Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 523,472 G→A 5.2% Q256Q (CAG→CAA EO946_RS02705 → RNA‑binding transcriptional accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943523,4720GA 5.2% 383.0 / 6.8 118Q256Q (CAG→CAAEO946_RS02705RNA‑binding transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base G (61/49);  new base A (2/4);  total (64/54)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 118 total aligned reads displayed.

GTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAGGCCATT  >  NZ_CP034943/523334‑523610
                                                                                                                                          |                                                                                                                                          
ggaTTACGAGTATGAAGAGCCACTTGCAAAAGTCGTTCCGCACAGTGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGa                                                                                                                                           <  2:744650/138‑1 (MQ=255)
gTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGa                                                                                                                                           >  2:29854/1‑140 (MQ=255)
   ttACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGatca                                                                                                                                        >  1:107193/1‑140 (MQ=255)
              aaGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGc                                                                                                                             >  2:1220583/1‑140 (MQ=255)
               agagCCAATTGCAAAAGTTGTTCCGCATAGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCt                                                                                                                            <  1:1809671/140‑1 (MQ=255)
                   ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCt                                                                                                                            >  2:154831/1‑136 (MQ=255)
                   ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCt                                                                                                                            <  1:154831/136‑1 (MQ=255)
                   ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                        >  2:2357383/1‑140 (MQ=255)
                   ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                        >  2:136972/1‑140 (MQ=255)
                   ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                        >  2:246343/1‑140 (MQ=255)
                   ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                        >  2:1144730/1‑140 (MQ=255)
                   ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                        >  2:35065/1‑140 (MQ=255)
                   ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                        >  2:774477/1‑140 (MQ=255)
                   ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACAAGTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                        >  2:2222603/1‑140 (MQ=255)
                    cacttGCAAAAGTCGTTCCGCACACGGTGCTGGCGATGAACCGCGGCGAAATGGAAGACATTGTAAATCTGACTATCGAACCGGCTGCGGATCAAATTAACGCGTATTTAGAACAACATATCATCAAAAACCGCTCAACg                                                                                                                       >  2:844488/4‑140 (MQ=255)
                    cAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGCAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACAGTCCGGCGTATTTAGAAAAACAGATCTTCAAAAACCGCTccagg                                                                                                                       >  2:1648864/1‑136 (MQ=255)
                    cAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTCGAAAAACAGATCATCAAAAACCGCTCAACg                                                                                                                       >  2:1340868/1‑140 (MQ=255)
                     aaTTGCAAAAGTTGTTCCGCATAGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCTCAAcat                                                                                                                      >  2:933943/1‑138 (MQ=255)
                     aaTTGCAAAAGTTGTTCCGCATAGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCTCAAcat                                                                                                                      >  2:933982/1‑138 (MQ=255)
                           aaaaGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTaa                                                                                                                >  1:1376691/1‑140 (MQ=255)
                             aaGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTaaaa                                                                                                              >  1:845804/1‑140 (MQ=255)
                                        cataGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCTCAACATCCGTAAGAGAGATTTTGCa                                                                                                   <  1:933943/137‑1 (MQ=255)
                                        cataGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCTCAACATCCGTAAGAGAGATTTTGCa                                                                                                   <  1:933982/137‑1 (MQ=255)
                                           aGGGTGCTGTCGATGAACCGCGGCGAAAAGGAAGACATTTTATAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAATCAGATCATCAAAAACCGCTCAACGTCAGTAAAAGAGATTTTGCAAGa                                                                                                <  2:882024/140‑1 (MQ=255)
                                           aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAATGAGATTTTGCAAGa                                                                                                <  2:595394/140‑1 (MQ=255)
                                           aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa                                                                                                <  2:1406555/140‑1 (MQ=255)
                                           aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa                                                                                                <  2:2175640/140‑1 (MQ=255)
                                           aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa                                                                                                <  2:1906166/140‑1 (MQ=255)
                                           aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa                                                                                                <  2:2231671/140‑1 (MQ=255)
                                             ggTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTACAAGAGATTTTGCAAGaaa                                                                                              >  1:591952/1‑140 (MQ=255)
                                             ggTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGaaa                                                                                              >  1:377660/1‑140 (MQ=255)
                                                  tGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGAtt                                                                                         >  1:2409749/1‑140 (MQ=255)
                                                     cGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGaa                                                                                      <  2:1376691/140‑1 (MQ=255)
                                                      gATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAg                                                                                     <  2:2409749/140‑1 (MQ=255)
                                                      gATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAg                                                                                     <  1:1906061/140‑1 (MQ=255)
                                                      gATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAg                                                                                     <  1:582183/140‑1 (MQ=255)
                                                      gATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAg                                                                                     <  1:1894440/140‑1 (MQ=255)
                                                       aTGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                        <  2:1813044/104‑1 (MQ=255)
                                                       aTGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                        >  1:1813044/1‑104 (MQ=255)
                                                       aTGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGa                                                                                    >  2:931285/1‑140 (MQ=255)
                                                       aTGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGa                                                                                    >  2:1607811/1‑140 (MQ=255)
                                                       aTGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGa                                                                                    >  2:953145/1‑140 (MQ=255)
                                                       aTGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACAAGAAGGCGTAATTAGAAAAACAGATCATCAAAAACCTCTCAACGACGGTAAAAGAGATATTGATAGAAAAGATTTAAGa                                                                                    >  2:1647661/1‑140 (MQ=255)
                                                        tGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGAc                                                                                   <  2:1842149/140‑1 (MQ=255)
                                                        tGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGAc                                                                                   <  2:55263/140‑1 (MQ=255)
                                                        tGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGAc                                                                                   <  2:1937475/140‑1 (MQ=255)
                                                         gAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGAcc                                                                                  >  2:317793/1‑139 (MQ=255)
                                                          ccccGCGGCGAAAAGTAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATGAGATTAAGTCGTATTTAGAAAAACTGAGGATCAAACACGGCTGATCGTCCGTAAAAGAGATTTTTCAAGAAACGTTTGAagagag                                                                                 <  2:1822893/138‑1 (MQ=255)
                                                          aaCCGCGGCGAAAATGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAg                                                                                 <  1:1832828/140‑1 (MQ=255)
                                                          aaCCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAg                                                                                 <  2:52773/140‑1 (MQ=255)
                                                          aaCCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAg                                                                                 <  1:2302080/140‑1 (MQ=255)
                                                          aaCCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAg                                                                                 <  2:321294/140‑1 (MQ=255)
                                                          aaCCGCGCCGAACAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACACATCCTCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAg                                                                                 <  2:1817722/140‑1 (MQ=255)
                                                               cggcgAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCtata                                                                            >  2:112838/1‑140 (MQ=255)
                                                                ggcgAAATGGAAGACATTTTAAAGTTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATaa                                                                           <  1:73492/140‑1 (MQ=255)
                                                                ggcgAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATaa                                                                           <  2:2421008/140‑1 (MQ=255)
                                                                ggcgAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATaa                                                                           <  1:90550/140‑1 (MQ=255)
                                                                    aaaaGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGa                                                                       <  1:314401/140‑1 (MQ=255)
                                                                          aaGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATc                                                                 <  1:931285/140‑1 (MQ=255)
                                                                              cATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGc                                                             <  1:203822/140‑1 (MQ=255)
                                                                              cATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGc                                                             <  2:2237553/140‑1 (MQ=255)
                                                                              cATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGc                                                             <  2:604193/140‑1 (MQ=255)
                                                                              cATTTTAAAGGTGTCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCAGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGc                                                             <  2:1822003/140‑1 (MQ=255)
                                                                                           tCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCg                                                >  1:550070/1‑140 (MQ=255)
                                                                                            cgaTCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCTCAACATCCGTAAGAGAGATTTTGCAGGAGACAATTGAAGACAGCTATAAAAGGCTGATCCAGCCGGCAATCGAGCGt                                               <  2:176306/138‑1 (MQ=255)
                                                                                                  aaCCGCCTGCGGATCAGATTAAGGCGTATTTAGAAAAACAGATCATCAAAAGCCGCTCAACGTCCGTAAAAGAGATTTGGCACGAAACGATTGAAGAGAGCTATAAAAGACTGAGCCAGCCGGCGATAGAGCGTGAAATc                                         >  1:1396461/1‑140 (MQ=255)
                                                                                                  aaCCGCCTGCGGATCACATTAAGGCGTATTTAGAAACACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATc                                         >  1:1284860/1‑140 (MQ=255)
                                                                                                  aaCCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATc                                         >  1:1837133/1‑140 (MQ=255)
                                                                                                  aaCCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATc                                         >  1:969109/1‑140 (MQ=255)
                                                                                                  aaCCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAACAAACGATTGAAGACAGCTATAAAAGACTGATCCAGACGGCGATAGAGCGTGAAAta                                         >  1:1427547/1‑139 (MQ=255)
                                                                                                       ccTGCGGATCACATTAAGGCGTATTTAGCAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATACAAGACTGATCCAGCCGGCTATAGAGCGTGAAATCAGaaa                                    >  2:393031/1‑140 (MQ=255)
                                                                                                       ccTGCGGATCACATTAAGGCGTATTTAGAAACACAGATCATCAAAAACCGCTCAACGTCCGTCAAAGAGATTTTGCAAGAAACGATGGAAGACAGCTATAAAAGTCTGATCCAGCCGGCGATAGAGCGGGAAATCAGaca                                    >  1:2060461/1‑138 (MQ=255)
                                                                                                       ccTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGc                                                             <  1:2244338/115‑1 (MQ=255)
                                                                                                       ccTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGc                                                             >  2:2244338/1‑115 (MQ=255)
                                                                                                       ccTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGaaa                                    >  2:2269497/1‑140 (MQ=255)
                                                                                                       ccTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGaaa                                    >  1:2247153/1‑140 (MQ=255)
                                                                                                       ccTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGaaa                                    >  2:2372160/1‑140 (MQ=255)
                                                                                                       ccTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGaaa                                    >  1:1890115/1‑140 (MQ=255)
                                                                                                         tGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAag                                  >  1:2129807/1‑140 (MQ=255)
                                                                                                           cGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAagag                                <  1:623289/140‑1 (MQ=255)
                                                                                                                  caTTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATCGAGCGGGAAATCAGAAAAGAGCTGTCAg                         >  2:128246/1‑140 (MQ=255)
                                                                                                                          cGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCg                 <  1:1417853/140‑1 (MQ=255)
                                                                                                                          cGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCg                 <  1:2335218/140‑1 (MQ=255)
                                                                                                                           gTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCgg                <  1:35065/140‑1 (MQ=255)
                                                                                                                            tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGAc                                                                                   <  1:653395/72‑1 (MQ=255)
                                                                                                                            tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGAc                                                                                   >  2:653395/1‑72 (MQ=255)
                                                                                                                            tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAg                                                                                 >  1:2308381/1‑74 (MQ=255)
                                                                                                                            tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAg                                                                                 <  2:2308381/74‑1 (MQ=255)
                                                                                                                            tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGa               >  2:800582/1‑140 (MQ=255)
                                                                                                                            tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGa               >  2:428379/1‑140 (MQ=255)
                                                                                                                            tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGa               >  2:2202216/1‑140 (MQ=255)
                                                                                                                             aTTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGAt              >  2:79089/1‑140 (MQ=255)
                                                                                                                             aTTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGAt              >  2:1219954/1‑140 (MQ=255)
                                                                                                                                 aGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGa                                                                                           >  2:1489008/1‑59 (MQ=255)
                                                                                                                                 aGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGa                                                                                           <  1:1489008/59‑1 (MQ=255)
                                                                                                                                   aaaaaCAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAg        >  2:683357/1‑140 (MQ=255)
                                                                                                                                   aaaaaCAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAg        >  2:112801/1‑140 (MQ=255)
                                                                                                                                   aaaaaCAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAg        >  2:2341696/1‑140 (MQ=255)
                                                                                                                                      aaCAGATCATCAAAAACCGCTCAACGTCCTTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAGGcc     >  2:1542344/1‑140 (MQ=255)
                                                                                                                                      aaCAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAGGcc     >  2:1542330/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GTATTACGAGTATGAAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAGGCCATT  >  NZ_CP034943/523334‑523610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: