BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,700	A→C	30.6%	E181D (GAA→GAC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,700	0	A	C	30.6%	112.6 / 67.5	118	E181D (GAA→GAC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (50/29); new base C (30/7); total (81/37)
Fisher's exact test for biased strand distribution p-value = 8.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 119 total aligned reads displayed.

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAG  >  NZ_CP034943/607567‑607839

cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                       >  1:1140644/1‑140 (MQ=255)

 gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                                      >  1:856374/1‑140 (MQ=255)

 gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                                      >  1:856353/1‑140 (MQ=255)

      ccACAGGGGCTAGTGGGGAAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCACCCGGAGCGACAGGAGCAACCGGGGAAGCAGGACCAGCCGGAGCGAGAGGAGCTACCTGGGATGCCGGACCAGcc                                                                                                                                 >  1:1950356/1‑140 (MQ=11)

        acaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGCGCGCCAGGAGCTACCGGGGAAGCCGGCCCCGCCgg                                                                                                                               >  2:1415659/1‑140 (MQ=14)

               cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGCACAAGCCGCAGCGACAGCAGCAACCGCGGACGCCGGCCCAACCGGAGCGCCAGGAGCTACCGGGGACGCCGGGCCAGCCGGAGCGCCa                                                                                                                        >  1:1848751/1‑140 (MQ=14)

               cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCTACCGGGGAAGCAGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGGGACa                                                                                                                        >  1:1027558/1‑140 (MQ=255)

               cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGACCGACa                                                                                                                        >  1:1121096/1‑140 (MQ=255)

                  cTGGGGAAGCCGGACCAGCCGGAGCGACAGGGGCAACCGGGGAAGCCGGACAATCAGGAGCGACTGGAGCTACCGGGGAAGACTGTGAAACCGCAGGGACAGGAGCAACAGTCGAAGCCGGGGCCGCCGGAGCGACCGGa                                                                                                                     >  2:1510186/1‑140 (MQ=2)

                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                 <  2:1140644/140‑1 (MQ=255)

                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGcaa                                                                                                                 >  2:1500290/1‑138 (MQ=255)

                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                                >  2:1252739/1‑140 (MQ=255)

                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                                >  2:1427076/1‑140 (MQ=255)

                        aaGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAACCCGGACCAGCCGGAGCTACAGGAGCTACCGGCGAAGCCGGACAAACCGGAGCCACAGGAGCTACCGGGGAATCCGGACCAGCCGGACCGACAGGAGCTAcc                                                                                                               >  1:257541/1‑140 (MQ=14)

                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                             >  2:1733502/1‑140 (MQ=255)

                                           gACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGGAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCAGCCGGACAAGCCGGAGCGCCAGGAGCGACCGGGGAAGCGGGACCAAcct                                                                                            >  2:147396/1‑139 (MQ=11)

                                           gACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGAGAAACCGGACCAAACg                                                                                            >  2:605398/1‑140 (MQ=14)

                                                     aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                  >  1:512266/1‑140 (MQ=255)

                                                       cGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACAGGGCACTCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGGCCAGCCGGGGCGACGGGAg                                                                                >  2:373130/1‑140 (MQ=11)

                                                       cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAGCGGAGCTACAGCGGACTCAGGACCAGCAGGAGAGACCGGAGCTAGATGGGGCGCCGGGCCACCCGGAGCGACGGGAg                                                                                >  2:648700/1‑140 (MQ=11)

                                                        ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                        >  2:1888249/1‑115 (MQ=35)

                                                        ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCGGACAAGCCGGAGCGACCGGAGCGCCCGGCGAAGCCGGACCAACCGGAGCGACGGGAGc                                                                               >  2:2368703/1‑140 (MQ=14)

                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCAGGACCAGCCGGAGCGGCCGGAGCGACAGGCGGAGCCGGAGCTCCCGGAGCGCCGGGatcggc                                                                            >  1:594663/1‑135 (MQ=11)

                                                              gCCGGAGCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCTGAGCGACCGTGGAAGCCGGAGCAGCCGGACCGTCAGGAGCTCCCGCGGACGGCGGAGCACCCGGAGCGACGGGAGCCagagc                                                                         >  1:1826764/1‑139 (MQ=11)

                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                         <  1:2401640/140‑1 (MQ=14)

                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                         >  1:652880/1‑140 (MQ=255)

                                                              cccGGCCCACCCGCAGCGACCGGCGCTCCCGGCGCAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                         <  1:162936/139‑1 (MQ=14)

                                                              cccGGACCGCCAGGAGCGTCCGGCGCTTCCTGTGCCGCCGGACCTACGGGAGCCACAGGCGCTTCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                         <  1:1296364/139‑1 (MQ=14)

                                                                ccgACCACCCGGAGCGACCGGCGCTACCGGCGCAGCCGGACCAACCGGCGCTACCGGAGCTCCGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                       <  1:870955/138‑1 (MQ=14)

                                                                cGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                       <  2:1633816/140‑1 (MQ=255)

                                                                cGGACCCGCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCTACCGGAGCGACTGGAGCTCCCGGTGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                       <  1:1362869/140‑1 (MQ=14)

                                                                cGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCTCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                       <  2:2095802/140‑1 (MQ=255)

                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGCGCAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                       <  1:1718794/140‑1 (MQ=14)

                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                       <  2:885738/140‑1 (MQ=255)

                                                                   aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACCGGAGCCCCCGGACCACCCGGAGCTGCGGGATCCCCAGGGGCTa                                                                    >  1:428881/1‑140 (MQ=14)

                                                                     cAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACt                                                                  <  1:1415659/140‑1 (MQ=255)

                                                                        ccGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACAAGCCGCAGCGACAGGAGCTACGGGCGACCCCGGCGCAACCCGAGCGACCGGACCCACAGCGGCTACTggg                                                               >  2:1923189/1‑140 (MQ=14)

                                                                        ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCCCAGGGGCTCCTggg                                                               >  2:1480988/1‑140 (MQ=255)

                                                                          ggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAACCCGGCCCAGCCGGAGCGACCGGAGCGACCGGCGCAGCCGGACCACCCGGACCGACGGGAGCCACAGGGCCTACTGGGGa                                                             >  2:2106581/1‑140 (MQ=17)

                                                                            aGCGACAGGAGCTCCCGGGGAAGCAGCACCAACCGGAGCGACAGTAGCTACCGGGGACCCCTGACATACAGGAGCGACAGGAGCTACCGGTGACTCGGGACCAAACGGAGCGCAGGCACCCACAGGGGCGACTGCGGaag                                                           >  1:1265645/1‑138 (MQ=9)

                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCACCAGGACCAACCGGAGGGAGATAAGCAACAGGGGCTACGGTGGaag                                                           >  2:621312/1‑138 (MQ=14)

                                                                                 cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCAACGGGGGACCTCGGa                                                      >  2:552917/1‑140 (MQ=17)

                                                                                 cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGACGCCGGACCAGCCGGAGCGGCCGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACGCGAGCCACAGGGCCAACGGGGGAACTCGGa                                                      >  2:324075/1‑140 (MQ=14)

                                                                                         aCCGTGGAAGCCGCACCAAACGGAGCGACAGGATCTACCGGGGAACCCGGACCAGCCGGAGCGACAGTAGCTAACGGGTAAGCCGGACAAACCGGAGCGACGGGAGCCAAGTGGGCTACTGGGGAAGTCGGACCAGCCgc                                              >  1:2344056/1‑139 (MQ=17)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCTGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGTGACGTCGGACCAGCCgg                                              >  1:2164370/1‑140 (MQ=255)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                              >  1:2237140/1‑140 (MQ=255)

                                                                                             gcgcaGCCGGACCAACCCGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCg                                          <  1:222530/136‑1 (MQ=255)

                                                                                               gcaGCCGGCCCTCCCGGCTCGACCGGTGCTTCCGGGGAAGCCGCACCCCCCGGAGCGACAGGTGCTACCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                        <  1:2092632/138‑1 (MQ=17)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                               >  1:1067748/1‑117 (MQ=255)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                               <  2:1067748/117‑1 (MQ=255)

                                                                                                  gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                     <  2:652880/140‑1 (MQ=255)

                                                                                                  accGGAGCGACGGGAGCGACAGGGGCTCCTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGTGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                     <  1:2110028/139‑1 (MQ=17)

                                                                                                  accGGAGCGACGGGAGCCACAGTGGCTACTGGGGACGTCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACGAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGCCGTCGGACCAGCCGGAGCGACAgg                                     >  2:1337194/2‑140 (MQ=14)

                                                                                                   ccGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGa                                    <  1:797603/140‑1 (MQ=255)

                                                                                                   ccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGa                                    <  1:197193/140‑1 (MQ=255)

                                                                                                    gggACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                 <  1:910391/61‑1 (MQ=25)

                                                                                                    cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                 >  2:910391/1‑62 (MQ=35)

                                                                                                    cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                                      >  2:288880/1‑121 (MQ=255)

                                                                                                    cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                                      <  1:288880/121‑1 (MQ=255)

                                                                                                     ggACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGc                                  >  2:204895/1‑140 (MQ=255)

                                                                                                       ccccaCCGGAGCGACACGAGCGACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                                <  1:2057839/136‑1 (MQ=18)

                                                                                                       agccacgggAGCCACAGGAGCTACCGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                                <  1:1373634/133‑1 (MQ=255)

                                                                                                          ccccGGAGCGACAGGAGCTCCCGGGGACTGCGGACCAGCCGGCGCGACAGGACCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTCCTGGCGACGCCGGACCCGCCGGAGCGACAGGAGCTACCg                             <  1:2236436/138‑1 (MQ=18)

                                                                                                          caCCGGAGCGACACGAGCGACCGGGGAATCCGGACCAGCCCGCGCGACAGGACCTACCGTCGAAGCCGTACCCACCGCCGCGACGGGAGCCACCGGGGCTCCTGGCCACCGCGGACCAGCCGGAGCGACAGGAGCTACCg                             <  1:609115/139‑1 (MQ=12)

                                                                                                          aaccccaGCGACAGGAGCTACCGGGGATGGCGGAGCTGCCGGAGCGAGAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACCGGGGAAGACGGACCAGCCGGAGCGACAGGAGCTACCg                             <  1:1819478/134‑1 (MQ=17)

                                                                                                          aaCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCg                             <  1:2239152/140‑1 (MQ=255)

                                                                                                           aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg                            >  2:1329685/1‑140 (MQ=255)

                                                                                                            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGCGCCACTGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGCCGGGCGCGAGCggg                           >  2:1988713/1‑140 (MQ=18)

                                                                                                                 gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGGGCTACCGGGGAAGc                      >  1:2033716/4‑140 (MQ=18)

                                                                                                                 gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                      >  1:1124912/4‑140 (MQ=18)

                                                                                                                 gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                      >  1:1824714/4‑140 (MQ=18)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                   >  1:1491780/1‑140 (MQ=255)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                   >  2:2218814/1‑140 (MQ=255)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCCACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                   >  2:346566/1‑140 (MQ=18)

                                                                                                                      cggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                 <  1:373130/136‑1 (MQ=17)

                                                                                                                      aGGGGCTCCGGGGGACGTCGGACCAGCCGGAGCGACTGGAGGTACCGGGGAAGCCGGACCACCCGGAGCGACGTGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                 <  1:648852/140‑1 (MQ=17)

                                                                                                                      aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                 <  2:2104753/140‑1 (MQ=255)

                                                                                                                       ggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGTGCTACTGGGGACGTCGCACCTGCCGGAGCGAAAGGAGCTACCGGGGAAGCCGGAcc                >  2:393190/4‑140 (MQ=17)

                                                                                                                       ggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAATCCGGAcc                >  2:1183677/4‑140 (MQ=17)

                                                                                                                       ggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAcc                >  2:1064112/4‑140 (MQ=255)

                                                                                                                       ggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGGAGCAGCCTGAGCGAAAGCAGCTACCGGGGAAGCCGGAcc                >  2:1183909/4‑140 (MQ=17)

                                                                                                                          gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc             >  1:2133684/1‑140 (MQ=255)

                                                                                                                          gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc             >  2:1344266/1‑140 (MQ=255)

                                                                                                                           cTACCGGCGCAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc            <  1:1887097/140‑1 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGCCCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  1:834318/4‑140 (MQ=17)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCgg          >  2:59208/4‑140 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  1:2153527/4‑140 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  1:1740637/4‑140 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  1:1726539/4‑140 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  2:751087/4‑140 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGACGCACCAGCCGGAGCGAAAGCAGCTACCGGGGAACCCGGACAAACCgc          >  2:65631/4‑139 (MQ=17)

                                                                                                                             aCCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                                    <  1:1155511/82‑1 (MQ=255)

                                                                                                                             aCCGGGGAAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                                    >  2:1155511/1‑82 (MQ=255)

                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  1:582662/1‑140 (MQ=255)

                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  1:2125648/1‑140 (MQ=255)

                                                                                                                               cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCTGACCAACCGGAg        >  1:2403953/1‑140 (MQ=255)

                                                                                                                               cGGGGAAGCCGGACCAGCCGGAGAGACAGGAGCTACCGGGGAAGCCGTACCACCCGGAGCGACGGGATCCACAGGGGCTCCTGGGGACTTCGGAAGAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGTACCAACCGGac        >  1:65623/1‑139 (MQ=18)

                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc    >  1:1511122/1‑140 (MQ=255)

                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc    >  1:456802/1‑140 (MQ=255)

                                                                                                                                     aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAcgg  >  2:2056908/1‑138 (MQ=255)

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAG  >  NZ_CP034943/607567‑607839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`