BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,847	G→A	12.2%	T230T (ACG→ACA)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,847	0	G	A	12.2%	657.8 / 58.8	243	T230T (ACG→ACA)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base G (88/123); new base A (6/23); total (95/148)
Fisher's exact test for biased strand distribution p-value = 4.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

GCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGC  >  NZ_CP034943/607710‑607981

gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACagg                                                                                                                                      <  1:550942/140‑1 (MQ=255)

    gAGCGACTGGCGCTACCGGGGAAGCCGCACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGcc                                                                                                                                  <  1:621312/140‑1 (MQ=17)

          cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCCCGGGAGCcacaggc                                                                                                                            >  2:300873/1‑140 (MQ=255)

          cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGg                                                                                                                            >  2:512266‑M2/1‑139 (MQ=255)

           aGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCCACGGGAGCaacaggca                                                                                                                           >  1:1711496/1‑140 (MQ=255)

            ggtgCTAGCGGGGCTCCCGGCCCAACCGGAGCGACGCGATCCCCAGGGGCTACCGGGGACTCCGGCACACCCGGAGCGACAGGAGCTTCCGGGGACGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                                                                                          <  2:2344056/137‑1 (MQ=18)

            ggAGCTACGGGGGACGCCGCAACAACCTGAGAGACGGGAGCCACCGGCGCTACCGGGTAAGTCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                                                                                          <  2:2164370/140‑1 (MQ=17)

              cgcTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacg                                                                                                                        >  1:1988713‑M2/2‑135 (MQ=255)

               gCTACCGGCGAAGCCGGAGCAACCGGAGCGTCGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                       <  1:1923189/140‑1 (MQ=255)

                 tACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGag                                                                                                                     <  1:1329685/140‑1 (MQ=255)

                  aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGggccgtcgg                                                                                                                    <  2:2409003‑M2/140‑10 (MQ=255)

                  aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagc                                                                                                                    >  2:1540483/1‑140 (MQ=255)

                    cGGGGCACCCGGACCCCCCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGCCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcag                                                                                                                  <  1:2175715/140‑1 (MQ=18)

                    cGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggac                                                                                                                  <  2:2319399‑M2/140‑12 (MQ=255)

                    cGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCCACGGGGGCtactgggacagtcggac                                                                                                                  >  2:377563/1‑134 (MQ=17)

                       ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTg                                                                                                               >  1:580277/1‑140 (MQ=255)

                        gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGCCGTCGCACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACTGGAGCCACGGGGGCTACCGGggacgtcgcagcagc                                                                                                              <  2:436225‑M2/140‑16 (MQ=255)

                        gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccagc                                                                                                              <  2:1859678‑M2/140‑16 (MQ=255)

                           gCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGtt                                                                                                           >  1:1386868/1‑140 (MQ=255)

                                aCCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGcgaagtcggaccagccggagcga                                                                                                      <  1:1116132‑M2/140‑24 (MQ=255)

                                   ccccGGCGCGCCCGGAGCGACCGGGGCAGCTGGGGACGCCGGACCCCCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACGGGAGCAGCTGGGTTATCCGAAt                                                                                                   <  2:2048432/138‑1 (MQ=21)

                                    gccGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                  <  2:580277/139‑1 (MQ=255)

                                    cccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                  <  1:502042/139‑1 (MQ=255)

                                    aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACagg                                                                                                                                      >  2:1998880/1‑104 (MQ=255)

                                    aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCtactgg                                                                                                                             <  2:885239/113‑1 (MQ=14)

                                    aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccagccggagcgacagg                                                                                                  <  2:1640120‑M2/140‑28 (MQ=255)

                                    aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccagccggagcgacagg                                                                                                  <  2:223312‑M2/140‑28 (MQ=255)

                                    aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccagccggagcgacagg                                                                                                  <  2:1445217‑M2/140‑28 (MQ=255)

                                    aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                  <  1:982802/140‑1 (MQ=255)

                                    aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                  <  1:1540483/140‑1 (MQ=255)

                                    aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                  <  1:2201278/140‑1 (MQ=255)

                                     ccGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCCACAGGGGCGACAGGggacgtcgcaccagccgtagcgacagga                                                                                                 <  2:213288‑M2/140‑29 (MQ=255)

                                     ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccagccggagcgacagga                                                                                                 <  1:516160‑M2/140‑29 (MQ=255)

                                     ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacggcggagcagccggagcgacagga                                                                                                 <  2:589884‑M2/140‑29 (MQ=255)

                                     ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacggcggaccagcctgagcgacagga                                                                                                 <  2:2268189‑M2/140‑29 (MQ=255)

                                         aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                             <  2:1491780/140‑1 (MQ=255)

                                         aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                             <  1:1064155/140‑1 (MQ=255)

                                         aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                             <  1:1183677/140‑1 (MQ=255)

                                         aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                             <  1:1640120/140‑1 (MQ=255)

                                            gACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtata                                                                                          <  1:204895/140‑1 (MQ=255)

                                              cGGGAGCCTCTGGGGCTACTGGGGCCGGCGGACCAGCCGGAGCGTATGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatata                                                                                        <  2:1116132/140‑1 (MQ=25)

                                              cGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTCTCCGAATACGGGtatata                                                                                        >  1:1975771/1‑140 (MQ=255)

                                                ggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatatata                                                                                      >  1:431785/1‑140 (MQ=255)

                                                       gaGGGGGTACGGGGGTCTGGGGAACAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTa                                                                               <  1:2019694/139‑1 (MQ=25)

                                                          gggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACTGGCACAGGAGCAGCTGGGTTATCCGAATCCGGGTCTATCTATCACTTAAgc                                                                            >  2:714438/1‑140 (MQ=25)

                                                          gggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAgc                                                                            >  2:1172358/1‑140 (MQ=255)

                                                            gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                                                                                          <  2:1096835/92‑1 (MQ=255)

                                                            gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                                                                                          >  1:1096835/1‑92 (MQ=255)

                                                            gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAgcgc                                                                          >  2:1279198/1‑140 (MQ=255)

                                                            gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGATATCCGAATACGGGTATATATATCACTTAAgcgc                                                                          >  2:1043340/1‑140 (MQ=255)

                                                               aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcagc                                                                                                                 <  2:863410/98‑1 (MQ=255)

                                                               aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                       <  1:1136233/140‑1 (MQ=255)

                                                               aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                       >  2:1955002/1‑140 (MQ=255)

                                                               aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                       >  2:742108/1‑140 (MQ=255)

                                                                cTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTggg                                                                                                             >  2:268777/1‑101 (MQ=255)

                                                                     gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCCACGGGAGCCACAGGCACAGCAGCAGCTGGGTTATCCGAATACGGGTAGAGATATAACTTAAGAGCTCAAACGGt                                                                 >  2:1632752/1‑140 (MQ=37)

                                                                     gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACTGGCACAGGAGCAGCTGGTTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                 <  1:714438/140‑1 (MQ=255)

                                                                     gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                 >  1:78837/1‑140 (MQ=255)

                                                                     gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                 >  1:834517/1‑140 (MQ=255)

                                                                      aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                       <  1:876186/85‑1 (MQ=255)

                                                                      aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                                <  1:1603940/140‑1 (MQ=255)

                                                                      aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                                >  1:1215113/1‑140 (MQ=255)

                                                                      aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                                >  1:1277004/1‑140 (MQ=255)

                                                                      aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                                >  1:1791586/1‑140 (MQ=255)

                                                                       agTCGGACCTCCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAg                                                               <  1:1234339/139‑1 (MQ=255)

                                                                        gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGc                                                              >  1:757099/1‑140 (MQ=255)

                                                                        gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGc                                                              >  1:2400273/1‑140 (MQ=255)

                                                                        gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGc                                                              <  1:500773/140‑1 (MQ=255)

                                                                          cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                  <  1:2340864/102‑1 (MQ=255)

                                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                       <  1:926281/78‑1 (MQ=255)

                                                                                  ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAAc                                                                    <  1:629904/124‑1 (MQ=255)

                                                                                  ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAAc                                                                    >  2:629904/1‑124 (MQ=255)

                                                                                  ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCt                                                    >  1:96662/1‑140 (MQ=255)

                                                                                       gccaCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                  >  2:561419/4‑89 (MQ=255)

                                                                                               aGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacagg                                                                                                                             >  2:1563222/1‑54 (MQ=255)

                                                                                                gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGa                                      <  2:964392/140‑1 (MQ=255)

                                                                                                   aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCCACAGGCACAGGAGCAGCAGGGTTATCCGAATAAGGTTCGATCTATCACTTAAGCGCACAAACGCTCGCTCTAGAGGCTGATGTCAGTTTTGATTc                                   >  1:1867746/1‑140 (MQ=25)

                                                                                                     cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                                 <  1:446920/140‑1 (MQ=255)

                                                                                                              cGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATaa                        >  2:786695/1‑140 (MQ=255)

                                                                                                              cGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATaa                        <  1:323931/140‑1 (MQ=255)

                                                                                                               ggACCACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGCGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                       <  1:1461840/140‑1 (MQ=255)

                                                                                                               ggACCAACCGGATCGACAGTAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATTTCATTTTTGATTCAACAGGGATAac                       <  1:33507/140‑1 (MQ=255)

                                                                                                               ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                       <  1:589884/140‑1 (MQ=255)

                                                                                                               ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                       <  1:1508807/140‑1 (MQ=255)

                                                                                                               ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                       <  1:2106581/140‑1 (MQ=255)

                                                                                                               ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                       <  1:2032344/140‑1 (MQ=255)

                                                                                                                 aCCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAacca                     >  2:591107/1‑140 (MQ=255)

                                                                                                                     aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                 <  1:1445217/140‑1 (MQ=255)

                                                                                                                     aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                 <  1:897911/140‑1 (MQ=255)

                                                                                                                     aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                 <  1:2319399/140‑1 (MQ=255)

                                                                                                                     aCCGGAGCGACAGGAGCCAAGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                 <  2:1265645/140‑1 (MQ=255)

                                                                                                                           gccaCAGGAGCCACGGGAGCCACCGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTAGATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGaag           <  1:300873/137‑2 (MQ=255)

                                                                                                                              aCAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTac        >  1:1023692/1‑140 (MQ=255)

                                                                                                                                 ggAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca     <  1:751087/140‑1 (MQ=255)

                                                                                                                                 ggAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca     <  1:1309442/140‑1 (MQ=255)

                                                                                                                                  gAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACAt    <  1:1156852/140‑1 (MQ=255)

                                                                                                                                  gAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACAt    <  1:38710/140‑1 (MQ=255)

                                                                                                                                    gCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCAtt                                           >  2:1901686/1‑99 (MQ=255)

                                                                                                                                         gggAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca     <  1:2119647/132‑1 (MQ=255)

                                                                                                                                         gggAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca     >  2:2119647/1‑132 (MQ=255)

GCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGC  >  NZ_CP034943/607710‑607981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`