Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 523,484 C→T 5.2% N260N (AAC→AAT EO946_RS02705 → RNA‑binding transcriptional accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943523,4840CT 5.2% 373.5 / 6.8 115N260N (AAC→AATEO946_RS02705RNA‑binding transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base C (60/49);  new base T (2/4);  total (62/53)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 115 total aligned reads displayed.

AAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAGGCCATTCACATTTTCT  >  NZ_CP034943/523348‑523620
                                                                                                                                        |                                                                                                                                        
aaGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGc                                                                                                                                       >  2:1220583/1‑140 (MQ=255)
 agagCCAATTGCAAAAGTTGTTCCGCATAGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCt                                                                                                                                      <  1:1809671/140‑1 (MQ=255)
     ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCt                                                                                                                                      <  1:154831/136‑1 (MQ=255)
     ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                                  >  2:35065/1‑140 (MQ=255)
     ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                                  >  2:774477/1‑140 (MQ=255)
     ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                                  >  2:246343/1‑140 (MQ=255)
     ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                                  >  2:1144730/1‑140 (MQ=255)
     ccAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                                  >  2:2357383/1‑140 (MQ=255)
      cacttGCAAAAGTCGTTCCGCACACGGTGCTGGCGATGAACCGCGGCGAAATGGAAGACATTGTAAATCTGACTATCGAACCGGCTGCGGATCAAATTAACGCGTATTTAGAACAACATATCATCAAAAACCGCTCAACg                                                                                                                                 >  2:844488/4‑140 (MQ=255)
      cAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGCAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACAGTCCGGCGTATTTAGAAAAACAGATCTTCAAAAACCGCTccagg                                                                                                                                 >  2:1648864/1‑136 (MQ=255)
      cAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTCGAAAAACAGATCATCAAAAACCGCTCAACg                                                                                                                                 >  2:1340868/1‑140 (MQ=255)
       aaTTGCAAAAGTTGTTCCGCATAGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCTCAAcat                                                                                                                                >  2:933943/1‑138 (MQ=255)
       aaTTGCAAAAGTTGTTCCGCATAGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCTCAAcat                                                                                                                                >  2:933982/1‑138 (MQ=255)
             aaaaGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTaa                                                                                                                          >  1:1376691/1‑140 (MQ=255)
                          cataGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCTCAACATCCGTAAGAGAGATTTTGCa                                                                                                             <  1:933982/137‑1 (MQ=255)
                          cataGGGTGCTGGCAATGAACCGCGGCGAAAAAGAAGACATTTTAAAGGTAGCGATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCTCAACATCCGTAAGAGAGATTTTGCa                                                                                                             <  1:933943/137‑1 (MQ=255)
                             agtgTGCTTGCGATTAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa                                                                                                          <  2:1905465/137‑1 (MQ=255)
                             aGGGTGCTGTCGATGAACCGCGGCGAAAAGGAAGACATTTTATAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAATCAGATCATCAAAAACCGCTCAACGTCAGTAAAAGAGATTTTGCAAGa                                                                                                          <  2:882024/140‑1 (MQ=255)
                             aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAATGAGATTTTGCAAGa                                                                                                          <  2:595394/140‑1 (MQ=255)
                             aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa                                                                                                          <  2:1406555/140‑1 (MQ=255)
                             aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa                                                                                                          <  1:982986/140‑1 (MQ=255)
                             aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa                                                                                                          <  1:2115851/140‑1 (MQ=255)
                             aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa                                                                                                          <  2:1906166/140‑1 (MQ=255)
                             aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa                                                                                                          <  2:2231573/140‑1 (MQ=255)
                             aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa                                                                                                          <  2:2175640/140‑1 (MQ=255)
                             aGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGa                                                                                                          <  2:2231671/140‑1 (MQ=255)
                               ggTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTACAAGAGATTTTGCAAGaaa                                                                                                        >  1:591952/1‑140 (MQ=255)
                                    tGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGAtt                                                                                                   >  1:2409749/1‑140 (MQ=255)
                                       cGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGaa                                                                                                <  2:1376691/140‑1 (MQ=255)
                                        gATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAg                                                                                               <  1:582183/140‑1 (MQ=255)
                                        gATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAg                                                                                               <  1:1906061/140‑1 (MQ=255)
                                        gATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAg                                                                                               <  2:2409749/140‑1 (MQ=255)
                                         aTGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                                  >  1:1813044/1‑104 (MQ=255)
                                         aTGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAAc                                                                                                                                  <  2:1813044/104‑1 (MQ=255)
                                         aTGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGa                                                                                              >  2:1287928/1‑140 (MQ=255)
                                         aTGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGa                                                                                              >  2:953145/1‑140 (MQ=255)
                                         aTGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGa                                                                                              >  2:1607811/1‑140 (MQ=255)
                                         aTGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGa                                                                                              >  2:931285/1‑140 (MQ=255)
                                         aTGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACAAGAAGGCGTAATTAGAAAAACAGATCATCAAAAACCTCTCAACGACGGTAAAAGAGATATTGATAGAAAAGATTTAAGa                                                                                              >  2:1647661/1‑140 (MQ=255)
                                          tGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGAc                                                                                             <  2:1842149/140‑1 (MQ=255)
                                          tGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGAc                                                                                             <  2:1937475/140‑1 (MQ=255)
                                          tGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGAc                                                                                             <  2:55263/140‑1 (MQ=255)
                                           gAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGAcc                                                                                            >  2:317793/1‑139 (MQ=255)
                                            ccccGCGGCGAAAAGTAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATGAGATTAAGTCGTATTTAGAAAAACTGAGGATCAAACACGGCTGATCGTCCGTAAAAGAGATTTTTCAAGAAACGTTTGAagagag                                                                                           <  2:1822893/138‑1 (MQ=255)
                                            aaCCGCGGCGAAAATGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAg                                                                                           <  1:1832828/140‑1 (MQ=255)
                                            aaCCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAACGAGATTTTGCAAGAAACGCTTGAAGACAg                                                                                           <  2:1531351/140‑1 (MQ=255)
                                            aaCCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAg                                                                                           <  2:52773/140‑1 (MQ=255)
                                            aaCCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAg                                                                                           <  1:2302080/140‑1 (MQ=255)
                                            aaCCGCGCCGAACAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACACATCCTCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAg                                                                                           <  2:1817722/140‑1 (MQ=255)
                                                 cggcgAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCtata                                                                                      >  2:112838/1‑140 (MQ=255)
                                                  ggcgAAATGGAAGACATTTTAAAGTTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATaa                                                                                     <  1:73492/140‑1 (MQ=255)
                                                  ggcgAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATaa                                                                                     <  2:2421008/140‑1 (MQ=255)
                                                  ggcgAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATaa                                                                                     <  1:90550/140‑1 (MQ=255)
                                                      aaaaGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGa                                                                                 <  1:314401/140‑1 (MQ=255)
                                                            aaGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATc                                                                           <  1:931285/140‑1 (MQ=255)
                                                                cATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGc                                                                       <  1:203822/140‑1 (MQ=255)
                                                                cATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGc                                                                       <  2:2237553/140‑1 (MQ=255)
                                                                             tCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCg                                                          >  1:1443294/1‑140 (MQ=255)
                                                                             tCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCg                                                          >  1:550070/1‑140 (MQ=255)
                                                                              cgaTCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAAATCATCAAAAATCGCTCAACATCCGTAAGAGAGATTTTGCAGGAGACAATTGAAGACAGCTATAAAAGGCTGATCCAGCCGGCAATCGAGCGt                                                         <  2:176306/138‑1 (MQ=255)
                                                                                    aaCCGCCTGCGGATCAGATTAAGGCGTATTTAGAAAAACAGATCATCAAAAGCCGCTCAACGTCCGTAAAAGAGATTTGGCACGAAACGATTGAAGAGAGCTATAAAAGACTGAGCCAGCCGGCGATAGAGCGTGAAATc                                                   >  1:1396461/1‑140 (MQ=255)
                                                                                    aaCCGCCTGCGGATCACATTAAGGCGTATTTAGAAACACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATc                                                   >  1:1284860/1‑140 (MQ=255)
                                                                                    aaCCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATc                                                   >  1:1837133/1‑140 (MQ=255)
                                                                                    aaCCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATc                                                   >  1:969109/1‑140 (MQ=255)
                                                                                    aaCCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAACAAACGATTGAAGACAGCTATAAAAGACTGATCCAGACGGCGATAGAGCGTGAAAta                                                   >  1:1427547/1‑139 (MQ=255)
                                                                                         ccTGCGGATCACATTAAGGCGTATTTAGCAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATACAAGACTGATCCAGCCGGCTATAGAGCGTGAAATCAGaaa                                              >  2:393031/1‑140 (MQ=255)
                                                                                         ccTGCGGATCACATTAAGGCGTATTTAGAAACACAGATCATCAAAAACCGCTCAACGTCCGTCAAAGAGATTTTGCAAGAAACGATGGAAGACAGCTATAAAAGTCTGATCCAGCCGGCGATAGAGCGGGAAATCAGaca                                              >  1:2060461/1‑138 (MQ=255)
                                                                                         ccTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGc                                                                       <  1:2244338/115‑1 (MQ=255)
                                                                                         ccTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGc                                                                       >  2:2244338/1‑115 (MQ=255)
                                                                                         ccTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGaaa                                              >  2:498088/1‑140 (MQ=255)
                                                                                         ccTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGaaa                                              >  1:1890115/1‑140 (MQ=255)
                                                                                         ccTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGaaa                                              >  2:2269497/1‑140 (MQ=255)
                                                                                         ccTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGaaa                                              >  2:2372160/1‑140 (MQ=255)
                                                                                           tGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAag                                            >  1:2129807/1‑140 (MQ=255)
                                                                                             cGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAagag                                          <  1:623289/140‑1 (MQ=255)
                                                                                                    caTTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATCGAGCGGGAAATCAGAAAAGAGCTGTCAg                                   >  2:128246/1‑140 (MQ=255)
                                                                                                            cGTATTTAGAAAAACAGATCATCAACAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCg                           <  1:553984/140‑1 (MQ=255)
                                                                                                            cGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCg                           <  1:2335218/140‑1 (MQ=255)
                                                                                                            cGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCg                           <  1:1417853/140‑1 (MQ=255)
                                                                                                             gTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCgg                          <  1:35065/140‑1 (MQ=255)
                                                                                                             gTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCgg                          <  1:1863934/140‑1 (MQ=255)
                                                                                                              tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGAc                                                                                             <  1:653395/72‑1 (MQ=255)
                                                                                                              tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGAc                                                                                             >  2:653395/1‑72 (MQ=255)
                                                                                                              tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAg                                                                                           <  2:2308381/74‑1 (MQ=255)
                                                                                                              tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAg                                                                                           >  1:2308381/1‑74 (MQ=255)
                                                                                                              tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGa                         >  2:2202216/1‑140 (MQ=255)
                                                                                                              tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGa                         >  2:800582/1‑140 (MQ=255)
                                                                                                              tATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGa                         >  2:428379/1‑140 (MQ=255)
                                                                                                               aTTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGAt                        >  2:79089/1‑140 (MQ=255)
                                                                                                               aTTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGAt                        >  2:1219954/1‑140 (MQ=255)
                                                                                                                   aGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGa                                                                                                     >  2:1489008/1‑59 (MQ=255)
                                                                                                                     aaaaaCAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAg                  >  2:672370/1‑140 (MQ=255)
                                                                                                                     aaaaaCAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAg                  >  2:683357/1‑140 (MQ=255)
                                                                                                                     aaaaaCAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAg                  >  2:2341696/1‑140 (MQ=255)
                                                                                                                        aaCAGATCATCAAAAACCGCTCAACGTCCTTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAGGcc               >  2:1542344/1‑140 (MQ=255)
                                                                                                                        aaCAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAGGcc               >  2:1542330/1‑140 (MQ=255)
                                                                                                                           aGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGACCTGTCAGAAAAAGCGGATGAGCAGGCCAtt            <  1:235699/140‑1 (MQ=255)
                                                                                                                             atcatcAAAAACCGCTCAACGTCCGTAAAATAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGATCAGGCCATTca          >  1:1149714/1‑140 (MQ=255)
                                                                                                                                     aaaCCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAGGCCATTCACATTTtct  >  2:897143/1‑140 (MQ=255)
                                                                                                                                     aaaCCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAGGCCATTCACATTTtct  >  2:1435595/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
AAGAGCCAATTGCAAAAGTCGTTCCGCACAGGGTGCTGGCGATGAACCGCGGCGAAAAGGAAGACATTTTAAAGGTGTCTATCGAACCGCCTGCGGATCACATTAAGGCGTATTTAGAAAAACAGATCATCAAAAACCGCTCAACGTCCGTAAAAGAGATTTTGCAAGAAACGATTGAAGACAGCTATAAAAGACTGATCCAGCCGGCGATAGAGCGTGAAATCAGAAAAGAGCTGTCAGAAAAAGCGGATGAGCAGGCCATTCACATTTTCT  >  NZ_CP034943/523348‑523620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: