Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,688 A→G 10.0% G177G (GGA→GGG EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,6880AG10.0% 205.4 / 13.1 92G177G (GGA→GGGEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (52/29);  new base G (5/4);  total (57/35)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAC  >  NZ_CP034943/607553‑607824
                                                                                                                                       |                                                                                                                                        
aCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCGGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGCCGGGAGcgc                                                                                                                                      >  1:1928179/1‑138 (MQ=17)
              cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                        >  1:1140644/1‑140 (MQ=255)
               gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                       >  1:856374/1‑140 (MQ=255)
               gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                       >  1:856353/1‑140 (MQ=255)
                    ccACAGGGGCTAGTGGGGAAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCACCCGGAGCGACAGGAGCAACCGGGGAAGCAGGACCAGCCGGAGCGAGAGGAGCTACCTGGGATGCCGGACCAGcc                                                                                                                  >  1:1950356/1‑140 (MQ=11)
                      acaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGCGCGCCAGGAGCTACCGGGGAAGCCGGCCCCGCCgg                                                                                                                >  2:1415659/1‑140 (MQ=14)
                            gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGCAGAGACCGGGGAACAAGGACCAGCCCGAGAGACACTAGATAAACGGGAATCCGGACCAAGCGTCGAGACAGGAGCGAGCGGGGACGCCGGAGCAGGCGGAGCgcg                                                                                                          >  1:450241/1‑138 (MQ=9)
                             cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGCACAAGCCGCAGCGACAGCAGCAACCGCGGACGCCGGCCCAACCGGAGCGCCAGGAGCTACCGGGGACGCCGGGCCAGCCGGAGCGCCa                                                                                                         >  1:1848751/1‑140 (MQ=14)
                             cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCTACCGGGGAAGCAGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGGGACa                                                                                                         >  1:1027558/1‑140 (MQ=255)
                             cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGACCGACa                                                                                                         >  1:1121096/1‑140 (MQ=255)
                                cTGGGGAAGCCGGACCAGCCGGAGCGACAGGGGCAACCGGGGAAGCCGGACAATCAGGAGCGACTGGAGCTACCGGGGAAGACTGTGAAACCGCAGGGACAGGAGCAACAGTCGAAGCCGGGGCCGCCGGAGCGACCGGa                                                                                                      >  2:1510186/1‑140 (MQ=2)
                                    ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                  <  2:1140644/140‑1 (MQ=255)
                                    ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGcaa                                                                                                  >  2:1500290/1‑138 (MQ=255)
                                     gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                 >  2:1252739/1‑140 (MQ=255)
                                     gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                 >  2:1427076/1‑140 (MQ=255)
                                     gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAAGCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCcggagcgaccggagcgac                                                                                                 >  2:1667765/1‑140 (MQ=11)
                                      aaGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAACCCGGACCAGCCGGAGCTACAGGAGCTACCGGCGAAGCCGGACAAACCGGAGCCACAGGAGCTACCGGGGAATCCGGACCAGCCGGACCGACAGGAGCTAcc                                                                                                >  1:257541/1‑140 (MQ=14)
                                        gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                              >  2:1733502/1‑140 (MQ=255)
                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                         <  1:1427076/140‑1 (MQ=255)
                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                         <  1:1252739/140‑1 (MQ=255)
                                                         gACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGGAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCAGCCGGACAAGCCGGAGCGCCAGGAGCGACCGGGGAAGCGGGACCAAcct                                                                             >  2:147396/1‑139 (MQ=11)
                                                         gACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGAGAAACCGGACCAAACg                                                                             >  2:605398/1‑140 (MQ=14)
                                                            aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                          >  2:2401640/1‑140 (MQ=14)
                                                                   aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                   >  1:512266/1‑140 (MQ=255)
                                                                     cGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACAGGGCACTCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGGCCAGCCGGGGCGACGGGAg                                                                 >  2:373130/1‑140 (MQ=11)
                                                                     cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAGCGGAGCTACAGCGGACTCAGGACCAGCAGGAGAGACCGGAGCTAGATGGGGCGCCGGGCCACCCGGAGCGACGGGAg                                                                 >  2:648700/1‑140 (MQ=11)
                                                                     cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCACCCCGTGCGCCgggcg                                                                 >  2:648852/1‑138 (MQ=11)
                                                                      ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                         >  2:1888249/1‑115 (MQ=35)
                                                                      ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                         <  1:1888249/115‑1 (MQ=35)
                                                                      ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCGGACAAGCCGGAGCGACCGGAGCGCCCGGCGAAGCCGGACCAACCGGAGCGACGGGAGc                                                                >  2:2368703/1‑140 (MQ=14)
                                                                         gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCAGGACCAGCCGGAGCGGCCGGAGCGACAGGCGGAGCCGGAGCTCCCGGAGCGCCGGGatcggc                                                             >  1:594663/1‑135 (MQ=11)
                                                                            gCCGGAGCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCTGAGCGACCGTGGAAGCCGGAGCAGCCGGACCGTCAGGAGCTCCCGCGGACGGCGGAGCACCCGGAGCGACGGGAGCCagagc                                                          >  1:1826764/1‑139 (MQ=11)
                                                                            gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                          <  1:2401640/140‑1 (MQ=14)
                                                                            gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                          >  1:652880/1‑140 (MQ=255)
                                                                            cccGGCCCACCCGCAGCGACCGGCGCTCCCGGCGCAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                          <  1:162936/139‑1 (MQ=14)
                                                                            cccGGACCGCCAGGAGCGTCCGGCGCTTCCTGTGCCGCCGGACCTACGGGAGCCACAGGCGCTTCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                          <  1:1296364/139‑1 (MQ=14)
                                                                              ccgACCACCCGGAGCGACCGGCGCTACCGGCGCAGCCGGACCAACCGGCGCTACCGGAGCTCCGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                        <  1:870955/138‑1 (MQ=14)
                                                                              cGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                        <  2:1633816/140‑1 (MQ=255)
                                                                              cGGACCCGCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCTACCGGAGCGACTGGAGCTCCCGGTGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                        <  1:1362869/140‑1 (MQ=14)
                                                                              cGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCTCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                        <  2:2095802/140‑1 (MQ=255)
                                                                              cGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGCGCAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                        <  1:1718794/140‑1 (MQ=14)
                                                                              cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                        <  2:885738/140‑1 (MQ=255)
                                                                                 aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACCGGAGCCCCCGGACCACCCGGAGCTGCGGGATCCCCAGGGGCTa                                                     >  1:428881/1‑140 (MQ=14)
                                                                                   cAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACt                                                   <  1:1415659/140‑1 (MQ=255)
                                                                                      ccGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACAAGCCGCAGCGACAGGAGCTACGGGCGACCCCGGCGCAACCCGAGCGACCGGACCCACAGCGGCTACTggg                                                >  2:1923189/1‑140 (MQ=14)
                                                                                      ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCCCAGGGGCTCCTggg                                                >  2:1480988/1‑140 (MQ=255)
                                                                                        ggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAACCCGGCCCAGCCGGAGCGACCGGAGCGACCGGCGCAGCCGGACCACCCGGACCGACGGGAGCCACAGGGCCTACTGGGGa                                              >  2:2106581/1‑140 (MQ=17)
                                                                                          aGCGACAGGAGCTCCCGGGGAAGCAGCACCAACCGGAGCGACAGTAGCTACCGGGGACCCCTGACATACAGGAGCGACAGGAGCTACCGGTGACTCGGGACCAAACGGAGCGCAGGCACCCACAGGGGCGACTGCGGaag                                            >  1:1265645/1‑138 (MQ=9)
                                                                                          aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCACCAGGACCAACCGGAGGGAGATAAGCAACAGGGGCTACGGTGGaag                                            >  2:621312/1‑138 (MQ=14)
                                                                                               cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                       <  1:1733502/140‑1 (MQ=255)
                                                                                               cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCAACGGGGGACCTCGGa                                       >  2:552917/1‑140 (MQ=17)
                                                                                               cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGACGCCGGACCAGCCGGAGCGGCCGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACGCGAGCCACAGGGCCAACGGGGGAACTCGGa                                       >  2:324075/1‑140 (MQ=14)
                                                                                                       aCCGTGGAAGCCGCACCAAACGGAGCGACAGGATCTACCGGGGAACCCGGACCAGCCGGAGCGACAGTAGCTAACGGGTAAGCCGGACAAACCGGAGCGACGGGAGCCAAGTGGGCTACTGGGGAAGTCGGACCAGCCgc                               >  1:2344056/1‑139 (MQ=17)
                                                                                                       aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGTCAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGGCGGGAGCCAGAGGGGCGACTGGGGACGGCTGACGAGCAgg                               >  2:475492/1‑140 (MQ=17)
                                                                                                       aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCTGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGTGACGTCGGACCAGCCgg                               >  1:2164370/1‑140 (MQ=255)
                                                                                                       aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                               >  1:2237140/1‑140 (MQ=255)
                                                                                                         cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCGACGGGCCAGGGCGGAGCAGCGGGAg                             >  2:2053666/1‑140 (MQ=14)
                                                                                                           gggAAGCCGGACCAACCGGAGCGACAGGATCTACAGGGGAAGCAGGACAAGCCGGAGCGAAAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCg                           <  1:2165696/140‑1 (MQ=18)
                                                                                                           gcgcaGCCGGACCAACCCGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCg                           <  1:222530/136‑1 (MQ=255)
                                                                                                             gcaGCCGGCCCTCCCGGCTCGACCGGTGCTTCCGGGGAAGCCGCACCCCCCGGAGCGACAGGTGCTACCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                         <  1:2092632/138‑1 (MQ=17)
                                                                                                             gAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                                                                  <  1:326515/35‑1 (MQ=40)
                                                                                                             gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                >  1:1067748/1‑117 (MQ=255)
                                                                                                             gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                <  2:1067748/117‑1 (MQ=255)
                                                                                                                gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                      <  2:652880/140‑1 (MQ=255)
                                                                                                                accGGAGCGACGGGAGCGACAGGGGCTCCTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGTGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                      <  1:2110028/139‑1 (MQ=17)
                                                                                                                accGGAGCGACGGGAGCCACAGTGGCTACTGGGGACGTCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACGAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGCCGTCGGACCAGCCGGAGCGACAgg                      >  2:1337194/2‑140 (MQ=14)
                                                                                                                 ccGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGa                     <  1:797603/140‑1 (MQ=255)
                                                                                                                 ccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGa                     <  1:197193/140‑1 (MQ=255)
                                                                                                                  gggACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                  <  1:910391/61‑1 (MQ=25)
                                                                                                                  cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                  >  2:910391/1‑62 (MQ=35)
                                                                                                                  cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                       >  2:288880/1‑121 (MQ=255)
                                                                                                                  cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                       <  1:288880/121‑1 (MQ=255)
                                                                                                                   ggACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGc                   >  2:204895/1‑140 (MQ=255)
                                                                                                                     ccccaCCGGAGCGACACGAGCGACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                 <  1:2057839/136‑1 (MQ=18)
                                                                                                                     agccacgggAGCCACAGGAGCTACCGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                 <  1:1373634/133‑1 (MQ=255)
                                                                                                                     aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                 >  1:849396/1‑140 (MQ=255)
                                                                                                                        ccccGGAGCGACAGGAGCTCCCGGGGACTGCGGACCAGCCGGCGCGACAGGACCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTCCTGGCGACGCCGGACCCGCCGGAGCGACAGGAGCTACCg              <  1:2236436/138‑1 (MQ=18)
                                                                                                                        caCCGGAGCGACACGAGCGACCGGGGAATCCGGACCAGCCCGCGCGACAGGACCTACCGTCGAAGCCGTACCCACCGCCGCGACGGGAGCCACCGGGGCTCCTGGCCACCGCGGACCAGCCGGAGCGACAGGAGCTACCg              <  1:609115/139‑1 (MQ=12)
                                                                                                                        aaccccaGCGACAGGAGCTACCGGGGATGGCGGAGCTGCCGGAGCGAGAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACCGGGGAAGACGGACCAGCCGGAGCGACAGGAGCTACCg              <  1:1819478/134‑1 (MQ=17)
                                                                                                                        aaCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCg              <  1:2239152/140‑1 (MQ=255)
                                                                                                                         aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg             >  2:919990/1‑140 (MQ=255)
                                                                                                                         aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg             >  2:1329685/1‑140 (MQ=255)
                                                                                                                          ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGCGCCACTGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGCCGGGCGCGAGCggg            >  2:1988713/1‑140 (MQ=18)
                                                                                                                               gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGGGCTACCGGGGAAGc       >  1:2033716/4‑140 (MQ=18)
                                                                                                                               gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc       >  1:1824714/4‑140 (MQ=18)
                                                                                                                               gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc       >  1:1124912/4‑140 (MQ=18)
                                                                                                                                  aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg    >  2:2218814/1‑140 (MQ=255)
                                                                                                                                  aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg    >  1:1491780/1‑140 (MQ=255)
                                                                                                                                  aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCCACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg    >  2:346566/1‑140 (MQ=18)
                                                                                                                                    aGGGGCTCCGGGGGACGTCGGACCAGCCGGAGCGACTGGAGGTACCGGGGAAGCCGGACCACCCGGAGCGACGTGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc  <  1:648852/140‑1 (MQ=17)
                                                                                                                                    aGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGAAAGGAGCTACGGGGGAAGCCGGCCCACCCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc  <  1:648700/140‑1 (MQ=17)
                                                                                                                                    aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc  <  2:2104753/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
ACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAC  >  NZ_CP034943/607553‑607824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: