Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 523,388 G→A 5.3% A228A (GCG→GCA EO946_RS02705 → RNA‑binding transcriptional accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943523,3880GA 5.3% 357.6 / 6.7 112A228A (GCG→GCAEO946_RS02705RNA‑binding transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base G (52/54);  new base A (3/3);  total (55/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 116 total aligned reads displayed.

CCAGGAAACCTTTAAAAGAGGAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAG  >  NZ_CP034943/523253‑523527
                                                                                                                                       |                                                                                                                                           
ccAGGAAACCTTTAAAAGAGGAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGa                                                                                                                                         >  1:2231671/1‑140 (MQ=255)
ccAGGAAACCTTTAAAAGAGGAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGa                                                                                                                                         >  1:2175640/1‑140 (MQ=255)
ccAGGAAACCTTTAAAAGAGGAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGa                                                                                                                                         >  1:595394/1‑140 (MQ=255)
ccAGGAAACCTTTAAAAGAGGAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGa                                                                                                                                         >  1:1905465/1‑140 (MQ=255)
ccAGGAAACCTTTAAAAGAGGAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGa                                                                                                                                         >  1:1406555/1‑140 (MQ=255)
ccAGGAAACCTTTAAAAGAGGAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAACTCGTTCCGCACAGGGTGCTGGCGATGa                                                                                                                                         >  1:2231573/1‑140 (MQ=255)
    gAAACCTTTAAAAGAGGAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACcg                                                                                                                                     <  2:36472/140‑1 (MQ=255)
         ctatAAAAGAGGAACCCTTAAGTATACAGCCCGAAAATCCGCAGACACAGACGAGAAAAATGTGTATGAAATGTAGTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCgcggcg                                                                                                                                <  1:1860575/137‑1 (MQ=255)
         cTTCAAAAGAGGAACCATTAAGTCTGCAGCCGGAAAGTCGGCAGACACCGATGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTTGTTCCGCATAGGGTGCTGGCAATGAACCgcggcg                                                                                                                                >  1:176306/1‑140 (MQ=255)
                   ggAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTTTGATATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGACGa                                                                                                                      <  2:1813131/140‑1 (MQ=255)
                   ggAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGa                                                                                                                      <  1:1029228/140‑1 (MQ=255)
                   ggAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGa                                                                                                                      <  1:2369992/140‑1 (MQ=255)
                   ggAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGa                                                                                                                      <  2:124176/140‑1 (MQ=255)
                   ggAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGa                                                                                                                      <  2:926264/140‑1 (MQ=255)
                   ggAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGa                                                                                                                      <  2:2396359/140‑1 (MQ=255)
                   ggAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGa                                                                                                                      <  1:1091024/140‑1 (MQ=255)
                   ggAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGa                                                                                                                      <  1:1172135/140‑1 (MQ=255)
                   ggAACCATCAAGTCTACAGCCGGAAAATCTGCAGACACAGACGAGAAAAATGTGTATGAAATCTATTCCGAGTATGAAGCGCCAAGTGCAACAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGa                                                                                                                      <  1:98127/140‑1 (MQ=255)
                          ttAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTa                                                                                                               >  2:207757/1‑140 (MQ=255)
                          ttAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTa                                                                                                               <  2:89332/140‑1 (MQ=255)
                               tCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGCCATTTTAAAGgt                                                                                                          <  1:575412/140‑1 (MQ=255)
                               tCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGgt                                                                                                          <  1:266239/140‑1 (MQ=255)
                               tCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGgt                                                                                                          <  2:500197/140‑1 (MQ=255)
                               tCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGgt                                                                                                          <  1:657538/140‑1 (MQ=255)
                                  aCAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTc                                                                                                       >  1:1857978/1‑140 (MQ=255)
                                      ccGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAACAGGAAGACATTTTAAAGGTGTCTATc                                                                                                   >  1:494304/1‑140 (MQ=255)
                                         gAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAACAGGAAGACCTTTGACAGGTGTCTATCGac                                                                                                >  2:1766834/1‑139 (MQ=255)
                                         gAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGaa                                                                                                >  1:1937475/1‑140 (MQ=255)
                                         gAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGaa                                                                                                >  1:2237553/1‑140 (MQ=255)
                                         gAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGaa                                                                                                >  1:1842149/1‑140 (MQ=255)
                                          aaaaTCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGGTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGCCATTTTAACGGGGTCTATCGAAc                                                                                               <  2:153547/140‑1 (MQ=255)
                                             aTCGGCAGACACAGACGAGAAACATGTGTATGAAAAGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGCCATTTTAAAGGTGTCTATCGAACCGc                                                                                            <  1:991680/140‑1 (MQ=255)
                                             aTCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGc                                                                                            <  1:1042743/140‑1 (MQ=255)
                                                ggCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTg                                                                                         >  1:1406224/1‑140 (MQ=255)
                                                ggCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTg                                                                                         >  1:2177637/1‑140 (MQ=255)
                                                ggCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTg                                                                                         <  1:2130396/140‑1 (MQ=255)
                                                ggCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGAAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACTTTTTAAAGGTGTCTATCGAACCGCCTg                                                                                         <  1:536985/140‑1 (MQ=255)
                                                    gACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGa                                                                                     <  1:2273533/140‑1 (MQ=255)
                                                    gACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGa                                                                                     <  2:101867/140‑1 (MQ=255)
                                                    gACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGa                                                                                     <  1:839881/140‑1 (MQ=255)
                                                    gACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGGTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGa                                                                                     <  1:643609/140‑1 (MQ=255)
                                                     acacAGACGAGAAAAATGTTTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGAt                                                                                    <  1:1162247/140‑1 (MQ=255)
                                                     acacAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGAt                                                                                    <  2:2177637/140‑1 (MQ=255)
                                                     acacAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGAt                                                                                    <  2:1406224/140‑1 (MQ=255)
                                                     acacAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGAt                                                                                    <  1:1239192/140‑1 (MQ=255)
                                                              agaAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAg                                                                           <  2:333598/140‑1 (MQ=255)
                                                               gaAAAATGTGTATGAAATGTATTACGAGTATGAAGATCCAATTGCAAAAGTAGTTCCGCACATGTTGCTGGCGATGAACCGCGGCTAAAAGGAAGACATTTTAAAGGTGTATATCGAACCGCCTGCGGATCACATTAAgg                                                                          >  2:1946345/1‑140 (MQ=255)
                                                               gaAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAgg                                                                          >  2:240265/1‑140 (MQ=255)
                                                                         tATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGa                                                                >  2:1689453/1‑140 (MQ=255)
                                                                               aTGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTCAGGCGTATTTAGAAAAACa                                                          >  2:2318758/1‑140 (MQ=255)
                                                                               aTGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACa                                                          >  2:2105465/1‑140 (MQ=255)
                                                                                 ggaTTACGAGTATGAAGAGCCACTTGCAAAAGTCGTTCCGCACAGTGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGa                                                        <  2:744650/138‑1 (MQ=255)
                                                                                 gTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGa                                                        >  2:1741862/1‑140 (MQ=255)
                                                                                    ttACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGatca                                                     >  1:888498/1‑140 (MQ=255)
                                                                                    ttACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGatca                                                     >  1:107193/1‑140 (MQ=255)
                                                                                         aGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCaaa                                                >  2:289473/1‑140 (MQ=255)
                                                                                          gTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGgtgt                                                                                                        <  1:1926403/83‑1 (MQ=255)
                                                                                          gTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGgtgt                                                                                                        >  2:1926403/1‑83 (MQ=255)
                                                                                            aTGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGa                                                                                                                      >  2:2251/1‑67 (MQ=255)
                                                                                            aTGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGa                                                                                                                      <  1:2251/67‑1 (MQ=255)
                                                                                            aTGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCg                                                                                       <  2:2125062/98‑1 (MQ=255)
                                                                                            aTGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCg                                                                                       >  1:2125062/1‑98 (MQ=255)
                                                                                               aaGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGc                                          >  2:1220583/1‑140 (MQ=255)
                                                                                                agagCCAATTGCAAAAGTTGTTCCGCATAGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCt                                         <  1:1809671/140‑1 (MQ=255)
                                                                                                    ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGa                                                                                     >  2:830865/1‑92 (MQ=255)
                                                                                                    ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGa                                                                                     <  1:830865/92‑1 (MQ=255)
                                                                                                    ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCt                                         <  1:154831/136‑1 (MQ=255)
                                                                                                    ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                     >  2:2357383/1‑140 (MQ=255)
                                                                                                    ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                     >  2:246343/1‑140 (MQ=255)
                                                                                                    ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                     >  2:136972/1‑140 (MQ=255)
                                                                                                    ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                     >  2:1144730/1‑140 (MQ=255)
                                                                                                    ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                     >  2:35065/1‑140 (MQ=255)
                                                                                                    ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACAAGTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                     >  2:2222603/1‑140 (MQ=255)
                                                                                                     cacttGCAAAAGTCGTTCCGCACACGGTGCTGGCGATGAACCGCGGCGAAATGGAAGACATTGTAAATCTGACTATCGAACCGGCTGCGGATCAAATTAACGCGTATTTAGAACAACATATCATCAAAAACCGCTCAACg                                    >  2:844488/4‑140 (MQ=255)
                                                                                                     cAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGCAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACAGTCCGGCGTATTTAGAAAAACAGATCTTCAAAAACCGCTccagg                                    >  2:1648864/1‑136 (MQ=255)
                                                                                                     cAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTCGAAAAACAGATCATCAAAAACCGCTCAACg                                    >  2:1340868/1‑140 (MQ=255)
                                                                                                      aaTTGCAAAAGTTGTTCCGCATAGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCTCAAcat                                   >  2:933943/1‑138 (MQ=255)
                                                                                                            aaaaGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTaa                             >  1:1376691/1‑140 (MQ=255)
                                                                                                              aaGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTaaaa                           >  1:845804/1‑140 (MQ=255)
                                                                                                                         cataGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCTCAACATCCGTAAGAGAGATTTTGCa                <  1:933943/137‑1 (MQ=255)
                                                                                                                         cataGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCTCAACATCCGTAAGAGAGATTTTGCa                <  1:933982/137‑1 (MQ=255)
                                                                                                                            agtgTGCTTGCGATTAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa             <  2:1905465/137‑1 (MQ=255)
                                                                                                                            aGGGTGCTGTCGATGAACCGCGGCGAAAAGGAAGACATTTTATAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAATCAGATCATCAAAAACCGCTCAACGTCAGTAAAAGAGATTTTGCAAGa             <  2:882024/140‑1 (MQ=255)
                                                                                                                            aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAATGAGATTTTGCAAGa             <  2:595394/140‑1 (MQ=255)
                                                                                                                            aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa             <  2:2231573/140‑1 (MQ=255)
                                                                                                                            aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa             <  1:2115851/140‑1 (MQ=255)
                                                                                                                            aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa             <  1:982986/140‑1 (MQ=255)
                                                                                                                            aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa             <  2:1406555/140‑1 (MQ=255)
                                                                                                                            aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa             <  2:2231671/140‑1 (MQ=255)
                                                                                                                            aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa             <  2:2175640/140‑1 (MQ=255)
                                                                                                                              ggTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTACAAGAGATTTTGCAAGaaa           >  1:591952/1‑140 (MQ=255)
                                                                                                                              ggTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGaaa           >  1:377660/1‑140 (MQ=255)
                                                                                                                                   tGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGa                                                                                     >  1:1986456/1‑61 (MQ=255)
                                                                                                                                   tGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGa                                                                                     <  2:1986456/61‑1 (MQ=255)
                                                                                                                                   tGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGAtt      >  1:2409749/1‑140 (MQ=255)
                                                                                                                                      cGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGaa   <  2:1376691/140‑1 (MQ=255)
                                                                                                                                       gATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAg  <  2:2409749/140‑1 (MQ=255)
                                                                                                                                       gATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAg  <  1:1906061/140‑1 (MQ=255)
                                                                                                                                       gATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAg  <  1:1894440/140‑1 (MQ=255)
                                                                                                                                       gATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAg  <  1:582183/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                           
CCAGGAAACCTTTAAAAGAGGAACCATTAAGTCTACAGCCGGAAAATCGGCAGACACAGACGAGAAAAATGTGTATGAAATGTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAG  >  NZ_CP034943/523253‑523527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: