BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,694	C→T	16.4%	T179T (ACC→ACT)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,694	0	C	T	16.4%	198.0 / 31.9	105	T179T (ACC→ACT)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base C (51/31); new base T (12/4); total (67/38)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAG  >  NZ_CP034943/607567‑607833

cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                 >  1:1140644/1‑140 (MQ=255)

 gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                                >  1:856374/1‑140 (MQ=255)

 gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                                >  1:856353/1‑140 (MQ=255)

      ccACAGGGGCTAGTGGGGAAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCACCCGGAGCGACAGGAGCAACCGGGGAAGCAGGACCAGCCGGAGCGAGAGGAGCTACCTGGGATGCCGGACCAGcc                                                                                                                           >  1:1950356/1‑140 (MQ=11)

        acaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGCGCGCCAGGAGCTACCGGGGAAGCCGGCCCCGCCgg                                                                                                                         >  2:1415659/1‑140 (MQ=14)

              gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGCAGAGACCGGGGAACAAGGACCAGCCCGAGAGACACTAGATAAACGGGAATCCGGACCAAGCGTCGAGACAGGAGCGAGCGGGGACGCCGGAGCAGGCGGAGCgcg                                                                                                                   >  1:450241/1‑138 (MQ=9)

               cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGCACAAGCCGCAGCGACAGCAGCAACCGCGGACGCCGGCCCAACCGGAGCGCCAGGAGCTACCGGGGACGCCGGGCCAGCCGGAGCGCCa                                                                                                                  >  1:1848751/1‑140 (MQ=14)

               cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCTACCGGGGAAGCAGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGGGACa                                                                                                                  >  1:1027558/1‑140 (MQ=255)

               cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGACCGACa                                                                                                                  >  1:1121096/1‑140 (MQ=255)

                  cTGGGGAAGCCGGACCAGCCGGAGCGACAGGGGCAACCGGGGAAGCCGGACAATCAGGAGCGACTGGAGCTACCGGGGAAGACTGTGAAACCGCAGGGACAGGAGCAACAGTCGAAGCCGGGGCCGCCGGAGCGACCGGa                                                                                                               >  2:1510186/1‑140 (MQ=2)

                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                           <  2:1140644/140‑1 (MQ=255)

                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                          >  2:1427076/1‑140 (MQ=255)

                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                          >  2:1252739/1‑140 (MQ=255)

                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAAGCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCcggagcgaccggagcgac                                                                                                          >  2:1667765/1‑140 (MQ=11)

                        aaGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAACCCGGACCAGCCGGAGCTACAGGAGCTACCGGCGAAGCCGGACAAACCGGAGCCACAGGAGCTACCGGGGAATCCGGACCAGCCGGACCGACAGGAGCTAcc                                                                                                         >  1:257541/1‑140 (MQ=14)

                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                       >  2:1733502/1‑140 (MQ=255)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                  <  1:1252739/140‑1 (MQ=255)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                  <  1:1427076/140‑1 (MQ=255)

                                           gACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGGAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCAGCCGGACAAGCCGGAGCGCCAGGAGCGACCGGGGAAGCGGGACCAAcct                                                                                      >  2:147396/1‑139 (MQ=11)

                                           gACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGAGAAACCGGACCAAACg                                                                                      >  2:605398/1‑140 (MQ=14)

                                                     aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                            >  1:512266/1‑140 (MQ=255)

                                                       cGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACAGGGCACTCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGGCCAGCCGGGGCGACGGGAg                                                                          >  2:373130/1‑140 (MQ=11)

                                                       cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAGCGGAGCTACAGCGGACTCAGGACCAGCAGGAGAGACCGGAGCTAGATGGGGCGCCGGGCCACCCGGAGCGACGGGAg                                                                          >  2:648700/1‑140 (MQ=11)

                                                       cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCACCCCGTGCGCCgggcg                                                                          >  2:648852/1‑138 (MQ=11)

                                                        ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                  <  1:1888249/115‑1 (MQ=35)

                                                        ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                  >  2:1888249/1‑115 (MQ=35)

                                                        ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCGGACAAGCCGGAGCGACCGGAGCGCCCGGCGAAGCCGGACCAACCGGAGCGACGGGAGc                                                                         >  2:2368703/1‑140 (MQ=14)

                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCAGGACCAGCCGGAGCGGCCGGAGCGACAGGCGGAGCCGGAGCTCCCGGAGCGCCGGGatcggc                                                                      >  1:594663/1‑135 (MQ=11)

                                                              gCCGGAGCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCTGAGCGACCGTGGAAGCCGGAGCAGCCGGACCGTCAGGAGCTCCCGCGGACGGCGGAGCACCCGGAGCGACGGGAGCCagagc                                                                   >  1:1826764/1‑139 (MQ=11)

                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                   <  1:2401640/140‑1 (MQ=14)

                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                   >  1:652880/1‑140 (MQ=255)

                                                              cccGGCCCACCCGCAGCGACCGGCGCTCCCGGCGCAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                   <  1:162936/139‑1 (MQ=14)

                                                              cccGGACCGCCAGGAGCGTCCGGCGCTTCCTGTGCCGCCGGACCTACGGGAGCCACAGGCGCTTCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                   <  1:1296364/139‑1 (MQ=14)

                                                                ccgACCACCCGGAGCGACCGGCGCTACCGGCGCAGCCGGACCAACCGGCGCTACCGGAGCTCCGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 <  1:870955/138‑1 (MQ=14)

                                                                cGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 <  2:1633816/140‑1 (MQ=255)

                                                                cGGACCCGCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCTACCGGAGCGACTGGAGCTCCCGGTGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 <  1:1362869/140‑1 (MQ=14)

                                                                cGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCTCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 <  2:2095802/140‑1 (MQ=255)

                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 <  2:885738/140‑1 (MQ=255)

                                                                     cAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACt                                                            <  1:1415659/140‑1 (MQ=255)

                                                                        ccGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACAAGCCGCAGCGACAGGAGCTACGGGCGACCCCGGCGCAACCCGAGCGACCGGACCCACAGCGGCTACTggg                                                         >  2:1923189/1‑140 (MQ=14)

                                                                        ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCCCAGGGGCTCCTggg                                                         >  2:1480988/1‑140 (MQ=255)

                                                                          ggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAACCCGGCCCAGCCGGAGCGACCGGAGCGACCGGCGCAGCCGGACCACCCGGACCGACGGGAGCCACAGGGCCTACTGGGGa                                                       >  2:2106581/1‑140 (MQ=17)

                                                                            aGCGACAGGAGCTCCCGGGGAAGCAGCACCAACCGGAGCGACAGTAGCTACCGGGGACCCCTGACATACAGGAGCGACAGGAGCTACCGGTGACTCGGGACCAAACGGAGCGCAGGCACCCACAGGGGCGACTGCGGaag                                                     >  1:1265645/1‑138 (MQ=9)

                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCACCAGGACCAACCGGAGGGAGATAAGCAACAGGGGCTACGGTGGaag                                                     >  2:621312/1‑138 (MQ=14)

                                                                                 cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                                <  1:1733502/140‑1 (MQ=255)

                                                                                 cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCAACGGGGGACCTCGGa                                                >  2:552917/1‑140 (MQ=17)

                                                                                 cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGACGCCGGACCAGCCGGAGCGGCCGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACGCGAGCCACAGGGCCAACGGGGGAACTCGGa                                                >  2:324075/1‑140 (MQ=14)

                                                                                         aCCGTGGAAGCCGCACCAAACGGAGCGACAGGATCTACCGGGGAACCCGGACCAGCCGGAGCGACAGTAGCTAACGGGTAAGCCGGACAAACCGGAGCGACGGGAGCCAAGTGGGCTACTGGGGAAGTCGGACCAGCCgc                                        >  1:2344056/1‑139 (MQ=17)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGTCAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGGCGGGAGCCAGAGGGGCGACTGGGGACGGCTGACGAGCAgg                                        >  2:475492/1‑140 (MQ=17)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCTGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGTGACGTCGGACCAGCCgg                                        >  1:2164370/1‑140 (MQ=255)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                        >  1:2237140/1‑140 (MQ=255)

                                                                                           cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCGACGGGCCAGGGCGGAGCAGCGGGAg                                      >  2:2053666/1‑140 (MQ=14)

                                                                                             gggAAGCCGGACCAACCGGAGCGACAGGATCTACAGGGGAAGCAGGACAAGCCGGAGCGAAAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCg                                    <  1:2165696/140‑1 (MQ=18)

                                                                                             gcgcaGCCGGACCAACCCGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCg                                    <  1:222530/136‑1 (MQ=255)

                                                                                               gcaGCCGGCCCTCCCGGCTCGACCGGTGCTTCCGGGGAAGCCGCACCCCCCGGAGCGACAGGTGCTACCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                  <  1:2092632/138‑1 (MQ=17)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                                                                           <  1:326515/35‑1 (MQ=40)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                         >  1:1067748/1‑117 (MQ=255)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                         <  2:1067748/117‑1 (MQ=255)

                                                                                                  gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                               <  2:652880/140‑1 (MQ=255)

                                                                                                  accGGAGCGACGGGAGCGACAGGGGCTCCTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGTGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                               <  1:2110028/139‑1 (MQ=17)

                                                                                                  accGGAGCGACGGGAGCCACAGTGGCTACTGGGGACGTCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACGAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGCCGTCGGACCAGCCGGAGCGACAgg                               >  2:1337194/2‑140 (MQ=14)

                                                                                                   ccGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGa                              <  1:797603/140‑1 (MQ=255)

                                                                                                   ccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGa                              <  1:197193/140‑1 (MQ=255)

                                                                                                    gggACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                           <  1:910391/61‑1 (MQ=25)

                                                                                                    cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                           >  2:910391/1‑62 (MQ=35)

                                                                                                    cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                                >  2:288880/1‑121 (MQ=255)

                                                                                                    cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                                <  1:288880/121‑1 (MQ=255)

                                                                                                     ggACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGc                            >  2:204895/1‑140 (MQ=255)

                                                                                                       ccccaCCGGAGCGACACGAGCGACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                          <  1:2057839/136‑1 (MQ=18)

                                                                                                       agccacgggAGCCACAGGAGCTACCGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                          <  1:1373634/133‑1 (MQ=255)

                                                                                                       aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                          >  1:849396/1‑140 (MQ=255)

                                                                                                          ccccGGAGCGACAGGAGCTCCCGGGGACTGCGGACCAGCCGGCGCGACAGGACCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTCCTGGCGACGCCGGACCCGCCGGAGCGACAGGAGCTACCg                       <  1:2236436/138‑1 (MQ=18)

                                                                                                          caCCGGAGCGACACGAGCGACCGGGGAATCCGGACCAGCCCGCGCGACAGGACCTACCGTCGAAGCCGTACCCACCGCCGCGACGGGAGCCACCGGGGCTCCTGGCCACCGCGGACCAGCCGGAGCGACAGGAGCTACCg                       <  1:609115/139‑1 (MQ=12)

                                                                                                          aaccccaGCGACAGGAGCTACCGGGGATGGCGGAGCTGCCGGAGCGAGAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACCGGGGAAGACGGACCAGCCGGAGCGACAGGAGCTACCg                       <  1:1819478/134‑1 (MQ=17)

                                                                                                          aaCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCg                       <  1:2239152/140‑1 (MQ=255)

                                                                                                           aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg                      >  2:1329685/1‑140 (MQ=255)

                                                                                                            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGCGCCACTGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGCCGGGCGCGAGCggg                     >  2:1988713/1‑140 (MQ=18)

                                                                                                                 gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGGGCTACCGGGGAAGc                >  1:2033716/4‑140 (MQ=18)

                                                                                                                 gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                >  1:1824714/4‑140 (MQ=18)

                                                                                                                 gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                >  1:1124912/4‑140 (MQ=18)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg             >  1:1491780/1‑140 (MQ=255)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg             >  2:2218814/1‑140 (MQ=255)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCCACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg             >  2:346566/1‑140 (MQ=18)

                                                                                                                      cggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc           <  1:373130/136‑1 (MQ=17)

                                                                                                                      aGGGGCTCCGGGGGACGTCGGACCAGCCGGAGCGACTGGAGGTACCGGGGAAGCCGGACCACCCGGAGCGACGTGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc           <  1:648852/140‑1 (MQ=17)

                                                                                                                      aGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGAAAGGAGCTACGGGGGAAGCCGGCCCACCCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc           <  1:648700/140‑1 (MQ=17)

                                                                                                                      aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc           <  2:2104753/140‑1 (MQ=255)

                                                                                                                       ggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGTGCTACTGGGGACGTCGCACCTGCCGGAGCGAAAGGAGCTACCGGGGAAGCCGGAcc          >  2:393190/4‑140 (MQ=17)

                                                                                                                       ggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAATCCGGAcc          >  2:1183677/4‑140 (MQ=17)

                                                                                                                       ggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAcc          >  2:1064155/4‑140 (MQ=255)

                                                                                                                       ggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGGAGCAGCCTGAGCGAAAGCAGCTACCGGGGAAGCCGGAcc          >  2:1183909/4‑140 (MQ=17)

                                                                                                                          gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc       >  1:2385585/1‑140 (MQ=255)

                                                                                                                          gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc       >  2:1344266/1‑140 (MQ=255)

                                                                                                                           cTACCGGCGCAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc      <  1:1887097/140‑1 (MQ=255)

                                                                                                                             aCCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                              <  1:1155511/82‑1 (MQ=255)

                                                                                                                             aCCGGGGAAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                              >  2:1155511/1‑82 (MQ=255)

                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg    >  1:2125648/1‑140 (MQ=255)

                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg    >  1:582662/1‑140 (MQ=255)

                                                                                                                               cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCTGACCAACCGGAg  >  1:2403953/1‑140 (MQ=255)

                                                                                                                               cGGGGAAGCCGGACCAGCCGGAGAGACAGGAGCTACCGGGGAAGCCGTACCACCCGGAGCGACGGGATCCACAGGGGCTCCTGGGGACTTCGGAAGAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGTACCAACCGGac  >  1:65623/1‑139 (MQ=18)

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAG  >  NZ_CP034943/607567‑607833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`