:1693733/140‑1 (MQ=255)
     cAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGca                                                                                                                                   >  1:163275/1‑140 (MQ=255)
       aGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGcaca                                                                                                                                 >  1:1962836/1‑140 (MQ=255)
         tCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGt                                                                                                                               <  1:638939/140‑1 (MQ=255)
          cagaccagacTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTa                                                                                                                              <  1:1171908/140‑1 (MQ=255)
          cagaccagacTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTa                                                                                                                              <  2:1264076/140‑1 (MQ=255)
          cagaccagacTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTa                                                                                                                              <  2:163275/140‑1 (MQ=255)
          cagaccagacTACTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTa                                                                                                                              <  2:593707/140‑1 (MQ=255)
             accagacTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGAc                                                                                                                           >  1:67377/1‑140 (MQ=255)
             accacacTTCTTAATAGAGGGATTCATCAACCACTTTGTAATGAGCGCAGTCATCATGGATAAACATGTCCATCGGTCTTACCGTTTCAGACAACTTATCTGTGTTTTTTTCTATTTTATGTTTCTCACGCACAGAAGAc                                                                                                                           >  2:1678032/1‑140 (MQ=255)
              ccagacTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGAct                                                                                                                          <  2:2282537/140‑1 (MQ=255)
               cagacTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGActc                                                                                                                         <  2:2414957/140‑1 (MQ=255)
               cagacTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGActc                                                                                                                         <  2:76644/140‑1 (MQ=255)
                 gacTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTg                                                                                                                       <  2:400007/140‑1 (MQ=255)
                  acTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGt                                                                                                                      >  1:1944621/1‑140 (MQ=255)
                   cttcttCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGtt                                                                                                                     >  1:1928658/1‑140 (MQ=255)
                   cttcttCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGtt                                                                                                                     <  2:61825/140‑1 (MQ=255)
                      cttcATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACCTGTCCCTCGGTCTTACCGTTTCAGACAAGTTGGGGGTGTTTTGTTCGCTTTTATGTTCCTCAGGCCCCGTAGCCTCTGtgggt                                                                                                                  >  2:1885386/1‑136 (MQ=255)
                       ttcATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTtgatg                                                                                                                 >  1:1754794/1‑140 (MQ=255)
                            atagGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCACTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATTTTTCTCACGCACAGTAGACTCTGTTGATGATGAg                                                                                                            >  2:1139916/3‑140 (MQ=255)
                            agagGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAg                                                                                                            >  2:5670/1‑140 (MQ=255)
                            agagGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTGTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAg                                                                                                            >  2:336243/1‑140 (MQ=255)
                              agGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGa                                                                                                                            <  1:1522978/122‑1 (MQ=255)
                              agGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGcc                                                                                                          >  1:1049849/1‑140 (MQ=255)
                              agGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGcc                                                                                                          >  1:356461/1‑140 (MQ=255)
                                tgCTTAATCATCCACTTTGTAAAGAGCGCCGTCATCATTGATTAACATGTCCCTCCGTCTTACCGGTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGGTCATGATTAGCCtt                                                                                                        >  2:1215552/2‑140 (MQ=255)
                                ggCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTg                                                                                                                       <  2:1504017/125‑1 (MQ=255)
                                ggCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCtt                                                                                                        >  1:552316/1‑140 (MQ=255)
                                ggCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCtt                                                                                                        >  1:290460/1‑140 (MQ=255)
                                ggCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCtt                                                                                                        >  2:2070035/1‑140 (MQ=255)
                                ggCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTACTCACGCACAGTAGACTCTGTTGATGATGAGCCtt                                                                                                        >  2:362185/1‑140 (MQ=255)
                                ggCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTATTGGGTGTTTTTTTCGATTTTATGTTCCTCAAGCACAGTAGAATCTGTTGATGATGAGCCtt                                                                                                        >  2:2298564/1‑140 (MQ=255)
                                 gCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTc                                                                                                       >  2:826321/1‑140 (MQ=255)
                                          tcCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGtt                                                                                              >  1:1084976/1‑140 (MQ=255)
                                          tcCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGtt                                                                                              >  1:2265518/1‑140 (MQ=255)
                                            cACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAATTTTg                                                                                            >  2:1388770/1‑140 (MQ=255)
                                            cACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTg                                                                                            >  2:48338/1‑140 (MQ=255)
                                               tttGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGActc                                                                                                                         >  1:1796217/1‑108 (MQ=255)
                                                 tGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTgg                                                                                       >  2:1101880/1‑140 (MQ=255)
                                                 tGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTgg                                                                                       >  2:1867314/1‑140 (MQ=255)
                                                  gTAATGAGCGCCGTCATCAGGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTGGATTTTATGTTCCTCACGCACAGTAGACTCTTTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGa                                                                                      <  2:713965/140‑1 (MQ=255)
                                                   tactGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTTTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGaa                                                                                     <  2:2183176/137‑1 (MQ=255)
                                                   tAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGaa                                                                                     <  1:1014895/140‑1 (MQ=255)
                                                   tAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGaa                                                                                     <  2:905772/140‑1 (MQ=255)
                                                   tAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGaa                                                                                     <  1:557908/140‑1 (MQ=255)
                                                   tAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGGTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGaa                                                                                     <  2:2420999/140‑1 (MQ=255)
                                                        aGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTcc                                                                                >  2:648606/1‑140 (MQ=255)
                                                        aGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTcc                                                                                >  1:808552/1‑140 (MQ=255)
                                                        aGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTcc                                                                                >  1:2414995/1‑140 (MQ=255)
                                                            ccGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCatat                                                                            <  2:776681/140‑1 (MQ=255)
                                                                catcatGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATcccc                                                                        <  1:1413055/140‑1 (MQ=255)
                                                                     tGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGt                                                                   <  1:1316698/140‑1 (MQ=255)
                                                                       gATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGaa                                                                                     <  1:1972188/120‑1 (MQ=255)
                                                                       gATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGaa                                                                                     >  2:1972188/1‑120 (MQ=255)
                                                                         tAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAac                                                               >  2:2278462/1‑140 (MQ=255)
                                                                           aaCATGTCCCTCGGTCTTACCGTTTCAGACACCTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAacac                                                             >  2:1818375/1‑140 (MQ=255)
                                                                           aaCATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAacac                                                             >  1:1460493/1‑140 (MQ=255)
                                                                                  gacTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTTGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACATGACtg                                                      <  2:1610722/138‑1 (MQ=255)
                                                                                     tCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACtgatg                                                   <  1:136621/140‑1 (MQ=255)
                                                                                     tCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACtgatg                                                   <  2:1003606/140‑1 (MQ=255)
                                                                                     tCGGTCTTACAGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACtgatg                                                   <  2:1407300/140‑1 (MQ=255)
                                                                                      cGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGGTGAGCCTTCAGAAAAGTTTGGCTGGTACGTCCATATCCCCCACGTAAACACAAGACtgatga                                                  >  1:2308452/1‑140 (MQ=255)
                                                                                      cGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACtgatga                                                  >  1:1736342/1‑140 (MQ=255)
                                                                                      cGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACtgatga                                                  >  1:1109782/1‑140 (MQ=255)
                                                                                      cGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACtgatga                                                  >  1:844017/1‑140 (MQ=255)
                                                                                       ggTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACtgatgat                                                 >  1:1211038/1‑140 (MQ=255)
                                                                                        gTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACtgatgatg                                                >  1:327535/1‑140 (MQ=255)
                                                                                            tACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATCTCCCCCCCGTAAACACAAGACTGATGATGACGa                                            <  1:1132545/140‑1 (MQ=255)
                                                                                            tACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGa                                            <  1:2162250/140‑1 (MQ=255)
                                                                                            tACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGa                                            <  1:2065368/140‑1 (MQ=255)
                                                                                              ccGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGt                                          <  1:113014/140‑1 (MQ=255)
                                                                                                 tttCAGACATCTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTAcc                                       <  1:2023049/140‑1 (MQ=255)
                                                                                                 tttAAGACAACTTTTGGGTGTTTTTTTCTATTTTATGTTTCTCACGCACAGTAGACTCTGTTTATGATGAGCCTTCAGAAAAGTTTGGCTGGAAAGTCCATATCCCCCAAGTAAACACAAGACTGATGATGACGAGTAcc                                       <  2:455866/140‑1 (MQ=255)
                                                                                                   tCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCgt                                     <  1:536081/140‑1 (MQ=255)
                                                                                                   tCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCgt                                     <  1:1818445/140‑1 (MQ=255)
                                                                                                           cTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAata                             >  1:20830/1‑140 (MQ=255)
                                                                                                                    gtTTTTTTCGATTGTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAATATCGTTTTta                    <  2:2383818/140‑1 (MQ=255)
                                                                                                                               ttttATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAATATCGTTTTTATATTTTCACGc         <  2:1441144/140‑1 (MQ=255)
                                                                                                                               ttttATGTTACTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAATATCGTTTTTATATTTTCACGc         <  2:514965/140‑1 (MQ=255)
                                                                                                                                tttATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAATATCGTTTTTATATTTTCACGCt        <  1:926661/140‑1 (MQ=255)
                                                                                                                                 ttATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCCCGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAATATCGTTTTTATATTTTCACGCtt       <  2:137872/140‑1 (MQ=255)
                                                                                                                                 ttATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAATATCGTTTTTATATTTTCACGCtt       <  2:1928658/140‑1 (MQ=255)
                                                                                                                                 ttATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAAGATCGTTTTTATATTTTCACGCtt       <  1:438327/140‑1 (MQ=255)
                                                                                                                                 ttATGTTCCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAATGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAATATCGTTTTTATATTTTCACGCtt       <  1:313068/140‑1 (MQ=255)
                                                                                                                                      tttCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTCAACACAAGACTGATGATGACGAGTACCGTAAGTAATATCGTTTTTATATTTTCACGCTTCATcc  <  2:259459/140‑1 (MQ=255)
                                                                                                                                      tttCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAATATCGTTTTTATATTTTCACGCTTCATcc  <  2:1538833/140‑1 (MQ=255)
                                                                                                                                      tttCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAATATCGTTTTTATATTTTCACGCTTCATcc  <  1:200357/140‑1 (MQ=255)
                                                                                                                                      tttCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAATATCGTTTTTATATTTTCACGCTTCATcc  <  2:1084976/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                         
TGAGGCAAGTCAGACCAGACTTCTTCATAGAGGGCTTCATCATCCACTTTGTAATGAGCGCCGTCATCATGGATAAACATGTCCCTCGGTCTTACCGTTTCAGACAACTTTTGGGTGTTTTTTTCGATTTTATGTTTCTCACGCACAGTAGACTCTGTTGATGATGAGCCTTCAGAAAAGTTTGGCTGGAACGTCCATATCCCCCACGTAAACACAAGACTGATGATGACGAGTACCGTAAGTAATATCGTTTTTATATTTTCACGCTTCATCC  >  NZ_CP034943/3991563‑3991836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: