Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 480,287 A→G 6.0% V140V (GTA→GTG EO946_RS02465 → glycosyltransferase family 2 protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943480,2870AG 6.0% 538.6 / 16.1 167V140V (GTA→GTGEO946_RS02465glycosyltransferase family 2 protein
Reads supporting (aligned to +/- strand):  ref base A (48/109);  new base G (3/7);  total (51/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 169 total aligned reads displayed.

GTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACGAAGTTTATT  >  NZ_CP034943/480151‑480425
                                                                                                                                        |                                                                                                                                          
gTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTatat                                                                                                                                         <  1:948708/140‑1 (MQ=255)
 tGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATg                                                                                                                                        <  1:2018176/140‑1 (MQ=255)
 tGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATg                                                                                                                                        <  2:693315/140‑1 (MQ=255)
 tGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATg                                                                                                                                        <  1:2046599/140‑1 (MQ=255)
 tGATATCGTCAATGAATTTTCCGAGAAATACGATTTCATCAAAATGGTGATTACGAAGCCGCCAAATGCAGGAAAAGGAAAATCCTCTGCACTTAACTCGGGTTTTGCCGAATCGAACGGCGATGTGATCTGTGTGTATg                                                                                                                                        >  2:2234693/1‑140 (MQ=255)
 tGATATCGTCAATGAATTTTCCGAGAAATACGATTTCATCAAAATGGTGATTACGAAGCCGCCAAATGCAGGAAAAGGAAAATCCTCTGCACTTAACTCGGGTTTTGCCGAATCGAACGGCGATGTGATCTGTGTGTATg                                                                                                                                        >  2:774784/1‑140 (MQ=255)
 tGATATCGTCAATGAATTTTCCGAGAAATACGATTTCATCAAAATGGTGATTACGAAGCCGCCAAATGCAGGAAAAGGAAAATCCTCTGCACTTAACTCGGGTTTTGCCGAATCGAACGGCGATGTGATCTGTGTGTATg                                                                                                                                        >  2:337913/1‑140 (MQ=255)
 tGATATCGTCAATGAATGTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATg                                                                                                                                        <  2:2433552/140‑1 (MQ=255)
     atCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGc                                                                                                                                    >  1:1636737/1‑140 (MQ=255)
     atCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGc                                                                                                                                    >  1:2088631/1‑140 (MQ=255)
      tCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGcc                                                                                                                                   >  1:1966649/1‑140 (MQ=255)
      tCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGcc                                                                                                                                   <  1:606107/140‑1 (MQ=255)
      tCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGcc                                                                                                                                   >  1:1339589/1‑140 (MQ=255)
      tCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGcc                                                                                                                                   <  1:566044/140‑1 (MQ=255)
          cAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGaca                                                                                                                               >  1:493120/1‑140 (MQ=255)
           aaTGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGacaa                                                                                                                              <  1:2212856/140‑1 (MQ=255)
           aaTGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGacaa                                                                                                                              <  2:441544/140‑1 (MQ=255)
           aaTGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGacaa                                                                                                                              <  2:938315/140‑1 (MQ=255)
                         gacaTACGATTTTATCAAAATGGTGATCACAAAACCGCCGAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGCCGCCGACAACACCCCTGAGaaaa                                                                                                                <  2:2121749/137‑1 (MQ=255)
                         gaAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGaaaa                                                                                                                <  2:1990496/140‑1 (MQ=255)
                         gaAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGaaaa                                                                                                                <  2:1603052/140‑1 (MQ=255)
                            aTACGATTTTATCAAAATGGTGATCACAACACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATgg                                                                                                             <  2:275222/140‑1 (MQ=255)
                            aTACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATgg                                                                                                             <  2:1624595/140‑1 (MQ=255)
                            aTACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATgg                                                                                                             <  2:1124876/140‑1 (MQ=255)
                            aTACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATgg                                                                                                             <  2:1124885/140‑1 (MQ=255)
                            aTACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATgg                                                                                                             <  1:1256500/140‑1 (MQ=255)
                            aTACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATgg                                                                                                             <  2:1851098/140‑1 (MQ=255)
                            aTACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATgg                                                                                                             <  2:1593949/140‑1 (MQ=255)
                            aTACGATTTCATCAAAATGGTGATTACGAAGCCGCCAAATGCAGGAAAAGGAAAATCCTCTGCACTTAACTCGGGTTTTGCCGAATCGAACGGCGATGTGATCTGTGTGTATGACGCCGACAACACGCCTGAGAAAATgg                                                                                                             <  2:682295/140‑1 (MQ=255)
                            aTACGATTTCATAAAAATGGTGATTACGAAGCCGCCAAATGCAGGAAAAGGAAAATCCTCTGCACTTAACTCGGGTTTTGCCGTATCGAACGGCGATGTGATCTGTGTGTATGACGCCGACAACACGCCTGAGAAAATgg                                                                                                             <  2:319588/140‑1 (MQ=255)
                              aagATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGcc                                                                                                           <  1:1445471/138‑1 (MQ=255)
                              aCGATTTTATCAAAATGGTGATCACAAAACCGCCTACCGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGcc                                                                                                           <  2:1926079/140‑1 (MQ=255)
                              aCGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGcc                                                                                                           <  1:1417038/140‑1 (MQ=255)
                              aCGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGcc                                                                                                           <  2:2075274/140‑1 (MQ=255)
                              aCGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGcc                                                                                                           <  2:2061970/140‑1 (MQ=255)
                              aCGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGcc                                                                                                           <  2:2349688/140‑1 (MQ=255)
                              aCGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAAGCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGcc                                                                                                           <  1:35457/140‑1 (MQ=255)
                                 aTTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCgtg                                                                                                        <  1:2224810/140‑1 (MQ=255)
                                   tttATCAAAATGGTGATCACAAACCCGCCTAACGCCGGACAAGGCAAATCCTCTGCACTGAACTCTGGACTTGCCTAATCGAACGCCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTa                                                                                                      <  1:2075568/140‑1 (MQ=255)
                                   tttATCAAAATGGTGATCACAAAACCGCCTACCGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTa                                                                                                      <  1:1100513/140‑1 (MQ=255)
                                   tttATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTa                                                                                                      <  1:764578/140‑1 (MQ=255)
                                   tttATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTa                                                                                                      <  1:1831337/140‑1 (MQ=255)
                                   tgtATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACGGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTAAATGACGCCTACAACACCCCTGAGAAAATGGCCGTGTa                                                                                                      <  1:1831305/138‑1 (MQ=255)
                                    ttATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTAt                                                                                                     >  1:670236/1‑140 (MQ=255)
                                    ttATCAAAATGGTGATCAAAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTAt                                                                                                     <  1:244588/140‑1 (MQ=255)
                                     tATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTAtt                                                                                                    <  1:390501/140‑1 (MQ=255)
                                     tATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTAtt                                                                                                    <  1:2263929/140‑1 (MQ=255)
                                     tATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTAtt                                                                                                    <  1:1016947/140‑1 (MQ=255)
                                     gaTCAAAAGGGTGATCACAAACCCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTAtt                                                                                                    <  1:2264109/139‑1 (MQ=255)
                                         aaaaTGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCt                                                                                                <  1:149156/140‑1 (MQ=255)
                                               gTGATCACAAAACCGCCTACCGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCt                                                                                          <  2:573366/140‑1 (MQ=255)
                                               gTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCt                                                                                          <  1:301959/140‑1 (MQ=255)
                                               gTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCt                                                                                          <  1:508559/140‑1 (MQ=255)
                                               gTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCt                                                                                          <  1:2123006/140‑1 (MQ=255)
                                                tGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTc                                                                                         >  1:1054972/1‑140 (MQ=255)
                                                       aaaaCCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCtga                                                                                  >  2:1330303/1‑140 (MQ=255)
                                                           ccGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGatgaa                                                                              <  2:2165847/140‑1 (MQ=255)
                                                           ccGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGatgaa                                                                              <  1:345962/140‑1 (MQ=255)
                                                            cGCCAAATGCAGGAAAAGGAAAATCCTCTGCACTTAACTCGGGTTTTGCCGAATCGAACGGCGATGTGATCTGTGTGTATGACGCCGACAACACGCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGatgaat                                                                             <  1:774784/140‑1 (MQ=255)
                                                              ccTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGatgaatga                                                                           >  2:1904856/1‑140 (MQ=255)
                                                               cTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGAc                                                                          >  2:14609/1‑140 (MQ=255)
                                                               cTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGAc                                                                          >  2:1567955/1‑140 (MQ=255)
                                                                       ggAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAaggc                                                                  <  1:1330303/140‑1 (MQ=255)
                                                                       ggAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAaggc                                                                  <  1:173598/140‑1 (MQ=255)
                                                                       cgAAAAGGCAAATCCTCTGCACGGAACTCTGGCTTTGCCGAATCGACCGGCGATGTGATCTGTGTATATGCCGCCGACCACACCCCGGAGCAAATGGCCGTGTATTCCCTCGTGCTCGGCCTGATGAATGACGAAAcggc                                                                  <  2:2313847/139‑1 (MQ=255)
                                                                        gAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAaggca                                                                 >  2:1698556/1‑140 (MQ=255)
                                                                             ggCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGgcgc                                                            <  1:1342597/140‑1 (MQ=255)
                                                                             ggCAAATCCTCTGCACTGAACTCGGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGgcgc                                                            <  1:1697202/140‑1 (MQ=255)
                                                                              gCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGcc                                                           <  2:1966649/140‑1 (MQ=255)
                                                                              gCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGcc                                                           <  2:1339589/140‑1 (MQ=255)
                                                                              gCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGcc                                                           <  2:376441/140‑1 (MQ=255)
                                                                                  aTCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTgg                                                       >  2:1523247/1‑140 (MQ=255)
                                                                                  aTCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTgg                                                       <  1:1586047/140‑1 (MQ=255)
                                                                                  aTCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTgg                                                       >  2:1119350/1‑140 (MQ=255)
                                                                                       ctGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACACCACCCCTGAGAAAATGGCCGTGGATTACCTCGTGCTCGGCCTGATGACTGACGAAAAGGCAGGCGCCGTGGTTggg                                                  >  2:13768/1‑140 (MQ=255)
                                                                                       ctGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTggg                                                  <  2:1886842/140‑1 (MQ=255)
                                                                                        tGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGa                                                 >  1:1791308/1‑140 (MQ=255)
                                                                                             tGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCt                                                                                                                       <  2:1435686/65‑1 (MQ=255)
                                                                                             tGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCt                                                                                                                       >  1:1435686/1‑65 (MQ=255)
                                                                                               aaCTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATCTGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCcg                                          <  2:456328/140‑1 (MQ=255)
                                                                                               aaCTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCcg                                          <  2:2059229/140‑1 (MQ=255)
                                                                                               aaCTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCcg                                          >  1:1991256/1‑140 (MQ=255)
                                                                                               aaCTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCcg                                          <  2:550734/140‑1 (MQ=255)
                                                                                               aaCTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCcg                                          >  1:1556682/1‑140 (MQ=255)
                                                                                                    tGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGtca                                     >  1:681179/1‑140 (MQ=255)
                                                                                                          tGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATg                               <  2:2279945/140‑1 (MQ=255)
                                                                                                           gCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGc                              <  2:1752131/140‑1 (MQ=255)
                                                                                                                              tgATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACg           <  1:703138/140‑1 (MQ=255)
                                                                                                                              tgATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACg           <  1:991868/140‑1 (MQ=255)
                                                                                                                                 tCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACGAAg        <  1:1256302/140‑1 (MQ=255)
                                                                                                                                   tgtgTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACGAAGtt      >  2:1484405/1‑140 (MQ=255)
                                                                                                                                     tgtATATGACGCCGACAACACCCCTGAGAAACTGGCCGGGTATTACCTCGTGCTCGGCCTGATGAATGACGACAAGGCAGGCGCCGTGGTTGGGACATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACGAAGTtta    >  1:892960/1‑140 (MQ=255)
                                                                                                                                     tgtATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACGTAGTtta    >  1:2419944/1‑140 (MQ=255)
                                                                                                                                     tgtATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACGAAGTtta    >  2:2174789/1‑140 (MQ=255)
                                                                                                                                     tgtATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACGAAGTtta    >  1:2293582/1‑140 (MQ=255)
                                                                                                                                     tgtATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACGAAGTtta    >  2:1203824/1‑140 (MQ=255)
                                                                                                                                      gtATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACGAAGTttat   <  1:1942127/140‑1 (MQ=255)
                                                                                                                                      gtATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACGAAGTttat   <  1:1475545/140‑1 (MQ=255)
                                                                                                                                      gtATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACGAAGTttat   <  1:2044167/140‑1 (MQ=255)
                                                                                                                                       tataTGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACGAAGTttatt  <  2:802206/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
GTGATATCGTCAATGAATTTTCCGAGAAATACGATTTTATCAAAATGGTGATCACAAAACCGCCTAACGCCGGAAAAGGCAAATCCTCTGCACTGAACTCTGGATTTGCCGAATCGAACGGCGATGTGATCTGTGTATATGACGCCGACAACACCCCTGAGAAAATGGCCGTGTATTACCTCGTGCTCGGCCTGATGAATGACGAAAAGGCAGGCGCCGTGGTTGGGAAATTCCGCGTCATCAATGCGGCAAAGACGCTGCTGACGAAGTTTATT  >  NZ_CP034943/480151‑480425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: