Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 237,732 T→C 5.0% V56A (GTT→GCT)  EO946_RS01345 → YbeF family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943237,7320TC 5.0% 389.9 / 6.8 119V56A (GTT→GCT) EO946_RS01345YbeF family protein
Reads supporting (aligned to +/- strand):  ref base T (51/62);  new base C (4/2);  total (55/64)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 133 total aligned reads displayed.

TTTTGCCTTTAGGGATTATATTGTTATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTATGGCAACTATATCTGATTATAAT  >  NZ_CP034943/237594‑237869
                                                                                                                                          |                                                                                                                                         
ttttGCCTTTAGGGATTATATTGTTCTCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGgtt                                                                                                                                          <  2:2075638/140‑1 (MQ=255)
ttttGCCTTTAGGGATTATATTGTTATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGgtt                                                                                                                                          <  2:553208/140‑1 (MQ=255)
ttttGCCTTTAGGGATTATATTGTTATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGgtt                                                                                                                                          <  2:1344942/140‑1 (MQ=255)
 tttGCCTTTAGTGATTATATTGTTATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGgttg                                                                                                                                         <  2:1585888/140‑1 (MQ=255)
 tttGCCTTTAGGGATTATATTGTTATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGgttg                                                                                                                                         <  1:826591/140‑1 (MQ=255)
  ttGCCTTTAGGGATTATATTGTTATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGgttgt                                                                                                                                        >  2:950281/1‑140 (MQ=255)
      cTTTAGGGATTATATTGTTATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTTCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGtgttgttgta                                                                                                                                    >  2:1328786/1‑139 (MQ=255)
      cTTTAGGGATTATATTGTTATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTc                                                                                                                                    >  2:2036507/1‑140 (MQ=255)
               ttATATTCTTATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACAATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTg                                                                                                                           >  1:2208474/1‑140 (MQ=255)
                     tGTTATCGGTTGACGGGACTTGTTTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCa                                                                                                                     >  1:892702/1‑140 (MQ=255)
                      gTTATCGGTTGCCGGGACTTTTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCAc                                                                                                                    <  2:560138/140‑1 (MQ=255)
                      gTTATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCAc                                                                                                                    <  2:706328/140‑1 (MQ=255)
                      gTTATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCAc                                                                                                                    <  2:596719/140‑1 (MQ=255)
                       ttATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACt                                                                                                                   >  1:1320220/1‑140 (MQ=255)
                       ttATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACt                                                                                                                   >  1:1767758/1‑140 (MQ=255)
                       ttATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACt                                                                                                                   >  2:200967/1‑140 (MQ=255)
                       ttATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACt                                                                                                                   >  2:85850/1‑140 (MQ=255)
                       ttATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACt                                                                                                                   >  1:1935729/1‑140 (MQ=255)
                       ttATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCACTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACt                                                                                                                   >  1:1025646/1‑140 (MQ=255)
                       ctATCGATTGTCGGGACCTGTATTTGTAAAAACCCGTATCTGATGCCAATGCTTAGTTTGGTGATTTCTCTTGTGCTCACCTTTACCATTTTTAATCAATCATTTTTGGGATGGGCTGTTGTCTATAGCTTGGTTTctct                                                                                                                   >  2:407335/2‑140 (MQ=255)
                       ctATCGATTGTCGGGACCTGTATTTGTAAAAACCCGTATCTGATGCCAATGCTTAGTTTGGTGATTTCTCTTGTGCTCACCTTTACCATTTTTAATCAATCATTTTTGGGATGGGCTGTTGTCTATAGCTTGGTTTctct                                                                                                                   >  2:407032/2‑140 (MQ=255)
                        tATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATATCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTg                                                                                                                  >  2:2333185/1‑140 (MQ=255)
                           cGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCt                                                                                                               <  1:442759/140‑1 (MQ=255)
                           cGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCt                                                                                                               <  1:1211916/140‑1 (MQ=255)
                           cGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCt                                                                                                               <  1:200967/140‑1 (MQ=255)
                              gtGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTa                                                                                                            <  2:1219609/139‑1 (MQ=255)
                                gCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATc                                                                                                          <  2:321878/140‑1 (MQ=255)
                                gCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATc                                                                                                          <  1:2210924/140‑1 (MQ=255)
                                gCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTGAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATc                                                                                                          <  2:674133/140‑1 (MQ=255)
                                 ccGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTGTATAGCTTGGTTTCACTGGCTTTATca                                                                                                         >  1:263071/1‑139 (MQ=255)
                                 ccGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATcc                                                                                                         >  1:1590809/1‑140 (MQ=255)
                                 ccGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATcc                                                                                                         >  1:1118391/1‑140 (MQ=255)
                                  cGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCt                                                                                                        <  2:2415301/140‑1 (MQ=255)
                                  cGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCt                                                                                                        <  1:996150/140‑1 (MQ=255)
                                  cGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCt                                                                                                        <  1:1388622/140‑1 (MQ=255)
                                  cGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCt                                                                                                        <  1:1580383/140‑1 (MQ=255)
                                  cGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCt                                                                                                        <  2:2415558/140‑1 (MQ=255)
                                  cGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCt                                                                                                        <  1:1681342/140‑1 (MQ=255)
                                      aCTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTatat                                                                                                    <  2:178270/140‑1 (MQ=255)
                                      aCTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTatat                                                                                                    <  2:1680177/140‑1 (MQ=255)
                                          gtgtTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATcaca                                                                                                >  1:1095762/1‑140 (MQ=255)
                                          gtgtTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATcaca                                                                                                >  2:1259636/1‑140 (MQ=255)
                                          gtgtTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCGAGTGTTCACTTTTACCCTTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTGATCCtagctccca                                                                                                <  2:848214/140‑8 (MQ=255)
                                          gtgtTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTGGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCATATATcaga                                                                                                >  2:2016563/1‑138 (MQ=255)
                                           tgtTTGTAAAAACGTGTATCTGATGGCGGTGCTTAGTTTGATCATTTCGCTATTGTTCACTTTTACCTTCTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACat                                                                                               <  2:1800070/140‑1 (MQ=255)
                                           tgtTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACat                                                                                               <  2:2051469/140‑1 (MQ=255)
                                              ttGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACattga                                                                                            <  1:2049545/140‑1 (MQ=255)
                                              ttGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCAATTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACattga                                                                                            <  1:1921613/140‑1 (MQ=255)
                                               tGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGgttg                                                                                                                                         <  1:975215/94‑1 (MQ=255)
                                               tGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGgttg                                                                                                                                         >  2:975214/1‑94 (MQ=255)
                                               tGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACattgat                                                                                           >  1:416507/1‑140 (MQ=255)
                                                gTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACattgatt                                                                                          >  2:1485801/1‑140 (MQ=255)
                                                gTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATGTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACattgatt                                                                                          <  2:1181020/140‑1 (MQ=255)
                                                    aaaCGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATCTCACATTGATTGtga                                                                                      <  1:2134750/140‑1 (MQ=255)
                                                           tATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTa                                                                                                            >  1:1649589/1‑111 (MQ=255)
                                                           tATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTa                                                                                                            <  2:1649589/111‑1 (MQ=255)
                                                                  tGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGaggaaaaggaaa                                                                        >  2:1884967/1‑140 (MQ=255)
                                                                   gCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAg                                                                       >  1:2108192/1‑140 (MQ=255)
                                                                      ggTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCAt                                                                    >  1:1549698/1‑140 (MQ=255)
                                                                      ggTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCAt                                                                    >  1:684200/1‑140 (MQ=255)
                                                                       gTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATc                                                                   >  2:742255/1‑140 (MQ=255)
                                                                       gTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATc                                                                   >  2:931670/1‑140 (MQ=255)
                                                                       gTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCCTTATCCTATATCACAGTGATTGTGATGAGGAAAAGGAAAGCATc                                                                   >  2:2389048/1‑140 (MQ=255)
                                                                             aGTTTGATCATTTCTCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGaaa                                                             >  2:868265/1‑140 (MQ=255)
                                                                             aGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCt                                                                                                               >  1:1201104/1‑90 (MQ=255)
                                                                             aGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCt                                                                                                               <  2:1201104/90‑1 (MQ=255)
                                                                                  gATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATaaa                                                        >  1:1817320/1‑140 (MQ=255)
                                                                                    tCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATaaaaa                                                      <  1:782415/140‑1 (MQ=255)
                                                                                    tCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTAATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGTAAAATaaaaa                                                      >  2:2310870/1‑140 (MQ=255)
                                                                                    tCATTTCGCTAGTGTTCACTATTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATCGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATaaaaa                                                      <  1:781541/140‑1 (MQ=255)
                                                                                        ttCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGcc                                                  >  1:1240794/1‑140 (MQ=255)
                                                                                        ttCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGcc                                                  >  1:2069243/1‑140 (MQ=255)
                                                                                           gCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCa                                               <  1:1074831/140‑1 (MQ=255)
                                                                                             tAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTTGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACt                                             <  2:760692/140‑1 (MQ=255)
                                                                                             tAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACt                                             <  1:2239892/140‑1 (MQ=255)
                                                                                             tAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACt                                             <  1:1581624/140‑1 (MQ=255)
                                                                                                  ttCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGaggaa                                                                               <  2:1903077/101‑1 (MQ=255)
                                                                                                  ttCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGaggaa                                                                               >  1:1903077/1‑101 (MQ=255)
                                                                                                    cACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAAc                                      <  1:584587/140‑1 (MQ=255)
                                                                                                    cACTTTTACCATTTTTAACCAATAATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATACAAAATCACATTTATTGTGATGAGGAAAAGGAAAGCATCAGGAAAAAAAAAATGCCCCACTTTACAAc                                      >  1:1510705/1‑140 (MQ=255)
                                                                                                     acctttACCATTTTTAATCAATCATTTTTGGGATGGGCTGTTGTCTATAGCTTGGTTTCTCTGGCTTTATc                                                                                                          >  2:1619311/4‑71 (MQ=255)
                                                                                                     aCTTTTAACATTTTTAACGAATCATTTTTAGGCTGGTTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACa                                     <  2:2277656/140‑1 (MQ=255)
                                                                                                       ttttACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACata                                   >  2:1165943/1‑140 (MQ=255)
                                                                                                         ttACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATc                                                                                                          <  1:1690828/67‑1 (MQ=255)
                                                                                                         ttACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATc                                                                                                          >  2:1690828/1‑67 (MQ=255)
                                                                                                          tACCATTTTTAATCAATCATTTTTGGGATGGGCTGTTGTCTATAGCTTGGTTTCTCTGGCTTTATCCTATATCACATTGATTGTGGTAAGGAAAAGGAAGGAATCAGGAAATTAAAAAGACCTCACTTTCTAACATATCg                                >  2:1041261/1‑140 (MQ=255)
                                                                                                               tttttAACGAATCATTTTTAGGCTGTGTTTTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAATGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGAc                           <  2:636906/140‑1 (MQ=255)
                                                                                                               tttttAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGAc                           <  2:1903105/140‑1 (MQ=255)
                                                                                                                 tttAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTa                         >  2:722752/1‑140 (MQ=255)
                                                                                                                   tAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTATg                       <  2:1772760/140‑1 (MQ=255)
                                                                                                                     aCGAATCATTTTGAGGCTGGGTGGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCGATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTATGGc                     <  2:1592977/140‑1 (MQ=255)
                                                                                                                             tttttAGGCTGTGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTATGGCAACTatat             <  1:1535401/140‑1 (MQ=255)
                                                                                                                             tttttAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTATGGCAACTatat             <  1:62683/140‑1 (MQ=255)
                                                                                                                             tttttAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTATGGCAACTatat             <  1:2036507/140‑1 (MQ=255)
                                                                                                                             tttttAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTATGGCAACTatat             <  1:1328786/140‑1 (MQ=255)
                                                                                                                                  aGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTATGGCAACTATATCTGAt        <  2:1240794/140‑1 (MQ=255)
                                                                                                                                  aGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTATGGCAACTATATCTGAt        <  2:2069243/140‑1 (MQ=255)
                                                                                                                                        ggTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTATGGCAACTATATCTGATTATAAt  >  1:816840/1‑140 (MQ=255)
                                                                                                                                        ggTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTATGGCAACTATATCTGATTATAAt  >  1:175654/1‑140 (MQ=255)
                                                                                                                                        ggTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTATGGCAACTATATCTGATTATAAt  >  1:432876/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TTTTGCCTTTAGGGATTATATTGTTATCGGTTGCCGGGACTTGTGTTTGTAAAAACGTGTATCTGATGCCGGTGCTTAGTTTGATCATTTCGCTAGTGTTCACTTTTACCATTTTTAACGAATCATTTTTAGGCTGGGTTGTTGTCTATAGCTTGGTTTCACTGGCTTTATCCTATATCACATTGATTGTGATGAGGAAAAGGAAAGCATCAGGAAAATAAAAATGCCCCACTTTCCAACATATCGTTGACTATGGCAACTATATCTGATTATAAT  >  NZ_CP034943/237594‑237869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: