Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,638 G→C 12.0% A161P (GCC→CCC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,6380GC12.0% 189.3 / 5.4 89A161P (GCC→CCC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base G (45/31);  new base C (6/5);  total (53/36)
Fisher's exact test for biased strand distribution p-value = 7.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACT  >  NZ_CP034943/607500‑607775
                                                                                                                                          |                                                                                                                                         
gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                                                          >  2:240913/1‑140 (MQ=255)
gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                                                          >  2:1498623/1‑140 (MQ=255)
gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGagcagc                                                                                                                                          >  2:502042/1‑140 (MQ=255)
  aGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCGAGGGGAACCCGTACCAGCCGGACCGACGGCAGAGCCAGAGGAAGCCGGACCACCCg                                                                                                                                        >  1:1548876/1‑140 (MQ=18)
  aGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCg                                                                                                                                        >  1:1711016/1‑140 (MQ=255)
  aGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCg                                                                                                                                        >  1:1081608/1‑140 (MQ=255)
  aGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGCAGCGACAGGAGCAACCGGGGAAGCCGGACCAGCCg                                                                                                                                        >  1:1770027/1‑140 (MQ=255)
  aGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACAAGCCGCAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCg                                                                                                                                        >  1:1081505/1‑140 (MQ=255)
  aGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGGGCGACGGGAGCCACCGGGGCTCCTGCGGAAGCACGACACGCCGCAGCCTCGGCAGAGACCGCTGAAGACGGCCCAGCCg                                                                                                                                        >  1:855346/1‑140 (MQ=12)
     tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                                                     >  2:2020068/1‑140 (MQ=255)
           aGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                       >  1:1356421/1‑132 (MQ=255)
           aGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                       <  2:1356421/132‑1 (MQ=255)
            gcgggAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                              <  1:1498623/137‑1 (MQ=255)
            gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCAACAGGGGAGCCGGGGGCAGGCGGACCAGCCGGAGCGACAGGAGCTACCGCGGCAGCCGGTCCCGCCGGAGCgccggg                                                                                                                              >  2:2057839/1‑135 (MQ=17)
            gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                              <  1:240913/140‑1 (MQ=255)
             cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                                                             >  1:1541476/1‑140 (MQ=255)
              tGGCGCGACGGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                            <  2:1535571/140‑1 (MQ=255)
                      acGGAGCGCCAGGAGCTCCCGGGGACGCCGGACCAGCCGGCGCCACCGGACCCACAGGCCCGACTGGGGAACCCGGACCACCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                    >  1:780056‑M1/3‑140 (MQ=255)
                        ggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGCCTACTCGGGAAGCCGGACCAGCCCGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGa                                                                                                                  >  1:2078866/1‑140 (MQ=255)
                          aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCGGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                >  2:550942/1‑140 (MQ=255)
                          aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGACGGCGGACCAGCCGGAGCGACAGGAGCAACCGGGGACGCCGGACCAGCCGGAGCGACCGGCGCGACCGGGGacg                                                                                                                >  2:506144/1‑138 (MQ=14)
                                    gCTACCGGGGAAGCCGGACCAGCCGGGGCCAGGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                                      <  1:2235542/140‑1 (MQ=255)
                                     cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                                     <  2:1456674/140‑1 (MQ=255)
                                     cTACCGGCGAAGCCGGACCACCCGGGCCCACGGTAGCCACAGGGGCTACTGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                                     <  2:1456410/140‑1 (MQ=17)
                                       aCCGGGGAAGCCGGAGCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                   <  1:1686167/140‑1 (MQ=255)
                                       aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGCCCAACCgg                                                                                                   >  2:1671975/1‑140 (MQ=255)
                                         cggcgcaGCCGGACCAGCCGGCGCCTCCGGAGCCACAGGGGCTCCTGGTGAAGCCCGAGCTCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                                 <  2:1913676/134‑1 (MQ=14)
                                         cGGTGACGCCGGTCCCGCCGGGGCCCCGGGAGCCCGCGGGGGTACTGGCGAACCCGGACCACCCGGCGCGACAGGTGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                                 <  1:1004893/140‑1 (MQ=14)
                                         cGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                                 <  1:1866904/140‑1 (MQ=255)
                                         cGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                                 <  1:1671866/140‑1 (MQ=255)
                                         cGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                                 <  1:1570131/140‑1 (MQ=255)
                                         cGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                                 <  1:2145640/140‑1 (MQ=255)
                                         cGGGGAACCCGCACCAGCCGGGGCCCCGGGAGCCACCGGGGCTACTGGGGAAGCCGGAGCAGCCGGAGCCACAGGAGCTACCGGGGTAGCCGGACCACCCGGAGCGACAGGAGATACCGGGGAAGCCGGACCAACCGGAg                                                                                                 <  1:445895/140‑1 (MQ=17)
                                           gggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCg                                                                                               <  1:489731/140‑1 (MQ=255)
                                           gcgAAGCCGCACCCCCCGGGGCCACGGGAGCCACAGGGGCTACTTGGGAATCCGGACCCGCCGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCg                                                                                               <  1:1019936/138‑1 (MQ=17)
                                           cggACGCCGGACCCGCCTCGGCCCCCGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCg                                                                                               <  1:5594/139‑1 (MQ=14)
                                            ggAAGCCGGACCAGCCGGTGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGCACCAGCCGGAGCGACAGGAGCTACCGGGTAAGCCGGACAAACCCGAGCGACAGCACCACCCGCCCACGAAGGACCAACCGGACCGa                                                                                              >  1:1780373/1‑140 (MQ=11)
                                            ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGCGAAGCCGTACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCCGACAACCCGGAGCGACAGGAGAGACCGCTGACGCCGGACCAAGCGGAGctc                                                                                              >  1:1759432/1‑138 (MQ=17)
                                            cgAACCCGGACCACCCGGTGCCACGGGAGCCACAGGCGCTCCGGGTGAAGCGGGACCAGCCGGAGCCACAGGACCTACCGGGGAAGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa                                                                                              <  1:1338446/139‑1 (MQ=14)
                                              aaGCCGGCCCCGCCGGGGCCACGGGAGCCACAGGGGCATCTGGGGAAGCCGGACCCCCCGGAGCGACAGGAGCTTCCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                            <  2:1098148/140‑1 (MQ=17)
                                                gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                          >  1:885738/1‑140 (MQ=255)
                                                  cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                           >  2:1445657/1‑121 (MQ=255)
                                                  cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                           <  1:1445657/121‑1 (MQ=255)
                                                     aCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCGGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGCCGGGAGcgc                                                                                     >  1:1928179/1‑138 (MQ=17)
                                                                   cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                       >  1:1140644/1‑140 (MQ=255)
                                                                    gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                      >  1:856374/1‑140 (MQ=255)
                                                                    gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                      >  1:856353/1‑140 (MQ=255)
                                                                         ccACAGGGGCTAGTGGGGAAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCACCCGGAGCGACAGGAGCAACCGGGGAAGCAGGACCAGCCGGAGCGAGAGGAGCTACCTGGGATGCCGGACCAGcc                                                                 >  1:1950356/1‑140 (MQ=11)
                                                                           acaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGCGCGCCAGGAGCTACCGGGGAAGCCGGCCCCGCCgg                                                               >  2:1415659/1‑140 (MQ=14)
                                                                              ggggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                                                                                                  >  1:793307/1‑102 (MQ=40)
                                                                              ggggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                                                                                                  <  2:793307/102‑1 (MQ=40)
                                                                                 gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGCAGAGACCGGGGAACAAGGACCAGCCCGAGAGACACTAGATAAACGGGAATCCGGACCAAGCGTCGAGACAGGAGCGAGCGGGGACGCCGGAGCAGGCGGAGCgcg                                                         >  1:450241/1‑138 (MQ=9)
                                                                                  cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGCACAAGCCGCAGCGACAGCAGCAACCGCGGACGCCGGCCCAACCGGAGCGCCAGGAGCTACCGGGGACGCCGGGCCAGCCGGAGCGCCa                                                        >  1:1848751/1‑140 (MQ=14)
                                                                                  cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCTACCGGGGAAGCAGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGGGACa                                                        >  1:1027558/1‑140 (MQ=255)
                                                                                  cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGACCGACa                                                        >  1:1121096/1‑140 (MQ=255)
                                                                                    aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCg                                                                                                                                   >  1:822610/1‑63 (MQ=40)
                                                                                     cTGGGGAAGCCGGACCAGCCGGAGCGACAGGGGCAACCGGGGAAGCCGGACAATCAGGAGCGACTGGAGCTACCGGGGAAGACTGTGAAACCGCAGGGACAGGAGCAACAGTCGAAGCCGGGGCCGCCGGAGCGACCGGa                                                     >  2:1510186/1‑140 (MQ=2)
                                                                                         ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                 <  2:1140644/140‑1 (MQ=255)
                                                                                         ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGcaa                                                 >  2:1500290/1‑138 (MQ=255)
                                                                                          gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                >  2:1252739/1‑140 (MQ=255)
                                                                                          gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                >  2:1427076/1‑140 (MQ=255)
                                                                                          gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAAGCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCcggagcgaccggagcgac                                                >  2:1667765/1‑140 (MQ=11)
                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAACCCGGACCAGCCGGAGCTACAGGAGCTACCGGCGAAGCCGGACAAACCGGAGCCACAGGAGCTACCGGGGAATCCGGACCAGCCGGACCGACAGGAGCTAcc                                               >  1:257541/1‑140 (MQ=14)
                                                                                             gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                             >  2:1733502/1‑140 (MQ=255)
                                                                                                  aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                        <  1:1427076/140‑1 (MQ=255)
                                                                                                  aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                        <  1:1252739/140‑1 (MQ=255)
                                                                                                              gACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGGAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCAGCCGGACAAGCCGGAGCGCCAGGAGCGACCGGGGAAGCGGGACCAAcct                            >  2:147396/1‑139 (MQ=11)
                                                                                                              gACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGAGAAACCGGACCAAACg                            >  2:605398/1‑140 (MQ=14)
                                                                                                                 aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                         >  2:2401640/1‑140 (MQ=14)
                                                                                                                        aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                  >  1:512266/1‑140 (MQ=255)
                                                                                                                          cGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACAGGGCACTCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGGCCAGCCGGGGCGACGGGAg                >  2:373130/1‑140 (MQ=11)
                                                                                                                          cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAGCGGAGCTACAGCGGACTCAGGACCAGCAGGAGAGACCGGAGCTAGATGGGGCGCCGGGCCACCCGGAGCGACGGGAg                >  2:648700/1‑140 (MQ=11)
                                                                                                                          cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCACCCCGTGCGCCgggcg                >  2:648852/1‑138 (MQ=11)
                                                                                                                           ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                        >  2:1888249/1‑115 (MQ=35)
                                                                                                                           ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                        <  1:1888249/115‑1 (MQ=35)
                                                                                                                           ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCGGACAAGCCGGAGCGACCGGAGCGCCCGGCGAAGCCGGACCAACCGGAGCGACGGGAGc               >  2:2368703/1‑140 (MQ=14)
                                                                                                                              gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCAGGACCAGCCGGAGCGGCCGGAGCGACAGGCGGAGCCGGAGCTCCCGGAGCGCCGGGatcggc            >  1:594663/1‑135 (MQ=11)
                                                                                                                                 gCCGGAGCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCTGAGCGACCGTGGAAGCCGGAGCAGCCGGACCGTCAGGAGCTCCCGCGGACGGCGGAGCACCCGGAGCGACGGGAGCCagagc         >  1:1826764/1‑139 (MQ=11)
                                                                                                                                 gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg         <  1:2401640/140‑1 (MQ=14)
                                                                                                                                 gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg         >  1:652880/1‑140 (MQ=255)
                                                                                                                                 cccGGCCCACCCGCAGCGACCGGCGCTCCCGGCGCAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg         <  1:162936/139‑1 (MQ=14)
                                                                                                                                 cccGGACCGCCAGGAGCGTCCGGCGCTTCCTGTGCCGCCGGACCTACGGGAGCCACAGGCGCTTCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg         <  1:1296364/139‑1 (MQ=14)
                                                                                                                                   ccgACCACCCGGAGCGACCGGCGCTACCGGCGCAGCCGGACCAACCGGCGCTACCGGAGCTCCGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg       <  1:870955/138‑1 (MQ=14)
                                                                                                                                   cGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg       <  2:1633816/140‑1 (MQ=255)
                                                                                                                                   cGGACCCGCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCTACCGGAGCGACTGGAGCTCCCGGTGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg       <  1:1362869/140‑1 (MQ=14)
                                                                                                                                   cGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCTCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg       <  2:2095802/140‑1 (MQ=255)
                                                                                                                                   cGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGCGCAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg       <  1:1718794/140‑1 (MQ=14)
                                                                                                                                   cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg       <  2:885738/140‑1 (MQ=255)
                                                                                                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACCGGAGCCCCCGGACCACCCGGAGCTGCGGGATCCCCAGGGGCTa    >  1:428881/1‑140 (MQ=14)
                                                                                                                                        cAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACt  <  1:1415659/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
GAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACT  >  NZ_CP034943/607500‑607775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑