Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 506,292 G→C 5.6% V277V (GTG→GTC EO946_RS02595 → cation diffusion facilitator family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943506,2920GC 5.6% 522.8 / 16.0 157V277V (GTG→GTCEO946_RS02595cation diffusion facilitator family transporter
Reads supporting (aligned to +/- strand):  ref base G (83/64);  new base C (5/4);  total (88/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

AAAGATGTGAAAGCGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCATTGGCGCTGTGAAGGTGAGCTGTTTTTTCA  >  NZ_CP034943/506155‑506430
                                                                                                                                         |                                                                                                                                          
aaaGATGTGAAAGCGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGAc                                                                                                                                          <  1:2294020/140‑1 (MQ=255)
aaaGATGTGAAAGCGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGAc                                                                                                                                          <  1:378106/140‑1 (MQ=255)
aaaGATGTGAAAGAGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTAAAGTAGAAGTAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGAc                                                                                                                                          <  1:2194528/140‑1 (MQ=255)
   gATGTGAAAGCGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACtca                                                                                                                                       <  2:1626698/140‑1 (MQ=255)
      gtgAAAGCGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACTGTAGAAGACGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAAGAAAACATGAACACGATGTGACtcatgt                                                                                                                                    >  1:1786731/1‑140 (MQ=255)
      gtgAAAGCGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACtcatgt                                                                                                                                    >  1:1266435/1‑140 (MQ=255)
       tgAAAGCGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACtcatgtt                                                                                                                                   <  1:1573420/140‑1 (MQ=255)
         aaaGCGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACtcatgttca                                                                                                                                 <  2:836695/140‑1 (MQ=255)
         aaaGCGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACtcatgttca                                                                                                                                 <  2:1137885/140‑1 (MQ=255)
         aaaGCGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACtcatgttca                                                                                                                                 <  2:38407/140‑1 (MQ=255)
             cGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACtcatgttcatgtt                                                                                                                             <  1:1026072/140‑1 (MQ=255)
             cGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACtcatgttcatgtt                                                                                                                             <  1:1590544/140‑1 (MQ=255)
             cGAGGTATCTAGGAAGCACAGGTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACtcatgttcatgtt                                                                                                                             <  2:2052451/140‑1 (MQ=255)
               aGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACtcatgttcatgttca                                                                                                                           >  2:1276743/1‑140 (MQ=255)
                           aGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTga                                                                                                               <  1:1544804/140‑1 (MQ=255)
                           aGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTga                                                                                                               <  2:1398557/140‑1 (MQ=255)
                           aGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTga                                                                                                               >  1:450759/1‑140 (MQ=255)
                           aGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTga                                                                                                               <  1:1276743/140‑1 (MQ=255)
                           aGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTga                                                                                                               >  1:702017/1‑140 (MQ=255)
                           aGCACAGGTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTga                                                                                                               <  1:2231223/140‑1 (MQ=255)
                            gtacaGTTCATATCGAGATGGTGATTACGGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCGGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTCACTCATGTTCATGTTCATGTAGAGCCTgac                                                                                                              >  2:2075813/3‑140 (MQ=255)
                            gtacaGTTCATATCGAGATGGTGATTACGGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCGGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTCACTCATGTTCATGTTCATGTAGAGCCTgac                                                                                                              >  2:860872/3‑140 (MQ=255)
                            gCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTgac                                                                                                              >  1:1458836/1‑140 (MQ=255)
                            gCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTgac                                                                                                              >  1:2341698/1‑140 (MQ=255)
                             cacaGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTgacg                                                                                                             >  1:1648179/1‑140 (MQ=255)
                                aGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACa                                                                                                          <  1:144796/140‑1 (MQ=255)
                                aGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACa                                                                                                          <  1:1922052/140‑1 (MQ=255)
                                aGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACa                                                                                                          <  2:1519612/140‑1 (MQ=255)
                                aGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACa                                                                                                          <  1:797617/140‑1 (MQ=255)
                                aGTTCATATCGAAAGGGTCATTCCAGGTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACa                                                                                                          <  1:1945120/140‑1 (MQ=255)
                                  ttCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACtc                                                                                                                                        <  2:1944576/108‑1 (MQ=255)
                                       atCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATa                                                                                                   >  1:869542/1‑140 (MQ=255)
                                           aaaTGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCCTGTAGAGCCTGACGACATAAAATAAGGc                                                                                               >  2:1430994/1‑140 (MQ=255)
                                           aaaTGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGc                                                                                               >  2:2248788/1‑140 (MQ=255)
                                           aaaTGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGc                                                                                               >  2:954541/1‑140 (MQ=255)
                                           aaaTGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGc                                                                                               >  2:396221/1‑140 (MQ=255)
                                            aaTGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCt                                                                                              >  1:958157/1‑140 (MQ=255)
                                            aaTGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCt                                                                                              >  2:1210200/1‑140 (MQ=255)
                                                gTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGttt                                                                                          <  1:2271503/140‑1 (MQ=255)
                                                gTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGttt                                                                                          <  1:1719895/140‑1 (MQ=255)
                                                gTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGttt                                                                                          >  2:1651502/1‑140 (MQ=255)
                                                      aCAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGaa                                                                                    <  2:1780453/140‑1 (MQ=255)
                                                      aCAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGaa                                                                                    <  2:2341698/140‑1 (MQ=255)
                                                      aCAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGaa                                                                                    <  2:2009039/140‑1 (MQ=255)
                                                       cAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAAt                                                                                   <  1:1090096/140‑1 (MQ=255)
                                                       cAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGAAATAAAATAAGGCTTTTTAACGAAt                                                                                   <  1:241450/140‑1 (MQ=255)
                                                          tttaTCCTAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATTTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAg                                                                                <  2:109291/137‑1 (MQ=255)
                                                          ttGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGCCAAAATAAAACATGAAAACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTTTTTAACGAATTAg                                                                                <  1:2362574/140‑1 (MQ=255)
                                                           tGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAACCATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGc                                                                               <  1:2197874/140‑1 (MQ=255)
                                                           tGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGc                                                                               <  1:1447742/140‑1 (MQ=255)
                                                             aTCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGTCCGATGAAGTAGAATGCAAAATAAATCATGAACACGATGTGACTCATGTTCATGATAATGTATAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCtt                                                                             >  1:847837/1‑140 (MQ=255)
                                                             aTCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTa                                                                                         <  1:1121855/128‑1 (MQ=255)
                                                             aTCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCtt                                                                             >  1:691164/1‑140 (MQ=255)
                                                             aTCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCtt                                                                             >  1:658357/1‑140 (MQ=255)
                                                             aTCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCtt                                                                             >  1:1985861/1‑140 (MQ=255)
                                                                    gCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGtt                                                                      >  1:1783255/1‑140 (MQ=255)
                                                                            tAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAAt                                                                                   <  1:1912792/119‑1 (MQ=255)
                                                                            tAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAAt                                                                                   >  2:1914362/1‑119 (MQ=255)
                                                                               aagaagGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACtcatgttcatg                                                                                                                               <  1:462576/72‑1 (MQ=255)
                                                                               aagaagGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAAt                                                           >  1:602500/1‑140 (MQ=255)
                                                                               aagaagGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAAt                                                           >  2:1934327/1‑140 (MQ=255)
                                                                               aagaagGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAAt                                                           >  1:2388299/1‑140 (MQ=255)
                                                                               aagaagGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTCACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGATTATATGTTCAGATAAAAAt                                                           >  1:2111179/1‑140 (MQ=255)
                                                                                    ggCCATGGAGTGGCAGATGACGTAGAAGACACAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATCTGTTCAGATAAAAATTCAGt                                                      >  1:1358852/1‑140 (MQ=255)
                                                                                    ggCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGt                                                      >  1:1202898/1‑140 (MQ=255)
                                                                                    ggCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGt                                                      >  1:1693790/1‑140 (MQ=255)
                                                                                    ggCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGt                                                      >  2:440985/1‑140 (MQ=255)
                                                                                    ggCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGt                                                      >  1:974034/1‑140 (MQ=255)
                                                                                    ggCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGt                                                      >  2:830841/1‑140 (MQ=255)
                                                                                    ggCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGt                                                      >  1:542418/1‑140 (MQ=255)
                                                                                      ccATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAgg                                                                                                >  2:593464/1‑96 (MQ=255)
                                                                                      ccATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAgg                                                                                                <  1:593464/96‑1 (MQ=255)
                                                                                       cATGGAGTGGCGGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTCACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGACTTAGCTTATATGTTCAGATAAAAATTCAGTCAc                                                   <  1:137294/140‑1 (MQ=255)
                                                                                       cATGGAGTGGCGGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTCACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCAc                                                   <  1:2075813/140‑1 (MQ=255)
                                                                                       cATGGAGTGGCGGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTCACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCAc                                                   <  1:2076319/140‑1 (MQ=255)
                                                                                         tGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCActt                                                 >  1:893213/1‑140 (MQ=255)
                                                                                         tGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCActt                                                 >  2:577739/1‑140 (MQ=255)
                                                                                         tGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCActt                                                 >  2:46526/1‑140 (MQ=255)
                                                                                         tGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCActt                                                 >  2:66575/1‑140 (MQ=255)
                                                                                         tGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCActt                                                 >  1:1398729/1‑140 (MQ=255)
                                                                                         tGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGAAGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCActt                                                 >  2:295391/1‑140 (MQ=255)
                                                                                              tGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCa                                            >  2:1695005/1‑140 (MQ=255)
                                                                                              tGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCa                                            >  2:259407/1‑140 (MQ=255)
                                                                                              tGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGGTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCa                                            >  2:900577/1‑140 (MQ=255)
                                                                                                gCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAgg                                          >  2:2274553/1‑140 (MQ=255)
                                                                                                gCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAgg                                          >  2:2142035/1‑140 (MQ=255)
                                                                                                gCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAgg                                          >  2:1529056/1‑140 (MQ=255)
                                                                                                           agaagaCAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCAt                               <  1:1533362/140‑1 (MQ=255)
                                                                                                           agaagaCAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCAt                               <  1:1538160/140‑1 (MQ=255)
                                                                                                              agaCAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCATTgg                            >  1:1861854/1‑140 (MQ=255)
                                                                                                              agaCAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCATTgg                            <  2:672902/140‑1 (MQ=255)
                                                                                                              agaCAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCATTgg                            >  1:2258110/1‑140 (MQ=255)
                                                                                                                acaacaTAAAACATGAACAAGATGTGACTCATCTTCATGTTCATGTAGAGCCTGACGACATAAAATAACGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCATTGgcg                          <  1:1041430/140‑1 (MQ=255)
                                                                                                                aCAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCATTGgcg                          <  1:437606/140‑1 (MQ=255)
                                                                                                                aCAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCATTGgcg                          <  2:876002/140‑1 (MQ=255)
                                                                                                                aCAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCATTGgcg                          <  1:46526/140‑1 (MQ=255)
                                                                                                                    aaTAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCATTGGCGCtgt                      >  1:908832/1‑140 (MQ=255)
                                                                                                                                      tgtgACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCATTGGCGCTGTGAAGGTGAGCTGtttttt    <  2:1985861/140‑1 (MQ=255)
                                                                                                                                      tgtgACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCATTGGCGCTGTGAAGGTGAGCTGtttttt    >  2:1865305/1‑140 (MQ=255)
                                                                                                                                      tgtgACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCATTGGCGCTGTGAAGGTGAGCTGtttttt    >  2:1865088/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                          
AAAGATGTGAAAGCGAGGTATCTAGGAAGCACAGTTCATATCGAAATGGTCATTACAGTTGATCCGAAGCTTACAGTAGAAGAAGGCCATGGAGTGGCAGATGAAGTAGAAGACAAAATAAAACATGAACACGATGTGACTCATGTTCATGTTCATGTAGAGCCTGACGACATAAAATAAGGCTGTTTAACGAATTAGCTTATATGTTCAGATAAAAATTCAGTCACTTCTTCAGGCGTTTTGGCATTGGCGCTGTGAAGGTGAGCTGTTTTTTCA  >  NZ_CP034943/506155‑506430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: